• 미생물학회지
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• 제31권1호
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• pp.22-26
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• 1993

Trimorphomyces papilionaceus dikaryon 으로부터 자영적인 돌연변이에 의해 분리한 이배체를 대상으로 생장속도, U. V. 광 조사 후의 생존율, U. V. 분광광도계와 Hoechst 33258 핵염색에 의한 DNA 함량등을 monokaryon 및 dikaryon 과 비교하여 결정하였다. 이배체는 dikaryon 핵의 DNA 양과 비슷하였고 monokaryon 핵의 DNA 양의 2배 정도가 되었다. Glyoxalase II 의 band 양상에서 monokayon은 한 개의 isozyme band 만을 보인 반면, 이배체와 dikaryon은 같은 위치에서 두개의 isozyme band 가 관찰되었다. 이원전개한 전기영동실험에서 dikaryon 특이 단백질은 분자량이 66.000 보다 컸으며 분자량 24,000-29,000 사이에서 이배체 특이 단백질이 존재한다.

• 한국동물학회지
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• 제15권3호
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• pp.101-104
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• 1972

간편하고 경제적이며 재현성도 우수한 cellulose acetate 전기영동장치를 제작하였다. 본 장치를 사용하여 몇가지 동물 조직의 lactate 및 malate dehydrogenase isozyme 을 분리 검출하여 본 결과, 기존 제품으로 실험한 결과와 별로 차이가 없음을 알았다. 흔히 cellulose acetate 전기영동은 일정한 정전류에서 실시하는데, 본 장치에서는 약간의 전류변동이 있는 것이 사실이나 실험결과는 충분히 실용적인 것이었다.

• 한국패류학회지
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• 제12권1호
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• pp.39-45
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• 1996

Polyacrylamide gel electrophoresis was used to reveal the esterase isozyme patterns of a freshwater snail, Semisulcospira gottschei, in the lake Uiam. From the result with inhibitors, it is tentatively concluded that four types (arylesterase, carboxylesterase, acethylesterase, acethylcholinesterase)of the adult(means of allele per locus=1.16, %polymorphism=16.7, heterozygosity=0.082)was lower than the juveniles(A=1.7, %P=16.7, H$\sub$D/=0.2215).

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.552-560
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• 2020

Human carbonic anhydrase (CA) isozyme II has been used as protein target for disorder treatment including glaucoma. Current clinically used sulfonamide-based CA inhibitors can induce side effects, and so alternatives are required. This study aimed to investigate a natural CA inhibitor from Murraya paniculata. The previously developed yeast-based assay was used to screen 14 compounds isolated from M. paniculata and identified by NMR analysis for anti-human CA isozyme II (hCAII) activity. Cytotoxicity of the compounds was also tested using the same yeast-based assay but in a different cultivation condition. Two flavonoid candidate compounds, 5, 6, 7, 8, 3', 4', 5'-heptamethoxyflavone (4) and 3, 5, 7, 8, 3', 4', 5'-heptamethoxyflavone (9), showed potent inhibitory activity against hCAII with a minimal effective concentration of 10.8 and 21.5 μM, respectively, while they both exhibited no cytotoxic effect, even at the highest concentration tested (170 μM). The results from an in vitro esterase assay of the two candidates confirmed their hCAII inhibitory activity with IC₅₀ values of 24.0 and 34.3 μM, respectively. To investigate the potential inhibition mechanism of compound 4, in silico molecular docking was performed using the FlexX and SwissDock software. This revealed that compound 4 coordinated with the Zn²⁺ ion in the hCAII active site through its methoxy oxygen at a distance of 1.60 Å (FlexX) or 2.29 Å (SwissDock). The interaction energy of compound 4 with hCAII was -13.36 kcal/mol. Thus, compound 4 is a potent novel flavonoid-based hCAII inhibitor and may be useful for further anti-CAII design and development.

• Journal of Plant Biology
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• 제17권4호
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• pp.143-156
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• 1974

The purpose of this experiment was to study one side of germination physiology based on that protein profiles and protease relating to protein metabolism, that peroxidase, catalase, $\alpha$-amylase, $\beta$-amylase, and malate dehydrogenase involved in the carbohydrate metabolism of seed germination. All these experiments were divided into the two groups with and without acetone treatment, and were carried out. The protein bands of each germinating stage between the groups treated with and without acetone showed certain basic pattern in polyacrylamide gel disc electrophoresis. However, there was a little difference in the number of protein band, optical density, and migration velocity between two groups. The isozyme bands of peroxidase, and catalase between two groups in polyacrylamide gel disc electrophoresis did not show the numeral difference, but the optical density of certain germinating stage treated with acetone was higher than the group untreated with it and it showed their enzyme activity. The $\alpha$-amylase and $\beta$-amylase activities which involved in starch metabolism of seed germination were higher in the treated group than the other. On one hand, the protease activity of hydrolase occurred in the seeds for germination was also higher, more or less in the treated group than in the other. The isozyme band pattern of malate dehydrogenase in TCA cycle of energy metabolism pathway was very different between two groups growing for 72 hours with and without acetone treatment in cellulose acetate electrophoresis. It indicated that two isozyme bands of malate dehydrogenase was high. Consequently these experimental results mentioned above indicated that acetone treatment before sowing had an effect on dissolving certain complexed lipid substance involved in the seed coats, the activity of carbohydrate hydrolase increased with water absorption which was most comfortable in its germination, dissolved glycerin and fatty acid became certain energy source, and they stimulated the acceleration of respiration metabolism.

