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Analysis of Nodakenetin from Samultangs Fermented by Lactose Bactera Strains (유산균 발효에 의한 사물탕들부터 노다케네틴의 분리 및 함량분석)

  • Kim, Dong-Seon;Roh, Joo-Hwan;Cho, Chang-Won;Ma, Jin-Yeul
    • The Korea Journal of Herbology
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    • v.27 no.1
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    • pp.35-39
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    • 2012
  • Objectives : The purpose of this study was to investigate the changes in the contents of constituents in Samultang and its fermentations with 10 species of lactic acid bacteria. Methods : Ten strains of lactic acid bacteria, Lactobacillus casei, L. acidophilus, L. casei, L. plantarum, L. amylophilus, L. curvatus, L. delbruekil subsp. lactis, L. casei, B. breve, and B. thermophilum, were used for the fermentation of Samultang. The increased and decreased constituents were identified using HPLC/DAD and various liquid chromatographic techniques, and the structure was elucidated using NMR and MS. These compounds were quantitatively analyzed using an HPLC/DAD system. Results : A remarkably increased component was identified to be nodakenetin and a decreased component was determined to be nodakenin. The fermentation of the ten lactic acid bacteria demonstrated that the decomposable rate of these two compounds in fermented Samultang were different. Samultang fermented by L. plantarum showed the most remarkable changes. Conclusion : Nodakenetin was identified as bioconversion component after fermentation and L. plantarum was discovered the best bacteria to increase the component.

A Conversation Analysis of Communication between Patients with Dementia and Their Professional Nurses (치매 환자와 간호사의 의사소통에 관한 대화분석)

  • Yi, Myung-Sun;Yih, Bong-Sook
    • Journal of Korean Academy of Nursing
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    • v.36 no.7
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    • pp.1253-1264
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    • 2006
  • Purpose: The purpose of this study was to understand conversations and to identify typical conversational problems between nurses and patients with dementia. Method: A conversation analysis method was used. The data was collected in a geriatric institutional setting, using a videotape recorder, and transcribed. The transcribed data was analyzed in terms of expressions, contents, and relationships to identify communicative problems and their resolutions, Results: Among a total of 532 episodes, 440(82.7%) we're identified as nurse-involved episodes. In addition, 66 of the 440 episodes were selected based on the significance of the conversation. The communicative problems between nurses and patients in terms of expressions were identified as 'directive and authoritative expressions', 'emotional and competitive expressions', 'evasive and on-looking expressions', and 'excessive use of title only', such as calling them granny or grandpa without proper names. In terms of content and relationships, 'lack of themes in psychosocial areas' and 'nurse-led relations' were identified respectively as communicative problems. Conclusion: The results of this study will provide substantial guidelines for nurses in caring for elderly patients with dementia by deeply understanding linguistic structures and problems of everyday conversations between nurses and patients with dementia.

Breeding Difficulty of Marsh Crocodile (Crocodylus Palustris, Lesson, 1831) in Safari Park of Bangladesh

  • Masum, Kazi Mohammad;Rahman, Zahed Mohammad Malekur;Alamgir, Mohammed;Mamun, Abdullah Al;Abdullah-Al-Mamun, M.M.
    • Journal of Forest and Environmental Science
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    • v.28 no.4
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    • pp.220-226
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    • 2012
  • An assessment of Marsh crocodile's (Crocodylus palustris LESSON, 1831) habitat, its feasibility in supporting the total population, feeding, hatching difficulty and variations in egg and clutch size of Marsh crocodile were carried out in Bangabandhu Sheikh Mujib Safari Park for one year (July 2010 to June 2011). Major problem identified was the breeding failure, i.e. till now no hatching were found. Mainly two local weather variables (average temperature and humidity) were identified as the most obstructing parameters. Higher temperature (average $37.03^{\circ}C$) and Lower Humidity (average 75.33%) in comparison with standard range of incubation temperature and humidity for Marsh crocodile were seems to be vital reason for the failure of hatching. Understanding of the identified problem of this paper can direct the wildlife conservationist and the policy maker to take proper action for conserving the Marsh Crocodile in Bangladesh.

