• Reproductive and Developmental Biology
• /
• 제32권3호
• /
• pp.183-191
• /
• 2008

This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax-$\alpha$ and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권12호
• /
• pp.1816-1825
• /
• 2019

Objective: We tried to analyze allele-specific expression in the pig neocortex using bioinformatic analysis of high-throughput sequencing results from the parental genomes and offspring transcriptomes from reciprocal crosses between Korean Native and Landrace pigs. Methods: We carried out sequencing of parental genomes and offspring transcriptomes using next generation sequencing. We subsequently carried out genome scale identification of single nucleotide polymorphisms (SNPs) in two different ways using either individual genome mapping or joint genome mapping of the same breed parents that were used for the reciprocal crosses. Using parent-specific SNPs, allele-specifically expressed genes were analyzed. Results: Because of the low genome coverage (${\sim}4{\times}$) of the sequencing results, most SNPs were non-informative for parental lineage determination of the expressed alleles in the offspring and were thus excluded from our analysis. Consequently, 436 SNPs covering 336 genes were applicable to measure the imbalanced expression of paternal alleles in the offspring. By calculating the read ratios of parental alleles in the offspring, we identified seven genes showing allele-biased expression (p<0.05) including three previously reported and four newly identified genes in this study. Conclusion: The newly identified allele-specifically expressing genes in the neocortex of pigs should contribute to improving our knowledge on genomic imprinting in pigs. To our knowledge, this is the first study of allelic imbalance using high throughput analysis of both parental genomes and offspring transcriptomes of the reciprocal cross in outbred animals. Our study also showed the effect of the number of informative animals on the genome level investigation of allele-specific expression using RNA-seq analysis in livestock species.

• Nano
• /
• 제13권11호
• /
• pp.1850131.1-1850131.19
• /
• 2018

In this paper, an electrochemical sensor for epinephrine (EP), a neurotransmitter was developed by anchoring molecularly imprinted polymeric matrix (MIP) on the surface of gold-coated quartz crystal electrode of electrochemical quartz crystal microbalance (EQCM) using starch nanoparticles (Starch NP) - reduced graphene oxide (RGO) nanocomposite as polymeric format for the first time. Use of EP in therapeutic treatment requires proper dose and route of administration. Proper follow-up of neurological disorders and timely diagnosis of them has been found to depend on EP level. The MIP sensor was developed by electrodeposition of starch NP-RGO composite on EQCM electrode in presence of template EP. As the imprinted sites are located on the surface, high specific surface area enables good accessibility and high binding affinity to template molecule. Differential pulse voltammetry (DPV) and piezoelectrogravimmetry were used for monitoring binding/release, rebinding of template to imprinted cavities. MIP-coated EQCM electrode were characterized by contact angle measurements, AFM images, piezoelectric responses including viscoelasticity of imprinted films, and other voltammetric measurements including direct (DPV) and indirect (using a redox probe) measurements. Selectivity was assessed by imprinting factor (IF) as high as 3.26 (DPV) and 3.88 (EQCM). Sensor was rigorously checked for selectivity in presence of other structurally close analogues, real matrix (blood plasma), reproducibility, repeatability, etc. Under optimized conditions, the EQCM-MIP sensor showed linear dynamic ranges ($1-10{\mu}M$). The limit of detection 40 ppb (DPV) and 290 ppb (EQCM) was achieved without any cross reactivity and matrix effect indicating high sensitivity and selectivity for EP. Hence, an eco-friendly MIP-sensor with high sensitivity and good selectivity was fabricated which could be applied in "real" matrices in a facile manner.

• 한국기계가공학회지
• /
• 제14권4호
• /
• pp.1-7
• /
• 2015

In the present study, copper micromolds with a microhole array were precisely manufactured by a synchrotron LIGA process. Like in the traditional LIGA process, a deep X-ray lithography based on a synchrotron radiation was employed as the first manufacturing step. Due to the excellent optical performance of the synchrotron X-ray used, cylindrical micropillar arrays with high aspect ratio could be efficiently obtained. The fabricated microfeatures were then used as a master of the subsequent copper electroforming process, thereby resulting in copper micromolds with a microhole array. Thermoplastic hot embossing experiments with the copper micromolds were carried out for imprinting cylindrical microfeatures onto a polystyrene sheet. Through the hot embossing, the effect of embossing temperature and usefulness of the present manufacturing method could be verified.

