• Title/Summary/Keyword: Ice cooling

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Changes in the $Ca^{2+}$-, $Mg^{2+}$- dependent Adenosine Triphosphatase Activity and Ultrastructure of Marine Fishes by Partial Freezing II. Changes in ATPase Activity of Yellowtail Actomyosin during Cold Storage (해산어의 부분동결에 의한 $Ca^{2+}$-, $Mg^{2+}$- dependent Adenosine Triphosphatase 활성 및 근섬유의 미세구조 변화 II. 저온저장에 의한 방어 Actomyosin ATPase의 활성변화)

  • 박찬성;최경호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.4
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    • pp.349-355
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    • 1990
  • Actomyosine prepared from Yellowtail fish(seriola quinqueradiata) were stored at $0^{\circ}C$(ice-cooling) -3.5$^{\circ}C$(partial freeaing) and -2$0^{\circ}C$(freezing) Another actomyosin samples were prepared from the fish previously stored at the temperatures for a week as the maximum .Remaining activity of {{{{ {Ca }^{2+ } }}}}-and {{{{ {Mg }^{2+ } }}}}- dependent adenosine triphosphatase(ATPase) activity was measured fronm the actomyosin preparations. Specific activity of {{{{ {Mg }^{2+ } }}}}-ATPase of actomy-osin before storagew was 0.253$\mu$ mole pi/min/mg of protein and it was 1.5 times higher than that of {{{{ {Ca }^{2+ } }}}} -ATPase. The enzyme activities were markedly decreased during early period of storage. However no significant differences in the enzyme activity were revealed among the samples stored at different temperature. The enzyme of actomyosin prepared from the fish previously stored at the temperatures for a week revealed an acitivity of 2-3 times higher than that of freezing. Apparent denaturation constant of {{{{ {Mg }^{2+ } }}}} -ATPase of actomyosin was between 0.810-1.139 per day and it was about 1.5 times hgiher than that of {{{{ {Ca }^{2+ } }}}} -ATPase. But the constant of {{{{ {Mg }^{2+ } }}}} ATPase of actomyosin extracted from the fist stored for a week at each temperature was between 0.176-0.356 per day. This constant was 4 times higher than that of {{{{ {Ca }^{2+ } }}}}- ATPase in frozen stored fish. It was presumed from these results that denaturation of ATPase is largely accorded to the structural changes of actomyosin.

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Prediction of boil-off gas and boil-off rate in cargo tank of NGH carrier

  • Kang, Ho-Keunn;Kim, Dongeum;Kim, You-Taek;Park, Jung-Dae;Kang, Shin-Baek
    • Journal of Advanced Marine Engineering and Technology
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    • v.39 no.10
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    • pp.1002-1010
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    • 2015
  • Natural gas hydrates are newly emerging as an environment-friendly source of energy to substitute for fossil fuels in the 21stcentury.NGHs are reported to holds much amounts of natural gas (up to 182 standard volumes of gas per volume of hydrate); they are easy to store and safe to carry at about minus 20 degree Celsius under atmospheric pressure because of the self-preservation phenomenon of gas hydrates. The transporting method by gas-ice-hydrate ship carriers has been introduced and developed by a variety of industry and research institutions. Our team has been conducted to develop NGH total systems, including a breakthrough NGH carrier for sea transportation, since 2011. The NGH pellet carrier does not require a separate cooling system for cargo, and the initial temperature is maintained through insulation of the cargo tanks throughout the transport to the final destination. The heat conducted from the exterior and passing through the insulation material of the hull should be cut off as much as possible, but heat inflow inside the cargo tank from an external source is inevitable during transport. In this study, the heat transfer in a cargo tank of a 115K NGH carrier was analyzed through simulation with a commercial CFD code to estimate the boil-off gas/boil-off rate on the developed carrier and understand major hazards that could significantly impact the safety of the vessel.

Development the Technique for Fabrication of the Thermal Fatigue Crack to Enhance the Reliability of Structural Component in NPPs (원자력 구조재 신뢰성 향상을 위한 열피로 균열 시험편 제작 기법 개발)

