• Title/Summary/Keyword: Immature rice

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DNA Delivery into Embryogenic Cells of Zoysiagrass(Zoysia japonica Steud.) and Rice(Oryza sativa L.) by Electroporation (Electroporation을 이용한 잔디(Zoysia japonica Steud.) 및 벼(Oryza sativa L.) 배발생세포로의 DNA 도입)

  • 박건환;최준수;윤충호;안병준
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.5
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    • pp.309-314
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    • 1994
  • To develop simple and efficient transformation methods of monocotyledonous plane, electroporation-mediated delivery of DNA into intact embryogenic cell clumps was investigated in zoysiagrass and rice. Calli of zoysiagrass, induced from 3-week-old immature embryos, were suspension-cultured in MS basic medium supplemented with 1.0 mg/t 2.4-D and used for elechuporation. Calli, derived from immature inflorescences of 20 mm lenth of rice, were also suspension-cultured on N6 basic medium supplemented with 1.0 mg/L 2.4-D. Suspension-cultured embryogenic cell clumps were electroporated in liqid MS medium added with a Plasmid DNA (30 $\mu\textrm{m}$/ml), pGA1074, encoding ${\beta}$-glucuronidiase (GUS). DNA delivery into the cells through cell walls and cell membrane was confirmed by the transient expression of the GUS gene. Cell clumps of zoysiagrass and rice, electroporated with 400 volt at 800 pF capacitance, expressed GUS gene activity at a mean frequency of 25 units (one unit = one clony of blue cells) per 200 ${\mu}\ell$ of packed cell volume. Untreated cells and healed non-embryogenic cells did not exhibit GUS activity These results indicate that electroporation-mediated transformation can use intact embryogenic cells (thus avoiding the use protoplasts) in zoysiagrass and rice.

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Weed Occurrence and Rice Yield as Affected by Environment Friendly Farming Methods (친환경 농법에 따른 논 잡초발생 차이와 벼 수량에 끼치는 영향)

  • Cho, Kwang-Min;Lee, Sang-Bok;Kim, Sun;An, Xue-Hua;Chun, Jae-Chul
    • Korean Journal of Weed Science
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    • v.31 no.3
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    • pp.279-288
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    • 2011
  • To suggest the weed management technique for environment friendly rice cultivation, we investigated occurrence patterns of weeds, the actual condition of weed management, and rice yield at the environment friendly agricultural complex located in Honam and Chungnam regions. The practical performance of weed management was relatively satisfactory in decreasing order of agricultural technique with golden-apple-snail (GAS) > agricultural technique with duck (Duck) > agricultural technique with rice bran (RB) > agricultural technique with soft-shelled turtle (ST). In the rice fields employed by agricultural technique with GAS, the dominant weeds were Echinochloa crus-galli, Ludwigia prostrata, Monochoria vaginalis, Sagittaria trifolia, and Aneilema keisak. However, E. crus-galli, M. vaginalis, L. prostrata, Aeschynomene indica and Bidens frondosa were found as dominant weeds at the fields using the Duck and E. crus-galli, M. vaginalis, L. prostrata, Polyganum hydropiper and Eleocharis kuroguwai at the fields using RB. In comparison of rice yield ($5.2\;MT\;ha^{-1}$) obtained from the conventional cultivation using herbicides, about 93% was reached by Duck, about 91% by GAS, about 92% by RB, and about 78% by ST. When rice qualities obtained from environment friendly rice cultivation were compared with those from the conventional cultivation, the producing rates of perfect kernel, immature kernel, immature opaque kernel, cracked rice, and damaged kernel were lower in the former cultivation, whereas contents of protein, amylose, and fatty acid were similar in the two cultivation methods. The problems found in the environment friendly agriculture were poor plowing and harrowing, carless irrigation management, and geological poor condition as cultivation area with cold water. These have caused severe infestation of weeds, frequent incident of disease and insect pest, and rice lodging. This resulted in reduction of rice yield as high as about 32 to 79% as compared with the conventional cultivation using herbicides.

Brassinosteroid Substances in Immature Zea mays Seeds (옥수수 종실의 Brassinosteroid 활성물질 탐색)

  • 박근형;김선재현규환
    • KSBB Journal
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    • v.8 no.3
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    • pp.300-305
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    • 1993
  • In order to explore the brassinosteroid-active components in Zea mays seeds, the methanol extract was purified by the sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as castasterone and teasterone by HPLC. The content of brassinosteroid in Zea mays seeds as converted into brassinolide was 3-8ng/g fresh weight.

