• Journal of Life Science
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• v.16 no.3 s.76
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• pp.477-484
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• 2006

This study was performed to know the potentialities of the fruits of Opuntia ficus-indica, as a medium for mushroom mycelial culture. Five mushroom mycelial (Agrocus blazei, Grifola frondosa, Hericium erinaceum, Innonotus obliquus, Phellinus linteus) were frown on the malt extract broth (MEB) and the cactus broth medium (CB). The submerged culture mixtures were extracted using equal volume of ethyl acetate, and their extract yields, total polyphenol contents, and some physiological activities were compared with each other Each extract from mycelial culture grown on CB medium showed remarkable enhancement in physiological activities compared with each counterpart grown on MEB. Among five mycelial cultures grown on CB medium, the extract yield and polyphenyl content were highest in the extract from Grifola frondosa (extract yield, 0.4 g/L and polyphenol content, 22.7%). Also, the extracts from Grifola frondosa showed the highest physiological activities, such as DPPH radical scavenging ($IC_{50}=362.9{\mu}g/ml$), xanthine oxidase inhibition (about 80% at $500{\mu}g/ml$), and superoxide radical scavenging (about 80% at $500{\mu}g/ml$), and NO production inhibition ($IC_{50}=43.1{\mu}g/ml$) in LPS-stimulated RAW 264.7 cells. This result suggests that the fruit of Opuntia ficus-indica can be used as a culture medium for improving the functional properties of various mushroom mycelia.

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.296-303
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• 2007

Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity ($RC_{50}=3.15\;{\mu}g/m{\ell}$) among the fractions. The isolated compounds were analyzed by $^1H$ and $^{13}C$ NMR, IR, UV/VIS, MS spectrum data and identified as catechin, ${\rho}-Hydroxyphenethyl$ alcohol $1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$. The compounds have shown strong antioxidant activity, with similar activity to BHA ($RC_{50}=2\;{\mu}g/m{\ell}$). Especially, ${\rho}-Hydroxyphenethyl$ alcohol 1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$ was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in $125\;{\mu}g/m{\ell}$. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting $63\;{\mu}g/m{\ell}$ of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.2
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• pp.200-207
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• 2015

Various natural products or their derivatives, mostly originating from plants, fungi, and bacteria, have been exploited as therapeutic drugs to treat various human diseases. In addition to previously explored organisms, research on natural compounds has now expanded into unexamined living organisms in order to identify novel bioactive substances. Here, we determined whether or not the larval form of the mealworm beetle Tenebrio molitor, a species of darkling beetle, contains cytotoxic substances that exclusively affect cancer cell viability. Ethanol extract and its solvent partitioned fractions, hexane and ethyl acetate fractions, showed anticancer effects against various human cancer cells derived from the prostate (PC3 and 22Rv1), cervix (HeLa), liver (PLC/PRF5, HepG2, Hep3B, and SK-HEP-1), colon (HCT116), lung (NCI-H460), breast (MDA-MB231), and ovary (SKOV3). Cell death induced by the fractions was a mix of apoptosis, necrosis, and autophagy. The hexane fraction was administered intraperitoneally to nude mice bearing a hepatocellular carcinoma SK-HEP-1 and showed inhibition of tumor growth in vivo. Therefore, we concluded that worm extracts contain cytotoxic substances, which can be enriched by proper fractionation protocols, and further separation and purification could lead to the identification of novel molecules to treat human cancers.

• Journal of Korean Society of Forest Science
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• v.106 no.4
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• pp.487-496
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• 2017

