• ALGAE
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• v.37 no.4
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• pp.317-329
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• 2022

Carotenoids are effective antioxidants that are found in various photosynthetic organisms. Marine microalgae are an advantageous bioresource for carotenoid production because they do not compete with other crops for freshwater and arable land. This study reports a newly isolated Dunaliella strain from the Geumhong Saltpan on Yeongjong Island, West Sea, Korea. The new strain was isolated and classified as Dunaliella salina through phylogenetic analysis and was named the OH214 strain (Deposit ID: KCTC14434BP). The newly isolated strain can survive in a wide range of NaCl concentrations (0.3-5.0 M NaCl), but grows well in 0.6 to 1.5 M NaCl culture medium. Under high-light conditions (500 ± 10 μmol photons m^-2 s^-1), the cells accumulated three times more β-carotene than under low-light conditions (50 ± 5 μmol photons m^-2 s^-1). The cells accumulated 2.5-fold more β-carotene under nitrogen-deficient (1 mM KNO₃) conditions (3.24 ± 0.36 ㎍ 10⁶ cells^-1) than in nitrogen-sufficient conditions (>5 mM KNO₃). The lutein content under nitrogen-deficient conditions (1.73 ± 0.09 ㎍ 10⁶ cells^-1) was more than 24% higher than that under nitrogen-sufficient conditions. Under the optimized culture condition for carotenoid induction using natural seawater, D. salina OH214 strain produced 7.97 ± 0.09 mg g DCW^-1 of β-carotene and 4.65 ± 0.18 mg g DCW^-1 of lutein, respectively. We propose that this new microalga is a promising strain for the simultaneous production of β-carotene and lutein.

• Analytical Science and Technology
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• v.36 no.1
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• pp.32-43
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• 2023

This study reports for the first time about a stability indicating RP-HPLC method for qualitative and quantitative determination of acalabrutinib in bulk and dosage form and in presence its impurities 1, 2 and 3. The chromatographic separation was carried on Zorbax XDB-C18 (250×4.6 mm; 5 µ id) as stationary phase, Phosphate buffer pH 6.4 and methanol 80:20 (v/v) as mobile phase at a flow rate of 1.0 mL/min, UV detection was carried at wavelength of 238 nm and the analysis was completed with a run time of 15 min. In these conditions the retention time of acalabrutinib and its impurities 1, 2 and 3 was observed to be 3.50, 4.83, 8.40 and 9.93 min respectively. The method was validated for system suitability, range of analysis, precision, specificity, stability and robustness. Spiked recovery at 50 %, 100 % and 150 % was carried for both standard and impurities and the acceptable % recovery of 98-102 was observed for acalabrutinib and both impurities studied and the % RSD in each spiked level was found to be less than 2. Stability tests were done through exposure of the analyte solution to five different stress conditions i.e expose to 1N hydrochloric acid, 1 N sodium hydroxide, 3 % peroxide, 80 ℃ temperature and UV radiation at 254 nm. In all the degradation condition, standard drug acalabrutinib was detected along with both the impurities studied and the degradation products were successfully separated. In the formulation analysis there is no other chromatographic detection of other impurities and formulation excipients. Hence the developed method was found to be suitable for the quantification of acalabrutinib and can separate and analyse impurities 1 and 2.

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.69-74
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• 2001

