• 한국전자파학회논문지
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• 제23권9호
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• pp.1087-1093
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• 2012

본 논문에서는 ASK(Amplitude Shift Keying) 송신기, 한 쌍의 루프 안테나 및 ASK 수신기로 구성되는 HF-대역 자기장 통신 시스템을 구현하였다. 특히, E급 증폭기를 사용하는 ASK 송신기의 데이터 변조 방법으로 Drain 바이어스 전압을 입력 데이터에 따라 두 가지 레벨로 가변하여 공급하는 바이어스 스위칭 회로를 새롭게 제안하였다. E급 증폭기는 저가의 IRF510 power MOSFET를 이용하여 6.78 MHz에서 최대 5 W 출력과 동작 바이어스 전체에서 75 % 이상의 효율이 측정되었다. ASK 수신기는 Log 증폭기, 필터 및 비교기로 구현하여 -78 dBm의 수신 감도를 구현하였다. 자기장 통신 시스템의 최대 통신 거리를 예측하기 위하여 근역장과 원역장에서의 자기 장 유도식을 활용하여 전송 손실을 계산하는 방법을 고안하였다. 또한, $30{\times}30cm^2$ 크기의 사각형 루프 안테나쌍 을 이용한 실내 전송 실험을 수행하여 제시한 방법의 타당성을 확인하였다. 전송 손실 추정 결과, 1 W 출력과 -70 dBm 수신 감도를 가질 경우 최대 35 m의 수신거리가 계산되었다. 최종적으로 설계된 ASK 송신부와 ASK 수신부를 루프 안테나 쌍에 연결하여 5 m 거리에서 통신이 이루어짐을 확인하였다.

• 한국전자파학회논문지
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• 제24권9호
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• pp.883-891
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• 2013

스마트폰 무선 충전 시나리오에서는 송신 패드에 비해 수신 패드의 크기가 작으므로 수신 패드의 위치에 상관없이 일정한 전력을 부하에 공급하는 것이 중요하다. 본 논문에서는 송신 패드와 수신 패드의 크기가 각각 $16cm{\times}18cm$와 $6cm{\times}8cm$의 직사각형 구조를 갖는 경우, 무선 전력 전송 송신부에 위치한 E급 증폭기의 Drain 바이어스 전압만을 조정하여 수신 패드의 위치에 상관없이 일정한 전력이 부하에 공급되는 방식을 제안하였다. 설계된 LF-대역 무선 전력 시스템의 구성은 PWM IC인 TL494로 제어되는 Buck converter 구조의 전원 회로, 저가의 IRF510 power MOSFET을 이용한 E급 증폭기, 송신 패드 및 수신 패드, 그리고 Schottky 다이오드를 이용한 풀 브릿지 정류기로 구성된다. 제작된 무선 전력 전송 시스템은 바이어스 조정을 하지 않는 경우 240 kHz에서 최대 4 W 출력과 67 % 이상의 시스템 효율을 가지며, 바이어스 조정을 하는 경우에는 수신 패드의 위치에 상관없이 수신 전력을 2 W로 일정하게 유지할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1665-1674
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• 2019

Zika virus (ZIKV) is a mosquito-transmitted, emerging Flavivirus that causes Guillain-$Barr{\acute{e}}$ syndrome and microcephaly in adults and fetuses, respectively. Since ZIKV was first isolated in 1947, severe outbreaks have occurred at various places worldwide, including Yap Island in 2007, French Polynesia in 2013, and Brazil in 2015. Although incidences of ZIKV infection and dissemination have drastically increased, the mechanisms underlying the pathogenesis of ZIKV have not been sufficiently studied. In addition, despite extensive research, the exact roles of individual ZIKV genes in the viral evasion of the host innate immune responses remain elusive. Besides, it is still possible that more than one ZIKV-encoded protein may negatively affect type I interferon (IFN) induction. Hence, in this study, we aimed to determine the modulations of the IFN promoter activity, induced by the MDA5/RIG-I signaling pathway, by over-expressing individual ZIKV genes. Our results show that two nonstructural proteins, NS2A and NS4A, significantly down-regulated the promoter activity of IFN-${\beta}$ by inhibiting multiple signaling molecules involved in the activation of IFN-${\beta}$. Interestingly, while NS2A suppressed both full-length and constitutively active RIG-I, NS4A had inhibitory activity only on full-length RIG-I. In addition, while NS2A inhibited all forms of IRF3 (full-length, regulatory domain-deficient, and constitutively active), NS4A could not inhibit constitutively active IRF3-5D. Taken together, our results showed that NS2A and NS4A play major roles as antagonists of MDA5/RIG-I-mediated IFN-${\beta}$ induction and more importantly, these two viral proteins seem to inhibit induction of the type I IFN responses in differential mechanisms. We believe this study expands our understanding regarding the mechanisms via which ZIKV controls the innate immune responses in cells and may pave the way to development of ZIKV-specific therapeutics.

