• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.669-675
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• 1998

Taq DNA polymerase exhibits a sizable drawback compared to the other thermophilic DNA polymerases in that it demonstrates lower proof-reading activity due to the deficiency of 3'-5'exonuclease activity. A study was undertaken to improve the 3'-5' exonuclease activity in the PCR of Taq DNA polymerase. The three-dimensional structural alignment of the polymerase and 3'-5' exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase has just a background level of 3'-5'exonuclease activity. A comparison indicated that the two polymerase domains are very similar in primary and tertiary conformations, even though Taq DNA polymerase carries a much shorter 3'-5'exonuclease domain than that of E. coli DNA polymerase I. Those two polymerase domains were interchanged between Taq DNA polymerase and E. coli DNA polymerase I. The 3'-5' exonuclease domain from E. coli DNA polymerase I was separated and pasted into the polymerase domain of Taq DNA polymerase I, which resulted in a functional fusion-Stoffel fragment. The 3'-5'exonuclease activity of the fusion-Stoffel fragment increased up to 48% of the value of the Klenow fragment, while that of Taq DNA polymerase remained at 6.0% of the Klenow fragment.

• Journal of Biomedical Engineering Research
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• v.26 no.5
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• pp.265-270
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• 2005

A new Computer Integrated Surgical Robot system is composed of a surgical robot, a surgical planning system, and an optical tracking system. The system plays roles of an assisting surgeon and taking the place of surgeons for inserting a pedicle screw in spinal fusion. Compared to pure surgical navigation systems as well as conventional methods for spinal fusion, it is able to achieve better accuracy through compensating for the portending movement of the surgical target area. Furthermore, the robot can position and guide needles, drills, and other surgical instruments or conducts drilling/screwing directly. Preoperatively, the desired entry point, orientation, and depth of surgical tools for pedicle screw insertion are determined by the surgical planning system based on CT/MR images. Intra-operatively, position information on surgical instruments and targeted surgical areas is obtained from the navigation system. Two exemplary experiments employing the developed image-guided surgical robot system are conducted.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.207-216
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• 2018

Solanum berthaultii is one of the wild diploid Solanum species, which is an excellent resource in potato breeding owing to its resistance to several important pathogens. On the other hand, sexual hybridization between S. berthaultii and S. tuberosum (potato) is limited because of their sexual incompatibility. Therefore, cell fusion can be used to introgress various novel traits from this wild species into the cultivated potatoes. After cell fusion, it is crucial to identify fusion products with the aid of molecular markers. In this study, the chloroplast genome sequence of S. berthaultii obtained by next-generation sequencing technology was described and compared with those of five other Solanum species to develop S. berthaultii specific markers. A total sequence length of the chloroplast genome is 155,533 bp. The structural organization of the chloroplast genome is similar to those of the five other Solanum species. Phylogenic analysis with 25 other Solanaceae species revealed that S. berthaultii is most closely located with S. tuberosum. Additional comparison of the chloroplast genome sequence with those of the five Solanum species revealed 25 SNPs specific to S. berthaultii. Based on these SNPs, six PCR-based markers for differentiating S. berthaultii from other Solanum species were developed. These markers will facilitate the selection of fusion products and accelerate potato breeding using S. berthaultii.

• Bulletin of the Korean Chemical Society
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• v.32 no.7
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• pp.2187-2192
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• 2011

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

• Molecular & Cellular Toxicology
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• v.3 no.3
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• pp.159-164
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• 2007

Stimulation of epidermal growth factor receptor (EGFR) is essential in signaling pathway of tumor cells. Thus, EGFR has intensely studied as an anticancer target. We developed hologram quantitative structure activity relationship (HQSAR) models for data set which consists of tricyclic azepine derivatives showing inhibitory activities for EGFR. The optimal HQSAR model was generated with fragment size of 6 to 7 while differentiating fragments having different atom and connectivity. The model showed cross-validated $q^2$ value of 0.61 and non-cross-validated $r^2$ value of 0.93. When the model was validated with an external set excluding one outlier, it gave predictive $r^2$ value of 0.43. The contribution maps generated from this model were used to interpret the atomic contribution of each atom to the overall inhibition activity. This can be used to find more efficient EGFR inhibitors.

