• Title/Summary/Keyword: IR36

Search Result 310, Processing Time 0.021 seconds

Improvement of Cultural Efficiency Using DNA Markers in Anther and Seed Culture of Rice (DNA marker를 이용한 벼의 조직배양 효율 재선)

  • Kim Kyung-Min
    • Journal of Life Science
    • /
    • v.16 no.3 s.76
    • /
    • pp.527-533
    • /
    • 2006
  • The purpose of this study is to improve the culturability of a indica type rice cultivar, IR 36, using DNA marker associated with the ability of plant regeneration in anther and seed culture. The varietal difference of ability of callus formation and plant regeneration was investigated in anther and seed culture of 8 rice cultivars. Three japonica rice cultivars showed to have better culturability than those of tongil and indica type genotypes. But two indica/japonica lines, 'MGRI 079' and 'MGRI 036', which were selected to have good culturability in previous study showed the highest regenerability (20%) in anther culture of 8 rice cultivars. Thirty four $BC_2F_4$ lines were selected by marker screening using RZ400 for 100 $BC_2F_4$ lines derived from a cross $'MGRI\;079/IR\;36^{^*3}'$. The frequency of callus formation of 30 $BC_2F_4$ lines was higher than those of 'IR 36' in anther culture of the selected $BC_2F_4$ lines. The ability of plant regeneration of 15 lines was higher than that of 'IR 36' in the seed culture of 34 $BC_2F_4$ lines. A promising line, $BC_2F_4-28$, was selected to have better culturability in the anther and seed culture of the $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4-28$ was similar to 'IR 36'. In seed culture of 50 $BC_4F_3$ lines derived from a rice cross $'MGRI\;079/IR\;36^{^*5}'$, 11 lines including $BC_4F_3-3$ showed to have higher regenerability compared with 'IR 36'. The highest frequency of plant regeneration (11%) was obtained from $BC_4F_3-46$ in seed culture of the $BC_4F_3$ lines.

Effect of Protease and Disulfide Bond Reducing Agent Treatment on the Physicochemical and Gelatinization Properties of Rice (단백질 가수분해 효소 및 이황화 결합 환원제 처리가 쌀의 이화학적 성질과 호화 특성에 미치는 영향)

  • Kim, Sung-Ran;Ahn, Seung-Yo
    • Applied Biological Chemistry
    • /
    • v.38 no.6
    • /
    • pp.554-562
    • /
    • 1995
  • Effect of the modification of rice protein by protease and 2-mercaptoethanol on physicochemical and gelatinization properties was investigated for the three cultuvars of rice. Total amylose contents of Chucheongbyeo(japonica type), Chosengtongilbyeo(tongil type, indica x japonica) and IR 36 (indica type) were in the range of $20{\sim}25%$. Total amylose and insoluble amylose content of IR 36 were higher than the others. The differential scanning calorimetric and X-ray diffraction results revealed higher relative crystallinity of IR 36 than the others. Water uptake was increased and amylographic viscosity was significantly decreased by 2-mercaptoethanol treatment. Amylographic viscosity was significantly decreased and hot water-soluble carbohydrate content was increased by protease treatment. The proportions of high molecular weight of soluble carbohydrate fractionated by gel filtration chromatography were increased by protease treatment. These effects were most significant in IR 36. This results suggested that starch gelatinization be accelerated by alteration of the protein with protease and reducing agent.

  • PDF

Improvement of cultural efficiency using DNA markers in anther and seed culture of rice (DNA marker를 이용한 벼의 조직배양 효율개선)

  • Kim, Hong-Jib;Kim, Tae-Heon;Sohn, Jae-Keun
    • Current Research on Agriculture and Life Sciences
    • /
    • v.27
    • /
    • pp.21-28
    • /
    • 2009
  • The purpose of this study was to improve the culturability of 'IR 36', a indica type rice cultivar using DNA marker associated with the ability of plant regeneration in anther and seed culture. The culturability of 6 rice cultivars and 2 indica/japonica lines ('MGRI 036', 'MGRI 079') were investigated in anther and seed culture. The culturability of 3 japonica rice cultivars were much higher than tongil and indica rice cultivars, and 'MGRI 036' and 'MGRI 079' has high culturability with 20% regenerability, also. 34 $BC_2F_4$ 4 lines were selected by marker screening using RZ400 among 90 $BC_2F_4$ lines derived from a cross between 'MGRI 079' and 'IR 36'. The frequency of callus formation of 10 $BC_2F_4$ lines were higher than 'IR 36' in anther culture among selected 34 $BC_2F_4$ lines. The ability of plant regeneration of 10 lines were higher than 'IR 36' in the seed culture among selected 34 $BC_2F_4$ lines. A promising line, $BC_2F_4$-28, was selected to have better culturability in the anther and seed culture among selected 34 $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4$-28 was similar to 'IR 36'. Using the RZ400 DNA marker associated with the culturability will be useful method for improving of indica rice culticvar's culturability in rice breeding program.

