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Graft-taking and Growth Characteristics of Grafted Cucumber(Cucumis sativus L.) Seedlings as Affected by Light Quality and Blink Cycle of LED Modules (LED 모듈의 광질 및 점멸주기에 따른 오이접목묘의 활착 및 생장 특성)

  • Kim, Hyeong Gon;Choi, Yu Hwa;Kim, Yong Hyeon
    • Journal of Bio-Environment Control
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    • v.28 no.2
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    • pp.143-149
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    • 2019
  • This study was conducted to investigate the graft-taking and growth of grafted cucumber seedlings as affected by light quality and blink cycle of LED modules. Four light quality treatments, namely blue, red, blue+red, white LED and four blink cycle levels of 5s/5s, 7s/3s, 9s/1s and control were provided to investigate the effect of lighting quality and blink cycle on the graft-taking and growth of grafted cucumber seedlings. Photoperiod for the control was 12/12 h. Photosynthetic photon flux, air temperature, and relative humidity for healing were maintained at $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $25^{\circ}C$, and 90%, respectively. There was no significant difference in graft-taking of grafted cucumber seedlings according to light quality except the blue LED with the blink cycle of 5s/5s. Regardless of the blink cycle, there was no significant difference in graft-taking of cucumber seedlings healed under red, blue+red, and white LED modules. These results implied that the effects of light quality and blink cycle on the graft-taking were not significant. Differences in the leaf length, leaf area, and fresh weight of cucumber seedlings healed blue or red LED with the blink cycle of 9s/1s were found to be significant. There was no significant effect of the blink cycle on the growth of cucumber seedlings healed under white LED modules. The prices of white LED are gradually falling due to increased demand. Considering the manufacturing unit price of white LED modules, the cost savings of 10-15% are expected as compared to the conventional blue/red LED modules. Therefore, it was concluded that the use of white LED modules will be economical as an artificial lighting sources for healing of grafted seedlings.

Automatic Fracture Detection in CT Scan Images of Rocks Using Modified Faster R-CNN Deep-Learning Algorithm with Rotated Bounding Box (회전 경계박스 기능의 변형 FASTER R-CNN 딥러닝 알고리즘을 이용한 암석 CT 영상 내 자동 균열 탐지)

  • Pham, Chuyen;Zhuang, Li;Yeom, Sun;Shin, Hyu-Soung
    • Tunnel and Underground Space
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    • v.31 no.5
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    • pp.374-384
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    • 2021
  • In this study, we propose a new approach for automatic fracture detection in CT scan images of rock specimens. This approach is built on top of two-stage object detection deep learning algorithm called Faster R-CNN with a major modification of using rotated bounding box. The use of rotated bounding box plays a key role in the future work to overcome several inherent difficulties of fracture segmentation relating to the heterogeneity of uninterested background (i.e., minerals) and the variation in size and shape of fracture. Comparing to the commonly used bounding box (i.e., axis-align bounding box), rotated bounding box shows a greater adaptability to fit with the elongated shape of fracture, such that minimizing the ratio of background within the bounding box. Besides, an additional benefit of rotated bounding box is that it can provide relative information on the orientation and length of fracture without the further segmentation and measurement step. To validate the applicability of the proposed approach, we train and test our approach with a number of CT image sets of fractured granite specimens with highly heterogeneous background and other rocks such as sandstone and shale. The result demonstrates that our approach can lead to the encouraging results on fracture detection with the mean average precision (mAP) up to 0.89 and also outperform the conventional approach in terms of background-to-object ratio within the bounding box.