Validation of the Nursing Outcomes Classification on Cerebrovascular Patients (뇌혈관질환자에게 적용가능한 간호결과 분류체계의 타당성 검증)

  • Kim, Young-Hwa;So, Hyang-Sook;Lee, Eun-Joo;Ko, Eun
    • Korean Journal of Adult Nursing
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    • v.20 no.3
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    • pp.489-499
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    • 2008
  • Purpose: The purpose of this study was to assess the importance and contribution of 9 nursing outcomes and their indicators that could be applied to cerebrovascular patients. Methods: Data were collected from 175 neurosurgical nurses working at two university affiliated hospitals and five secondary hospitals located in Gwang-ju. The Fehring method was used to estimate outcome content validity(OCV) and outcome sensitivity validity(OSV) of nursing outcomes and their indicators. Stepwise regression was used to evaluate relationship between outcome and its indicators. Results: The core outcomes identified by the OCV were Tissue Perfusion: Cerebral, Nutritional Status, Neurological Status, and Wound Healing: Primary Intention, whereas highly supportive outcomes identified by the OSV were Oral Health, Self-Care: ADL, and Nutritional Status. All the critical indicators selected for Fehring method were not included in stepwise regression model. By stepwise regression analysis, the indicators explained outcomes from 19% to 52% in importance and from 21% to 45% in contribution. Conclusion: This study identified core and supportive outcomes and their indicators which could be useful to assess the physical status of cerebrovascular patients. Further research is needed for the revision and development of nursing outcomes and their indicators at neurological nursing area.

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The Effect of Marital Conflicts Perceived by Fathers on Preschoolers' Behavior Problems: Mediating Effect of Paternal Child Rearing Behavior (아버지가 지각한 부부갈등이 유아의 문제행동에 미치는 영향: 아버지 양육행동의 매개효과)

  • Kim, Lee Sook
    • Korean Journal of Childcare and Education
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    • v.12 no.2
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    • pp.127-142
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    • 2016
  • The purpose of this study is to analyze the effect of marital conflicts perceived by fathers on preschoolers' behavior problems, and to investigate the mediating effect of paternal child rearing behavior. This study used survey data on 4 year-old infants which was conducted in 2012(5th year) by KICCE. Correlation analysis and structural equation were conducted using SPSS 23 and AMOS 23 for the data analysis, and the results of this study are as follows. First, marital conflicts perceived by fathers and preschoolers behavior problems showed a positive correlation, and they especially showed a greater positive correlation with offensive behavior, anxiety/depression, and other problems. On the other hand, it was identified that paternal child rearing behavior has a negative correlation with marital conflict and preschoolers' behavior problems when paternal child rearing behavior is compassionate and dominant. Secondly, it was identified that warm parenting behavior of fathers mediates the effect of marital conflicts on preschoolers' behavior problems. However, it was verified that the mediating effect of controlling parenting behavior is not significant. On the other hand, it was identified that the mediating effect of warm parenting behavior of fathers has a greater effect on internalized problems than external problems.

A Study on the Hazard Factor of Packaged Hydrogen Station by Failure Mode & Effects Analysis (패키지형 수소충전소의 고장형태별 영향 분석)

  • SEO, DOO HYOUN;RHIE, KWANG WON;KIM, TAE HUN
    • Journal of Hydrogen and New Energy
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    • v.31 no.1
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    • pp.65-72
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    • 2020
  • In this study, the purpose is to identify the risks of the facilities of packaged hydrogen stations. As a risk identification method, failure mode & effect analysis (FMEA), a qualitative risk assessment, was used to analyze failure mode and effects of component of each facility. The analysis criteria were used to derive the risk priority number (RPN) using the 5-point method according to severity, incidence, and detectability. The study analyzed a total of 141 components of 23 types that can be identified on the design of the packaged hydrogen filling station. As a result, 683 types of failures and their causes and effects were identified. and the RPN was number of a total of 1,485. Of these, 10 failure types with a RPN value of 40 or more were deemed necessary. In addition, a list of failure types with a severity score of 5 was identified and analyzed.

Genomic Regions associated with Necrotic Enteritis Resistance in Fayoumi and White Leghorn Chickens

