• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.838-848
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• 2012

Orally administered herbal glycosides are metabolized to their hydrophobic compounds by intestinal microflora in the intestine of animals and human, not liver enzymes, and absorbed from the intestine to the blood. Of these metabolites, some, such as quercetin and kaempherol, are mutagenic. The fecal bacterial enzyme fraction (fecalase) of human or animals has been used for measuring the mutagenicity of dietary glycosides. However, the fecalase activity between individuals is significantly different and its preparation is laborious and odious. Therefore, we developed a fecal microbial enzyme mix (FM) usable in the Ames test to remediate the fluctuated reaction system activating natural glycosides to mutagens. We selected, cultured, and mixed 4 bacteria highly producing glycosidase activities based on a cell-free extract of feces (fecalase) from 100 healthy Korean volunteers. When the mutagenicities of rutin and methanol extract of the flos of Sophora japonica L. (SFME), of which the major constituent is rutin, towards Salmonella typhimurium strains TA 98, 100, 102, 1,535, and 1,537 were tested using FM and/or S9 mix, these agents were potently mutagenic. These mutagenicities using FM were not significantly different compared with those using Korean fecalase. SFME and rutin were potently mutagenic in the test when these were treated with fecalase or FM in the presence of S9 mix, followed by those treated with S9 mix alone and those with fecalase or FM. Freeze-dried FM was more stable in storage than fecalase. Based on these findings, FM could be usable instead of human fecalase in the Ames test.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.12B
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• pp.1112-1122
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• 2008

This paper reviews the Parlay X and proposes SIP multimedia session control mechanism which is able to be implemented over IMS using Extended Call Control APIs of Parlay X. Parlay X is one of the industrial standards of Open API in the telecommunication to open network resources and capabilities to third party service providers. In this paper, SIP session control mechanism is described by depicting call flows of basic and essential session handling methods including session initiation, transfer, restoration, and termination. We also show how easy Parlay X Extended Call Control APIs can be used for the purpose of complicated calls handling in the IT applications. To verify feasibility of the blended services based on convergence of telecommunication and internet with regard to performance, we accomplish experimental performance of Extended Call Control APIs from IT application through open service gateway. We show Open API could be applied to next generation network based on IMS without serious degradation of the network performance.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.5
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• pp.41-54
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• 2018

In recent years, as interest in youth startup has increased, the Korean government has established various policies to support young entrepreneurs and actively implements such policies. Especially, as a way to promote youth startup, the development of startup clubs in universities is being suggested, and the importance of startup clubs is growing day by day. As reflected in these points, the Korean government is increasing its budget every year to support the startup clubs of universities, and actively supports the funds and space required for the startup clubs of universities. Unfortunately, however, despite the growing importance of startup clubs and the active support of the government for the startup clubs, academic attempts to see how effectively the startup clubs are operating are not enough. For this reason, this study analyzes the effect of entrepreneurship of university start-up club members on the effectiveness of start-up club. More specifically, in this study, entrepreneurship of club members is classified as risk-taking propensity, proactiveness, and innovativeness, as emphasized in previous researches. We empirically analyzed the effects of these independent variables on the effectiveness of the start-up clubs, focusing on the moderating effect of club members' diversity. We conducted empirical analyses on 1112 start-up club members from 12 universities located in Jeollanam-do and Jeollabuk-do, and the analysis results are as follows. First, the entrepreneurship of the start-up club members, namely risk taking propensity, proactiveness and innovativeness, all have a positive effect on the effectiveness of the start-up club. These results are consistent with the emphasis of many previous studies. The higher the tendency to take risks, the more inclined to move forward through difficulties, and the higher the tendency to break out of the existing framework and pursue new things, suggesting that the effectiveness of the club is enhanced. Second, as a result of verifying the moderating effect of the club members' diversity, it was found that club members' diversity moderates the relationship between the proactiveness of club members and club effectiveness in the negative (-) direction. In other words, the positive effect of the entrepreneurship of club members on the effectiveness of the start-up club means that the proactiveness is weakened in the case of a club having members from different major backgrounds. The results of this study are expected to provide meaningful theoretical and practical implications by identifying the influencing factors of the start-up club's effectiveness, which has not received much attention so far.

