• Journal of Sensor Science and Technology
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• v.26 no.2
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• pp.107-113
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• 2017

Energy source of a piezo-electric harvester is vibration. Sources of vibration are machineries operated with high frequencies, constructions and people operated with low frequencies and etc. In this study, we tried to figure out power generation properties over vibrations upon angles of a piezo-cantilever for applying them to movements of the construction and/or people, which are vibration sources at low frequencies. A uni-morph cantilever with a $59mm{\times}29mm{\times}0.2mm$ piezo-electric element attached on a $71mm{\times}46mm{\times}0.25mm$ copperplate was used. A spring was attached to the lower side of the cantilever and a mass was attached on the opposite side. Also, a structure with a specific angle which is an angle in between the ground and the cantilever was prepared and then, connected to a spring or the cantilever. Then, this structure was divided into the A-type and B-type and excited in the direction of z- axis. After that, the angle between the ground and the cantilever was changed and excited by 1 to 10 Hz upon the existence of a spring and/or a mass to compare power generation properties.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.3
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• pp.217-223
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• 2018

Overfishing capacity has become a global issue due to over-exploitation of fisheries resources, which result from excessive fishing intensity since the 1980s. In the case of Korea, the fishing effort has been quantified and used as an quantified index of fishing intensity. Fisheries resources of coastal fisheries in the Korean waters of the East Sea tend to decrease productivity due to deterioration in the quality of ecosystem, which result from the excessive overfishing activities according to the development of fishing gear and engine performance of vessels. In order to manage sustainable and reasonable fisheries resources, it is important to understand the fluctuation of biomass and predict the future biomass. Therefore, in this study, we forecasted biomass in the Korean waters of the East Sea for the next two decades (2017~2036) according to the changes in fishing intensity using four fishing effort scenarios; $f_{current}$, $f_{PY}$, $0.5{\times}f_{current}$ and $1.5{\times}f_{current}$. For forecasting biomass in the Korean waters of the East Sea, parameters such as exploitable carrying capacity (ECC), intrinsic rate of natural increase (r) and catchability (q) estimated by maximum entropy (ME) model was utilized and logistic function was used. In addition, coefficient of variation (CV) by the Jackknife re-sampling method was used for estimation of coefficient of variation about exploitable carrying capacity ($CV_{ECC}$). As a result, future biomass can be fluctuated below the $B_{PY}$ level when the current level of fishing effort in 2016 maintains. The results of this study are expected to be utilized as useful data to suggest direction of establishment of fisheries resources management plan for sustainable use of fisheries resources in the future.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.4
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• pp.423-434
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• 2012

This study was carried out to develop for the emission factor of greenhouse gas (GHG) from medium and smallscaled incineration facility using RPF which is considering as a part of renewable energy in UNFCC. The actual concentration of the exhaust gas and the fuel composition of RPF were measured for the calculation of GHG emission factor in RPF incinerators, and were compared with the IPCC guideline. The $CO_2$ and $N_2O$ emission factors by the actual concentration of exhaust gas were $2.3575{\pm}1.0070tCO_2/tRPF$ and $0.0014{\pm}0.0014tN_2O/tRPF$ respectively. Also, $CO_2$ emission factor by the RPF composition was $2.7057{\pm}0.0540tCO_2/tRPF$. The GHG emission factor per energy by the actual concentration was $83.0867{\pm}26.0346tCO_2e/TJ$ which showed higher consistency with the GHG emission factor ($80.3967tCO_2e/TJ$) of waste plastic in the IPCC guideline (2006b). The $CO_2$ and $N_2O$ emission factor calculated in this study is considered as a meaningful data for GHG emission factor of RPF incineration facility because of not being developed in ROK.

