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Effect of Antibody Titer on Xenograft Survival in Pig-To-Dog Heterotopic Cardiac Xenotransplantation -Opening of Xenotrasplantation Era- (돼지\longrightarrow개 이소이종심장 이식모델에시 생존에 미치는 항체 역가의 영향 -이종이식시대의 개막-)

  • 이정렬;김희경;김지연;최대영;이재형;위현초;강희정;김영태;강병철
    • Journal of Chest Surgery
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    • v.37 no.5
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    • pp.391-400
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    • 2004
  • Xenotransplantation in discordant species results in immediate and irreversible hyperacute rejection due to natural antibodies, IgM. With this, antibody depletion is one option to reduce hyperacute rejection, we investigated the effect of PCPP (postcentrifugal plasmapheresis) on the depletion of natural antibodies and the effect of antibody titer on xenograft survival. Material and Method: Outbred swines (n=4) weighing 10∼20 kg were used as donors and mongrel dogs (n=4) weighing 25∼30 kg were used as recipients. Recipient canines underwent plasmapheresis (COBE TPE Laboratories, Lakewood. CO, USA). Pre-transplantation PCPP was peformed on day -2 and day 0. There were three groups (Group 0: no PCPP, Group 1: 1 pla sma-volume (PV) at day -2 and 2 PV at day 0, Group 2: 2 PV at day -2 and 2 PV at day 0). A swine heart was heterotopically transplanted into a recipient's abdominal infrarenal aorta and inferior vena cava. Mean percent depletion of total IgM and IgG in plasma of the recipients was calculated. Serum albumin, electrolyte, complement activity and coagulation factors were measured. Histopathologic examination of heart specimens was performed. Result: Mean percent depletion of IgM and IgG were 95.7$\pm$1.2%, 80.5$\pm$2.4% in the group 2 at the end of PCPP. The percent depletion of serum albumin concentration was decreased from 2.8 to 1.4 g/㎗ in the group 1 and 3.0 to 1.5 g/㎗ in the group 2. Complement hemolytic activity was decreased in group 1 and 2, but returned to normal level within 24 hours. Complement hemolytic activity was reduced to 10% of pre-PCPP level in group 2. Serum fibrinogen decreased to 20% or less and was recovered within 24 hours in group 2. Antithrombin III decreased but less than fibrinogen. PT and aPTT were sometimes but not always prolonged during plasmapheresis. After plasmapheresis, PT and aPTT were prolonged beyond the measurable level. D-dimer was not found during PCPP, but appeared and maintained from 10 minutes after trasplantation. Graft Survival time was 5 min in group 0, and it was 90$\pm$0 min in the group 2. Histopathologic changes were more typically characterized by edema, hemorrhages, thrombosis in all groups at the end of experiment. Conclusion: PCPP effectively removed immuoglobulins and reduced the titer of natural antibodies, as a result, significantly prololonged swine heart xenograft survival.

A Study on the Water Quality Management of Modular Revetment Structure installed Low-Maintenance Constructed Wetland (플랜터형 호안구조물을 설치한 저관리형 습지의 수질 제어 기작에 관한 연구)

  • Ham, Eun Kyung;Choi, Yun Eui;Kim, Min;Chon, Jinhyung
    • Journal of Wetlands Research
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    • v.17 no.2
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    • pp.184-192
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    • 2015
  • This study is a basic research for low maintenance constructed wetlands which can prevent water quality deterioration due to wetland soils. The purpose of this study is to analyze water quality control mechanisms of constructed wetland on the artificial ground which installed the modular revetment structure(MRS), a device that separates water and soil. This study was then conducted with two different wetland mesocosms (a treatment plot and a control). These mesocosms were monitored to analyze effects of water quality control of the MRS. A treatment plot was built, and separated into soil and water, by filling the MRS with the decomposed granite soil in the mesocosm made of rubber material. A control plot was built where the decomposed granite soils were exposed to water by leaving the soil on the bottom of the mesocosm made of rubber material. Water quality was then analyzed by using Kolmogorov-Smirnov Z examination which then showed that pH, BOD, SS, Chl-a, T-P, T-N had statistically valid difference between a container with the MRS and one without it. According to the analysis of the water quality, the temperature and the EC level came out similarly and both mesocosm showed same level on pH and DO. A treatment plot had higher levels of BOD, SS Chl-a, and T-P. A control plot's T-N value was little lower than that of the treatment plot. This study suggests method of constructed wetlands using the MRS prevents problems occurring in wetland soils: aridity of wetlands by soil erosion, eutrophication, and algal blooms due to nutrients released from wetland soil. These functions of constructed wetland with the MRS increase aesthetic, ecologic, social, and economic value of the wetland. Outcomes of this research will later enable more proficient way of stabilizing water quality and provide data for planning low maintenance constructed wetlands.