Purpose: $^{99m}Tc$-HMPAO is a radiopharmaceutical for imaging cerebral blood flow. HMPAO (RR, SS)-4.8-diaza-3,6,6,9-tetramethylundecan-2,10- dione bisoxime) has three stereoismers such as, meso-. d-, and l-HMPAO. Techentium complexes of meso-HMPAO and d,l-HMPAO are known to have different in vivo brain uptakes. In this study, enantiomers of HMPAO (d-HMPAO and l-HMPAO) were separated from d,l-HMPAO. These enantiomers were labeled with $^{99m}Tc$ and the biodistribution studies were performed in mice. Materials and Methods: An intermediate imine product was produced from 2,3-butanedione monooxime and 2,2-dimethyl-1,3-propanediamine (54% yield) and was reduced into a mixture of three isomers (35% yield). The meso-isomer was separated from d,l-mixture by repeated fractional crystallization (11 % yield). The d- and l-enantiomers were subsequently separated by co-crystallization with optical isomers of tartaric acid (25% and 5% yield. respectively). Each enantiomeric HMPAO was labeled with $^{99m}Tc$ by reacting with $SnCI_2{\cdot}2H_2O\;and\;^{99m}Tc$-pertechnetate. Biodistribution study was performed 1 hr after tail vein injection to ICR mice. Results: Radiochemical purities of each compound were over 80%. In biodistribution study. the brain uptakes of d,l- d- and l-form were 1.34, 1.12 and 1.67% ID/g, respectively. In case of l-lsomer the brain uptake was higher (1.5 fold) than d-isomer. Conclusion: We successfully purified each enantiomeric HMPAO. In biodistribution study of stereoismers of $^{99m}Tc$-HMPAO in mice, l-HMPAO may show better brain image than d,l-HMPAO which was supplied in a commercial kit.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.519-526
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• 2008

To improve the quality of traditional kochujang, strains with high protease and amylase activity were isolated and identified from Sunchang traditional kochujang. Twenty-three strains strongly producing protease and 16 strains strongly producing $\alpha$- and $\beta$-amylase were isolated by using 1% isolated soy protein agar medium and 2% starch agar medium, respectively. Protease activities of the IA7, I5, and IA2 strain were 22.5, 21.2, and 20.6 unit/mL, respectively, and were higher than those of the other strains. Stains with high $\alpha$-amylase activity included K9 (967.8 unit/mL), K14 (828.3 unit/mL), K13 (662.5 unit/mL), K8 (601.5 unit/mL), and K11 (405.9 unit/mL). The $\beta$-amylase activity of the K11 strain was the highest, 34.3 unit/mL, among the isolated strains. Based on morphological, physiological properties, and API 50CHB-kit test for assimilation of 49 carbohydrates, 8 strains selected according to protease, $\alpha$-amylase, and $\beta$-amylase activities were tentatively identified as Bacillus megaterium (IA2), Bacillus subtilis (IA7, 15), Bacillus amyloliquefaciens (K8, K9, K11, and K13), and Bacillus stearothermophillus (K14). The IA7, 15, and K11 strains were finally identified as B. subtilis (99% ID) based on 16S rDNA sequencing.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.2
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• pp.136-144
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• 2005

GIS analyzes various topographic feature in three dimensions using triangulated irregular network (TIN). This paper proposed a method that can analyze topographic features effectively in three dimensions. Method developed in this study can calculate earth-volume, the inundation volume, storage volume etc, effectively. We could reduce error than existing volume calculation methods in computing volume in addition to water level's change about each. Also, the develop method can improve accuracy in measuring the storage of reservoirs. Main result of this paper id to develop a efficient calculation method using contour and elevation data on digital map.

• Journal of the Korea Convergence Society
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• v.7 no.4
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• pp.133-140
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• 2016

This paper suggests designing how to acquire English vocabularies on the smart devices based on the research that a ground-breaking English Vocabulary Learning Contents needs developing. The method makes it possible to develop the contents which helps the learners to master English vocabularies effectively on the smart phone. The core idea of this paper is as in the following: 1) English learners learn 30 vocabularies for three minutes 10 times (one is for a new learning and the other nine ones are for reviews about the first learning) a day. 2) Considering Ebbinghaus Forgetting Curve, the reflection study proposes to provide the learners with three times' reviews: one day, 10days, and 30days later from which they learn the first 30 vocabularies. This contents is mainly made up of 5 developing sections (1)to generate App ID, (2)to access App, (3)to set up Alarm, (4)to process Word learning, and (5)to monitor the result of learning. This proposed idea is optimized to enhance the memory by Ebbinghaus Periodic Repetitive Method, which makes the learners satisfied with their English vocabulary learning.