  • Kim, Yong;Kim, Jae-Sung;Lee, Bo-Young
    • Journal of Welding and Joining
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    • v.26 no.2
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    • pp.43-49
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    • 2008
  • Fatigue cracks due to thermal stratification or corrosion in pipelines of nuclear power plants can cause serious problems on reactor cooling system. Therefore, the development of an integrated technology including fabrication of standard specimens and their practical usage is needed to enhance the reliability of nondestructive testing. The test material was austenitic STS 304, which is used as pipelines in the Reactor Coolant System of a nuclear power plants. The best condition for fabrication of thermal fatigue cracks at the notch plate was selected using the thermal stress analysis of ANSYS. The specimen was installed from the tensile tester and underwent continuos tension loads of 51,000N. Then, after the specimen was heated to $450^{\circ}C$ for 1 minute using HF induction heater, it was cooled to $20^{\circ}C$ in 1 minute using a mixture of dry ice and water. The initial crack was generated at 17,000 cycles, 560 hours later (1cycle/2min.) and the depth of the thermal fatigue crack reached about 40% of the thickness of the specimen at 22,000 cycles. As a results of optical microscope and SEM analysis, it is confirmed that fabricated thermal fatigue cracks have the same characteristics as real fatigue cracks in nuclear power plants. The crack shape and size were identified.

A Study on the Development of Dance Sportswear with Cool-touch Function (냉감 기능성 댄스스포츠 웨어 개발에 관한 연구)

  • Jun, Mi-Hwa;Jang, Jeong-Ah;Koo, Young-Seok
    • Fashion & Textile Research Journal
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    • v.22 no.1
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    • pp.66-75
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    • 2020
  • This study helps develop cool-touch functional dance sportswear. We suggest a draft design for dance sportswear that chooses appropriate cool-touch functional materials based on an investigation of the changes of body surface temperature before and after exercise, the physical properties of cool-touch materials on the market, and the preference for cooling tools. The results are as follows. First, cool-touch functional sportswear products on the market utilize materials such as PCM, Delta fabric, high gauge fabric, and ice chips as well as incorporate functions such as UV block and eyelets for enhanced breathability. Polyester and polyurethane fibers are mainly used for cool-touch functional sportswear. Second, the neck area showed the highest surface temperatures (32.7℃ and 32.1℃) before and after exercise. Body surface temperatures measured after exercise were also lower than temperatures measured before exercise when wearing dance sportswear. Third, as for the physical properties of cool-touch materials, material 1 showed amaximum drying speed (130 min), material 3 the best moisture absorption speed (122 × 132 min), and material 4 the best thermal conductivity (0.013 7 w/m·K). Fourth, a draft design for a cool-touch functional dance sportswear was suggested, including a neckband made of removable soft PVC material on the neck area and applying material 4 in F1, B4, S2 and lower arm areas and material 1 in the armpit area. Deodorant tape was also attached to the armpit area for added comfort and antibacterial deodorant effect.

Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing (고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법)

  • Choi, Yun-Joung;Lee, Kyung-Hwan;Je, A-Reum;Chae, Hee-Su;Jang, Ji-Hoon;Lee, Eun-Ji;Kweon, Hee-Seok
    • Applied Microscopy
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    • v.42 no.2
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    • pp.111-114
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    • 2012
  • The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

Controlled Partial Skin Thickness Burns: Rabbit Ear as a 2nd Degree Burn Wound Model for Studies of Topical Therapy

  • Cho, Lee Ae-Ri;Moon, Hee-Kyung
    • Journal of Pharmaceutical Investigation
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    • v.36 no.5
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    • pp.339-342
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    • 2006
  • This study was designed to prepare an animal model for partial thickness bum wound which can be employed for testing topical therapy. We first evaluated whether rabbit ear and mouse back skin wound model could differentiate the wound healing process in terms of degree of re epithelialization, required days for complete wound closure, presence of scarring. $2^{nd}$ degree wet bum were prepared on mouse back skin and rabbit ear by applying 5 mL hot water($85{\pm}0.1^{\circ}C$) for 7 see followed by 5 mL ice-cold 0.5% acrynol solution for cooling and disinfecting the inflicted area. After removing the dead epidermis layer at 24 hr, tested dressings were applied for specified time and wound progression was investigated. In mouse model, wound contraction was the primary wound closing mechanism, which is quite different from human wound healing process. In rabbit ear model, epidermal regeneration was the major wound healing process rather than wound contraction and the difference in wound healing property among tested dressings could be clearly demonstrated. A rabbit ear model could differentiate the wound progression among open, occluded and epidermal growth factor(EGF) treated wound. Four sites of circular wound(diameter: 1 cm) on the anterior part of rabbit ear could be employed for the comparative wound healing study. For obtaining reproducible bum wound, degree of bum depth and bum sites should be carefully controlled in addition, employing rabbits of same strain and weight. The result suggests that rabbit ear could be employed as a reliable and human-resembled wound model.

