• Applied Biological Chemistry
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• v.50 no.2
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• pp.101-106
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• 2007

Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

• The Korean Journal of Mycology
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• v.41 no.3
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• pp.137-141
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• 2013

Diversity of endophytic fungi of two species associated with host plants, Pinus densiflora and Larix kaempferi in Mt. Oser were investigated. Endophytic fungi were isolated from surface sterilized leaves of the 13 host plants and identified by morphological traits and rDNA ITS sequences analysis. Totally 37 isolates were discovered and identified into 17 taxa. Of them, 59% isolates belonged to Leotiomycetes, 30% isolates Sordariomyetes, 8% isolates Dothideomycetes and 3% isolates Agaricomycetes. Results showed a similar diversity pattern with previous studies. Also, species diversity (H') of endophytic fungi isolated from L. kaempferi was higher than that from P. densiflora. The endophytic species composition showed significant differences between host plants. Particularly, taxon of Lophodermium is a major genus in the host plants. However, critical research of the endophytic species diversities within the genus in Korea is required. The communities of endophytic fungi isolated in this study showed differences in diversities and species composition between host plants.

• Journal of Mushroom
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• v.21 no.4
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• pp.228-240
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• 2023

To understand microorganism effects on wild mushroom fruiting bodies, we investigated the fungi in hyphosphere soil supporting wild mushroom species Cortinarius violaceus, Amanita hemibapha, Laccaria vinacelavellanea, and Amanita verna found in the Gotjawal area of Jeju Island. Fungal species identification based on morphological traits and molecular analysis of ITS, LSU rDNA, and β-tubulin gene sequences resulted in isolation and identification of eleven fungal species previously unrecorded in Korea. These newly-recorded species are: Arthrinium kogelbergensis, Kalmusia longisporum, Keithomyces carneum, Neopyrenochaeta cercidis, Penicillium ranomafanaense, Phomatodes nebulosa, Pyrenochaeta nobilis, Tolypocladium album, Talaromyces kendrickii, Talaromyces qii, and Umbelopsis gibberispora, and their morphological characteristics and phylogenetic positions are described.

• The Plant Pathology Journal
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• v.25 no.3
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• pp.236-240
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• 2009

Tylenchulus semipenetrans, citrus nematode is an important phytopathogenic nematode and responsible for serious damage on citrus. However, little information is available about genetic variability of T. semipenetrans among different populations with variation of conventional diagnostic characteristics. In this study, we compared the morphometric and genetic characteristics among different populations. The mature female of T. semipenetrans collected in this study had thicker cuticle than those in the previous studies. In comparative sequence analysis of T. semipenetrans populations obtained from Jeju in Korea, we observed genetic variations within clones generated from single individuals. To determine whether variability among copies of nuclear ribosomal DNA sequences exists in the genome of T. semipenetrans, PCR-RFLP technique from individuals of Korean isolates with MseI and MspI restriction enzymes was used to prove experimentally that all populations have intra-specific variations. Restriction enzyme digestion created several fragments on 3.0% agarose gel corresponding to several haplotypes in all populations, though some populations displayed fragment deletion. The total length of fragments was larger than before digestion, indicating sequence heterogeneity within the genome of T. semipenetrans.

• ALGAE
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• v.31 no.4
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• pp.303-315
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• 2016

Compsopogon specimens collected in China were examined based on morphology and DNA sequences. Five molecular markers from different genome compartments including rbcL, COI, 18S rDNA, psbA, and UPA were identified and used to construct a phylogenetic relationship. Phylogenetic analyses indicated that two different morphological types from China clustered into an independent clade with Compsopogon specimens when compared to other global samples. The Compsopogon clade exhibited robust support values, revealing the affiliation of the samples to Compsopogon caeruleus. Although the samples were distributed in a close geographical area, unexpected sequence divergences between the Chinese samples implied that they were introduced by different dispersal events and from varied origins. It was speculated that Compsopogon originated in North America, a portion of the Laurentia landmass situated in the Rodinia supercontinent at approximately 573.89-1,701.50 million years ago during the Proterozoic era.Although Compsopogonhad evolved for a rather long time, genetic conservation had limited its variability and rate of evolution, resulting in the current monospecific global distribution. Additional global specimens and sequence information were required to increase our understanding of the evolutionary history of this ancient red algal lineage.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2003.11a
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• pp.81-85
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• 2003

