• Journal of Positioning, Navigation, and Timing
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• 제11권1호
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• pp.29-34
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• 2022

In this paper, we analyze the Non-Holonomic Constraint (NHC) satisfaction of Inertial Navigation System (INS) for vehicular navigation according to Inertial Measurement Unit (IMU) location. In INS-based vehicle navigation, NHC information is widely used to improve INS performance. That is, the error of the INS can be compensated under the condition that the velocity in the body coordinate system of the vehicle occurs only in the forward direction. In this case, the condition that the vehicle's wheels do not slip and the vehicle rotates with the center of the IMU must be satisfied. However, the rotation of the vehicle is rotated by the steering wheel which is controlled based on the Ackermann geometry, where the center of rotation of the vehicle exists outside the vehicle. Due to this, a phenomenon occurs that the NHC is not satisfied depending on the mounting position of the IMU. In this paper, we analyze this problem based on Ackermann geometry and prove the analysis result based on simulation.

• 생명과학회지
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• 제31권11호
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• pp.969-979
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• 2021

당뇨병은 만성대사성 질환으로 우리나라 국민의 사망요인 중 5위를 차지하고 있다. 제 2형 당뇨병에서 나타나는 인슐린 분비 감소는 베타세포의 자가사멸에 의한 베타세포질량의 급격한 감소로 인한 것으로 보고되고 있으며, 베타세포의 자가사멸을 촉진하는 요인으로 고혈당에 의한 당독성 및 활성산소종들의 증강에 의한 산화스트레스 등이다. 벼메뚜기 추출물은 고농도 포도당 처리된 INS-1 췌장 베타 세포에서 세포 생존율을 증가시키고, 지질과산화물, 세포 내 ROS 및 NO 수준을 감소시켰다. 세포사멸 관련 인자의 유전자 발현 결과 pro-세포자가사멸인자인 Bax, Cytochrome C, caspase-3 및 caspase-9의 단백질 발현을 유의적으로 감소시켰고, anti-세포자가사멸인자인 Bcl-2 발현을 증가시켰다. Annexin V/I propidium iodide 염색법을 통하여 벼메뚜기 추출물이 고농도 포도당으로 유도된 세포 사멸을 감소시키고, INS-1 췌장 베타세포에서의 인슐린 분비능을 증가시키는 것으로 사료된다. 그러므로 벼메뚜기 추출물이 고농도 포도당으로 손상된 INS-1 췌장 베타세포의 보호효과를 나타낸다. 본 연구의 결과는 벼메뚜기 추출물이 당뇨병 치료에 도움을 주는 항당뇨 기능성 식품 소재 및 개발에 기여할 수 있음을 시사한다.

• The Korean Journal of Physiology
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• 제29권2호
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• pp.269-277
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• 1995

Membrane vesicles were prepared by differential centrifugation from epithelial cells of porcine trachea. Total activity of microsomal ATPases was measured spectrophotometrically by a coupled enzyme assay. The steady-state activity of the enzyme was $329{\pm}10$ nmol/min mg protein. Thapsigargin, a specific antagonist of intracellular $Ca^{2+}-ATPase$, inhibited about 50% of the activity, leaving $178{\pm}18\;nmol/min .mg$ protein (n=6), indicating that the $Ca^{2+}-ATPase$ is one of the major microsomal ATPases. The microsomes used in this study appeared to be tight-sealed vesicles since they showed saturation in $^{45}Ca^{2+}$ uptake experiments. Inositol 1,4,5-trisphosphate $InsP_{3}, 4\;{\mu}M$, an agonist of $InsP_{3}$-sensitive $Ca^{2+}$ release channel ($InsP_{3}$, receptor), and Ca-ionophore A23187 $(10\;{\mu}M)$ induced $^{45}Ca^{2+}$ releases of 20% and 50% of stored $^{45}Ca^{2+}$, respectively. The addition of $(10\;{\mu}M\;InsP_{3}$ also increased the microsomal ATPase activity from $282{\pm}8$ nmol/min mg protein to $334{\pm}21$ nmol/min . mg protein in the intact vesicles. Similar increase in the activity was observed by making microsomes leaky (uncoupling) using the Ca-ionophore A23187. ;$InsP_{3}-induced$ effects were blocked by either thapsigargin or heparin suggesting that: 1) the $InsP_{3}-induced$ increase in ATPase activity is mediated by microsomal $Ca^{2+}-ATPase$, and 2) dissipation of $Ca^{2+}$ gradient across the microsomal membrane is responsible for the $InsP_{3}-induced$ effect. In order to test the dependence of the $Ca^{2+}-ATPase$ activity on the activity of $InsP_{3}-induced$ the activity of ATPases was monitored in various concentrations of free $Ca^{2+}$ using $EGTA-Ca^{2+}$ buffers. The $Ca^{2+}$-dependent biphasic change is the well-known character of $InsP_{3} receptor but not of microsomal $Ca^{2+}-ATPase$ in non-excitable cells; however, the activity of microsomal ATPase appeared biphasic and a maxim진 activity of $397{\pm}36nmol/min\;.mg$ protein was obtained in the solution containing 100 nM free $Ca^{2+}$. Below or above this concentration, the activity of ATPases was lower. These results strongly support a positive correlation of microsomal $Ca^{2+}-ATPase$ to the $InsP_{3}$ receptors in epithelial microsomes.

