• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.117-123
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• 2000

The object of this work is to improve the maintenance of bioluminescence from stored Photobacterium phosphoreum in a view of developing continuous monitoring system for pollutants. The long-term experiments were performed to determine the effect of storage temperature and immobilization on the maintenance of bioluminescence and viability of P. phosphoreum. A naturally luminescent bacterium, P. phosphoreum was starved in 2.5% Nael solution at $20^{\circ}C$, $4^{\circ}C$, -$20^{\circ}C$ and -$70^{\circ}C$ for 30 days. In vivo luminescence was measured by luminometry, and total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively. The bioluminescence emission from cells stored at 4De was maintained up to 10 days while those with starved cells at other temperature ranges decreased to background level within 3 days. In terms of viability of cells, concentrations of cells stored at $20^{\circ}C$ were rapidly decreased as a result of cell lysis, leading to a drop in culturable and viable counts while cells stored at $4^{\circ}C$ was shown viable but nonculturable state during starvation. With immobilized cells on strontium alginate, the bioluminescence showed higher maintenance than free cells and decreased with count number of nonculturable cells.

• Journal of the Korean association of regional geographers
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• v.14 no.6
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• pp.643-663
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• 2008

The rural area of S. Korea has been excluded from the process of industrialization and urbanization, and is to be faced with a serious crisis in the context of recent globalization/localization with a rapid economic and political change. And hence it is required to give a active attention to community-based welfare for rural areas. The status of welfare of Koryung-gun as such a rural area adjacent to Daegu city is still low, even though recently it has been improved dramatically, and the welfare policy seems not to reflect the rapidly changing conditions of reality, though it has been institutionalized systematically. What is more, the number of dwellers who demand basic welfare has been increased, as welfare services seem not to delivered to them. In order to improve this totally low level of social welfare, major tasks should be pursued to formulate as basic principles an integral approach for community-based welfare and ultimately a restoration of rural community; to construct a mobilization system of diverse resources within community, to develop connections between welfare demanders and providers, and networks of institutes and facilities for community welfare; and to find out and to extend financial sources for welfare policies.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.10
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• pp.194-205
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• 2018

This study investigated knowledge and attitudes of long-term care hospital nursing assistants such as nursing aides and direct care workers toward patient safety and their safety care activities. This was a descriptive study that attempted to enhance the competence of nursing assistants' patient safety management, and the data were used to develop a training program for nursing assistants. A total of 230 nursing assistants working at six hospitals whose wards were over 200 participated and were assessed with a rating 1 or 2 in 2013 in B city. Data were collected using questionnaires from March 2 to March 26 in 2017. The collected data were analyzed by the SPSS/WIN 20.0 program using number, percentage, mean, standard deviation, t-test, ANOVA, $Scheff{\acute{e}}$ test, correlation coefficient, and multiple regression. In conclusion, the findings show that patient safety care activities were better performed by nursing aides than direct care workers. Higher education and a higher number of patient safety training programs and patient safety care activities were frequent. In addition, nursing assistants' knowledge of patient safety affected attitudes toward patient safety, and attitudes affected patient safety care activities. Therefore, an actual regular training program for patient safety should be executed over five times a year to improve patient safety perception. Furthermore, a hospital system including adequate working time needs to be improved. Based on the results of the study, patient safety in long-term care hospitals should be improved, and this study could be utilized as basic data to develop a training course for improvement of knowledge and attitudes of nursing assistants toward patient safety.

• Journal of Biosystems Engineering
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• v.36 no.5
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• pp.326-333
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• 2011

The need for rapid on-site monitoring of hydroponic macronutrients has led to the use of ion-selective electrodes, because of their advantages over spectrophotometric methods, including simple methodology, direct measurement of analyte, sensitivity over a wide concentration range, and low cost. Stability and repeatability of response can be a concern when using multiple ion-selective electrodes to measure concentrations in a series of samples because accuracy might be limited by drifts in electrode potential. A computer-based measurement system could improve accuracy and precision because of both consistent control of sample preparation and easy calibration of sensors. Our goal was to investigate the applicability of a cobalt-based electrode used in conjunction with a laboratory-made automated test stand for quantitative determination of ${PO_4}^-$ in hydroponic solution. Six hydroponic solutions were prepared by diluting highly concentrated paprika hydroponicsolution to provide a concentration range of 1 to 300 ppm $PO_4$-P. A calibration curve relating electrode response to phosphate in paprika hydroponic solution titrated to pH 4 with 0.025M KHP was developed based on the Nikolskii-Eisenman equation with a coefficient of determination ($R^2$) of 0.94. The laboratory-made test stand consisting of three cobalt-based electrodes measured phosphate concentrations similar to those obtained with standard laboratory methods (a regression slope of 0.98 with $R^2$ = 0.80). However, the y intercept was relatively high, 30 ppm, probably due to the relatively large amount of variation present among multiple measurements of the same sample. Further studies on the high variation in EMFs obtained with cobalt electrodes during replicate measurements were required for P estimations comparable to those obtained with standard laboratory instruments.

