• Journal of Microbiology
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• v.43 no.2
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• pp.183-190
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• 2005

Staphylococcus aureus is known to be capable of utilizing transferrin-bound iron, via both siderophore­and transferrin-binding protein (named IsdA)-mediated iron-acquisition systems. This study was designed in order to determine which iron-acquisition system plays the essential or dominant role with respect to the acquisition of iron from human transfenin, in the growth of S. aureus. Holotransferrin (HT) and partially iron-saturated transferrin (PT), but not apotransferrin (AT), were found to stimulate the growth of S. aureus. S. aureus consumed most of the transferrin-bound iron during the exponential growth phase. Extracellular proteases were not, however, involved in the liberation of iron from transferrin. Transferrin-binding to the washed whole cells via IsdA was not observed during the culture. The expression of IsdA was observed only in the deferrated media with AT, but not in the media supplemented with PT or HT. In contrast, siderophores were definitely produced in the deferrated media with PT and HT, as well as in the media supplemented with AT. The siderophores proved to have the ability to remove iron directly from transferrin, but the washed whole cells expressing IsdA did not. In the bioassay, the growth of S. aureus on transferrin-bound iron was stimulated by the siderophores alone. These results demonstrate that the siderophore-mediated iron-acquisition system plays a dominant and essential role in the uptake of iron from transferrin, whereas the IsdA-mediated iron-acquisition system may play only an ancillary role in the uptake of iron from transferrin.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.4
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• pp.66-73
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• 2000

Alkaline sulfite-anthraquinone(AS-AQ) cooking process has been applied to unground pine bark. The properties of the spent liquor such as molecular weight distributions, sulfonic equivalent weights, degree of sulfonation, phenolic hydroxyl groups and kaolin dispersing ability have been investigated to evaluate the possibility of using this liquor as concrete additives or binders. In the case of ground bark meal, more than 90% delignification was achieved at the optimal cooking conditions. However, applying these conditions on unground bark the delignification was slightly retarded. The decrease in the degree of delignification may be attributed to less penetration and diffusion of chemicals during the cooking of the bark. Increasing the cooking temperature only by $5^{\circ}C$ improved the delignification of the bark and about 90% delignification can be achieved. These results indicate that bark can be used successfully during AS-AQ cooking without any mechanical or physical pretreatment. The properties of lignin or polyphenol sulfonates in the spent liquor after AS-AQ cooking of the bark were compared with Sunflo-R, which is commercial lignosulfonate(CLS) prepared from wood. The weight average molecular weights(Mws) estimated by gel-filteration chromatography was found to be ranging from 1,200~1,800. These are considerably lower than those in CLS. Lignin or polyphenol sulfonates in the spent liquor of bark and CLS have similar degree of sulfonation, but the phenolic hydroxyl group was 1.8 times higher than CLS. Moreover, the dispersing abilities of the spent liquors were better than that of CLS, especially after 0.1% addition to kaolin suspension.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.57-67
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• 2014

Purpose: The modern IT can give enormous impacts on the agricultural production and consumption. However, farmer's ability to use IT devices has been known as one of the critical factors on success of IT in agriculture. This survey study was performed to evaluate the informatization status of farmers and to draw a strategy to develop and distribute a ubiquitous agricultural information system. Methods: A survey questionnaire with 19 questions on the degree of IT devices such as computers and smartphones and their utilization were developed. The survey was questioned 3 groups of farmers in Gyounggi Province and analyzed statistically by the ${\chi}^2$ test. Results: The order of IT devices distribution were computers, smartphones, smart TVs and tablet PCs in all groups. The ratios of ownerships of the devices are 97.7, 78.6%, 31.4%, 17.8% respectively. The active farmers in this survey showed higher informatization level than that of native and the general farmers. Conclusions: The ubiquitous agricultural information system was judged to be developed for the computer because spread and use of it exceeds the other devices. Also, the information system based on the smartphone could be a complementary way if the rapid of the smartphones continues and proper education on use of the phone is provided.