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Introduction of Thermotolerant Gene into Rice Plant by Agrobacterium Mediated Transformation (Agrobacterium을 이용한 내열성 유전자의 벼로의 형질전환 및 발현)

  • Lee, Byung-Hyun;Lee, Hyo-Shin;Won, Sung-Hye;Jo, Jin-Ki
    • Current Research on Agriculture and Life Sciences
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    • v.17
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    • pp.39-43
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    • 1999
  • To investigate the function of chloroplast-localized small HSP in rice, the cDNA, Oshsp21, was introduced into rice plants via Agrobacterium-mediated gene transfer system. Calli induced from rice immature embryos were co-cultivated with a A. tumafaciens EHA101 that contained a plasmid, pIHSP21. The efficiency of plant regeneration from the calli co-cultivated with the Agrobacterium was about 30%. PCR and Southern blot analyses using genomic DNA revealed that gene for the chloroplast small HSP was introduced into the genome of rice. Expression of transgene was investigated by northern blot analysis. Results indicate that the transgene, Oshsp21, was constitutively expressed at normal growth temperature.

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Plant Regeneration from Cryopreserved Embryogenic Cell Suspension Cultures of Korean Rice (Oryza sativa L.) Cultivars (한국 벼 품종 배발생 현탁배양 세포의 초저온 보존과 식물체 재분화)

  • 김석원;정원중;민성란;배경숙;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.2
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    • pp.115-120
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    • 1995
  • A method for cryopreservation of suspension cultured embryogenic cells derived from immature zygotic embryos of rice (Korean cultivars, Donggin-byeo and Taebaeg-byeo) was developed. The highest cell regrowth after storage in liquid nitrogen was obtained when Donggin-byeo cells were cryoprotected with a mixture of 2 M DMSO and 0.4 M sucrose and Taebaeg-byeo cells with a mixture of 0.64 M DMSO and 0.4 M sucrose at frequencies of 88% and 90%, respectively, Pretreatment in a high osmotic medium was not necessary. Upon transfer to $N_{6}$ medium suplemented with lmg/L NAA and 5 mg/L kinetin, the regenerated calli gave rise to numerous somatic embryos which subsequently underwent development into plantlets. Among approximately 100 plantlets, 25% of them were albinos.s.

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Trend and Further Research of Rice Quality Evaluation (쌀의 품질평가 현황과 금후 연구방향)

  • Son, Jong-Rok;Kim, Jae-Hyun;Lee, Jung-Il;Youn, Young-Hwan;Kim, Jae-Kyu;Hwang, Hung-Goo;Moon, Hun-Pal
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47
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    • pp.33-54
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    • 2002
  • Rice quality is much dependent on the pre-and post harvest management. There are many parameters which influence rice or cooked rice qualitys such as cultivars, climate, soil, harvest time, drying, milling, storage, safety, nutritive value, taste, marketing, eating, cooking conditions, and each nations' food culture. Thus, vice evaluation might not be carried out by only some parameters. Physicochemical evaluation of rice deals with amy-lose content, gelatinizing property, and its relation with taste. The amylose content of good vice in Korea is defined at 17 to 20%. Other parameters considered are as follows; ratio of protein body-1 per total protein amount in relation to taste, and oleic/linoleic acid ratio in relation to storage safety. The rice higher Mg/K ratio is considered as high quality. The optimum value is over 1.5 to 1.6. It was reported that the contents of oligosaccharide, glutamic acid or its derivatives and its proportionalities have high corelation with the taste of rice. Major aromatic compounds in rice have been known as hexanal, acetone, pentanal, butanal, octanal, and heptanal. Recently, it was found that muco-polysaccharides are solubilized during cooking. Cooked rice surface is coated by the muco-polysaccharide. The muco-polysaccharide aye contributing to the consistency and collecting free amino acids and vitamins. Thus, these parameters might be regarded as important items for quality and taste evaluation of rice. Ingredients of rice related with the taste are not confined to the total rice grain. In the internal kernel, starch is main component but nitrogen and mineral compounds are localized at the external kernel. The ingredients related with taste are contained in 91 to 86% part of the outside kernel. For safety that is considered an important evaluation item of rice quality, each residual tolerance limit for agricultural chemicals must be adopted in our country. During drying, rice quality can decline by the reasons of high drying temperature, overdrying, and rapid drying. These result in cracked grain or decolored kernel. Intrinsic enzymes react partially during the rice storage. Because of these enzymes, starch, lipid, or protein can be slowly degraded, resulting in the decline of appearance quality, occurrence of aging aroma, and increased hardness of cooked rice. Milling conditions concerned with quality are paddy quality, milling method, and milling machines. To produce high quality rice, head rice must contain over three fourths of the normal rice kernels, and broken, damaged, colored, and immature kernels must be eliminated. In addition to milling equipment, color sorter and length grader must be installed for the production of such rice. Head rice was examined using the 45 brand rices circulating in Korea, Japan, America, Australia, and China. It was found that the head rice rate of brand rice in our country was approximately 57.4% and 80-86% in foreign countries. In order to develop a rice quality evaluation system, evaluation of technics must be further developed : more detailed measure of qualities, search for taste-related components, creation and grade classification of quality evaluation factors at each management stage of treatment after harvest, evaluation of rice as food material as well as for rice cooking, and method development for simple evaluation and establishment of equation for palatability. On policy concerns, the following must be conducted : development of price discrimination in conformity to rice cultivar and grade under the basis of quality evaluation method, fixation of head rice branding, and introduction of low temperature circulation.