DIFFERENCE IN BOND STRENGTH ACCORDING TO FILLING TECHNIQUES AND CAVITY WALLS IN BOX-TYPE OCCLUSAL COMPOSITE RESIN RESTORATION (박스 형태의 복합레진 수복시 충전법 및 와동벽에 따른 결합력 차이에 관한 연구)

  • Ko, Eun-Joo;Shin, Dong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.34 no.4
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    • pp.350-355
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    • 2009
  • Bond strength depends on characteristics of bonding surface and restorative technique. The majority of studies dealing with dentin bond strength were carried out on flat bonding surface, therefore, difference of bond strength between axial wall and pulpal wall is not clear yet. This study evaluated bonding difference between cavity walls in class I composite resin restoration with different filling techniques. Twenty extracted caries-free human third molars were used. Cavities were prepared in 6 ${\times}$4 ${\times}$3 mm box-type and divided into four groups according to filling technique and bonding surface: Group I; bulk filling - pulpal wall, Group II; bulk filling - axial wall, Group III; incremental filling - pulpal wall, Group IV; incremental filling - axial wall. Cavities were filled with Filtek $Z250^{(R)}$(3M/ESPE., USA) and Clearfill SE $bond^{(R)}$(Kuraray, Japan). After 24 hour-storage in $37^{\circ}C$water, the resin bonded teeth were sectioned bucco-lingualy at the center of cavity. Specimens were vertically sectioned into 1.0 ${\times}$1.0 mm thick serial sticks perpendicular to the bond surface using a low-speed diamond saw (Accutom 50, Struers, Copenhagen, Denmark) under water cooling. The trimmed specimens were then attached to the testing device and in turn, was placed in a universal testing machine (EZ test, Shimadzu Co., Kyoto, Japan) for micro-tensile testing at a cross-head speed of 1 mm/min. The results obtained were statistically analyzed using 2-way ANOVA and t-test at a significance level of 95%. The results were as follows: 1. There was no significant difference between bulk filling and incremental filling. 2. There was no significant difference between pulpal wall and axial wall, either. Within the limit of this study, it was concluded that microtensile bond strength was not affected by the filling technique and the site of cavity walls.

Analysis of Optimal Pathways for Terrestrial LiDAR Scanning for the Establishment of Digital Inventory of Forest Resources (디지털 산림자원정보 구축을 위한 최적의 지상LiDAR 스캔 경로 분석)

  • Ko, Chi-Ung;Yim, Jong-Su;Kim, Dong-Geun;Kang, Jin-Taek
    • Korean Journal of Remote Sensing
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    • v.37 no.2
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    • pp.245-256
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    • 2021
  • This study was conducted to identify the applicability of a LiDAR sensor to forest resources inventories by comparing data on a tree's position, height, and DBH obtained by the sensor with those by existing forest inventory methods, for the tree species of Criptomeria japonica in Jeolmul forest in Jeju, South Korea. To this end, a backpack personal LiDAR (Greenvalley International, Model D50) was employed. To facilitate the process of the data collection, patterns of collecting the data by the sensor were divided into seven ones, considering the density of sample plots and the work efficiency. Then, the accuracy of estimating the variables of each tree was assessed. The amount of time spent on acquiring and processing the data by each method was compared to evaluate the efficiency. The findings showed that the rate of detecting standing trees by the LiDAR was 100%. Also, the high statistical accuracy was observed in both Pattern 5 (DBH: RMSE 1.07 cm, Bias -0.79 cm, Height: RMSE 0.95 m, Bias -3.2 m), and Pattern 7 (DBH: RMSE 1.18 cm, Bias -0.82 cm, Height: RMSE 1.13 m, Bias -2.62 m), compared to the results drawn in the typical inventory manner. Concerning the time issue, 115 to 135 minutes per 1ha were taken to process the data by utilizing the LiDAR, while 375 to 1,115 spent in the existing way, proving the higher efficiency of the device. It can thus be concluded that using a backpack personal LiDAR helps increase efficiency in conducting a forest resources inventory in an planted coniferous forest with understory vegetation, implying a need for further research in a variety of forests.