• Journal of Industrial Convergence
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• v.18 no.1
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• pp.65-71
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• 2020

Recently, the field of using data has begun to attract attention in professional sports. In the field of data utilization, in addition to the classic records obtained within the economy, secondary records that emphasize efficiency are also actively used. Therefore, in this study, we try to study the correlation with the pitcher's strikeout ability through the daily average humidity, which is data outside the competition. For this reason, referring to the daily average record of the area of the home base of 10 teams belonging to the KBO league and the auxiliary stadium, the top 5 in the win, hold, save section to grasp the characteristics of the starting pitcher and the rescue pitcher We analyzed K / 9 records for each person. Through the results of this study, we found a significant difference in the K / 9 record between the starting pitcher and the rescue pitcher, and we can expect to investigate the use of professional sports data and develop the industry in general.

• Journal of Korean Academy of Nursing
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• v.46 no.6
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• pp.881-893
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• 2016

Purpose: The purpose of this study was to develop a heart health diary to promote self-care ability among patients with heart failure (HF), and to identify the diary's effect on self-care adherence, self-efficacy, and physical activity. Methods: A randomized control-group pretest-posttest design was adopted using block randomization. A calender-typed health diary was developed and it included a self-care checklist and education information on HF management. The experimental group were given guided counseling and education for 8 weeks and wrote a daily health diary during that period. Data were collected from the outpatient department of a tertiary medical center from February to April 2016. To verify the hypotheses, data for the experimental group (n=28) and control group (n=33) were analysed using the independent t-test with SPSS/WIN 21.0. Results: At the end of 8 weeks the experimental group had significantly higher scores for self-care adherence (t=-2.48, p =.016) and exercise related self-efficacy (t=-3.44, p =.001) compared to the control group. Conclusion: The findings show that the application of a patient-directed heart health diary is an effective nursing intervention for improving HF patients' self-care adherence and exercise self-efficacy. Strategies to promote dietary self-efficacy are necessary along with further studies including repeated research with an increasing intervention period. Healthcare providers need to encourage the utilization of a health diary for HF patients as a tool for evaluation and for implementation that leads to self-care.

• The KIPS Transactions:PartD
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• v.9D no.1
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• pp.75-80
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• 2002

Putnam develops SLIM (Software LIfecycle Management) model based upon the assumption that the manpower utilization during software project development is followed by a Rayleigh distribution. To obtain the manpower distribution, we have to be estimate the total development effort and difficulty ratio parameter. We need a way to accurately estimate these parameters early in the requirements and specification phase before investment decisions have to be made. Statistical tests show that system attributes are highly correlation (redundant) so that Putnam discards one and get a parameter estimator from the other attributes. But, different statistical method has different system attributes and presents different performance. To select the principle system attributes, this paper uses the principle component analysis (PCA) instead of Putnam's method. The PCA's results improve a 9.85 percent performance more than the Putnam's result. Also, this model seems to be simple and easily realize.

• Journal of Korean Society of Archives and Records Management
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• v.20 no.2
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• pp.41-65
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• 2020

Organizations always face the risk of litigation; thus, they should actively prepare for it. In particular, public enterprises must respond to lawsuits carefully to avoid any management risk and possible damage of publicity. Given this, this study aims to identify the relationship between efficient litigation response and record management, determine problems related to record management while utilizing records as evidence, and propose measures to improve record management that uses records as legal evidence. For records to be deemed as legal evidence, RMS is essential to ensure authenticity. Moreover, the ability to secure potential evidence and awareness of the importance of records management needs to be validated. The Commission also stressed the importance of cooperation between archivists and the legal team to efficiently respond to lawsuits.