• 한국표면공학회:학술대회논문집
• /
• 한국표면공학회 2012년도 춘계학술발표회 논문집
• /
• pp.153-153
• /
• 2012

자연계에는 다양한 생물체들의 표면 구조가 특수한 기능을 갖는 형태로 되어 있다. 이와 같이 특수한 기능을 갖는 생물체들의 표면 구조는 일반적으로 화학적 조성과 표면의 더불어 나노와 마이크로 구조가 혼합되어 있는, 이른바 hierarchical 구조를 보인다. 그 중에서도 표면 초소수성 특성을 보이는 연잎의 표면과 같은 hierarchical 구조는 self-cleaning effect 등의 기능성 표면 제작에 활용이 가능하여 이를 모사하기 위한 연구가 활발히 진행중에 있다. 이에 본 연구에서는 연잎과 같은 초소수성을 띄는 ZnO nano-in-micro hierarchical 구조를 저온 공정을 통하여 다양한 기판에 제작하였다. 이를 위하여 ZnO 나노 입자 분산 레진을 제작하였고 UV imprinting 과 수열합성법을 통하여 마이크로 패턴 상부에 ZnO 나노 입자가 형성된 ZnO nano-in-micro hierarchical 구조를 형성하였다. 제작된 ZnO hierarchical 구조의 젖음 특성은 표면 접촉각이 $160^{\circ}C$이상인 초소수성을 보였으며, 제작 공정에는 고온의 열처리가 수반되지 않아 PET film 등 다양한 기판에 ZnO hierarchical 구조를 제작할 수 있었다.

• Journal of Animal Science and Technology
• /
• 제45권4호
• /
• pp.649-658
• /
• 2003

오리농법에 있어서 논 방사 직후의 새끼오리는 물을 포함한 논 환경에 충분히 적응하지 못하고 폐사하거나 사람에 대한 도피행동에 의해 벼를 밟아 넘어뜨리는 등의 피해가 발생하고 있다. 또한 방사종료 후 오리포획에 있어서도 많은 시간과 노력이 소요되고 있다. 본 시험에서는 이와 같은 문제를 오리 육추시 특정의 사육관리를 통한 행동학적 측면에서 해결하고자, 논 방사할 오리에 대해 각인학습과 길들이기 등 온순한 관리를 행한 순치구, 거칠고 난폭하게 관리하면서 공포심을 부여한 조폭구 및 각인학습이나 길들이기, 공포심 등 사람과의 접촉경험이 거의 없는 대조구의 3 처리구로 구분하고, 오리의 행동유형, 성장 및 벼 수량성에 대하여 검토하였다. 오리의 행동유형에 있어서 논 방사직후의 채식과 이동행동은 조폭구 및 대조구에 비해 순치구에서 가장 많았고, 방사 2주 후의 채식과 이동행동에서는 순치구 및 조폭구보다 대조구에서 증가하는 경향을 나타내었다. 사람에 대한 도피반응거리에 있어서는 순치구에서 전혀 도피반응을 보이지 않았고, 조폭구보다 대조구에서 유의적으로 길게 나타났다. 오리 포획시간은 순치구에서 가장 짧았고, 다음으로 대조구, 조폭구의 순으로 나타났으며, 오리 순치방법에 따른 증체량에서는 처리구간에 큰 차이가 없었다. 벼 결주발생율은 대조구에 비해 순치구에서 유의적으로 적었고, 수량성에 대해서는 처리구간에 유의적인 차이는 인정되지 않았다. 이상의 시험결과를 종합해 볼 때, 오리 육추시 및 논 방사시의 각인학습과 온순한 관리는 벼의 결주 발생을 경감시키고, 오리가 사람에게 쉽게 순화되어 포획하는데 소요되는 시간과 노력이 현저하게 감소되는 것을 알 수 있었다. 그러나 논에서 오리의 노동행동과 벼 수량성에는 큰 영향을 미치지 않는 것으로 나타났다.

• 한국재료학회:학술대회논문집
• /
• 한국재료학회 2003년도 추계학술발표강연 및 논문개요집
• /
• pp.71-71
• /
• 2003

Hot embossing has been widely accepted as an alternative to photolithography in generating patterns on polymeric substrates. The optimization of embossing process should be accomplished based on polymer substrate materials. In this paper, the effect of polymer substrates on nano scale hot embossing process was studied. Silicon molds with nano size patterns were fabricated by e-beam direct writing. Molds were coated with self-assembled monolayer (SAM) of (1, 1, 2.2H -perfluorooctyl)-trichlorosilane to reduce the stiction between mold and substrates. For an embossing, pressure of 55, 75 bur, embossing time of 5 min and temperature of above transition temperature were peformed. Polymethylmethacrylates (PMMA) with different molecular weights of 450,000 and 950,000, MR-I 8010 polymer (Micro Resist Technology) and polyaliphatic imide copolymer were applied for hot embossing process development in nano size. These polymers were spun coated on the Si wafer with the thickness between 150 and 200 nm. The nano size patterns obtained after hot embossing were observed and compared based on the polymer properties by scanning electron microscopy (SEM). The imprinting uniformity dependent on the Pattern density and size was investigated. Four polymers have been evaluated for the nanoimprint By optimizing the process parameters, the four polymers lead to uniform imprint and good pattern profiles. A reduction in the friction for smooth surfaces during demoulding is possible by polymer selection.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
• /
• pp.252-252
• /
• 2012

