• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
• /
• 2001.06a
• /
• pp.4105-4105
• /
• 2001

Near infrared spectroscopy (NIRS) was employed to qualify and quantify on survival, the injury rate and apoptosis of the human breast cancer cell line MCF-7 cells. MCF-7 cells were cultured in RPMI medium supplemented with 10% FCS in a 95% air and 5% CO2 atmosphere at 37$^{\circ}C$. For the viable cells preparation, cells were de-touched by 0.1% of trypsin treatment and washed with RPMI supplemented with 10% FCS medium by centrifugation at 1000 rpm for 3min. For the dead cells preparation, cells were de-touched by a cell scraper. The cells were counted by a hemacytometer, and the viability was estimated by the exclusion method with frypan blue dye. Each viable and dead cells were suspended in PBS (phosphate bufferred saline) or milk at the cell density desired. For the quantitative determination of cell death by measuring the LDH (lactate dehydrogenase) activity liberated from cells with cell membrane injuries, LDH-Cytotoxic Test Wako (Wako, Pure Pharmaceutical Co. Ltd., Japan) was used. We found that NIRS measurement of MCF-7 cells at the density range could evaluate and monitor the different characteristics of living cells and dead cells. The spectral analysis was performed in two wavelength ranges and with 1,4, 10 mm pathlength. Different spectral data pretreatment and chemometrics methods were used. We applied SIMCA classificator on spectral data of living and dead cells and obtained good accuracy when identifying each class. Bigger variation in the spectra of living cells with different concentrations was observed when compared to the same concentrations of dead cells. PLS was used to measure the number of cells in PBS. The best model for measurement of dead cells, as well as living cells, was developed when raw spectra in the 600-1098 nm region and 4 mm pathlength were used. Smoothing and second derivative spectral data pretreatment gave worst results. The analysis of PLS loading explained this result with the scatter effect found in the raw spectra and increased with the number of cells. Calibration for cell count in the 1100-2500 nm region showed to be very inaccurate.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.9
• /
• pp.1184-1191
• /
• 2011

We attempted to generate a linkage map using Illumina Porcine 60K SNP Beadchip genotypes of the $F_2$ offspring from Korean native pig (KNP) crossed with Yorkshire (YS) pig, and to identify quantitative trait loci (QTL) using the line-cross model. Among the genotype information of the 62,136 SNPs obtained from the high-density SNP analysis, 45,308 SNPs were used to select informative markers with allelic frequencies >0.7 between the KNP (n = 16) and YS (n = 8) F0 animals. Of the selected SNP markers, a final set of 500 SNPs with polymorphic information contents (PIC) values of >0.300 in the $F_2$ groups (n = 252) was used for detection of thirty meat quality-related QTL on chromosomes at the 5% significance level and 10 QTL at the 1% significance level. The QTL for crude protein were detected on SSC2, SSC3, SSC6, SSC9 and SSC12; for intramuscular fat and marbling on SSC2, SSC8, SSC12, SSC14 and SSC18; meat color measurements on SSC1, SSC3, SSC4, SSC5, SSC6, SSC10, SSC11, SSC12, SSC16 and SSC18; water content related measurements in pork were detected on SSC4, SSC6, SSC7, SSC10, SSC12 and SSC14. Additional QTL of pork quality traits such as texture, tenderness and pH were detected on SSC6, SSC12, SSC13 and SSC16. The most important chromosomal region of superior pork quality in KNP compared to YS was identified on SSC12. Our results demonstrated that a QTL linkage map of the $F_2$ design in the pig breed can be generated with a selected data set of high density SNP genotypes. The QTL regions detected in this study will provide useful information for identifying genetic factors related to better pork quality in KNP.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.48 no.5
• /
• pp.28-41
• /
• 2020

