• Journal of Microbiology and Biotechnology
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• 제29권4호
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• pp.548-557
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• 2019

Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3~89.5% accuracy) at species level identification (EDT, 70.3~78.9% accuracy; DT, less than 46.3~63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.

• Korean Journal of Radiology
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• 제19권6호
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• pp.1053-1065
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• 2018

Objective: To evaluate the clinical impact of using registration software for ablative margin assessment on pre-radiofrequency ablation (RFA) magnetic resonance imaging (MRI) and post-RFA computed tomography (CT) compared with the conventional side-by-side MR-CT visual comparison. Materials and Methods: In this Institutional Review Board-approved prospective study, 68 patients with 88 hepatocellulcar carcinomas (HCCs) who had undergone pre-RFA MRI were enrolled. Informed consent was obtained from all patients. Pre-RFA MRI and post-RFA CT images were analyzed to evaluate the presence of a sufficient safety margin (${\geq}3mm$) in two separate sessions using either side-by-side visual comparison or non-rigid registration software. Patients with an insufficient ablative margin on either one or both methods underwent additional treatment depending on the technical feasibility and patient's condition. Then, ablative margins were re-assessed using both methods. Local tumor progression (LTP) rates were compared between the sufficient and insufficient margin groups in each method. Results: The two methods showed 14.8% (13/88) discordance in estimating sufficient ablative margins. On registration software-assisted inspection, patients with insufficient ablative margins showed a significantly higher 5-year LTP rate than those with sufficient ablative margins (66.7% vs. 27.0%, p = 0.004). However, classification by visual inspection alone did not reveal a significant difference in 5-year LTP between the two groups (28.6% vs. 30.5%, p = 0.79). Conclusion: Registration software provided better ablative margin assessment than did visual inspection in patients with HCCs who had undergone pre-RFA MRI and post-RFA CT for prediction of LTP after RFA and may provide more precise risk stratification of those who are treated with RFA.

• 대한토목학회논문집
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• 제38권6호
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• pp.879-885
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• 2018

고속도로 위빙구간은 진입과 진출차량에 의한 상충이 발생되는 지점으로 본선과의 영향을 최소화하기 위하여 집산로를 설치토록 하고 있다. 집산로의 설계속도는 50km/h이나 실제 운전자는 설계속도에 맞춰 적정한 감속이 이루어지지 않고 있다. 따라서 본 연구에서는 운전행태, 설계기준차량제원, 운전자의 시야각등을 고려하여 차량의 안전한 진출입을 위해 고어 노면표시 끝에서 합류지점까지 적정 이격거리를 검토하였으며 분석결과 합류가능지점에서 약 60m의 이격거리가 필요한 것으로 분석되었다. 향후 본 연구결과를 집산로가 설치된 위빙구간에 적용시 진출입 차량간 상충으로 인한 운전자의 안전성 증진에 기여할 것으로 판단되며 집산로가 설치된 고속도로 위빙구간의 노면표시 설치기준으로 재정립 될 것으로 기대된다.

• 수산경영론집
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• 제49권3호
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• pp.67-83
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• 2018

This study is aimed at finding policy directions for Korean fisheries and fishing villages by using Delphi method for fisheries experts. Fisheries experts have highly evaluated the achievements of fostering aquaculture industry, seafood export support measures, and natural disasters relief and recovery arrangements among the policies promoted as so far. And it was recognized that policies such as fishery resources management, creation and recovery of fishery resources, improvement hygiene and seafood safety, and provision young fishermen with training and capacity building will be important. Future megatrends, for example changes in food consumption pattern, climate change, and demographic structure changes are expected to have a significant impact on fisheries and fishing villages. The Delphi survey indicates that the most important policy objective is to secure a stable fisheries production. In other words, fisheries policy in the future should be aimed at suppling sustainable seafood for popular consumption. Finding strategies and action plans that can achieve this goal will be an important policy issue. In conclusion, it is necessary that a number of fundamental researches carry out in Korea, which can lead to finding out a multifunctionality of fisheries and fishing village. In addition, it is important to expand the scope of fisheries policy, which can consider not only the fisheries producers but also seafood consumer's and young fishermen perspectives. Furthermore, it recommends that fishery policy needs to include fishery related industry as well as application of 4th industrial revolution technology to fishery.