• Progress in Superconductivity
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• v.9 no.2
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• pp.152-156
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• 2008

Detailed field and strain dependence of the critical current and the n-value for an internal-tin processed $Nb_3Sn$ strand have been measured. Both the compressive and tensile strain is applied reversibly using Walter spiral probe made of BeCu up to 0.73 %. There is a correlation between the critical current and the n-value for the $Nb_3Sn$ strand studied in this work and the field dependence of the n-value is in agreement with a recent empirical formula. It was further shown that the critical current can be reasonably well fitted by the scaling law based on strong-coupling theory of superconductivity using the relation between the critical current and the n-value.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.6
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• pp.1023-1041
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• 2010

In cognitive radio networks, cooperative spectrum sensing schemes are proposed to improve the performance of detecting licensees by secondary users. Commonly, the cooperative sensing can be realized by means of hard decision fusion (HDF) or soft decision fusion (SDF) schemes. The SDF schemes are superior to the HDF ones in terms of the detection performance whereas the HDF schemes are outperforming the SDF ones when the traffic overhead is taken into account. In this paper, a hybrid SFD-HDF cluster-based approach is developed to jointly exploit the advantages of SFD and HDF schemes. Different SDF schemes have been proposed and compared within a given cluster whereas the OR-rule base HDF scheme is applied to combine the decisions reported by cluster headers to a common receiver or base station. The computer simulations show promising results as the performance of the proposed scenario of hybridizing soft and hard fusion schemes is significantly outperforming other different combinations of conventional SDF and HDF schemes while it noticeably reduces the network traffic overhead.

• Journal of Microbiology
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• v.35 no.1
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• pp.72-77
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• 1997

The baculovirus gp64 glycoprotein is a major component of the envelope protein of budded virus (BV). It has been shown that the gp64 glycoprotein plays an essential role in the infection process, especialy fusion between virus envelope and cellular endosomic membrane. Recently we reported optimal conditions required for gp64-mediated membrane fusion in pGP64 DNA transfected Spodoptera frugiperda (Sf9) cells (H. J. Kim and J. M. Yang, Jour, Microbiology, 34.7-14). In order to investigate the role of hydrophobicity within the fusion domain of the gp64 glycoprotein for membrane fusion, 13 mutants which have substitution mutation within hydrophobic region I were constructed by PCR-derived site-derected mutagenesis. Each mutated gp64 glycoproteins was transiently expressed by transfecting plasmid DNA into Spodoptera frugiperda (Sf9) cells. Oligomerization of the transisently expressed gp64 glycoproteins was a nalysed by running them on SDS-polyacrylamide gel electrophoresis under non-reducing condition followed by immunoblotting. All of the mutant gp64 glycoproteins expect cysteine-228 were able to form trimers. These results suggest that hydrophobic region I of the gp64 may not be responsible for the oligomerization of the gp64 glycoprotein.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.4 no.1
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• pp.165-177
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• 2000

A fuzzy controller need membership functions and the control rules depend on heuristic knowledge of expertises entirely. On account of, it is possible that a desired performance of a fuzzy controller can not be guaranteed or easily degraded under some circumstances such as a change of plant parameter which exporters do not considered. Therefore, in this paper we tried to increase the controller's efficiency by adjusting the control rules and the parameters of the membership functions by using a genetic algorithm. We also proposed the Self-Organizing Fuzzy Controller which uses the Fusion Method in order to minimize the number of control rules and to construct the intuitive controller. For validation of the proposed algorithm, we design the Autonomous Guided Vehicle Controller, then apply to variant condition.

• Journal of Korean Society for Geospatial Information Science
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• v.10 no.5 s.23
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• pp.53-65
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• 2002

The most widespread techniques for DEM generation are stereoscopy for optical sensor images and SAR interferometry(InSAR) for SAR images. These techniques suffer from certain sensor and processing limitations, which can be overcome by the synergetic use of both sensors and DEMs respectively. This study is associated with improvements of accuracy with consistency of image's characteristics between two different DEMs coming from stereoscopy for the optical images and interferometry for SAR images. The MWD(Multiresolution Wavelet Decomposition) and HPF(High-Pass Filtering), which take advantage of the complementary properties of SAR and stereo optical DEMs, will be applied for the fusion process. DEM fusion is tested with two sets of SPOT and ERS-l/-2 satellite imagery and for the analysis of results, DEM generated from digital topographic map(1 to 5000) is used. As a result of an integration of DEMs, it can more clearly portray topographic slopes and tilts when applying the strengths of DEM of SAR image to DEM of an optical satellite image and in the case of HPF, the resulting DEM.