• Food Science and Biotechnology
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• 제17권5호
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• pp.1122-1127
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• 2008

A high dietary oxalate intake may lead to calcium oxalate (CaOx) kidney stones in the gastrointestinal tract. Most soy foods contain high concentrations of oxalate and/or phytate. This study analyzed the changes in oxalate (Ox), phytate ($InsP_6$), and calcium (Ca) during soymilk processing from the seeds of Korean recommended soybean cultivars (cvs). The contents of Ox, $InsP_6$, and Ca in 21 cvs ranged from 14, 108, and 148 to 231, 279, and 246 mg/100 g of dry seed, respectively. Seven cultivars were selected from the 21 cvs by the distributions of Ox, $InsP_6$, and Ca. Then, each contents of soymilk prepared from the 7 cvs were determined. All contents were lower in soymilk than in seeds, but the Ox to $InsP_6$ ratios changed from varying ratios (0.1-0.8) to normal ratios (0.8-1.0) in all cvs except 'Paldalkong'. Consequently, during soymilk processing, the Ox content was decreased and the $InsP_6$ content was remained higher than the Ox content although the Ox was likely to be less reductive than the $InsP_6$. These results may provide better information for minimizing the risk of formation of Ox kidney stones due to consumption of soy products.

• 전자공학회논문지SC
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• 제41권1호
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• pp.1-8
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• 2004

INS는 위치, 속도 및 자세정보를 고속으로 제공하며 스스로 항법정보를 제공하는 장점이 있으나 오차가 시간에 따라 증가하는 단점이 있다. 반면에 GPS는 데이터를 저속으로 제공하며 재밍(Jamming)에 영향을 쉽게 받으나 오차가 시간에 상관없이 일정하다는 장점이 있다. 따라서 상호보완적인 특성을 가지는 INS와 GPS를 통합하였을 때 더욱더 정확한 항법해를 제공할 수 있다. 그러나 INS와 GPS를 통합하는 과정에서 INS정보와 GPS정보의 정확한 시각적 동기가 어려우며, 시각적 비동기에 의한 오차는 일정한 측정오차를 유발하며 고속으로 운항하는 경우에는 큰 영향을 미치게 된다. 본 연구는 GPS/INS 통합항법 시스템에서 측정치 시간지연에 의해 유발되는 오차를 효과적으로 줄이기 위해 바이어스 분리형 칼만필터를 이용한 시간지연오차 보상기법을 강결합 방식과 약결합 방식에 대하여 각각 제안하였다. 각각의 통합모델에서 위치와 속도보정의 측정방정식을 지연시간에 관하여 선형화하였고 지연 상태변수를 기존의 선형 오차방정식에 추가하고 바이어스 분리형 칼만필터를 적용하여 시간지연을 초기에 추정하여 보상하도록 구성하였으며 시뮬레이션을 통하여 성능을 검증하였다.

• Nutrition Research and Practice
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• 제12권3호
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• pp.183-190
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• 2018

BACKGROUND/OBJECTIVE: This study was designed to investigate how a Portulaca oleracea L. extract (POE) stimulates insulin secretion in INS-1 pancreatic ${\beta}-cells$. MATERIALS/METHOD: INS-1 pancreatic ${\beta}-cells$ were incubated in the presence of various glucose concentrations: 1.1 or 5.6, 16.7 mM glucose. The cells were treated with insulin secretagogues or insulin secretion inhibitor for insulin secretion assay using an insulin ELISA kit. In order to quantify intracellular influx of $Ca^{2+}$ caused by POE treatment, the effect of POE on intracellular $Ca^{2+}$ in INS-1 pancreatic ${\beta}-cells$ was examined using Fluo-2 AM dye. RESULTS: POE at 10 to $200{\mu}g/mL$ significantly increased insulin secretion dose-dependently as compared to the control. Experiments at three glucose concentrations (1.1, 5.6, and 16.7 mM) confirmed that POE significantly stimulated insulin secretion on its own as well as in a glucose-dependent manner. POE also exerted synergistic effects on insulin secretion with secretagogues, such as L-alanine, 3-isobutyl-1-methylxanthine, and especially tolbutamide, and at a depolarizing concentration of KCl. The insulin secretion caused by POE was significantly attenuated by treatment with diazoxide, an opener of the $K{^+}_{ATP}$ channel (blocking insulin secretion) and by verapamil (a $Ca^{2+}$ channel blocker). The insulinotropic effect of POE was not observed under $Ca^{2+}$-free conditions in INS-1 pancreatic ${\beta}-cells$. When the cells were preincubated with a $Ca^{2+}$ fluorescent dye, Fluo-2 (acetoxymethyl ester), the cells treated with POE showed changes in fluorescence in red, green, and blue tones, indicating a significant increase in intracellular $Ca^{2+}$, which closely correlated with increases in the levels of insulin secretion. CONCLUSIONS: These findings indicate that POE stimulates insulin secretion via a $K{^+}_{ATP}$ channel-dependent pathway in INS-1 pancreatic ${\beta}-cells$.