• 시스템엔지니어링학술지
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• 제15권1호
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• pp.1-8
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• 2019

Reverse engineering involves examining a system or component so as to comprehend its structure, functionality, and operation. Creation of a system model in reverse engineering can serve several purposes: test generation, change impact analysis, and the creation of a new or modified system. When attempting to reverse engineering a system, often the most readily accessible information is the system description, which does not readily lend itself to use in Model Based System Engineering (MBSE). Therefore, it is necessary to be able to transform this description into a diagram, which clearly depicts the behavior of the system as well as the interaction between components. This study demonstrates how sequence diagrams can be extracted from the systems description. Using MBSE software, the sequence diagrams for the Engineered Safety Features Component Control System (ESF-CCS) of the Nuclear Power Plant are created. Sequence diagrams are chosen because they are a means of representing the systems behavior and the interaction between components. In addition, from these diagrams, the system's functional requirements can be elicited. These diagrams then serve as the baseline of the reverse engineering process and multiple system views are subsequently be created from them, thus speeding up the development process. In addition, the use of MBSE ensures that any additional information obtained from auxiliary sources can then be input into the system model, ensuring data consistency.

• Wind and Structures
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• 제29권4호
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• pp.247-270
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• 2019

Wind-resistant design of existing cooling tower structures overlooks the impacts of rainfall. However, rainstorm will influence aerodynamic force on the tower surface directly. Under this circumstance, the structural response of the super-large cooling tower (SLCT) will become more complicated, and then the stability and safety of SLCT will receive significant impact. In this paper, surrounding wind fields of the world highest (210 m) cooling tower in Northwest China underthree typical wind velocities were simulated based on the wind-rain two-way coupling algorithm. Next, wind-rain coupling synchronous iteration calculations were conducted under 9 different wind speed-rainfall intensity combinations by adding the discrete phase model (DPM). On this basis, the influencing laws of different wind speed-rainfall intensity combinations on wind-driving rain, adhesive force of rain drops and rain pressure coefficients were discussed. The acting mechanisms of speed line, turbulence energy strength as well as running speed and trajectory of rain drops on structural surface in the wind-rain coupling field were disclosed. Moreover, the fitting formula of wind-rain coupling equivalent pressure coefficient of the cooling tower was proposed. A systematic contrast analysis on its 3D distribution pattern was carried out. Finally, coupling model of SLCT under different working conditions was constructed by combining the finite element method. Structural response, buckling stability and local stability of SLCT under different wind velocities and wind speed-rainfall intensity combinations were compared and analyzed. Major research conclusions can provide references to determine loads of similar SLCT accurately under extremely complicated working conditions.

• Advances in Energy Research
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• 제6권1호
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• pp.75-90
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• 2019

Anthropogenic greenhouse gas emissions are rising rapidly despite efforts to curb release of such gases. One long term potential solution to offset these destructive emissions is the capture and storage of carbon dioxide. Partially depleted hydrocarbon reservoirs are attractive targets for permanent carbon dioxide disposal due to proven storage capacity and seal integrity, existing infrastructure. Optimum well completion design in depleted reservoirs requires understanding of prominent geomechanics issues with regard to rock-fluid interaction effects. Geomechanics plays a crucial role in the selection, design and operation of a storage facility and can improve the engineering performance, maintain safety and minimize environmental impact. In this paper, an integrated geomechanics workflow to evaluate reservoir caprock integrity is presented. This method integrates a reservoir simulation that typically computes variation in the reservoir pressure and temperature with geomechanical simulation which calculates variation in stresses. Coupling between these simulation modules is performed iteratively which in each simulation cycle, time dependent reservoir pressure and temperature obtained from three dimensional compositional reservoir models in ECLIPSE were transferred into finite element reservoir geomechanical models in ABAQUS and new porosity and permeability are obtained using volumetric strains for the next analysis step. Finally, efficiency of this approach is demonstrated through a case study of oil production and subsequent carbon storage in an oil reservoir. The methodology and overall workflow presented in this paper are expected to assist engineers with geomechanical assessments for reservoir optimum production and gas injection design for both natural gas and carbon dioxide storage in depleted reservoirs.