The marketization in North Korea is spreading rapidly. This study proposes forest rehabilitation strategy for North Korea in light of their major shift toward market economy. This current trend of marketization in North Korea is now affecting the forest sector, especially the way the residents utilize small forest land. For analyzing the influence of marketization on forest management in North Korea, we reviewed the official documents issued by North Korea and related materials of North Korean marketization. The government of Kim Jong Eun has set up policies and systems regarding the spread of marketization, such as guaranteeing individuals a right to dispose certain products on their own and establishing a special economic zone to attract foreign investments. In the forestry sector, the North Korean government has been trying to fully implement its forest restoration plan by carrying out measures like re-claiming of sloping lands that had been previously used by residents. However, as marketization progresses, it is expected that there lies much difficulty in government-led massive mobilization for forest restoration due to the increase of illegal logging to meet high demand for timber, illegal firewood harvesting, collecting non-timber products for livelihoods and illegal crop cultivation to sell in the market. Therefore, South Korea's support for forest restoration should also consider the recent marketization phenomenon in North Korea. It is necessary to formulate strategic measures such as conducting joint commercialization project on agroforestry management using cooperative farming unit, helping to improve income source from small forest lands, and to activate a comprehensive mountain village special economic zone by utilizing forest business. We do hope that our proposed forest rehabilitation strategy in this paper regarding the changes in North Korea's marketization and forest policy can give a meaningful suggestion on supporting forest restoration in North Korea in an effective way.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.10 no.3
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• pp.171-183
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• 2015

Unlike the over 60 years American history of entrepreneurship education, in spite of its short history Korean entrepreneurship education has been rapidly developing. These rapid growth of background, founding activation of for a creative economy realization of core policy of aggressive entrepreneurship education assistance policy and the new government ministries and agencies of government, and growth and global and recession without employment the can and the like or the importance of all the national foundation through. Moreover, the entrepreneurship education came into universities, can not be considered in isolation now academic part of foundation. This study focuses on the analysis the past history of entrepreneurship education based on a variety of data collection. And the analysis is based on three parts; the status of domestic entrepreneurship education, entrepreneurship courses and entrepreneurship research. The result shows that domestic entrepreneurship education is being developed and spread rapidly. Moreover, the number of entrepreneurship courses are increased exponentially in addition to research activities that can contribute to the development of entrepreneurship are also actively performed. Until 2013 the number of entrepreneurship courses opened in universities is 57 times more in last 21 years, meaning that entrepreneurship education is expanding rapidly. In addition, the 142 various journals in domestic are publishing with a research topic related to entrepreneurship, comparing the 44 US journals researching primarily on entrepreneurship, and we can also expect the future growth of domestic research field. This study gives the implication on the field of entrepreneurship education, entrepreneurship courses and entrepreneurship research based on the analysis of existing studies and related materials for the history of entrepreneurship education. With the result of the analysis this study may contribute to entrepreneurship education and research in Korea, and also give insight for nurturing entrepreneurship culture and spirits.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.7 no.1
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• pp.153-164
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• 2012

Recently, as the one way of open Innovation management, Not a company's sole activity, but technology development and infrastructure buildup between companies for the synergistic effect of technological innovation, and technology transfer commercialization is needed. For a successful technology commercialization, it needs many experts like technology transfer agents, A technology transfer agent can be qualified with finishing its registration education course over 40 hours on the basis of the article 14 of law on the Promotion of Technology Transfer and the article 21 of the enforcement. After the 2010 revision of notice, the incorporated company, the Korea Technology Transfer agents has conducted the registration education. Until now, educations were conducted twice in January 2011 and November 2011. Accordingly, this study is necessary to improve the education service's quality and seek remedy through the in-depth evaluation of the present basic education service's quality. The results are as in the following. First, the service quality affect the satisfaction of the education service's quality positively. It is identified that the details of service quality of flexibility, reliability, responsiveness, conviction, and sympathy influence the education service satisfaction positively. Second, the education service satisfaction affects the attitude positively after completing the course. Third, service quality affects the attitude after completing the education course, Also it is identified that the details of service quality of flexibility, reliability, responsiveness, conviction, and sympathy influence the attitude after education completion. It is expected that this research may be used as the basic information for establishing education improvements and be helpful in establishing the technology transfer agents' registration education strategy. Through a continuous research, it is also expected that technology transfer markets are accelerated and qualified technology transfer agents are trained.