Annual Analysis of the Agronomic Traits of Global Wheat Germplasms in the Korean Environment (국내환경에서 밀 유전자원의 연차간 농업특성 분석)

  • Son, Jae-Han;Yang, Jinwoo;Kang, Chon-Sik;Kim, Kyeong-Hoon;Kim, Kyeong-Min;Jeong, Han-Yong;Park, Jinhee;Son, Ji-Young;Park, Tae-il;Choi, Changhyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.2
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    • pp.120-129
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    • 2021
  • Securing a range of wheat resources is of particular importance with respect to wheat breeding, as it provides a broad genetic foundation. Although wheat breeders have used different wheat germplasms as material resources in current breeding systems, the traits of most germplasms collected from foreign countries differ from the unique traits that have evolved in the Korean environment. In this study, conducted over a 2-year period (2018 and 2019), we therefore evaluated the agricultural traits 1,967 wheat germplasms collected not only in Korea but also worldwide. During the period from sowing to February, the average temperature in 2019 was greater than 1℃, whereas from March to June, the average temperature was approximately 0.9℃ higher in 2018. Compared with the growth recorded in 2018, the stem length in 2019 increased by approximately 20 cm in 2019, and there were notable differences heading date and maturation between 2018 and 2019. In 2019, the heading dates of 973 and 713 wheat resources were earlier and later than those in 2018, respectively. Moreover, stem length was found to be highly correlated with the heading date and maturation. In Korea, where the rainy season and tine of rice transplantation overlap with the time of wheat harvest, early flowering time with high grain yield has been the most important selection target with respect to wheat breeding. We anticipate that the findings of this study will provide would use a foundation for the selection of elite materials and the development of resource core-sets for Korean wheat breeding programs.

Inhibitory Effects of Prunus mume Solvent Fractions on Human Colon Cancer Cells (매실 분획물에 의한 인체 대장암세포 억제 효과)

  • Kim, Jeong-Ho;Cho, Hyun-Dong;Won, Yeong-Seon;Heo, Ji-An;Kim, Ji-Young;Kim, Hwi-Gon;Han, Sim-Hee;Moon, Kwang-Deog;Seo, Kwon-Il
    • Journal of Life Science
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    • v.29 no.11
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    • pp.1227-1234
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    • 2019
  • Prunus mume, also known as maesil, is a popular fruit consumed in East Asia (Korea, Japan, and China). It contains high amounts of organic acids, minerals, and polyphenols and has been used as a medication for fever, vomiting, and detoxification. In this study, the anti-proliferative and apoptotic effects of solvent fractions from maesil were evaluated using sulforhodamine B (SRB) assays, morphological evaluations, Hoechst 33258 staining, and western blotting. Addition of the maesil methanol fraction (MMF) and the maesil butanol fraction (MBF) significantly and dose-dependently decreased the cell viability of HT-29 human colon cancer cells. Colony-forming assays confirmed that the MMF and MBF treatments decreased colony numbers when compared with untreated control cells. Treatment of HT-29 cells with MMF and MBF caused a distortion of the cell morphology to a shrunken cell mass. Treatment with MMF and MBF also dose-dependently increased nuclear condensation and the formation of apoptotic bodies in HT-29 cells. Treatment with MMF and MBF significantly and dosedependently increased the expression of Bax (a pro-apoptotic protein), caspase-3, and poly ADP-ribose polymerase (PARP) and decreased the expression of Bcl-2 (an anti-apoptotic protein). MMF significantly and dose-dependently inhibited cell proliferation induced by bisphenol A, an environmental hormone. Therefore, MMF may have potential use as a functional food and as a possible therapeutic agent for the prevention of colon cancer.

Determination of Proper Irrigation Scheduling for Automated Irrigation System based on Substrate Capacitance Measurement Device in Tomato Rockwool Hydroponics (토마토 암면재배에서 정전용량 측정장치를 기반으로 한 급액방법 구명)