• Journal of Korea Society of Digital Industry and Information Management
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• v.5 no.3
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• pp.175-184
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• 2009

We are entering into a era of enterprise computing that is characterized by an emphasis on broadband convergence, knowledge s haring, and calm services. Some people refer to this as the "ubiquitous" computing because its focus is on a high degree of connectivity between a company and its customers, suppliers, and channel partners. Ubiquitous computing technology, "RF" stands for "radio frequency"; the "ID" means "identifer". The tag itself of a computer chip and an antenna. The shortest metaphor is that RFID is like a bar-code but is read with an electromagnetic field rather than by a laser beam. Much has already been written about the use of RFID. But there is no has written about the use of RFID in the convention industry. Therefore this study have specific objectives as follows. 1. To give details on the use of RFID in convention. 2. To introduces the key concepts behind RFID technology. 3. To identify advantage & disadvantage of RFID technology using a BEXCO CASE study. 4. To study on ubiquitous convention using RFID and effective operation methods such as entrance identification system, session management, machine management, CRM management, visitor management, and contents management. This results provide into the current status of ubiquitous computing technology in convention industries. Specific advantages by using ubiquitous computing technology(RFID) are one-stop differentiate service, wireless internet service, use of visitor management system, entrance by tag, and U-logistics. On other side, disadvantages are security, stabilization of RFID system, higher price of RFID tag, and commercial scale. Convention by using of RFID technology is currently at an early stage. Convention company as BEXCO need to have the capabilities to adapt, to customize, to commercialize, and to modify technology to suit our circumstances.

• Journal of Sensor Science and Technology
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• v.10 no.3
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• pp.187-195
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• 2001

A wireless telemetry measurement system, using ISFETs, was developed for environmental monitoring applications, industrial processes, medical treatments and so on. This system is composed of the measurement part located at remote site and a personal computer(PC) which control whole instrument process. The measurement part transmits measured data to the PC by RF transceiver, and transmitted data are analyzed and handled in the PC. Proposed system utilizing time division multiplexing for the transmission of 3channel ISFET signal. The measurement part has an identification number(ID) so that the PC controls many measurement parts separately. Experiments were performed using pH-ISFETs, and the implemented system operates well within designed specification and accuracy of 0.1 pH.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.38 no.3
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• pp.211-219
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• 2001

This paper describes a read-only CMOS transponder IC for RFID applications. A full-wave rectifier implemented using NMOS transistors supplies the transponder with a dc supply voltage using the magnetic field generated from a reader. A 64-bit ROM has been designed for a data memory. Front-end impedance modulation and Manchester coding are used for transmitting the data from the transponder memory to the reader. A new damping circuit which has almost constant damping rate under the variations of the distance between the transponder and the reader has been employed for impedance modulation. The designed circuit has been fabricated using a 0.65${\mu}{\textrm}{m}$2-poly, 2-metal CMOS process. Die area is 0.9mm$\times$0.4mm. Measurement results show that it has a constant damping rate of around 20~25% and a data transmission rate of 3.9kbps at a 125KHz RF carrier. The power required for reading operation is about 100㎼. The measured reading distance is around 7cm.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.3
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• pp.183-188
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• 2017

This paper is for the a study on home healthcare system for a patient using SAW Tag. This system is useful when there's a patient in home. Many patients are wounded in restroom. But restroom is very private place and nobody want to be disturbed here. The place where the patient is most injured in the house is the toilet, and it is the point where the privacy of the individual should be kept and the patient's condition should be checked and cope with in case of emergency. And using saw tag, we can calculate position and ID through reflected rf signal. Therefore we propose a general home healthcare system. The management system utilized a seat bed system to monitor at-home patients. The system mounts a bio-signal measuring unit on a toilet seat, measures a bio-signal from the bio-signal measuring unit, and the measured bio-signal is transmitted to the main server through the user's smart phone. With proposed system, we can expand operation area to silver town where many patients are cared. In this paper we developed sensor system and saw tag positioning system and showed the result.