• Investigative Magnetic Resonance Imaging
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• v.3 no.2
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• pp.173-178
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• 1999

Purpose : To evaluate the usefulness of functional MRI (fMRI) of visual cortex in patients with ischemic infarction in the occipital lobe. Materials and Methods : Four patients with the symptoms and signs of visual cortical ischemia were included. Functional MRI was performed by 2D-FLASH technique with the parameter of 90/56msec TR/TE, $40^{\circ}$ flip angle, $240{\times}240{\;}FOV,{\;}64{\times}128$ matrix number, 8.32 seconds acquisition time, 8mm slice thickness. An axial slice including both visual cortices was selected and alternative activation and resting of the visual cortex was performed using red color photostimulator. all patients undertook visual field test, and vascular abnormality was examined by MRA (n=4) and DSA (n=2). fMRI results were compared with the results of a visual field test, conventional MRI and cerebral angiography. Results : On fMRI, decreased activity of the visual cortex was found in the occipital lobe corresponding to stenosis of the posterior cerebral artery or its branch noted on angiogram. However, 2 of 4 patients showed no abnormal findings on conventional MRI. Visual field defect was noted in 3 patients, one and of whom showed no abnormality on conventional MRI and diffusion-weighted image, but revealed decreased activity in the corresponding visual cortex on fMRI. Conclusion : fMRI may be a sensitive method for detection of the status of decreased blood flow or vascular reserve which other methods can not.

• Journal of Life Science
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• v.25 no.9
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• pp.993-999
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• 2015

The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.

• The Korean Journal of Mycology
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• v.6 no.2
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• pp.1-10
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• 1978

In de novo biosynthesis of the extracellulor enzymes-proteinsaes, alpha and gluc-amylases during the synchronized differentiation of Aspergillus niger in submerged culture and surface liquid culture were investigated. Gluc-amylase was synthesized in the stage of presporulation in which phialide formation is involved. Proteinase was synthesized both in the stages of conidiophore formation and presporulation. Alpha-amylase was synthesized during presporulation and sporulation stages, the activity of enzyme lasted for seven days on surface liquid culture. It seemed that the synthesis was occured in de novo partly repressed by the catabolite, and its nature was found to be constitutive since it is produced in non-starch medium. Polyacrylamide gel electrophoresis have shown that presporulating and sporulating body produced diverse types of the proteins whereas the earlier stages of vegetative body showed simpler profiles. The uptake of C-14 uracil into RNA and C-14 glutamate into protein were shown to be vigorous in presporulating body rather than those in sporulating body. Coincidence of alpha-amylase biosynthesis in de novo and sporulation may be significant in the study of differentiation in which gene expression is involved.

• Korean Journal of Cognitive Science
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• v.27 no.2
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• pp.159-219
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• 2016

Mathematical ability is important for academic achievement and technological renovations in the STEM disciplines. This study concentrated on the relationship between neural basis of mathematical cognition and its mechanisms. These cognitive functions include domain specific abilities such as numerical skills and visuospatial abilities, as well as domain general abilities which include language, long term memory, and working memory capacity. Individuals can perform higher cognitive functions such as abstract thinking and reasoning based on these basic cognitive functions. The next topic covered in this study is about individual differences in mathematical abilities. Neural efficiency theory was incorporated in this study to view mathematical talent. According to the theory, a person with mathematical talent uses his or her brain more efficiently than the effortful endeavour of the average human being. Mathematically gifted students show different brain activities when compared to average students. Interhemispheric and intrahemispheric connectivities are enhanced in those students, particularly in the right brain along fronto-parietal longitudinal fasciculus. The third topic deals with growth and development in mathematical capacity. As individuals mature, practice mathematical skills, and gain knowledge, such changes are reflected in cortical activation, which include changes in the activation level, redistribution, and reorganization in the supporting cortex. Among these, reorganization can be related to neural plasticity. Neural plasticity was observed in professional mathematicians and children with mathematical learning disabilities. Last topic is about mathematical creativity viewed from Neural Darwinism. When the brain is faced with a novel problem, it needs to collect all of the necessary concepts(knowledge) from long term memory, make multitudes of connections, and test which ones have the highest probability in helping solve the unusual problem. Having followed the above brain modifying steps, once the brain finally finds the correct response to the novel problem, the final response comes as a form of inspiration. For a novice, the first step of acquisition of knowledge structure is the most important. However, as expertise increases, the latter two stages of making connections and selection become more important.