Studies on Cryo-preservation of Registered Strains of Lentinula edodes (표고 등록균주의 초저온 보존에 관한 연구)

  • Ryu, Sung-Ryul;Bak, Won-Chull;Koo, Chang-Duck;Ka, Kang-Hyeon
    • Journal of Korean Society of Forest Science
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    • v.98 no.1
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    • pp.115-124
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    • 2009
  • New strain needs to maintain desirable characteristics for long term when it was bred, but in lapse of time it degenerates into a bad condition. Therefore the influence of temperature on the viability and survival rates of Lentinula edodes strains were examined after cryopreservation. Also, liquid nitrogen preservation for L. edodes has been proved to be one of the most reliable method. However, a mechanical damage of strain is inevitable during cryopreservation of the fungus because the fungus is very sensitive to stress of cooling rate in the freezing process. So we tried to find out state change of L. edodes with a programmable freezer. L. edodes strains were preserved at $-20^{\circ}C$, $-80^{\circ}C$ and $-196^{\circ}C$ for 50 days. At $-20^{\circ}C$, its mycelial growth became extinct. When thawed, the growth of mycelia which were preserved at $-80^{\circ}C$ was fastest. Attempts were made to investigate viability of L. edodes strains after freezing at $-80^{\circ}C$ and $-196^{\circ}C$, respectively. As the result, more than 90% showed high survival rate of strains tested at $-80^{\circ}C$ and $-196^{\circ}C$. Mycelial growth between apical and basal parts of colony after freezing preservation for 50 days was compared. At apical and basal parts, the survival rates showed 100% at $-80^{\circ}C$, but 98% and 94% at $-196^{\circ}C$, respectively. We confirmed that the ice crystal formation temperatures of L. edodes strains were $-6.0^{\circ}C$ for Sanlim 1, $-5.5^{\circ}C$ for the Sanlim 2, $-4.0^{\circ}C$ for the Sanlim 3 and $-15.5^{\circ}C$ for the Sanzo 302. These results indicated that L. edodes strains showed completely different responses to the ice crystal formation. We knew the fact that even the same species, especially L. edodes, they displayed completely different responses to the same freezing condition. Also, this has nothing to do with the connection between temperature type and freezing point. And a protocol was tried to minimize state change of L. edodes strains using programmable freezer when they are frozen, but it was not effective on them.

Recent Progress in Air Conditioning and Refrigeration Research : A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2007 (설비공학 분야의 최근 연구 동향 : 2007년 학회지 논문에 대한 종합적 고찰)

  • Han, Hwa-Taik;Shin, Dong-Sin;Choi, Chang-Ho;Lee, Dae-Young;Kim, Seo-Young;Kwon, Yong-Il
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.20 no.12
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    • pp.844-861
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    • 2008
  • The papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during the year of 2007 have been reviewed. Focus has been put on current status of research in the aspect of heating, cooling, ventilation, sanitation and building environments. The conclusions are as follows. (1) The research trends of fluid engineering have been surveyed as groups of general fluid flow, fluid machinery and piping, etc. New research topics include micro nano fluid, micropump and fuel cell. Traditional CFD was still popular and widely used in research and development. Studies about fans and pumps were performed in the field of fluid machinery. Characteristics of flow and fin shape optimization are studied in the field of piping system. (2) The research works on heat transfer have been reviewed in the field of heat transfer characteristics, heat exchangers, and desiccant cooling systems. The research on heat transfer characteristics includes thermal transport in pulse tubes, high temperature superconductors, ground heat exchangers, fuel cell stacks and ice slurry systems. For the heat 'exchangers, the research on pin-tube heat exchanger, plate heat exchanger, condensers and gas coolers has been cordially implemented. The research works on heat transfer augmenting tubes have been also reported. For the desiccant cooling systems, the studies on the design and operating conditions for desiccant rotors as well as performance index are noticeable. (3) In the field of refrigeration, many papers were presented on the air conditioning system using CO2 as a refrigerant. The issues on the two-stage compression, the oil selection, and the appropriate oil charge were treated. The subjects of alternative refrigerants were also studied steadily. Hydrocarbons, DME and their mixtures were considered and various heat transfer correlations were proposed. (4) Research papers have been reviewed in the field of building facilities by grouping into the researches on heat and cold sources, air conditioning and air cleaning, ventilation and fire research including tunnel ventilation, flow control of piping system, and sound research with drain system. Main focuses have been addressed to the promotion of efficient or effective use of energy, which helps to save energy and results in reduced environmental pollution and operating cost. (5) Studies were mostly focused on analyzing the indoor environment in various spaces like cars, old tombs, machine rooms, and etc. in an architectural environmental field. Moreover, subjects of various fields such as the evaluation of noise, thermal environment, indoor air quality and development of energy analysis program were researched by various methods of survey, simulation, and field experiment.