  • Kim, Eui-Soo;Lillehoj, Hyun S.;Sohn, Sea Hwan;Hong, Yeong Ho
    • Korean Journal of Poultry Science
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    • v.42 no.1
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    • pp.27-32
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    • 2015
  • In this study, we used two breeds of chicken to identify genomic regions corresponding to necrotic enteritis (NE) resistance. We scanned the genomes of a resistant and susceptible line of Fayoumi and White Leghorn chickens (20 birds/line) using a chicken 60 K Illumina SNP panel. A total of 235 loci with divergently fixed alleles were identified across the genome in both breeds; particularly, several clusters of multiple loci with fixed alleles were found in five narrow regions. Moreover, consensus 15-SNP haplotypes that were shared by the resistant lines of both breeds were identified on chromosomes 3, 7 and 9. Genes responsible for NE resistance were identified in chicken lines selected for resistance and susceptibility. Annotation of the regions spanning clustered divergently fixed regions revealed a set of interesting candidate genes such as phosphoinositide-3-kinase, regulatory subunit 5, p101 (PIK3R5) and inositol 1,4,5-trisphosphate receptor 1 (ITPR1), which participate in immune response. Consensus haplotypes were found in regions containing possibly relevant genes, such as myostatin and myosin, which play important roles in muscle development. Thus, genome scans of divergent selection in multiple chicken lines and breeds can be used to identify genomic regions associated with NE resistance.

Reduced Order Identification and Stability Analysis of DC-DC Converters

  • Ali, Husan;Zheng, Xiancheng;Wu, Xiaohua;Zaman, Haider;Khan, Shahbaz
    • Journal of Power Electronics
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    • v.17 no.2
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    • pp.453-463
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    • 2017
  • This paper discusses the measurement of frequency response functions for various dc-dc converters. The frequency domain identification procedure is applied to the measured frequency responses. The identified transfer functions are primarily used in developing behavioral models for dc-dc converters. Distributed power systems are based upon such converters in cascade, parallel and several other configurations. The system level analysis of a complete system becomes complex when the identified transfer functions are of high order. Therefore, a certain technique needs to be applied for order reduction of the identified transfer functions. During the process of order reduction, it has to be ensured that the system retains the dynamics of the full order system. The technique used here is based on the Hankel singular values of a system. A systematic procedure is given to retain the maximum energy states for the reduced order model. A dynamic analysis is performed for behavioral models based on full and reduced order frequency responses. The close agreement of results validates the effectiveness of the model order reduction. Stability is the key design objective for any system designer. Therefore, the measured frequency responses at the interface of the source and load are also used to predict stability of the system.

Identification of New Potential APE1 Inhibitors by Pharmacophore Modeling and Molecular Docking

  • Lee, In Won;Yoon, Jonghwan;Lee, Gunhee;Lee, Minho
    • Genomics & Informatics
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    • v.15 no.4
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    • pp.147-155
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    • 2017
  • Apurinic/apyrimidinic endonuclease 1 (APE1) is an enzyme responsible for the initial step in the base excision repair pathway and is known to be a potential drug target for treating cancers, because its expression is associated with resistance to DNA-damaging anticancer agents. Although several inhibitors already have been identified, the identification of novel kinds of potential inhibitors of APE1 could provide a seed for the development of improved anticancer drugs. For this purpose, we first classified known inhibitors of APE1. According to the classification, we constructed two distinct pharmacophore models. We screened more than 3 million lead-like compounds using the pharmacophores. Hits that fulfilled the features of the pharmacophore models were identified. In addition to the pharmacophore screen, we carried out molecular docking to prioritize hits. Based on these processes, we ultimately identified 1,338 potential inhibitors of APE1 with predicted binding affinities to the enzyme.

Phylogenetic Analysis of Mitochondrial DNA Control Region in the Swimming Crab, Portunus trituberculatus

  • Cho, Eun-Min;Min, Gi-Sik;Kanwal, Sumaira;Hyun, Young-Se;Park, Sun-Wha;Chung, Ki-Wha
    • Animal cells and systems
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    • v.13 no.3
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    • pp.305-314
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    • 2009
  • The control region of mitochondrial DNA (13516-14619) is located between srRNA and $tRNA^{lle}$ gene in swimming crab, Portunus trituberculatus. The present study was investigated the genetic polymorph isms of the control region in samples of P. trituberculatus collected at coastal waters of the Yellow Sea in Korea. A total of 300 substitution and indel polymorphic sites were identified. In addition to SNPs and indel variation, a hypervariable microsatellite motif was also identified at position from 14358 to 14391, which exhibited 10 alleles including 53 different suballeles. When the hypervariable microsatellite motif was removed from the alignment, 95 haplotypes were identified (93 unique haplotypes). The nucleotide and haplotype diversities were ranged from 0.024 to 0.028 and from 0.952 to 1.000, respectively. The statistically significant evidence for geographical structure was not detected from the analyses of neighbor-joining tree and minimum-spanning network, neither. This result suggest that population of P. trituberculatus are capable of extensive gene flow among populations. We believed that the polymorph isms of the control region will be used for informative markers to study phylogenetic relationships of P. trituberculatus.