• Progress in Superconductivity
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• v.11 no.1
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• pp.62-66
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• 2009

Introduction of proper impurity into $YBa_2Cu_3O_{7-x}$ (YBCO) thin films is an effective way to enhance its flux-pinning properties. We investigate effect of $Y_2O_3$ nanoparticles on the critical current density $J_c$ of the YBCO thin films. The $Y_2O_3$ nanoparticles were created perpendicular to the film surface (parallel with the c-axis) either between YBCO and substrate or on top of YBCO, YBCO/$Y_2O_3$/LAO or $Y_2O_3$/YBCO/STO, by pulsed laser deposition. The deposition temperature of the YBCO films were varied ($780^{\circ}C$ and $800^{\circ}C$) to modify surface morphology of the YBCO films. Surface morphology characterization revealed that the lower deposition temperature of $780^{\circ}C$ created nano-sized holes on the YBCO film surface which may behave as intrinsic pinning centers, while the higher deposition temperature produced much denser and smoother surface. $J_c$ values of the YBCO films with $Y_2O_3$ particles were either remained nearly the same or decreased for the samples in which YBCO is grown at $780^{\circ}C$. On the other hand, $J_c$ values were enhanced for the samples in which YBCO is grown at higher temperature of $800^{\circ}C$. The difference in the effect of $Y_2O_3$ can be explained by the fact that the higher deposition temperature of $800^{\circ}C$ reduces intrinsic pinning centers and $J_c$ is enhanced by introduction of artificial pinning centers in the form of $Y_2O_3$ nanoparticles.

• BMB Reports
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• v.28 no.3
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• pp.265-270
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• 1995

Acetolactate synthase (ALS, EC 4.1.3.18) is the first common enzyme in the biosynthesis of leucine, isoleucine, and valine. It is the target enzyme for several classes of herbicides, including the sulfonylureas, the imidazolinones, the triazolopyrimidines, the pyrimidyl-oxy-benzoates and the pyrimidyl-thio-benzens. The sulfonylurea-resistant ALS gene (SurB) from Nicotiana tabaccum [Lee et al. (1988) The EMBO J. 7, 1241-1248] was cloned into the bacterial expression plasmid pT7-7. The resulting recombinant plasmid pT7-ALS was used to transform an ALS-deficient Escherichia coli strain MF2000. MF2000 cells transformed with pT7-ALS grew in the absence of valine and isoleucine. ALS activities of 0.042 and 0.0002 ${\mu}mol/min/mg$ protein were observed in the crude extracts prepared from MF2000 cells transformed with plasmids pT7-ALS and pT7-7, respectively. In addition, the former crude extract containing mutant ALS was insensitive to inhibition by K11570, a new chemical class of herbicides. $IC_{50}$ values for K11570 were $0.13{\pm}0.01$ mM. For comparison, a plasmid pTATX containing the wild-type Arabidopsis thaliana ALS coding sequences was also expressed in MF2000. ALS activities of 0.037 ${\mu}mol/min/mg$ protein were observed, and the wild type ALS was sensitive to two different classes of herbicides, K11570 and ALLY, a sulfonylurea. $IC_{50}$ values for K11570 and ALLY were $0.63{\pm}0.07$ and $80{\pm}5.6$ nM, respectively. Thus, the results suggest that the sulfonylurea-resistant tobacco ALS was functionally expressed in the bacteria, and that K11570 herbicides bind to the regulatoty site of ALS enzymes.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.3C
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• pp.147-152
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• 2005

In this paper, we proposed a method for compressing the static video test patterns in high quality to test the picture quality in DTV. In our method, we use the fact that the generated bits and average quantization value have almost identical distribution characteristics per each GOP and we propose a new target bit allocation method suitable for compressing the static test pattern while the target bit allocation method in MPEG-2 TM5 is suitable for the moving picture. The proposed target bit allocation method is to maintain the high quality video continuously by using the normalized complexities which are updated or maintained by means of picture qualities at each GOP. Experiment result showed that the test pattern stream encoded by MPEG-2 software with the proposed algorithm had a stable bit rate and good video quality during the decoding process.