It is widely accepted that short exciton diffusion lengths of organic semiconductors with respect to the film thickness limit the charge (hole and electron) separation before excitons recombination in organic photovoltaic (OPV) cells. Therefore the efficient absorption of incident light within the thin active organic layer is of great importance to improve the power conversion efficiency (PCE) of the cells. In this work, we fabricated 2-dimensionally (2D) nano-patterned poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOST:PSS) layers using capillary phenomenon and nano-imprinting technology at the scale of several hundred nanometers. This 2D nano-patterned PEDOT:PSS layer exerted photonic crystal effect such as redirection of light paths and variation of light intensity at specified wavelengths. It is also expected that the consequently alternated light pass lengths and intensities change the absorption properties of zinc phthalocyanine (ZnPc) thin films grown on top of the nano-patterned PEDOT:PSS layer. The influence of conductivity and thickness of the PEDOT:PSS layer on the absorption properties of ZnPc thin films were also investigated.

• 한국실내디자인학회논문집
• /
• 제17권3호
• /
• pp.43-50
• /
• 2008

Commercial space, the types of which have been increasingly various and changing rapidly, has been generating new marketing concepts for space. Especially as the environmentally friendly lifestyle spreads around, the component of natural landscape such as plant, stone, or water has been used as an important part in space design, freshly imprinting the brand image beyond the idea of simple interior property. By combining commercial space design with 'the component of the natural landscape', we can improve the brand image, create the newness in the space, lead customers to stay longer in the space, and reinforce the decorative effect. The parts of the commercial space to which we can apply the element of natural landscape are facades, walls, floors, and show windows. Various examples of real application are found according to the areas of business and goods displayed. This thesis attempts to maximize the effect of commercial space by examining and analyzing various instances of space and provide the ways of applying the space that contains an aesthetic value. For the research, 60 articles, theses, reports that have the keyword related to interior landscape and marketing strategy in commercial space were used as references. From the references, 70 cases were selected and analyzed to find landscape application patterns. Also, 4 store cases that landscape application have been the key to their success were selected for the survey. In doing this, I presented the readers with the packaging technique which improves brand image, the effect of stage direction which helps sensitive communication with users, the application as interior structure and the effect of an object that is useful to aesthetic effect in the commercial space. Finally, I endeavored to provide possible problems to be produced when applying the natural element in the commercial space and matters to be attended to in the management.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권12호
• /
• pp.1680-1688
• /
• 2013

Many different approaches have been developed to improve the efficiency of animal cloning by somatic cell nuclear transfer (SCNT), one of which is to modify histone acetylation levels using histone deacetylase inhibitors (HDACi) such as trichostatin A (TSA). In the present study, we examined the effect of TSA on in vitro development of porcine embryos derived from SCNT. We found that TSA treatment (50 nM) for 24 h following oocyte activation improved blastocyst formation rates (to 22.0%) compared with 8.9% in the non-treatment group and total cell number of the blastocysts for determining embryo quality also increased significantly ($88.9{\rightarrow}114.4$). Changes in histone acetylation levels as a result of TSA treatment were examined using indirect immunofluorescence and confocal microscopy scanning. Results showed that the histone acetylation level in TSA-treated embryos was higher than that in controls at both acetylated histone H3 lysine 9 (AcH3K9) and acetylated histone H4 lysine 12 (AcH4K12). Next, we compared the expression patterns of seven genes (OCT4, ID1; the pluripotent genes, H19, NNAT, PEG1; the imprinting genes, cytokeratin 8 and 18; the trophoblast marker genes). The SCNT blastocysts both with and without TSA treatment showed lower levels of OCT4, ID1, cytokeratin 8 and 18 than those of the in vivo blastocysts. In the case of the imprinting genes H19 and NNAT, except PEG1, the SCNT blastocysts both with and without TSA treatment showed higher levels than those of the in vivo blastocysts. Although the gene expression patterns between cloned blastocysts and their in vivo counterparts were different regardless of TSA treatment, it appears that several genes in NT blastocysts after TSA treatment showed a slight tendency toward expression patterns of in vivo blastocysts. Our results suggest that TSA treatment may improve preimplantation porcine embryo development following SCNT.