In round numbers the 100 strains of entomopathogenic nematodes were isolated through the investigation of cultivated including the ginseng forming cultivated and forest soil samples by silkworm trap. The 28 strains of nematodes were selected among the isolated entomopathogenic nematodes that were confirmed the pathogenicity against Holotrichia morosa, Hoiotrichia diomphalia and Ectinus sericeus, the pest of korean ginseng and silkworm. Pathogenicity of the 2025, 2027, 2028, 2034, 2039 and 2057 strains was excellent. Selected entomopathogenic nematodes are classified of two species by morphologiacl and molecular studies, which were Sterinernma carpocapsae sp. and Diplogaster lethier sp.. Diagnostic characters include body length, lateral field pattern, tail shape and so on. The DNA sequences of the ITS region of rDNA shows similar to S. carpocapsae and .D. lethier. Isolated entomopathogenic nematodes were demonstrates that are quite within the realms of possibility for biological control agents of the pests of Korean Ginseng.

• Journal of Veterinary Clinics
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• v.25 no.3
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• pp.207-210
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• 2008

Trichophyton erinacei is a dermatophyte pathogen that infects both humans and hedgehogs. A two-month old female four-toed hedgehog presented to the Chonbuk Animal Medical Center with pruritus, excoriation and crust on her face for ten days. The owner of the hedgehog also exhibited the clinical signs of scaly erythema with fine vesicles on her neck. A presumptive diagnosis of dermatophytosis was made based on the results of an acetate tape preparation in which hyphae and chains of arthroconidia were observed. The crusts from the lesions were then cultured on Sabouraud Dextrose Agar for identification. After 10 days of incubation, downy colored colonies that had a central umbo with a white granular surface and a yellow pigment ring in the reverse were observed. Microscopic analysis revealed the presence of numerous teardrop shaped microconidia singly attached to the sides of the hyphae. In addition, 2-6 roomed macroconidia that were somewhat irregular in shape and size were present, and abundant intermediate sized spores were observed between the micro and macro conidia. To confirm that the culture was T. erinacei, the internal transcribed spacer region of the 5.8S phase of the ribosomal RNA gene (ITS1-5.8S-ITS2 rDNA) was amplified by PCR and then sequenced. A 679-base pair fragment of DNA was then compared with sequences in GenBank and found to be 99% homologous with sequences of T. erinacei (Z97997 and Z97996. The clinical signs were resolved after four weeks of treatment with oral and topical ketoconazole and chlorhexidine. To the best of our knowledge, this represents the first case of T. erinacei isolated from a four-toed hedgehog in Korea.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.677-684
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• 2001

To isolate mutanse-producing bacteria, soil samples were collected from several areas in chonnam Province, South Korea. A total of 70 strains of actinomycetes were isolated from the soil samples. All isolated actinomycetes were inoculated on mutanase screening media to identify new bacterial strains producing mutanase activity. One strain in particular exhibited a strong mutanase-producing activity, and was identified as Microbispora rosea based on its morphological, cultural, and physiological characteristics, and also by 16S rDNA sequences.

• Research in Plant Disease
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• v.22 no.3
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• pp.190-193
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• 2016

In 2010 and 2012, damping-off symptoms were found on melon seedlings grown in Yeongam and Suncheon, Korea. Water-soaked and discolored lesions appeared on the lower stems of the infected plants. The diseased plants became wilted and stunted, and eventually collapsed. On the basis of morphological characteristics and molecular analyses of internal transcribed spacer (ITS) rDNA and cytochrome oxidase II (cox2) sequences, the causal organism was identified as Pythium spinosum. The isolates were pathogenic to melon under pot conditions. To our knowledge, this is the first report of P. spinosum causing damping-off on melon in Korea.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.360-364
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• 2016

Fungal strain KNU16-005 was isolated from field soil in Jeollabuk-do, Korea. Based on its morphological characteristics and phylogenic analysis using internal transcribed spacer regions of rDNA, ${\beta}$-tubulin and translation elongation factor 1-alpha gene sequences of KNU16-005 were identified as Neopestalotiopsis australis. This species has not been previously reported in Korea.