  • PDF

Temperature Compensation of Nondispersive Infrared Gas Senor: Infrared Light Absorbance (비분산 적외선 가스 센서 온도 보상법: 적외선 흡수도)

  • Yi, SeungHwan
    • Journal of Sensor Science and Technology
    • /
    • v.30 no.1
    • /
    • pp.36-41
    • /
    • 2021
  • The motivation of this paper is to easily analyze the properties of nondispersive infrared gas sensor that has more than two different optical path length and to suggest the criterion and definition of infrared light absorbance in order to minimize the measurement errors. With the output voltage ratios and the normalized derivatives of infrared ray (IR) absorbance, when the normalized derivatives of IR absorbance decreases from 0.28 to 0.10, the lower and higher limits of errors were decreased from -5.62% and 2.39% to -4.27% and 2.78%. When the normalized derivatives of IR absorbance were 0.10, the output voltage could be partitioned into two regions with one exponential equation and the temperature compensation error was less than 5%.

Studies on the Effect of Temperature During the Reduction Division and the Grain Filling Stage in Rice Plants II. Effect of Air Temperature at the Grain Filling Stage in Indica-Japonica Crosses (수도의 감수분열기 및 등숙기에 있어서 온도반응에 관한 연구 제2보 수도 Indica$\times$Japonica 품종의 등숙기에 있어서의 온도반응)

  • kyu-Chin Kim
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.28 no.1
    • /
    • pp.58-75
    • /
    • 1983
  • The effects of air temperature on the grain filling stage of indica-japonica crosses were studied in artificial temperature-controlled cabinets, using Indica-Japonica crosses (Suweon 264, Suweon 258 and Milyang 29), Indica IR 36 and Lengkwang) and a Japonica rice (Jinheung). The optimum temperature to achieve maximum grain weight during the grain-filling stage was $26/18^{\circ}C$ for all varieties. Within the temperature range of 13 to $28^{\circ}C$, the grain filling period was shorter as the temperature was increased. At the highest temperature $(32/24^{\circ}C)$ the upper-position grains ("special spikelets") of IR 36 required 13days after flowering to reach the maximum weight, in Jinheung 23 days, in indica-japonica varieties (Suweon 264, Suweon 258, and MiIyang 29) 18 days, and in Lengkwang, 23 days. In the case of Lengkwang, at $32/24, \;29/21^{\circ}C$ and $26/18^{\circ}C$temperature range the upper-position grain also required same 23 days after flowering to reach the maximum weight. At the lowest temperature range of $17/9^{\circ}C$, Jinheung required 68 days and Lengkwang 53 days after flowering to reach maximum grain weight. The whole panicle took 10 to 15 days later than the special spikelets to reach the maximum weight. At lower-than-optimum temperature range $(l7/9^{\circ}C\;and\;20/12^{\circ}C)$, all IR 36 plant died within 15 days after flowering. In the case of indica-japonica varieties all plants died within 15 days only at $17/9^{\circ}C$. There were more chalky grains when the temperature was higher and lower than optimum. The highest and lowest temperature range produced dead rice (black and dark red rice). Lower than optimum temperature ranges $(17/9^{\circ}C\;and\;20/12^{\circ}C)$ affected grain quality (length, width and depth) at these temperature ranges, grains were shorter, narrower, and thinner than grains at the optimum temperature of $26/18^{\circ}C$. With regared and the effects of temperature on frequency of grain weight at grain filling stage of indica variety IR 36, the highest $(32/24^{\circ}C)$ and lowest $(23/15^{\circ}C)$ temperature ranges matched the frequency of grain weight curves. In Japonica variety Jinheung, at the three temperature ranges, that is, lowest $(17/9^{\circ}C)$ highest $(32/24^{\circ}C)$ and optimum $(26/18^{\circ}C)$, the frequency of grain weight curve showed very different patterns, In the case of indica-japonica variety Suweon 258, the frequency of grain weight curve was midway between that of IR 36 and Jinheung. Jinheung.

  • PDF

Effect of Planting Methods on Lodging Reaction in Lowland Rice (재배방법(裁培方法)이 벼 도복(倒伏)에 미치는 영향(影響))

  • Lee, Sang Chul;De Datta, S.K.
    • Current Research on Agriculture and Life Sciences
    • /
    • v.7
    • /
    • pp.11-21
    • /
    • 1989
  • This experiment was conducted at the IRRI experimental fram, Los Banos, Philippines to determine the effects of planting methods on lodging in rice, and to determine plant characteristics that induce lodging. Moderately lodging-susceptible IR36, lodging-susceptible line IR21820-3-2-2-3 and lodging-resistant IR8 and IR64 were tested. Except in IR8, lodging resistance of all test rices was higher when transplanted than when broadcast-seeded. Transplanted rices yielded higher than did broadcast-seeded rices except with broadcast-seeded IR8. Yield reduction due to lodging varied from 0.6 to 1.5 t/ha. Yield components and vegetative characteristics did not differ significantly between planting methods. However, higher culm K content was observed in transplanted rice, and it consequently increased lodging resistance. Phosphorus ans Si contents did not significantly affect culm strength.