• Journal of Ginseng Research
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• 제39권1호
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• pp.61-68
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• 2015

Background: Korean Red Ginseng (KRG) is a representative traditional herbal medicine with many different pharmacological properties including anticancer, anti-atherosclerosis, anti-diabetes, and anti-inflammatory activities. Only a few studies have explored the molecular mechanism of KRG-mediated anti-inflammatory activity. Methods: We investigated the anti-inflammatory mechanisms of the protopanaxadiol saponin fraction (PPD-SF) of KRG using in vitro and in vivo inflammatory models. Results: PPD-SF dose-dependently diminished the release of inflammatory mediators [nitric oxide (NO), tumor necrosis factor-${\alpha}$, and prostaglandin $E_2$], and downregulated the mRNA expression of their corresponding genes (inducible NO synthase, tumor necrosis factor-${\alpha}$, and cyclooxygenase-2), without altering cell viability. The PPD-SF-mediated suppression of these events appeared to be regulated by a blockade of p38, c-Jun N-terminal kinase (JNK), and TANK (TRAF family member-associated NF-kappa-B activator)-binding kinase 1 (TBK1), which are linked to the activation of activating transcription factor 2 (ATF2) and interferon regulatory transcription factor 3 (IRF3). Moreover, this fraction also ameliorated HCl/ethanol/-induced gastritis via suppression of phospho-JNK2 levels. Conclusion: These results strongly suggest that the anti-inflammatory action of PPD-SF could be mediated by a reduction in the activation of p38-, JNK2-, and TANK-binding-kinase-1-linked pathways and their corresponding transcription factors (ATF2 and IRF3).

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.942-948
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• 2021

Canine influenza virus (CIV) induces acute respiratory disease in dogs. In this study, we aimed to determine the signaling pathways leading to the induction of IFN-β in a canine respiratory epithelial cell line (KU-CBE) infected with the H3N2 subtype of CIV. Small interfering RNAs (siRNAs) specific to pattern recognition receptors (PRRs) and transcription factors were used to block the IFN-β induction signals in H3N2 CIV-infected KU-CBE cells. Among the PRRs, only the TLR3 and RIG-I expression levels significantly (p < 0.001) increased in CIV-infected cells. Following transfection with siRNA specific to TLR3 (siTLR3) or RIG-I (siRIG-I), the mRNA expression levels of IFN-β significantly (p < 0.001) decreased, and the protein expression of IFN-β also decreased in infected cells. In addition, co-transfection with both siTLR3 and siRIG-I significantly reduced IRF3 (p < 0.001) and IFN-β (p < 0.001) mRNA levels. Moreover, the protein concentration of IFN-β was significantly (p < 0.01) lower in cells co-transfected with both siTLR3 and siRIG-I than in cells transfected with either siTLR3 or siRIG-I alone. Also, the antiviral protein MX1 was only expressed in KU-CBE cells infected with CIV or treated with IFN-β or IFN-α. Thus, we speculate that IFN-β further induces MX1 expression, which might suppress CIV replication. Taken together, these data indicate that TLR3 and RIG-I synergistically induce IFN-β expression via the activation of IRF3, and the produced IFN-β further induces the production of MX1, which would suppress CIV replication in CIV-infected cells.

• 해양환경안전학회지
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• 제28권7호
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• pp.1222-1230
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• 2022

로터 블레이드는 조류발전 터빈의 매우 중요한 구성 요소로서, 해수의 높은 밀도로 인해 큰 추력(Trust force)와 하중(Load)의 영향을 받는다. 따라서 블레이드의 형상 및 구조 설계를 통한 성능과 복합소재를 적용한 블레이드의 구조적 안전성을 반드시 확보해야 한다. 본 연구에서는 블레이드 설계 기법인 BEM(Blade Element Momentum) 이론을 이용해 1MW급 대형 터빈 블레이드를 설계하였으며, 터빈 블레이드의 재료는 강화섬유 중의 하나인 GFRP(Glass Fiber Reinforced Plastics)를 기본으로 CFRP(Carbon Fiber Reinforced Plastics)를 샌드위치 구조에 적용해 블레이드 단면을 적층(Lay-up)하였다. 또한 유동의 변화에 따른 구조적 안전성을 평가하기 위해 유체-구조 연성해석(Fluid-Structure Interactive Analysis, FSI) 기법을 이용한 선형적 탄성범위 안의 정적 하중해석을 수행하였으며, 블레이드의 팁 변형량, 변형률, 파손지수를 분석해 구조적 안전성을 평가하였다. 결과적으로, CFRP가 적용된 Model-B의 경우 팁 변형량과 블레이드의 중량을 감소시켰으며, 파손지수 IRF(Inverse Reserce Factor)가 Model-A의 3.0^*V_r를 제외한 모든 하중 영역에서 1.0 이하를 지시해 안전성을 확보할 수 있었다. 향후 블레이드의 재료변경과 적층 패턴의 재설계뿐 아니라 다양한 파손이론을 적용해 구조건전성을 평가할 예정이다.