Study of East Asia Climate Change for the Last Glacial Maximum Using Numerical Model (수치모델을 이용한 Last Glacial Maximum의 동아시아 기후변화 연구)

  • Kim, Seong-Joong;Park, Yoo-Min;Lee, Bang-Yong;Choi, Tae-Jin;Yoon, Young-Jun;Suk, Bong-Chool
    • The Korean Journal of Quaternary Research
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    • v.20 no.1 s.26
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    • pp.51-66
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    • 2006
  • The climate of the last glacial maximum (LGM) in northeast Asia is simulated with an atmospheric general circulation model of NCAR CCM3 at spectral truncation of T170, corresponding to a grid cell size of roughly 75 km. Modern climate is simulated by a prescribed sea surface temperature and sea ice provided from NCAR, and contemporary atmospheric CO2, topography, and orbital parameters, while LGM simulation was forced with the reconstructed CLIMAP sea surface temperatures, sea ice distribution, ice sheet topography, reduced $CO_2$, and orbital parameters. Under LGM conditions, surface temperature is markedly reduced in winter by more than $18^{\circ}C$ in the Korean west sea and continental margin of the Korean east sea, where the ocean exposed to land in the LGM, whereas in these areas surface temperature is warmer than present in summer by up to $2^{\circ}C$. This is due to the difference in heat capacity between ocean and land. Overall, in the LGM surface is cooled by $4{\sim}6^{\circ}C$ in northeast Asia land and by $7.1^{\circ}C$ in the entire area. An analysis of surface heat fluxes show that the surface cooling is due to the increase in outgoing longwave radiation associated with the reduced $CO_2$ concentration. The reduction in surface temperature leads to a weakening of the hydrological cycle. In winter, precipitation decreases largely in the southeastern part of Asia by about $1{\sim}4\;mm/day$, while in summer a larger reduction is found over China. Overall, annual-mean precipitation decreases by about 50% in the LGM. In northeast Asia, evaporation is also overall reduced in the LGM, but the reduction of precipitation is larger, eventually leading to a drier climate. The drier LGM climate simulated in this study is consistent with proxy evidence compiled in other areas. Overall, the high-resolution model captures the climate features reasonably well under global domain.

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Effect of Varying Excessive Air Ratios on Nitrogen Oxides and Fuel Consumption Rate during Warm-up in a 2-L Hydrogen Direct Injection Spark Ignition Engine (2 L급 수소 직접분사 전기점화 엔진의 워밍업 시 공기과잉률에 따른 질소산화물 배출 및 연료 소모율에 대한 실험적 분석)

  • Jun Ha;Yongrae Kim;Cheolwoong Park;Young Choi;Jeongwoo Lee
    • Journal of the Korean Institute of Gas
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    • v.27 no.3
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    • pp.52-58
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    • 2023
  • With the increasing awareness of the importance of carbon neutrality in response to global climate change, the utilization of hydrogen as a carbon-free fuel source is also growing. Hydrogen is commonly used in fuel cells (FC), but it can also be utilized in internal combustion engines (ICE) that are based on combustion. Particularly, ICEs that already have established infrastructure for production and supply can greatly contribute to the expansion of hydrogen energy utilization when it becomes difficult to rely solely on fuel cells or expand their infrastructure. However, a disadvantage of utilizing hydrogen through combustion is the potential generation of nitrogen oxides (NOx), which are harmful emissions formed when nitrogen in the air reacts with oxygen at high temperatures. In particular, for the EURO-7 exhaust regulation, which includes cold start operation, efforts to reduce exhaust emissions during the warm-up process are required. Therefore, in this study, the characteristics of nitrogen oxides and fuel consumption were investigated during the warm-up process of cooling water from room temperature to 88℃ using a 2-liter direct injection spark ignition (SI) engine fueled with hydrogen. One advantage of hydrogen, compared to conventional fuels like gasoline, natural gas, and liquefied petroleum gas (LPG), is its wide flammable range, which allows for sparser control of the excessive air ratio. In this study, the excessive air ratio was varied as 1.6/1.8/2.0 during the warm-up process, and the results were analyzed. The experimental results show that as the excessive air ratio becomes sparser during warm-up, the emission of nitrogen oxides per unit time decreases, and the thermal efficiency relatively increases. However, as the time required to reach the final temperature becomes longer, the cumulative emissions and fuel consumption may worsen.