A Study on the Fabrication and Comparison of the Phantom for CT Dose Measurements Using 3D Printer (3D프린터를 이용한 CT 선량측정 팬텀 제작 및 비교에 관한 연구)

  • Yoon, Myeong-Seong;Kang, Seong-Hyeon;Hong, Soon-Min;Lee, Youngjin;Han, Dong-Koon
    • Journal of the Korean Society of Radiology
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    • v.12 no.6
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    • pp.737-743
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    • 2018
  • Patient exposure dose exposure test, which is one of the items of accuracy control of Computed Tomography, conducts measurements every year based on the installation and operation of special medical equipment under Article 38 of the Medical Law, And keep records. The CT-Dose phantom used for dosimetry can accurately measure doses, but has the disadvantage of high price. Therefore, through this research, the existing CT - Dose phantom was similarly manufactured with a 3D printer and compared with the existing phantom to examine the usefulness. In order to produce the same phantom as the conventional CT-Dose phantom, a 3D printer of the FFF method is used by using a PLA filament, and in order to calculate the CTDIw value, Ion chambers were inserted into the central part and the central part, and measurements were made ten times each. Measurement results The CT-Dose phantom was measured at $30.44{\pm}0.31mGy$ in the periphery, $29.55{\pm}0.34mGy$ CTDIw value was measured at $30.14{\pm}0.30mGy$ in the center, and the phantom fabricated using the 3D printer was measured at the periphery $30.59{\pm}0.18mGy$, the central part was $29.01{\pm}0.04mGy$, and the CTDIw value was measured at $30.06{\pm}0.13mGy$. Analysis using the Mann - Whiteney U-test of the SPSS statistical program showed that there was a statistically significant difference in the result values in the central part, but statistically significant differences were observed between the peripheral part and CTDIw results I did not show. In conclusion, even in the CT-Dose phantom made with a 3D printer, we showed dose measurement performance like existing CT-Dose phantom and confirmed the possibility of low-cost phantom production using 3D printer through this research did it.

A Study on the Difference of Scattered Rays with or Without Gonadal Shielding During Chest Computed Tomography (흉부 전산화 단층 촬영 검사 시 발생하는 생식선 차폐 유무에 따른 산란 선량 차이에 관한 연구)

  • Kwak, Jong Hyeok;Kim, Gyeong Rip;Sung, Hyun Chul;Kim, Seung Won;Song, Geun Sung;Choi, Min Gyeong;Lee, Sang Weon
    • Journal of the Korean Society of Radiology
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    • v.15 no.2
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    • pp.109-115
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    • 2021
  • This study is a study on the difference in dose according to the presence or absence of gonadal shielding of scattered rays generated during chest computed tomography examination, and the scattered dose of the examination site was measured by placing the RadEye G-10 device in the center of the phantom. When the gonads are not shielded, the scattering lines of the whole, both sides, posterior and gonads are measured and Xenolite nolead Apron (0.35 mm PB), Xenolite nolead Apron (front 0.35 mm PB Mix back 0.25 mm PB, Skirt overlap), Half Apron After shielding with (0.5 mm PB), each scattered dose was measured. During chest computed tomography, the scattered dose of the test site was measured at 272 μSv, and when not shielded with Apron, the average total was 43 μSv, left 81 μSv, right part 82 μSv, posterior part 38.8 μSv, and Gonad part 16 μSv. Became. Xenolite nolead Apron shielded only the upper part and measured all 11.2 μSv, left part 43.1 μSv, right part 45.3 μSv, posterior part 12 μSv and Gonad part 5.2 μSv. Xenolite nolead Apron (Skirt overlap) covered the Pelvis area 360° and the dose was measured to be 5.6 μSv in the whole, 22.4 μSv in the left, 15.7 μSv in the right side, 6 μSv in the posterior part, and 3.2 μSv in the Gonad part. Xenolite nolead Apron (Skirt overlap) covered the Pelvis area 360° and the dose was measured to be 5.6 μSv in the whole, 22.4 μSv in the left, 15.7 μSv in the right side, 6 μSv in the posterior part, and 3.2 μSv in the Gonad part. When measuring only the upper part with Half Apron, the total measurement was 10.7 μSv, the left part 42.6 μSv, the right part 40.6 μSv, the posterior part 11.3 μSv, and the Gonad part 4.7 μSv. The method of 360° shielding of the pelvic area showed a dose reduction of more than 80%, and a dose reduction effect of more than 70% was shown when all shielding was performed. In all computerized tomography examinations, research to reduce the exposure dose and various shielding devices were used. It is believed that continuous research on the technique is needed.