  • Han, Dongsup;Baek, Jeonghyeon;Park, Juseong;Shin, Wonkyo;Cho, Ilhwan;Choi, Eunyoung
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.366-375
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    • 2019
  • This experiment aims to determine the proper irrigation scheduling based on a whole-substrate capacitance using a newly developed device (SCMD) by comparing with the integrated solar radiation automated irrigation system (ISR) and sap flow sensor automated irrigation system (SF) for the cultivation of tomato (Solanum lycopersicum L. 'Hoyong' 'Super Doterang') during spring to winter season. For the SCMD system, irrigation was conducted every 10 minutes after the first irrigation was started until the first run-off was occurred, of which the substrate capacitance was considered to be 100%. When the capacitance threshold (CT) was reached to the target point, irrigation was re-conducted. After that, when the target drain volume (TDV) was occurred, the irrigation stopped. The irrigation volume per event for the SCMD was set to 50, 75, or 100 mL at CT 0.9 and TDV 100 mL during the spring to summer cultivation, and the CT was set to 0.65, 0.75, 0.80, or 0.90 in the winter cultivation. When the irrigation volume per event was set to 50, 75, or 100 mL, the irrigation frequency in a day was 39, 29, and 19, respectively, and the drain rate was 3.04, 9.25, and 20.18%, respectively. When the CT was set to 0.65, 0.75, or 0.90 in winter, the irrigation frequency was about 6, 7, 15 times, respectively and the drain rate was 9.9, 10.8, 35.3% respectively. The signal of stem sap flow at the beginning of irrigation starting time did not correspond to that of solar irradiance when the irrigation volume per event was set to 50 or 75 mL, compared to that of 100 mL. In winter cultivation, the stem sap flow rate and substrate volumetric water content at the CT 0.65 treatment were very low, while they were very high at CT 0.90 was high. All the integrated data suggest that the proper range of irrigation volume per event is from 75 to 100 mL under at CT 0.9 and TDV 100 mL during the spring to summer cultivation, and the proper CT seems to be higher than 0.75 and lower than 0.90 under at 75 mL of the irrigation volume per event and TDV 70 mL during the winter cultivation. It is going to be necessary to investigate the relationship between capacitance value and substrate volumetric water content by determining the correction coefficient.

The Characteristics of Dolmen Culture and Related Patterns during the End Phase in the Gyeongju Region (경주 지역 지석묘 문화의 특징과 종말기의 양상)

  • Lee, Soohong
    • Korean Journal of Heritage: History & Science
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    • v.53 no.4
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    • pp.216-233
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    • 2020
  • This study set out to review tomb culture in the Gyeongju region during the Bronze Age, and also examine the patterns of dolmens during their end phase. For these purposes, the study analyzed 18 tomb relics from the Bronze Age and nine from the early Iron Age. Gyeongju belongs to the Geomdan-ri cultural zone. Approximately 120 tombs from the Bronze Age have been excavated in the Gyeongju region. There are fewer tombs than dwellings in the region, which is a general characteristic of the Geomdan-ri cultural zone. Although the number of tombs is small, the detailed structure of the dead body is varied. During the Bronze Age, tombs in the Gyeongju region were characterized by more prolific construction of pit tombs, dolmens with boundaries, and stacked stone altars than were the cases in other areas. There is a great possibility that the pit tombs in the Gyeongju region were influenced by their counterparts in the northeastern parts of North Korea, given the spindle whorl artifacts buried at the Dongsan-ri sites. Dolmens with boundaries and stacked stone altars are usually distributed in the Songguk-ri cultural zone, and it is peculiar that instances of these are found in large numbers in the Gyeongju region as part of the Geomdanri cultural zone. Even in the early Iron Age, the building of dolmens with boundaries and stacked stone altars continued in the Gyeongju region under the influence of the Bronze Age. A new group of people moved into the area, and they crafted ring-rimmed pottery and built wooden coffin tombs. In the early Iron Age, new rituals performed in high places also appeared, and were likely to provide venues for memorial services for heavenly gods in town-center areas. The Hwacheon-ri Mt. 251-1 relic and the Jukdong-ri relic are ruins that exhibit the aspect of rituals performed in high places well. In these rituals performed in high places, a stacked stone altar was built with the same form as the dolmens with boundaries, and a similar rock to the cover stone of a dolmen was used. People continued to build and use dolmens with boundaries and stacked stone altars while sustaining the Bronze Age traditions, even into the early Iron Age, because the authority of dolmens was maintained. Some dolmens with boundaries and stacked stone altars, known as being Bronze Age in origin, would have continued to be used in ritual practices until the early Iron Age. Entering the latter half of the second century B.C., wooden coffin tombs began to propagate. This was the time when the southern provinces, including the Gyeongju region, were included in the East Asian network, with the spread of ironware culture and the arrival of artifacts from central China. Around this time, dolmen culture faded into history with a new era beginning in its place.