  • PDF

Assessment of gene flow from insect-resistant genetically modified rice (Agb0101) to non-GM rice (해충저항성 유전자변형 벼(Agb0101) 유전자 이동성 평가)

  • Oh, Sung-Dug;Yun, Doh-Won;Sohn, Soo-In;Park, Soon Ki;Chang, Ancheol
    • Korean Journal of Breeding Science
    • /
    • v.49 no.3
    • /
    • pp.180-189
    • /
    • 2017
  • Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-GM rice (Nakdongbyeo), indica non-GM rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-GM rice and out-crossing ranged from 0.0005% at IR36 to 0.0027% at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow.

Low-iridium Doped Single-crystalline Hydrogenated Titanates (H2Ti3O7) with Large Exposed {100} Facets for Enhanced Oxygen Evolution Reaction under Acidic Conditions ({100} 단결정 수소화 티타네이트(H2Ti3O7)를 활용한 저함량 Irridium 수전해 양극 촉매 개발)

  • Sun Young Jung;HyukSu Han
    • Journal of the Microelectronics and Packaging Society
    • /
    • v.30 no.1
    • /
    • pp.79-89
    • /
    • 2023
  • Development of efficient and stable electrocatalysts for oxygen evolution reaction (OER) under acidic conditions is desirable goal for commercializing proton exchange membrane (PEM) water electroyzer. Herein, we report iridium-doped hydrogenated titanate (Ir-HTO) nanobelts as a promising catalyst with a low-Ir content for the acidic OER. Addition of low-Ir (~ 3.36 at%) into the single-crystalline HTO nanobelts with large exposed {100} facets significantly boost catalytic activity and stability for OER under acidic conditions. The Ir-HTO outperforms the commenrcial benchmark IrO2 catalyst; an overpotential for delivering 10 mA cm-2 current density was reduced to about 25% for the Ir-HTO. Moreover, the catalytic performance of Ir-HTO is positioned as the most efficient electrocatalyst for the acidic OER. An improved intrinsic catalytic activity and stability are also confirmed for the Ir-HTO through in-depth electrochemical characterizations. Therefore, our results suggest that low-Ir doped single-crystalline HTO nanobelts can be a promising catalyst for efficient and durable OER under acidic conditions.

Cyclovoltametric Methods for the Ionization Potential and Electron Affinity of Iridium ppy Derivatives

  • Shin, Dong-Myung;Sohn, Byoung-Chung
    • Journal of the Korean Applied Science and Technology
    • /
    • v.20 no.2
    • /
    • pp.125-129
    • /
    • 2003
  • The effects of molecular structure on the redox properties of the organic electroluminescent materials (Ir$(ppy)_3$ Ir$(m-ppy)_3$ Ir$(p-toly)_3$) were studied using cyclic voltammetry and spectroscopy. These iridium complexes show reversible oxidation and reduction on the electrode, which produce the symmetric cyclic voltammogram. It indicates that these materials are very stable under repetitive oxidation/reduction cycles. The electrochemically determined ionization potentia/electron affinity values are 5.4OeV/3.02eV for Ir$(ppy)_3$, 5.36eV/2.96eV for Ir$(m-ppy)_3$, and 5.35eV/2.97eV for Ir$(p-toly)_3$ from the SCE(Standard Calomel Electrode). The electrically determined band gaps are 2.38eV (521nm), Ir$(ppy)_3$, 2.4OeV (517nm), Ir$(m-ppy)_3$, and 2.38eV (521nm). Ir$(p-toly)_3$, which are similar with the optical band gaps. The position of methyl group on 2-phenylpyridine (ppy) effects do not influence much on the ionization potential, electron affinity, and band gap of Ir$(ppy)_3$ derivatives.

Variation of Yields and Growth-related Characteristics Shown by Different Ecotype of Rice Varieties in the Temperate and Tropical Zones II. Yields and Yield-related Characteristics Variation Shown by Varieties in Temperate and Tropical Zones (온대와 열대에서 생태형이 다른 수도품종의 수량 및 생육형질의 변이 II. 온대와 열대지방간에 품종별 수량 및 수량형질의 변이)

  • ;Eun-Woong Lee
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.31 no.4
    • /
    • pp.391-402
    • /
    • 1986
  • A total of 16 varieties from Korea and Indonesia were tested in Suwon, Korea (126$^{\circ}$9'E, 37$^{\circ}$16'N, 37m amsl) and Bali, Indonesia (115$^{\circ}$ 14'E, 8$^{\circ}$42' and 10m amsl). Most varieties of Indica and India/Japonica types showed adaptability to both areas. Generally Indica-type showed more number of panicles per hill than that of Indica/Japonica, while Indica/Japonica showed better performance in spike let number per panicle and 1,000 grain weight than Indica-type varieties.

  • PDF