• Journal of Microbiology and Biotechnology
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• v.21 no.11
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• pp.1151-1158
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• 2011

In this study, a galactooligosaccharide (GOS) was synthesized using active ${\beta}$-galactosidase (${\beta}$-gal) inclusion bodies (IBs)-containing Escherichia coli (E. coli) cells. Analysis by MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrometry revealed that a trisaccharide was the major constituent of the synthesized GOS mixture. Additionally, the optimal pH, lactose concentration, amounts of E. coli ${\beta}$-gal IBs, and temperature for GOS synthesis were 7.5, 500 g/l, 3.2 U/ml, and $37^{\circ}C$, respectively. The total GOS yield from 500 g/l of lactose under these optimal conditions was about 32%, which corresponded to 160.4 g/l of GOS. Western blot analyses revealed that ${\beta}$-gal IBs were gradually destroyed during the reaction. In addition, when both the reaction mixture and E. coli ${\beta}$-gal hydrolysate were analyzed by high-performance thin-layer chromatography (HP-TLC), the trisaccharide was determined to be galactosyl lactose, indicating that a galactose moiety was most likely transferred to a lactose molecule during GOS synthesis. This GOS synthesis system might be useful for the synthesis of galactosylated drugs, which have recently received significant attention owing to the ability of the galactose molecules to improve the drugs solubility while decreasing their toxicity. ${\beta}$-Gal IB utilization is potentially a more convenient and economic approach to enzymatic GOS synthesis, since no enzyme purification steps after the transgalactosylation reaction would be required.

• Nano
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• v.13 no.10
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• pp.1850118.1-1850118.17
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• 2018

Nanoparticles of the semiconductor catalyst $Cd_xZn_{1-x}S$ were embedded into the metal organic framework MIL-101(Cr) to obtain $Cd_xZn_{1-x}S@MIL-101$(Cr) nanocomposites. These materials not only possess high surface areas and mesopores but also show good utilization of light energy. The ultraviolet-visible diffuse reflectance patterns of $Cd_xZn_{1-x}S@MIL-101$(Cr) nanocomposites showed that $Cd_{0.8}Zn_{0.2}S@MIL-101$(Cr) possessed good visible light response ability among the synthesized nanocomposites. The photocatalytic performance of the $Cd_xZn_{1-x}S@MIL-101$(Cr) nanocomposites were tested via degradation and mineralization of methylene blue in neutral water solution under light irradiation using a 300W xenon lamp. As a result, using $Cd_{0.8}Zn_{0.2}S@MIL-101$(Cr) as a catalyst, 99.2% of methylene blue was mineralized within 30 min. Due to the synergistic effect of adsorption by the MIL-101(Cr) component and photocatalytic degradation provided by the $Cd_{0.8}Zn_{0.2}S$ component, the $Cd_{0.8}Zn_{0.2}S@MIL-101$(Cr) catalyst displayed superior photocatalytic performance relative to $Cd_{0.8}Zn_{0.2}S$ and MIL-101(Cr). Furthermore, $Cd_{0.8}Zn_{0.2}S@MIL-101$(Cr) possessed excellent stability during photodegradation and exhibited good reusability. The remarkable photocatalytic performance of $Cd_{0.8}Zn_{0.2}S@MIL-101$(Cr) is likely due to the effective transfer of electrons and holes at the heterojunction interfaces.

• Applied Chemistry for Engineering
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• v.22 no.3
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• pp.332-335
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• 2011

As our interests in eco-friendly materials have been significantly increased, the utilization of porous zeolite concrete that has structural functionality and permeability has been increased. In this paper, the mixture of porous concrete and zeolite, which can be used as multirole boulders, was investigated for the suitability of an environment-friendly product by evaluating of the water purification ability. The contamination removal rates of BOD, TOC, T-N, and T-P in stagnant water tank were 70.6, 67.0, 57.7, and 50.6%, respectively. Also for the non-point source pollution with the inflow and the outflow, the removal rates of Zn, Pb, BOD, and COD were 99.9, 90.0, 69.2, and 33.5%, respectively. The performance of the heavy metal contamination removal for the porous zeolite showed better than that of stagnant system. Therefore, it is expected that the installation of the porous zeolite concrete can play a role as an eco-friendly products by its high contamination removal.