Effects of Initial Shoot, Root Length, and Acclimating Substrates on Survival Rate of Plantlets Regenerated from Somatic Embryos of Larix kaempferi (일본잎갈나무 체세포배 유래 식물체의 초기 신초와 뿌리 길이, 순화용 기질이 생존율에 미치는 영향)

  • Lee, Na Nyum;Yun, A Young;Kim, Ji Ah;Kim, Tae Dong;Kim, Yong Wook;Han, Sim Hee
    • Journal of Korean Society of Forest Science
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    • v.109 no.4
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    • pp.413-420
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    • 2020
  • We analyzed the growth characteristics of each cell line and acclimating substrate of Larix kaempferi plantlets regenerated from somatic embryos, with the goal of increasing the survival rate during the acclimation phase. Somatic embryos from three embryogenic cell lines (L14-66, L16-18, and L17-B4) were used, and the acclimating substrates were commercial soils for Larix species (Larix-Soil) and horticultural corps (Hort-Soil), Elle-pot, and Peat-plug. The average initial shoot and root length was shortest in L14-66 and longest in L17-B4. The average survival rate by cell line was highest (87.0%) in L17-B4 and lowest (64.3%) for L14-66. Survival rates by substrate were highest in Elle-pot (88.5%) and Peat-plug (88.9%). The initial shoot length of the L14-66 plantlets was highly correlated with survival rates in the Larix-Soil (r = 0.852), Hort-Soil (r = 0.692), and Elle-pot (r = 0.867) substrates, but not in Peat-plug with high total nitrogen content. The initial shoot length of the L17-B4 plantlets was not correlated with the survival rate in any of the substrates. The initial root length of the L14-66 plantlets was highly related to survival rates in the Larix-Soil (r = 0.986), Elle-pot (r = 0.846), and Peat-plug (r = 0.802) substrates, and the survival rate of the plantlets was higher as the initial root length was longer. The initial root length of the L17-B4 plantlets was related to survival rate only in the Larix-Soil (r = 0.896) and Elle-pot (r = 0.696) substrates. In conclusion, to increase the survival rate of plantlets, root length should be considered over shoot length, and it is recommended to use substrates with high nitrogen content, such as Peat-plug, or to add fertilizer, during the acclimating process. In addition, in order to increase the survival rate, lines with high initial growth should be developed.

Factors related to COVID-19 Incidence and Mortality rate in Gyeongsangbuk-do, Korea (경상북도 지역의 코로나19 발생률 및 사망률 관련요인)

  • Kim, Dong-Hwi;Park, Sung-Jun;Kang, Hyun-Jun;Yeom, Eun-Jung;Yoo, Na-Eun;Lee, Jeong-Min;Nam, Eun-Ha;Park, Ji-Hyuk;Lee, Kwan
    • Journal of agricultural medicine and community health
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    • v.45 no.4
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    • pp.235-244
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    • 2020
  • Objectives: Gyeongsangbuk-do has entered a super-aged society with 20.7% of the population aged 65 and older. As of April 30, 2020, the death rate of COVID-19(3.8 people) in Gyeongsangbuk-do is higher than the national mortality rate (2.3 people), and the fatality rate of COVID-19 by age accounts for more than half of the total of 58.6%, so it is time to propose to prevent infectious diseases in the event of additional infectious disease disasters COVID-19. Methods: We collected daily data on the number of confirmed cases and deaths due to COVID-19 from 19 February to 30 April 2020. The data collected was evaluated using the SPSS 21.0 statistical package. Results: As a result of comparing the incidence and death-related factors of confirmed patients in Gyeongsangbuk-do, there were significant differences in age group (p<0.001), underlying disease (p<0.001), and residence type (p<0.033). Conclusion: Factors affecting the mortality rate of confirmed patients in Gyeongsangbuk-do have been combined with individual level factors(age, gender, underlying disease), which means individual characteristics that have existed since before the disease, and regional level factors(Type of Residence), which are external factors that enable the use of medical resources. Therefore, each local government is required to establish preventive measures considering individual and regional level factors.