This study selects two linear parks representing each culture and reveals the differences between them using a visitor survey as small data and social media analytics as big data based on the three components of the model of landscape perception. The 606 in Chicago, U.S., and the Gyeongchun Line in Seoul, Korea, are representative parks built on railroads. A total of 505 surveys were collected from these parks. The responses were analyzed using descriptive statistics, principal component analysis, and linear regression. Also, more than 20,000 tweets which mentioned two linear parks respectively were collected. By using those tweets, the authors conducted the clustering analysis and draw the bigram network diagram for identifying and comparing the placeness of each park. The result suggests that more diverse design concept links to less diversity in behavior; that half of the park users use the park as a shortcut; and that same physical exercise provides different benefits depending on the park. Social media analysis showed the 606 is more closely related to the neighborhoods rather than the Gyeongchun Line Forest. The Gyeongchun Line Forest was a more event-related place than the 606.

• Journal of Navigation and Port Research
• /
• v.39 no.3
• /
• pp.199-207
• /
• 2015

In ports of Korea, the marine traffic flow is congested due to a large number of vessels coming in and going out. In order to improve the safety and efficiency of these vessels, South Korea is operating with a Vessel Traffic Service System, which is monitoring its waters for 24 hours. However despite these efforts of the VTS (Vessel Traffic Service) officers, collisions are occurring continuously, the risk situation is analyzed that occurs once in about 20 minutes, the risk may be greater. It investigated to reduce these accidents by providing a safety standard for collision danger in a timely manner. Thus, this study has developed a risk prediction module to predict risk in advance. This module can avoid collision risk to adjust the speed and course of ship using a risk evaluation model based on ship operator's risk perspective. Using this module, the ship operators and VTS officers can easily be identified risks in complex traffic situations, so they can take an appropriate action against danger in near future including course and speed change. To verify the effectiveness of this module, this paper predicted the risk of each encounter situation and confirmed to be capable of identifying a risk changes in specific course and speed changes at Busan coastal water.

• The Korean Journal of Applied Statistics
• /
• v.27 no.4
• /
• pp.523-541
• /
• 2014

We apply a Bayesian estimation procedure to the analysis of roll call voting records on 2,389 bills processed during the 18th Korean National Assembly. The analysis of roll calls yields useful tools for to combining the measurement of legislative preference with the models of legislative behavior. The current Bayesian procedure is extremely exible, applicable to any legislative setting, irrespective of the extremism of the legislator's voting history or the number of roll calls available for analysis. It can be applied to any legislative settings, providing a useful solution to many statistical problems inherent in the analysis of roll call voting records. We rst estimate the ideal points of all members of the 18th National Assembly and their condence intervals. Subsequently, using the estimated ideal points, we examine the factional disparity within each major party using the estimated ideal points. Our results clearly suggest that there exists a meaningful ideological spectrum within each party. We also show how the Bayesian procedure can easily be extended to accommodate theoretically interesting theoretical models of legislative behavior. More specically, we demonstrate how the estimated posterior probabilities can be used for identifying pivotal legislators.

• Journal of Korean Society of Transportation
• /
• v.30 no.4
• /
• pp.95-106
• /
• 2012

Driver's capability of identifying the change in freeway alignments and environments is one of important factors associated with traffic safety on freeways. In particular, driver's visibility and recognition capability are highly dependent on the altitude of the sun by sunset, sunrise, and nighttime. The purpose of this study is to identify the characteristics of geometric conditions affecting crash occurrences at sunset, sunrise, and nighttime. Poisson and negative binomial regressions were adopted to predict freeway crash frequency in this study. Freeway crash data during 2007~2010 were used for developing the crash frequency models. A set of variables representing the characteristics of geometric conditions were identified as significant ones affecting crash occurrences. The results of this study would be useful in deriving effective countermeasures for preventing traffic crashes that mainly occur at sunset, sunrise, and nighttime on freeways.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.34 no.3
• /
• pp.329-338
• /
• 2016