• Advances in Computational Design
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• 제4권2호
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• pp.119-139
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• 2019

Planning and management building projects should tackle the coordination of works and the management of limited spaces, traffic and supplies. Activities cannot be performed without the resources available and resources cannot be used beyond the capacity of workplaces. Otherwise, workspace congestion will negatively affect the flow of works. Better on-site management allows for substantial productivity improvements and cost savings. The procurement system should be able to manage a wider variety of materials and products of the required quality in order to have less stock, in less time, using less space, with less investment and avoiding multiple storage stations. The objective of this paper is to demonstrate the advantages of using the Chronographic modeling, by combining spatiotemporal technical scheduling with the 4D simulations, the Last Planner System and the Takt-time when modeling the construction of building projects. This paper work toward the aforementioned goal by examining the impact that material flow has on site occupancy. The proposed spatiotemporal model promotes efficient site use, defines optimal site-occupancy and workforce-rotation rates, minimizes intermediate stocks, and ensures a suitable procurement process. This paper study the material flow on the site and consider horizontal and vertical paths, traffic flows and appropriate means of transportation to ensure fluidity and safety. This paper contributes to the existing body of knowledge by linking execution and supply to the spatial and temporal aspects. The methodology compare the performance and procurement processes for the proposed Chronographic model with the Gantt-Precedence diagram. Two examples are presented to demonstrate the benefits of the proposed model and to validate the related concepts. This validation is designed to test the model's graphical ability to simulate construction and procurement.

• 한국식품과학회지
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• 제53권2호
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• pp.165-173
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• 2021

본 연구에서는 UPM에 의해 유발되는 NCI-H441 세포 내 ROS 생성과 그에 따른 tight junction 단백질(N-cadherin, fibronectin, occluding, ZO-1, 및 claudin-4 등)의 발현 억제를 확인하였고 ECL의 전처리로 인하여 해당 ROS 생성 및 tight junction 단백질 발현 억제 현상이 완화되는 보호효과를 확인하였다. 그 결과, 폐 세포에서 UPM에 의해 유발된 산화 스트레스 및 tight junction 단백질의 발현 감소가 ECL을 선 처리함으로써 억제되어 결과적으로 산화 스트레스와 같은 미세먼지에 의한 폐 손상으로부터 보호 효과를 나타내고 tight junction 수준의 유지를 확인하였다. 이러한 결과를 통해 ECL 추출물은 폐와 기관지의 보호효과를 나타내며 폐 관련 질병의 예방효과가 있는 기능성 천연 물질로써 활용이 가능할 것으로 기대된다.

• International Journal of Computer Science & Network Security
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• 제21권9호
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• pp.118-124
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• 2021

The article examines the formation of a competitive paradigm of economic security of industrial enterprises in the formation of a circular economy. The basic laws of industrial enterprises are formed, which determined the characteristics of competitive positions and threats. The basic competitive concepts and their application at maintenance of economic safety of the industrial enterprises in the conditions of formation of circular economy are described. Thus, the technological approach to the formation of a competitive paradigm is based on production technologies, opportunities for technological modernization and development of infrastructure and intellectual competencies. The institutional concept reveals the regulatory mechanisms for ensuring competitiveness through the protectionism of national industrial enterprises, standardization and regulation of market imbalances. The innovation-investment approach within the competitive paradigm is also manifested in the creation of competitive advantages due to the presence of active innovative developments and their commercialization, knowledge and competencies of staff, capitalization of intelligence and communications, constant updating of infrastructure and technologies. Collectively, innovation and investment effects on the level of economic security allow industrial enterprises to ensure resilience to increasing competition, the emergence of new market challenges in the formation of a circular economy. A strategic approach to the application of a competitive paradigm to ensure the economic security of industrial enterprises allows you to justify the prospects for development and design behavioral models to predict and assess potential threats. The concept of system management is based on the complexity of threat analysis, the integrity of the economic security system, system-forming functions and patterns of implementation of industrial development tasks in the formation of a circular economy. The application of the described concepts is formalized by the authors through the definition of the basic patterns, directions and characteristics of their impact on the elements of the security system of industrial enterprises in the formation of a circular economy.