Military Activity and Combat in Hapcheon Area during the Imjin Invasion Period (임진왜란시기 합천지역의 의병 활동과 전투)

  • Kwak, Nak-hyun
    • (The)Study of the Eastern Classic
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    • no.70
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    • pp.257-301
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    • 2018
  • The purpose of the study is to examine righteous army activity and combat in Hapcheon during the Imjin invasion period. The resulting conclusions are as follows. First, Chung In-hong raised a righteous army in Hapcheon and Kim Myeon raised an army in Goryeong. Chung In-hong commanded the army and fought against Japanese army with a leadership of knowing the enemy and himself, and Kim Myeon commanded the army and fought against Japanese military with a leadership of harmony. Second, battles of Chung In-hong's righteous army are the first Mugye battle, the ambush battle in Sawon-dong, the Chogye Majin battle, the Aneon battle, and the third Seongju Castle battle. Battles of Kim Myeon's righteous army are the Yeongang battle, the Gaesanpo battle, the second Mugye battle, the Ucheok-hyeon battle, the Jirye battle, and the Sarangam battle. Battles that Chung In-hong and Kim Myeon united and fought are the first and second Seongju Castle battles. In Jeongyu Jaeran, Chung In-hong played a role of Jodosa who takes charge of provisions in the right area of Gyeongsang. In addition, Hapcheon was the access road and transport route where Japanese army entered Jeolla-do. Third, participation and role of Ming troops are part of restraint device against Japanese army with military tactics of ii chei(using foreigners to control foreigners) to remove Japanese army from Joseon and defend Yodong. After a victory of Li Rusong in Pyeongyang Castle in January, 1593(the 26th year of Seongjo), Ming troops pursued practical interest through peace talks rather than active battles. When there was practically Siege of Jinju, Yujeong troops of Ming entered with hand-to-hand martial arts of Sacheon soldiers in late June, 1593, but did not participate because they should wait for orders of Gyeongnyak Song Eung-chang and Admiral Li Rusong. Fourth, in the Imjin invasion period, Joseon suffered terrible damage such as ruin of the whole country by invasion of Japanese army and in need of aid of military rice from Ming troops, but righteous armies and the royal forces in each area cooperated so defeated Japanese army. It is understood through a case of Hapcheon in the right area of Gyeongsang. Especially, Joseon did not succumb to pressure of Ming troops that used full powers to two aspects such as settlement and battle with Japan during the war, and did not lose national confidence and pride by showing a fighting will to fight against Japanese army to the end with independence. Such a spiritual culture originated from homeland protection and loyalty to the king, and is national spirit of resistance that sublimates the united mind and spirit of community to protect a country against foreign invasion.

Retrieval of Oceanic Skin Sea Surface Temperature using Infrared Sea Surface Temperature Autonomous Radiometer (ISAR) Radiance Measurements (적외선 라디오미터 관측 자료를 활용한 해양 피층 수온 산출)

  • Kim, Hee-Young;Park, Kyung-Ae
    • Journal of the Korean earth science society
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    • v.41 no.6
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    • pp.617-629
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    • 2020
  • Sea surface temperature (SST), which plays an important role in climate change and global environmental change, can be divided into skin sea surface temperature (SSST) observed by satellite infrared sensors and the bulk temperature of sea water (BSST) measured by instruments. As sea surface temperature products distributed by many overseas institutions represent temperatures at different depths, it is essential to understand the relationship between the SSST and the BSST. In this study, we constructed an observation system of infrared radiometer onboard a marine research vessel for the first time in Korea to measure the SSST. The calibration coefficients were prepared by performing the calibration procedure of the radiometer device in the laboratory prior to the shipborne observation. A series of processes were applied to calculate the temperature of the layer of radiance emitted from the sea surface as well as that from the sky. The differences in skin-bulk temperatures were investigated quantitatively and the characteristics of the vertical structure of temperatures in the upper ocean were understood through comparison with Himawari-8 geostationary satellite SSTs. Comparison of the skin-bulk temperature differences illustrated overall differences of about 0.76℃ at Jangmok port in the southern coast and the offshore region of the eastern coast of the Korean Peninsula from 21 April to May 6, 2020. In addition, the root-mean-square error of the skin-bulk temperature differences showed daily variation from 0.6℃ to 0.9℃, with the largest difference of 0.83-0.89℃ at 1-3 KST during the daytime and the smallest difference of 0.59℃ at 15 KST. The bias also revealed clear diurnal variation at a range of 0.47-0.75℃. The difference between the observed skin sea surface temperature and the satellite sea surface temperature showed a mean square error of approximately 0.74℃ and a bias of 0.37℃. The analysis of this study confirmed the difference in the skin-bulk temperatures according to the observation depth. This suggests that further ocean shipborne infrared radiometer observations should be carried out continuously in the offshore regions to understand diurnal variation as well as seasonal variations of the skin-bulk SSTs and their relations to potential causes.