• International Journal of Industrial Entomology and Biomaterials
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• v.17 no.1
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• pp.131-135
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• 2008

A cDNA encoding a defensin-like peptide (Protaetiamycine) from the larvae of a beetle, Protaetia brevitarsis was cloned. The DNAs encoded the deduced propeptide of 79 amino acid residues with the predicted molecular weight of 8.4 kDa and PI of 8.24. Overall amino acid sequence of this protein has 39% similarity to that of Rhodnius prolixus defensin, 43% similarity to that of Acalolepta luxuriosa defensin, and 72% similarity to that of Oryctes rhinoceros defensin, suggesting that this gene is an insect defensin. In an attempt to apply the anti-bacterial peptide to the development of therapeutic agents, a 12-mer peptide amidated at its C-terminus, ACAAHCLAIGRG-$NH_2$ (Ala55-Lys66-$NH_2$, 12Pbn) was synthesized. This peptide showed some antifungal activity against Candida albicans. To increase antifungal activity, six 9-mer peptides were synthesized by modifying amino acid sequences of 12Pbn fragment. Among these peptides, 9Pbm3-9Pbm6 exhibited strong activity compared with Cecropin B and mellitin.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.696-703
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• 2014

Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-induced apoptosis, cytoskeletal disaggregation, cell detachment, and tight junction loss in cultured colonic epithelial cells. Serum samples were isolated from mice that survived a C. difficile infection following antibiotic treatment, and the antitoxin effects of these samples were investigated in toxin-A-exposed HT29 colonic epithelial cells and a toxin-A-induced animal model of gut inflammation. Unchallenged mice did not produce IgG against toxin A, whereas serum (antiserum) from C. difficile-challenged mice showed significant IgG responses against toxin A. Treatment with the antiserum markedly inhibited mucosal damage and inflammation in the toxin-A-treated mouse model. In contrast to control mouse serum, the antiserum also markedly inhibited toxin-A-induced DNA fragmentation, dephosphorylation of paxillin and Epo receptor (EpoR), deacetylation of tubulin, and upregulation of p21(WAF1/CIP1) and p53. Taken together, these results reveal that the generated antitoxin serum has biotherapeutic effects in preventing various C. difficile toxin-A-induced cellular toxicities.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.43-43
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• 2003

This study was conducted to produce transgenic pig harboring human tissue plasminogene activator (tPA) gene. Two different tPA genes containing bovine $\beta$-casein promoter and mouse uroplakin promoter were prepared for microinjection and confirmed the expression level of tPA protein from the CHO (Chinese hamster ovary) cell lines by gene transfection. Concentration of tPA expression from the six cell lines (all of CHO cells) were average 212.4 ng/ml. Reconstructed DNA to used the CHO cell were microinjected into the pronuclei of in vivo embryos The total of 2,307 zygotes were collected from 95 donors and 1,851 embryos were in 1-cell stage which were visualized the pronuclei for DNA microinjection. The concentration of linear DNA was 2.0 ng per microliter and injected into zygotes with two pronuclei on an inverted Nikon microscope equipped with narishige micromanipulator and modulation contrast optics. The 541 embryos injected with bovine $\beta$-casein promoter-tPA were transferred to 22 recipients. The 1,154 embryos injected with mouse uroplakin promoter-tPA were transferred to 51 recipients. Sixty nine offspring from 9 delivered sows were produced. We analysed the transgenes with PCR methods from 69 offsprings, but could not detect the PCR product from piglet tails DNA.

• International Journal of Safety
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• v.3 no.1
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• pp.6-9
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• 2004

This paper deals with the noise evaluation technique of a reciprocating air-compressor and its noise reduction. The reciprocating air-compressors are widely used in the small, medium sized industrial firms, and lots of their employees are affected and irritated by their noise in the workplace. Thus, noise control actions should be taken appropriately by considering the hearing loss due to the occupational noise exposure. Lead-wrapping techniques are employed to identify the contribution of principal noise sources which are generally known as motor, belts, suction/discharge valves, moving pistons, and flow-induced noise caused by edges or discontinuities along the flow path e.g. expansions, contractions, junctions and bends etc .. As a result, main noise sources of the air-compressor can be categorized by the suction/discharge noise, valve noise, and compressed-air tank noise. Based on the investigations, mufflers are designed to reduce both the suction/discharge noise and the compressed-air tank noise. Instead of the conventional valve plate, polyethylene resin is used as a new one for the reduction of valve impact noise. In addition, attempts are made to reduce the valve noise propagation to the cylinder head and the compressor tank by using the insulation casings. As a result of the countermeasure plans, it can be achieved that the noise reduction of the air-compress is up to 10dB.