• Culinary science and hospitality research
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• v.22 no.7
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• pp.11-21
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• 2016

The purpose of this study was to analyse the impact of food tourism experience types on purchase intention and further to verify the influence of purchase intention on visitor satisfaction. For these purposes, a survey was given to 233 participants at the $15^{th}$ Daegu Food Tour Expo held from $9{\sim}12^{th}$ June 2016. The studay also carried out analysis of frequency, reliability, and correlation employing SPSS V. 20.0. The findings of the study are as follows. 1) Among 4 factors of food tourism experience types, gourmet, knowledge and healing pursuit groups havd a positive influence on purchase intention, although relation pursuit group had no significant influence on purchase intention. 2) It was discovered that purchase intention had a positive influence on visitor satisfaction. The results shown that food tourists have basic experience of gourmet with paying attention on knowledge and healing, as well. But food tourist has no significant influence on the group of relation pursuit. Accordingly, in order to encourage food tourists to re-visit, it is necessary to continuously develop and improve tour programs with well organized tour destinations and social system.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.6A
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• pp.355-367
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• 2012

Many of old aqueduct bridges located in rural areas are in need of repair and redesign. They still occupy some portion of countryside landscaping. However, most of them were designed to fulfill their basic functions of carrying waters, which has not contributed to the landscape positively. Moreover, it is not rational to treat each design case of aqueduct bridges individually because they are relatively small in size and arranged continuously over a long path. Therefore, it is better to provide a design guideline to repair or to redesign old aqueduct bridges as a whole considering both structural safety and landscape. The main objective is to develop a framework to repair and redesign of old aqueduct bridges for safety improvement and better landscape. Specifically this paper will address the development of possible design alternatives for repair and redesign The development of design alternatives for redesign will follow general principle of bridge aesthetics and be represented according to structural system, flume shape, pier height, pier shape in terms of design parameters while minor repair includes paintings and other ornamentations. And the developed design alternatives will be reviewed with its landscape as a background to check the visual compatibility within the community context. It is expected that the proposed guideline will be utilized to develop a maintenance plan to revitalize old aqueduct bridges to improve overall landscape of rural areas.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.256-260
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• 2012

This study was carried out to improve the fruit color through ABA treatment and to determine the optimum harvest time for producing high quality fruits in 'Hongisul' grapes. Spraying of $1000 mg{\cdot}L^{-1}$ exogenous ABA at early verasion (70 days after full bloom, DAFB) brought increase of endogenous ABA and soluble solid contents and enhanced fruit coloration. So, it was possible to harvest ABA treated fruits from 85 days after full bloom (15 days after treatment) by accelerated anthocyanin content which increased continuously until 110 DAFB. An increase of soluble solid and decrease of total acidity appeared steadily with the onset of berry ripening. After 100 DAFB, soluble solid content and total acidity did not change significantly, but the berry firmness was suddenly decreased. Consequently, it was suggested that ABA treated fruits need to be harvested at about 100 DAFB because of their short period of marketing by over ripening. On the contrary, harvesting of untreated fruit was totally impossible at 85 DAFB because of their poor berry coloration. But it was possible to harvest them at 100 DAFB based on the soluble solid/acidity ratio, whereas the berry coloration was progressed poorly. On the other hand, the fruits harvested at 110 DAFB showed acceptable berry coloration but their berry firmness was dropped significantly coincide with overripening. Therefore, it was needed to develop an altered production system for improving coloration at around 100 DAFB in 'Hongisul' grapes.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.40 no.6
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• pp.58-67
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• 2003

Many adult diseases(cerebral apoplexy, athymiait, etc.) result from hypertension, blood circulation disturbance and increment of HBP. In early diagnosis of these diseases, MRI, X-ray and PET have been used rather aim for treatment than prevention of a disease. Since, cerebral apoplexy and athymiait have been caused to the regular/irregular persons, it is very important to measure HBP which has connection with cerebral blood low state. HBP has more diagnosis elements than that of BP. So, we can diagnose accurate hypertension by measuring of HBP. But, existing sphygmomanometers and automatic BP monitors can not measure HBF, and can not execute complex function(measuring of BP/HBP, blood flow improvement). The purpose of this paper is to develop the system and algorithm which can measure BP/HBP for accurate diagnosis. Also, we extracted diagnosis factors by the correlativity analysis of BP/HBP. The maximum pressure of HBP corresponds to 62% that of BP, the minimum pressure of HBP corresponds to 46% that of BP. Therefore, we developed the multi function automatic blood pressure monitor which can measure BP/HBP and improve cerebral blood flow state.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.11
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• pp.1172-1182
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• 2014

In these days, many kinds of goods are more light and more integrated. As frequency range of mobile applications have increased to improve performance of antenna furthermore, EMI(ElectroMagnetic Interference) problem has frequently caused by disturbance of antenna in device which aggravates other circuit. This paper proposes a technique for the prediction of noise power to the transmission line from antenna located near the line. Although noise power transferred to transmission line is varied by source impedance of antenna and load impedance of transmission line basically, the power magnitude can be presented in a square form of S-parameter between antenna and transmission line due to small variation of transferred power. For this reason, we can use the index expressed the transferred power varied along geometrical shapes of transmission line. As a result, big difference is occurred along location of antenna especially the bended line. And this such experiment is correspond with simulation, these results have meaning physically considering electromagnetic field distribution in near and far field. HFSS of Ansys and CPW with ground is used in this paper.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.10
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• pp.1683-1690
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• 2020

Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including realtime polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.