Evaluation Method of Cosmetics for the Effect of Fine Dust Adhesion Prevention Using Floating Chamber (부유챔버를 이용한 화장품의 미세먼지 부착방지 효과 평가법)

  • Kim, Woncheol;Kim, Han Jo;Boo, Yong Chool;Koh, Jae Sook;Baek, Ji Hwoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.46 no.4
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    • pp.319-327
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    • 2020
  • Particulate matters (PM) are small particulate pollution that decrease the function of skin barrier, which causes inflammatory skin diseases and extrinsic aging. In this study, we evaluated the effect of preventing the adherence of PMs from several cosmetic products applied to human skin using iron oxide black. The PM floating chamber consists of skin exposure area, PM inlet, floating power device, and an outlet so that PM can be naturally attached to the skin while floating in the chamber. The change in skin brightness according to the floating concentration of alternative fine dust was checked to confirm the optimal floating concentration conditions. The intensity difference (before-after intensity, Δ) before and after adhesion of iron oxide black was proportional to the amount of PM adhered. The anti-adherence effect of iron oxide black on five cosmetic products were evaluated through 20 each subjects by comparing the amount of iron oxide black adhered on the control and treatment. The difference in brightness before and after the iron oxide black attached to the skin was calculated and compared with the control group(p < 0.05). When over 150 mg of iron oxide black was adhered on the skin, the interference of intensity was low and clearly showed the skin adhered pattern. According to the application of the five cosmetics, the intensity difference was significantly lower than the control group. This means that depending on the product, it prevented the attachment of iron oxide black. This study is a safe and useful method to confirm the prevention of PM skin adherence. In conclusion, cosmetics can prevent the adherence of PM on the skin according to the formulation or ingredients characteristics.

Fabrication of Portable Self-Powered Wireless Data Transmitting and Receiving System for User Environment Monitoring (사용자 환경 모니터링을 위한 소형 자가발전 무선 데이터 송수신 시스템 개발)

  • Jang, Sunmin;Cho, Sumin;Joung, Yoonsu;Kim, Jaehyoung;Kim, Hyeonsu;Jang, Dayeon;Ra, Yoonsang;Lee, Donghan;La, Moonwoo;Choi, Dongwhi
    • Korean Chemical Engineering Research
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    • v.60 no.2
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    • pp.249-254
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    • 2022
  • With the rapid advance of the semiconductor and Information and communication technologies, remote environment monitoring technology, which can detect and analyze surrounding environmental conditions with various types of sensors and wireless communication technologies, is also drawing attention. However, since the conventional remote environmental monitoring systems require external power supplies, it causes time and space limitations on comfortable usage. In this study, we proposed the concept of the self-powered remote environmental monitoring system by supplying the power with the levitation-electromagnetic generator (L-EMG), which is rationally designed to effectively harvest biomechanical energy in consideration of the mechanical characteristics of biomechanical energy. In this regard, the proposed L-EMG is designed to effectively respond to the external vibration with the movable center magnet considering the mechanical characteristics of the biomechanical energy, such as relatively low-frequency and high amplitude of vibration. Hence the L-EMG based on the fragile force equilibrium can generate high-quality electrical energy to supply power. Additionally, the environmental detective sensor and wireless transmission module are composed of the micro control unit (MCU) to minimize the required power for electronic device operation by applying the sleep mode, resulting in the extension of operation time. Finally, in order to maximize user convenience, a mobile phone application was built to enable easy monitoring of the surrounding environment. Thus, the proposed concept not only verifies the possibility of establishing the self-powered remote environmental monitoring system using biomechanical energy but further suggests a design guideline.