Very short-term rainfall prediction based on radar image learning using deep neural network (심층신경망을 이용한 레이더 영상 학습 기반 초단시간 강우예측)

  • Yoon, Seongsim;Park, Heeseong;Shin, Hongjoon
    • Journal of Korea Water Resources Association
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    • v.53 no.12
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    • pp.1159-1172
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    • 2020
  • This study applied deep convolution neural network based on U-Net and SegNet using long period weather radar data to very short-term rainfall prediction. And the results were compared and evaluated with the translation model. For training and validation of deep neural network, Mt. Gwanak and Mt. Gwangdeoksan radar data were collected from 2010 to 2016 and converted to a gray-scale image file in an HDF5 format with a 1km spatial resolution. The deep neural network model was trained to predict precipitation after 10 minutes by using the four consecutive radar image data, and the recursive method of repeating forecasts was applied to carry out lead time 60 minutes with the pretrained deep neural network model. To evaluate the performance of deep neural network prediction model, 24 rain cases in 2017 were forecast for rainfall up to 60 minutes in advance. As a result of evaluating the predicted performance by calculating the mean absolute error (MAE) and critical success index (CSI) at the threshold of 0.1, 1, and 5 mm/hr, the deep neural network model showed better performance in the case of rainfall threshold of 0.1, 1 mm/hr in terms of MAE, and showed better performance than the translation model for lead time 50 minutes in terms of CSI. In particular, although the deep neural network prediction model performed generally better than the translation model for weak rainfall of 5 mm/hr or less, the deep neural network prediction model had limitations in predicting distinct precipitation characteristics of high intensity as a result of the evaluation of threshold of 5 mm/hr. The longer lead time, the spatial smoothness increase with lead time thereby reducing the accuracy of rainfall prediction The translation model turned out to be superior in predicting the exceedance of higher intensity thresholds (> 5 mm/hr) because it preserves distinct precipitation characteristics, but the rainfall position tends to shift incorrectly. This study are expected to be helpful for the improvement of radar rainfall prediction model using deep neural networks in the future. In addition, the massive weather radar data established in this study will be provided through open repositories for future use in subsequent studies.

Analytical method study for cephalexin with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) applicable for residue studies in the whiteleg shrimp Litopenaeus vannamei (흰다리새우(Litopenaeus vannamei)에서 cephalexin의 잔류농도 연구를 위한 LC-MS/MS 분석법 연구)

  • Yang, Chan Yeong;Bae, Jun Sung;Lee, Chae Won;Jeong, Eun Ha;Lee, Ji-Hoon;Bak, Su-Jin;Choi, Sang-Hoon;Park, Kwan Ha
    • Journal of fish pathology
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    • v.34 no.1
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    • pp.71-80
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    • 2021
  • Cephalexin, a semi-synthetic cephalosporin antibiotic, has long been used in fish aquaculture in various countries under legal authorization. The drug is thus widely available for use in other aquatic species except fishes like the crustacean whiteleg shrimp. This study aims to develop a sensitive method for laboratory residue studies to adopt in withdrawal period determinations. Through repeated trials from the existing methods developed for other food animal tissues, it was possible to achieve a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The results showed that at a concentration of 0.1 mg/kg, the recovery rate was 81.79%, and C.V. value was 8.2%, which meet the recovery rate and C.V. recommended by Codex guideline. After satisfactory validation of analytical procedures, applicability to the shrimp tissue was confirmed in experimentally cephalexin-treated whiteleg shrimp. As a result, most muscle samples were detected below the limit of quantification (0.05 mg/kg) after day 3, and most hepatopancreas samples were detected below the limit of quantification after day 14. In particular, the limit of quantification 0.05 ppm with the presently developed method suggests sufficient sensitive over the current legal maximum residue limit of 0.2 mg/kg set for fishes.