• Nutrition Research and Practice
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• 제8권5호
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• pp.494-500
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• 2014

BACKGROUND/OBJECTIVES: This study investigated whether Padina arborescens extract (PAE) protects INS-1 pancreatic ${\beta}$ cells against glucotoxicity-induced apoptosis. MATERIALS/METHODS: Assays, including cell viability, lipid peroxidation, generation of intracellular ROS, NO production, antioxidant enzyme activity and insulin secretion, were conducted. The expressions of Bax, Bcl-2, and caspase-3 proteins in INS-1 cells were evaluated by western blot analysis, and apoptosis/necrosis induced by high glucose was determined by analysis of FITC-Annexin V/PI staining. RESULTS: Treatment with high concentrations of glucose induced INS-1 cell death, but PAE at concentrations of 25, 50 or $100{\mu}g/ml$ significantly increased cell viability. The treatment with PAE dose dependently reduced the lipid peroxidation and increased the activities of antioxidant enzymes reduced by 30 mM glucose, while intracellular ROS levels increased under conditions of 30 mM glucose. PAE treatment improved the secretory responsiveness following stimulation with glucose. The results also demonstrated that glucotoxicity-induced apoptosis is associated with modulation of the Bax/Bcl-2 ratio. When INS-1 cells were stained with Annexin V/PI, we found that PAE reduced apoptosis by glucotoxicity. CONCLUSIONS: In conclusion, the present study indicates that PAE protects against high glucose-induced apoptosis in pancreatic ${\beta}$ cells by reducing oxidative stress.

• 대한공간정보학회지
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• 제17권1호
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• pp.71-78
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• 2009

GPS/INS를 이용한 항공사진측량의 경우 GPS 이동측위를 위해 촬영지역에 GPS 지상기준국의 설치가 필수적이다. 하지만 항공사진측량을 위한 지상기준국의 설치는 여러 가지 제한 요소들로 인해 현실적으로 어려운 경우가 많다. 이러한 문제점을 해결하기 위해 본 연구에서는 상시관측소 자료를 이동측위에 이용하여도 충분한 정확도를 확보할 수 있는 지를 분석하였다. 30초 간격으로 저장되어 있는 상시관측소 자료의 내삽을 통해 결정한 외부표정요소와 촬영지역에서 수신된 지상기준국 자료로부터 결정한 외부표정요소를 GPS/INS 항공삼각측량에 각각 적용한 후 정확도를 비교하였다. 그 결과 상시관측소 자료로 지상기준국 자료를 대체하여 사용하여도 충분한 정확도를 얻을 수 있었다.

• 한국육종학회지
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• 제40권3호
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• pp.336-339
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• 2008

A new spray chrysanthemum cultivar, 'Moon Festival' was released by National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Korea in 2007. A cross was made in 2002 between '01B1-559', a breeding line of NHRI and 'Baeksokuk', a spray cultivar with white petals. Trials were conducted from 2005 to 2007 for evaluation and selection of this cultivar, including shading culture in summer and retarding culture in autumn. The natural flowering time of "Moon Festival" is late October, but year-round flowering is possible by shading and lighting treatment. The cultivar is semi- double type flowers with light-ivory white petals and green flower center. Flower neck and stem are very hardy. The diameter of flower is 67.5 mm. The number of flowers per stem and petals per flower is 11.5 and 100.5, respectively. The days to flowering under the short day is about 54 in autumn season.

• 한국항공우주학회지
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• 제48권11호
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• pp.873-880
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• 2020

본 논문은 스트랩다운 탐색기 측정치와 INS 정보를 이용하여 시선각속도를 추정하는 유도필터 설계에 대해서 다룬다. 제안하는 유도필터는 탐색기 측정치와 유도탄 자세로부터 획득 가능한 시선각과 표적의 위치와 유도탄과의 상대 위치를 측정치로 하고 있으며, 주기 및 동기가 맞지 않는 두 센서의 출력을 사용하기 위해 비동기 필터를 기반으로 하고 있다. 제안한 방법을 통해 시간지연이 큰 탐색기 측정치를 사용함으로써 생길 수 있는 기생루프에 대한 영향을 줄이고 추정성능을 향상시킬 수 있다.