• 한국수산과학회지
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• 제29권2호
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• pp.256-261
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• 1996

본 연구는 우리나라의 대표적인 연어과 어류인 은연어, 무지개송어 및 그 잡종3배체의 부화자어에 있어서의 동위효소를 분석하여 부화후 초기의 유전적 특징과 유전현상을 규명하고 이를 토대로 이들 종 및 잡종의 식별을 위한 genetic marker를 찾아보고자 5개의 동위효소에 대한 분석을 실시하였다. 이들 중 PGI에서는 종간 차이를 나타내지 않은 반면에 LDH, MDH, IDH 및 PGM 등은 은연어와 무지개송어간에 종 특이적 pattern을 나타내었는데 이는 이들 어종간의 발생 초기에 종의 식별을 위한 유전적 marker로써 유용한 것으로 판단되며, 또한 잡종 및 잡종3배체의 genetic marker로서도 상기 네 가지의 동위효소는 유용한 것으로 나타났다.

• 한국양식학회지
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• 제9권4호
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• pp.437-444
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• 1996

우리나라 해역에 서식하고 있는 농어 2형(점이 없는 계통과 점이 있는 계통)의 유전학적 분류 및 특징을 조사하기 위하여 isozyme 분석을 실시한 결과, 2형에 있어서 4개의 유전자좌의 분기가 관찰 되었으며 이중 Pt-1 유전자좌는 완전 분기되어 있는 것으로 확인되었다. 총 유전적 변이 보유량은 2형 모두 동일하였으나 점이 없는 계통은 다형 변이 유전좌의 비가 높았으며, 점이 있는 계통은 변이 유전자좌의 비가 높은 것으로 나타났다. 2형에 있어서 평균 이형 접합체율의 기대치에 차이가 나타나 점이 없는 계통과 점이 있는 계통은 유전적으로 집단구조가 다름이 확인 되었다. 2형간의 유전적 분화 정도를 나타내는 유전적 거리(D)의 값은 0.12036이었으며, 분화연도는 $6.2{\times}10^5$년전 인 것으로 추정되었다.

• 약학회지
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• 제38권4호
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• pp.430-439
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• 1994

S-Adenosyl-L-methionine synthetase (ATP: methionine S-Adenosyltransferase, EC 2.5.1.6; AdoMet synthetase) catalyzes the biosynthesis of S-Adenosyl-L-methionine(AdoMet) from methionine in the presence of ATP. To elucidate the role of transmethylation reaction in the pancreatic tissues, we examined AdoMet synthetase and isozyme activities, and AdoMet contents in the various tissues. The activities of AdoMet synthetase marked the highest in the kidney, and the lowest in the testis among the various tissues of rat. Considerable amounts of AdoMet synthetase activities were detected in the pancreatic tissues of various animals except for those of frog. The level of ${\alpha}$ and ${\gamma}$ isozyme activities were present in the pancreatic tissues of various animals, while ${\beta}$ isozyme activities were detected as trace. AdoMet synthetase activities of rat brain, liver, testis were decreased with growth. In the rat pancreatic tissues, AdoMet synthetase activities were increased during 16 days after birth and then decreased between 16 and 47 days of age. Levels of AdoMet contents of rat brain and testis were decreased with growth. However, AdoMet contents of rat pancreas were decreased until 26 days of age, and then increased thereafter. AdoMet synthetase isozyme patterns did not vary with growth in the pancreas and testis. But, in the liver, ${\beta}$ form is strikingly increased with growth.

• 한국작물학회지
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• 제47권3호
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• pp.206-210
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• 2002

종실 등의 특성을 기준으로 분류한 7가지 벼 이형주군의 변이와 분리양상은 다음과 같다. 1. Esterase 동위효소 분석에서 장립적미군은 1과 3 esterase isozyme zymogram(EIZ), 장립메성군은 1, 3 및 7 EIZ로 분류할 수 있었다. 2. 극만생 불임군은 EIZ가 1, 2, 1+2, 5, 6, 5+6, 7, 8, 7+8 및 12로 다양하게 분리되었다. 3. EIZ가 1인 장립적미군이 3 EIZ인 장립적미군에 비해 간장이 길고 현미의 장폭비가 낮았다. 4. EIZ가 3인 장립메성군은 1이나 7 EIZ인 장립메성군에 비해 간장이 길고 수장이 짧고 분얼수가 많고 현미의 장폭비가 낮으며 수당 립수가 적었다.

• 한국작물학회지
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• 제47권1호
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• pp.29-35
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• 2002

This study was carried out to investigate the characteristics of shattering habit and germination of offtype rice plants collected in Korea which were classified into seven groups based on grain characteristics. In the long-grain red rice group, the short-grain red rice group, the long-grain normal rice group, and the long-grain waxy rice group, the tensile strength of grain and primary rachis branch was relatively low and the fiber cell of the primary rachis branch was short. Characteristics of shattering-related traits and germination in relation to grain and esterase isozyme zymogram type of off-type rice plants. In most lines of the extremely late and sterile rice group, the tensile strength of grain was low, while that of primary rachis branch was high, and the fiber cell was long. However, in the type 1 esterase isozyme zymogram(EIZ) lines among the long-grain normal rice group and the type 6 and 5+6 EIZ plants among the extremely late and sterile rice group, the tensile strength of grain was relatively higher. The long-grain waxy rice group and the type 3 EIZ lines among the long-grain red rice group, showed higher germinability than did cultivars which are known to germinate well even in low-temperature. The other off-type rice group, except for the short-grain waxy rice group and type 1 and 7 EIZ lines among the long-grain normal rice group, had higher germination speed than that of the cultivars.