• Clinical and Experimental Reproductive Medicine
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• 제50권1호
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• pp.44-52
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• 2023

Objective: The DNA integrity of spermatozoa that attach to fallopian tube (FT) cells is higher than spermatozoa that do not attach. FT epithelial cells can distinguish normal and abnormal sperm chromatin. This study investigated the effects of sperm with a high-DNA fragmentation index (DFI) from men with unexplained repeated implantation failure (RIF) on the Toll-like receptor (TLR) signaling pathway in human FT cells in vitro. Methods: Ten men with a RIF history and high-DFI and 10 healthy donors with low-DFI comprised the high-DFI (>30%) and control (<30%) groups, respectively. After fresh semen preparation, sperm were co-cultured with a human FT epithelial cell line (OE-E6/E7) for 24 hours. RNA was extracted from the cell line and the human innate and adaptive immune responses were tested using an RT2 profiler polymerase chain reaction (PCR) array. Results: The PCR array data showed significantly higher TLR-1, TLR-2, TLR-3, TLR-6, interleukin 1α (IL-1α), IL-1β, IL-6, IL-12, interferon α (IFN-α), IFN-β, tumor necrosis factor α (TNF-α), CXCL8, GM-CSF, G-CSF, CD14, ELK1, IRAK1, IRAK2, IRAK4, IRF1, IRF3, LY96, MAP2K3, MAP2K4, MAP3K7, MAP4K4, MAPK8, MAPK8IP3, MYD88, NFKB1, NFKB2, REL, TIRAP, and TRAF6 expression in the high-DFI group than in the control group. These factors are all involved in the TLR-MyD88 signaling pathway. Conclusion: The MyD88-dependent pathway through TLR-1, TLR-2, and TLR-6 activation may be one of the main inflammatory pathways activated by high-DFI sperm from men with RIF. Following activation of this pathway, epithelial cells produce inflammatory cytokines, resulting in neutrophil infiltration, activation, phagocytosis, neutrophil extracellular trap formation, and apoptosis.

• Journal of electromagnetic engineering and science
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• 제3권2호
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• pp.86-90
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• 2003

A flat conversion loss in microwave mixer is hard to achieve if integrating with an image rejection filter(IRF). This is due to the change of termination condition with respect to the LO and IF frequency at RF port where the filter has 50 ohm termination property only in the RF band. This paper describes a flatness maintenance in the down mixer concerning a diode matching condition as well as an electrical length of embedding line at RF port. The implemented single balance diode mixer is suitable for a 23 ㎓ European Terrestrial Radio. RF, LO and fixed IF frequency chosen in this paper are 21.2∼22.4 ㎓, 22.4∼23.6 ㎓ and 1.2 ㎓, respectively. The measured results show a conversion loss of 8.5 ㏈, flatness of 1.2 ㏈ p-p, input P1㏈ of 7㏈m, IIP3 of 15.42 ㏈m with nominal LO power level of 10㏈m. The return loss of RF and LO port are less than - 15 ㏈ and - 12 ㏈, respectively and IF port is less than - 6 ㏈. LO/RF and LO/IF isolation are 18 ㏈ and 50 ㏈, respectively. This approach would be a helpful reference for designing up/down converter possessing a filtering element.

• Molecules and Cells
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• 제38권9호
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• pp.759-764
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• 2015

Tripartite motif protein 25 (TRIM25), mediates K63-linked polyubiquitination of Retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. Here, we demonstrate that TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and MAVS mediated activation of NF-${\kappa}B$ and interferon production. TRIM25 is required for the full activation of NF-${\kappa}B$ at the downstream of MAVS, while it is not involved in IRF3 nuclear translocation. Mechanical studies showed that TRIM25 is involved in TRAF6-mediated NF-${\kappa}B$ activation. These collectively indicate that TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-${\kappa}B$.

• 한국대기환경학회지
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• 제21권5호
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• pp.525-535
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• 2005

Using a three-dimensional computational fluid dynamics (CFD) model with the $k-{\varepsilon}$ turbulence closure scheme based on the renormalization group theory, flow regimes in urban street canyons are classified according to the building and street aspect ratios. The transition between skimming flow (SF) and wake interference flow (WIF) is determined with the size of double-eddy circulation generated behind the upwind building. The transition between WIF and isolated roughness flow (IRF) is determined with the flow reattachment distance from the upwind building. The critical aspect ratios at which the flow transition occurs are found and compared with those in previous studies. The results show that the flow-regime classification method used in this study is quite reasonable and that the values of the critical aspect ratios are generally consistent with those in fluid experiments or large-eddy simulation. The regression equation describing a relation between the building and street aspect ratios at the flow-regime transition is presented.