As the need of indoor spatial information has grown, many applications have been developed. Nevertheless, the major representations of indoor spatial information are on the 2D or 3D, recently, the service based on omni-directional image has increased. Current service based on omni-directional image is used just for viewer. To provide various applications which can serve the identifying the attribute of indoor space, query based services and so on, topological data which can define the spatial relationships between spaces is required. For developing diverse applications based on omni-directional image, this study proposes the method to generate IndoorGML data which is the international standard of indoor topological data model. The proposed method is consist of 3 step to generate IndoorGML data; 1) Analysis the core elements to adopt IndoorGML concept to image, 2) Propose the method to identify the element of ‘Space’ which is the core element of IndoorGML concept, 3) Define the connectivity of indoor spaces. The proposed method is implemented at the 6-floor of 21centurybuilding of the University of Seoul to generate IndoorGML data and the demo service is implemented based on the generated data. This study has the significance to propose a method to generate the indoor topological data for the indoor spatial information services based on the IndoorGML.

• Journal of KIISE:Software and Applications
• /
• v.35 no.9
• /
• pp.562-571
• /
• 2008

For the purpose of building domain ontology, this paper proposes a methodology for building core ontology first, and then enriching the core ontology with the concepts and relations in the domain thesaurus. First, the top-level concept taxonomy of the core ontology is built using domain dictionary and general domain thesaurus. Then, the concepts of the domain thesaurus are classified into top-level concepts in the core ontology, and relations between broader terms (BT) - narrower terms (NT) and related terms (RT) are classified into semantic relations defined for the core ontology. To classify concepts, a two-step approach is adopted, in which a frequency-based approach is complemented with a similarity-based approach. To classify relations, two techniques are applied: (i) for the case of insufficient training data, a rule-based module is for identifying isa relation out of non-isa ones; a pattern-based approach is for classifying non-taxonomic semantic relations from non-isa. (ii) For the case of sufficient training data, a maximum-entropy model is adopted in the feature-based classification, where k-NN approach is for noisy filtering of training data. A series of experiments show that performances of the proposed systems are quite promising and comparable to judgments by human experts.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.4 no.4
• /
• pp.277-286
• /
• 2002

Most of the problems in dealing with the tunnel construction are the uncertainties and complexities of the stress conditions and rock strengths in ahead of the tunnel excavation. The limitations on the investigation technology, inaccessibility of borehole test in mountain area and public hatred also restrict our knowledge on the geologic conditions on the mountainous tunneling area. Nevertheless an extensive and superior geophysical exploration data is possibly acquired deep within the mountain area, with up to the tunnel locations in the case of alternative design or turn-key base projects. An appealing claim in the use of artificial neural networks (ANN) is that they give a more trustworthy results on our data based on identifying relevant input variables such as a little geotechnical information and biological learning principles. In this study, error back-propagation algorithm that is one of the teaching techniques of ANN is applied to presupposition on Rock Mass Ratings (RMR) for unknown tunnel area. In order to verify the applicability of this model, a 4km railway tunnel's field data are verified and used as input parameters for the prediction of RMR, with the learned pattern by error back propagation logics. ANN is one of basic methods in solving the geotechnical uncertainties and helpful in solving the problems with data consistency, but needs some modification on the technical problems and we hope our study to be developed in the future design work.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.11
• /
• pp.770-777
• /
• 2017

Building information modeling (BIM) has been receiving attention as an integrated information model instead of CAD since the 2000s. BIM technology was first used in the architectural field and was later introduced to the civil engineering field. However, the government announced a plan for the application of BIM to 20% of all SOC projects from 2020, so the adoption of BIM technology is expected to accelerate. In order to successfully adopt BIM, a systematic structure should be supported for integrated design information and implementation technology. Also, it is important to establish the relationship between information systems because many complicated factors are intertwined in the construction industry. In this study, we propose a framework for constructing integrated information through identifying the information relations for introducing BIM in the civil engineering industry. We applied this framework to a bridge project to confirm its effectiveness. This study can be applied to the integrated management of the construction process and costs by introduction of a work breakdown structure (WBS) to BIM. In addition, this study is expected to contribute to the adoption of BIM in the civil engineering field through the proposal of information system standardization in this field.