• Journal of Korean Academy of Nursing
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• v.35 no.6
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• pp.1101-1112
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• 2005

Purpose: This study was done to suggest directions for developing exercise interventions for fall prevention in the elderly in Korea in the future, Method: Twenty five articles for fall prevention exercises were reviewed and analyzed. Result: $84.0\%$ of subjects were older adults age 65 and older living in the community. The most frequently performed interventions were lower limb strength and balance exercises together $43.3\%$, group exercise $70.0\%$, exercise 3 times/week $60.0\%$, 60 min per session $36.7\%$, duration of 12 weeks and 1 year $23.3\%$ each. The most frequently used outcome variables were static balance $84.0\%$, lower limb muscle strength $72.0\%$, dynamic balance $56.0\%$, and falls $56.0\%$. The effect of exercise interventions on fall prevention was inconclusive. Lower limb strength exercises with resistance were effective for increasing muscle strength. Balance exercises with various movements for balance were effective for increasing balance. Conclusion: Exercise interventions for fall prevention is recommended for older adults with risk factors of falling. The desirable type of exercise intervention is lower limb strength and balance exercise together.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.12
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• pp.1105-1112
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• 2013

In this study, a method for predicting heating loads using building characteristic coefficients is proposed for heating system control, and a method for predicting hourly temperature and solar insolation, which mainly affect building heating loads, is also proposed. The temperature and solar insolation are predicted by using a fuzzy theory from forecast information at the meteorological agency, and the building characteristic coefficients for the prediction of heating loads are derived from EnergyPlus. The simulated heating loads of the present study show good agreement with those of EnergyPlus. and the variations of the predicted heating loads using the predicted temperature and solar insolation are similar to those using the actual weather data.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1133-1140
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• 2012

Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the down-regulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1112-1119
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• 2017

The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by a decompression-mediated (vacuum) enrichment method (VE). As the results, ethanol was more efficiently produced from HR when sugar solutions were concentrated by VE following EH (EH/VE). Using multiple feeding of the sugar solution prepared by EH/VE from HR, ethanol reached up to a concentration of 54.3 g/l, corresponding to 24.9 g/100 g dried material, which attained the economic level of product concentration (approximately 5%). The results indicate that by using HR, it is feasible to establish a bioethanol production process, which is effective for using microalgae as the raw material for ethanol production.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.8
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• pp.1109-1112
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• 2000

A field experiment was carried out to determine the effects of mechanical and chemical conditioning at mowing on field drying rate and quality of grass hay in 1996. Mower conditioner and/or chemical drying agent $(K_2CO_3)$ were used at different harvest stages (late boot, heading and bloom stages) for hastening hay-making. After field drying, square bales were made by hay baler, and the visual estimation and nutritive value of hay were evaluated after storing two months. In mower conditioning, the duration of field drying was shortened by 0.5 to 1 day compared with drying agent, and by 1 to 2 days compared with control. The drying matter loss of hay was reduced by late harvest and mechanical conditioning. The visual score (leafiness, green color, odor and softness), and acid detergent fiber (ADF), neutral detergent fiber (NDF), in vitro dry matter digestibllity (IVDMD), and relative feed value (RFV) of hay were improved with mechanical conditioning, but chemical alone had little effect on quality. The quality of hay harvested at bloom stage was much lower than that of hay harvested at late boot and heading stage. In conclusion, mower conditioning can enhance the field drying rate of grass hay, however the drying efficiency of chemical drying agent is very low. Also the effects of chemical/mechanical combined conditioning are very similar compared with mechanical conditioning alone. Harvesting at late boot to heading stage is recommended for the production of high quality hay.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.1109-1112
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• 2015

A milatary ship without degaussing coil has a vertical magnetization to compensate magnetization induced by the vertical magnetic field component of earth magnetic field during demagnetization process. Flash D is very useful to acquire vertical magnetization. However this is hard to predict vertical magnetization. This experiment was investicated on another method, which used the only vertical bias magentic field. The specimens were prepared by thin Zn coated steel sheet with a thickness of 0.15mm. The shapes of 3 specimes was rectangular, triangular and circular cylinders. These shapes were corresponded to the shapes of bow, mid and stern of a vessel. Through FEM analysis, the difference of magnetic signatures for these specimens was recognized and the residual magnetization curve was measured. magnetic field was generated by a solenoid coil and magnetic signature was measured by a magnetic field sensor. A linearity between a vertical bias magnetic field and a vertical manetzation existed and the vertical magnetization of a miltary vessel was predicted by the linearity.

• Journal of Microbiology and Biotechnology
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• v.22 no.5
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• pp.659-667
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• 2012

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.