Occurrence and Population Dynamics of Spiders in Transplanting Rice Fields under Different Levels of Pest Management (해충관리 수준이 다른 이앙재배 논에서의 거미의 발생과 개체군 동태)

  • Park, Hong-Hyun;Lee, Joon-Ho;Kim, Seung-Tae
    • The Korean Journal of Ecology
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    • v.28 no.5
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    • pp.287-293
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    • 2005
  • This study was conducted to evaluate the effect of the conventional pesticide application system on the rice spider community based on the quantitative investigation of arthropod community in the rice fields in Saran area, Hwasung-si, Kyeonggi-do from 1996 to 1998. Spiders were the highest dominant group comprising over 90% of all natural enemies in their density, and showed very stable yearly occurrence. In these fiekds the mean density of spiders was about 10 individuals/$0.25m^2$ during the rice growing season, and three families including Lycosidae, Tetragnathidae and Linyphiidae were very abundant. The colonization of spiders occurred in early season and they showed rapid density increase in mid-season. They maintained the highest density to the late season and their density decreased at harvest season. In the years of the low rice pest occurrence, the disturbance effect on spider community by pesticides were less. Two dominant species, Pirata subpiraticus and Pachygnatha clerki, had different population dynamics; Pirata subpiraticus showed the rapid density increase in the mid-July, and most of them remained immature stages, however, in the case of Pachygnaths clerki population, they rapidly increased in the late August and most of them remained adult stage during the late growing season.

Effect of parboiling on the physicochemical properties of immature barley kernels (Parboiling처리가 미숙보리곡립의 이화학적 특성에 미치는 영향)

  • Seog, Ho-Moon;Kim, Jong-Sang;Hong, Hee-Do;Kim, Sung-Soo;Kim, Kyung-Tack
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.456-462
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    • 1993
  • Parboiling, a popular technology used to protect rice from nutrient loss during milling and to increase the shelf-life of rice, was applied to barley kernels, and its effect on nutrient retention and chemical composition was evaluated. Before 36 days after ear emergence, barley kernels showed water content higher than 40%, and parboiling without presoaking resulted in at least 43% of gelatinization degree. This implies that soaking, an important step of parboiling, is dispensable for barley at milky stage. Parboiling did make little change in the appearance of the kernel after 31 days from ear emergence. Nonreducing sugars such as sucrose and raffinose remained unchanged while reducing sugars of barley was decreased by parboiling, with exception that maltose increased. Pearling led to decrease in crude protein, fat, fiber and minerals of barley sampled and parboiled on 36th day from ear emergence. Free sugars in the parboiled barley also was reduced with increasing pearling rate. Vitamin $B_1$ content of the parboiled barley was $260\;{\mu}g\;per\;100\;g$ as dry basis at 50% pearling rate, compared to $36\;{\mu}g$ for raw barley at same pearling rate. Thus parboiling appeared to be very effective in the retention of vitamin $B_1$ during pearling.

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Differences in Rice Quality and Physiochemical Component between Protox Inhibitor-Herbicide Resistant Transgenic Rice and Its Non-transgenic Counterpart (Protox 저해형 제초제 저항성 형질환벼와 비형질전환벼의 미질 및 이화학적 성분 차이)

  • Jung, Ha-Il;Yun, Young-Beom;Kwon, Oh-Do;Lee, Do-Jin;Back, Kyoung-Whan;Kuk, Yong-In
    • Korean Journal of Weed Science
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    • v.32 no.1
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    • pp.25-34
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    • 2012
  • Characteristics related to grain quality and physiochemical components such as mineral, total amino acid, free amino acid, and free sugar composition were investigated in Protox inhibitor resistanttransgenic rice (MX, PX, and AP37) and its nontransgenic counterpart (WT). Head rice, palatability, protein, and whiteness (except for MX and AP37) of milled transgenic rice were high or similar to those of the non-transgenic counterpart. Immature rice, unfilled grain, and cracked kernels (PX and AP37) of milled transgenic rice were lower than those of its non-transgenic counterpart. However, there were no significant differences in damaged grain between the transgenic rice lines and its counterpart. Potassium content in PX and copper contents in PX and AP37 were only low compared with their non-transgenic counterparts, but other mineral contents in transgenic rice lines were high or showed no significant differences compared with non-transgenic counterparts. Contents of most total amino acid composition in transgenic rice lines were high or similar to those in non-transgenic counterparts, but the content of isoleucine in AP37 was only low compared with its non-transgenic counterpart. On the other hand, free amino acid, leucine and tyrosine in PX and AP37, and total free amino acid in PX were low compared with their non-transgenic counterparts. However, the content of free amino acid in other kinds in transgenic rice lines were similar to those in their non-transgenic counterparts. Contents of sucrose in MX and PX were low compared with non-transgenic counterpars, but contents of fructose, glucose, and maltose in transgenic rice lines were high or similar compared with their non-transgenic counterparts. This results indicated that Protox genes had no negative affect on the nutritional composition of rice.