• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.362-369
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• 2013

Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity ($H_T$) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles ($N_E$) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei's genetic diversity (H) mean value was 0.218, and Shannon's information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei's genetic diversity, and Shannon's information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.

• Journal of Korean Society on Water Environment
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• v.34 no.5
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• pp.514-536
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• 2018

GPI (Group Pollution Index) using 29 indicator groups of Korean benthic macroinvertebrates was proposed in 1992, a higher category taxa-level index developed for rapid field assessment of organic water pollution. This study was performed to revise the assessment scheme of GPI based on taxonomic performance and ecological information accumulated since 1992. The original GPI was renamed SBMI (Simple Benthic Macroinvertebrates Index), and SBMI was based on saprobic valency of 26 indicator groups composed of higher category taxa (mainly family ~ phylum) excluding some genus or species-level taxa. SBMI revealed highly significant correlation with concentration of 5-day biochemical oxygen demand ($BOD_5$) (correlation coefficient r = 0.78, n = 569 sites), total suspended solids (r = 0.69), and total phosphorus (r = 0.77). Also, SBMI revealed strong correlation with Shannon-Weaver's species diversity (r = -0.85), Margalef's species richness (r = -0.85), and McNaughton's dominance (r = 0.83). Determination coefficient of SBMI to concentration of water quality items and values of community indices such as species diversity was 3 ~ 8 % and approximately 11 ~ 14 % higher than that of GPI, respectively. Correlation between SBMI and water quality factors or community indices such as species diversity did not reveal much difference compared to that of species-level indices, such as BMI (Benthic Macroinvertebrates Index) and ESB (Ecological Score of Benthic Macroinvertebrates). SMBI is a simple-qualitative index with higher category taxa easily identified, and is applicable for rapid field assessment of water environment impairment.

• Journal of Korean Society of Forest Science
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• v.99 no.5
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• pp.736-743
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• 2010

This study examined the ecological attributes of classified forest community by cluster analysis in the mixed forest of Mt. Seokbyung Experimental Forest of Gangwon-Do. The vegetation data were collected in randomly established 51 sample plots (2.04 ha) and analysis adopted the cluster analysis, importance value index, and Shannon's diversity index. Main results were as follows; 1) the study area was classified into 4 clusters (A, B, C and D). 2) The cluster A was dominated by Pinus densiflora with an importance value of 71.6%. The most dominant species in the cluster B and cluster C were Larix leptolepis (57.1%) and Quercus mongolica (40.2%), respectively. Finally, The cluster D was dominated by P. densiflora (30.6%) and Q. mongolica (31.0%) with the mixed forest. 3) In the P. densiflora community (cluster A), distribution of DBH class showed a reverse J-shaped curve. In the L. leptolepis community (cluster B), individuals of dominant species had the bell-shaped distribution. Oak species indicated uniform distribution of DBH class (under 25 cm) in the mixed P. densiflora - Q. mongolica community (cluster D). 4) The species diversity index of the communities in descending order were: Pinus densiflora - Q. mongolica community > Larix leptolepis community > Pinus densiflora community > Quercus mongolica community.

• Journal of Korean Society of Forest Science
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• v.107 no.2
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• pp.151-157
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• 2018

This study was to analyze the genetic diversity of Quercus gilva Blume growing in Jeju Island for developing a preservation strategy. We examined the genetic diversity and structure using 6 ISSR primers and investigated 67 polymorphic ISSR amplicons in 80 trees distributed among five populations. The average of proportion of polymorphic loci were 93%, the average level of Shannon's information index was 0.237, and Nei's genetic diversity was 0.156. According to the analysis of the molecular variance (AMOVA), $F_{st}$ was 0.169 indicating there was a genetic variation among five populations. 17% of the total variation was allocated among the five populations, while the other 83% of the total variation was in individual trees in each population. The result could be due to the uneven number of trees among the five populations. Based on these results, the preservation strategy could be developed, for examples, considering for designation as "forest genetic resources conservation area" about the habitat, monitoring continuously, fostering the growth of seedling, ex situ preservation of genetic resources, and comparing the differences of environmental and genetic characteristic with population in ex situ.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.723-728
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• 2006

Since the forest succession is changing process in the structure and function of a forest for the long period of time, the objectivity of the process could be improved through the development of model or statistical methodology. This study was carried out to estimate succession index based on climax index and species composition parameters, thereupon to compare the succession index with life form and species diversity so as to grasp the information of quantitative successional process for the six community types in the natural deciduous forest of Mt. Jumbong area. The results indicated that Quercus mongolica-Tilia amurensis community had the greatest succession index of 67.5, followed by Abies holophylla-Fraxinus mandshurica community with 67.4. Juglans mandshurica-Cornus controversa community was recorded the smallest value of 60.5. The succession index of overstory tended to increase considerably at the stage of steady state for all community types except Quercus mongolica-Tilia amurensis community. However, The index of midstory hardly varied as the forest succession would progress for all community types. The succession index was negatively correlated to wind-water dispersion type of life form and positively to animal dispersion type at the 5% probability level. On the other hand, the index was not significantly correlated to the species richness and Shannon's species diversity index.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.450-461
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• 1998

To investigate the plant community structure of Paekdam-Valley in Soraksan National Park, fifty-two plots have been set up and surveyed. By DCA ordination technique, the plant communities were classified into fkve community types, which were Pinus densiflora community and Q. mongolica community. It was supposed that Q. serrata community change to C. laxiflora community. It seemed that P. densiflora community, deciduous broad-leaved mixed forest and Q. serrata community would not be replace by another woody species. And it seemed that C. laxiflora community succeeded to Q. serrata community, so it was similar to the typical ecological succession of natural forest in the middle temperate regions. The range of Shannon's diversity index was 0.9827~1.2946(Unit area : 400m$^2$) and that was over another Korean National Parks area relatively.

• Korean Journal of Medicinal Crop Science
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• v.20 no.6
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• pp.440-446
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• 2012

Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon's information Index (S.I.) indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.310-319
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• 2013

The genetic diversity and structure of eight populations of Coreanomecon hylomeconoides Nakai, an endemic Korean plant, were investigated using 50 ISSR loci from eight primers. The average percentage of polymorphic loci was 47.3%. The Shannon's index (SI=0.218) and gene diversity (h=0.142) were relatively lower than those of other long-lived perennials. The Sancheong (SI=0.233, h=0153), Gwangyang (SI=0.263, h=0.171), and Suncheon (SI=0.241, h=0.159) populations showed greater genetic diversity than the Namhae and Gwangju populations, which are on the edge of the distribution. Analysis of molecular variance (AMOVA) showed that 18% of the total variation could be attributed to differences among populations, and 82% to differences within populations, indicating moderate gene flow among adjacent populations. These results were supported by value of Nm (2.184). The UPGMA conducted using the genetic distance and Bayesian cluster analysis showed a remarkable geographic trend structured into east and west regions. Overall, the results indicate that the Sancheong and Gwangyang populations, which had a large population size and higher degree of genetic diversity, should be the focus of in situ conservation.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.188-199
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• 2006

The purpose of this study was to provide data for conservation management of Pinus densiflora forest by analyzing ecological characteristics and the change of Pinus densiflora community structure for fifteen years in Gayasan National Park. According to the results of TWINSPAN analysis of Pinus densiflora forest, there were twenty plots(unit: $500m^2$)and it was classified into seven community types. The dominant species were Pinus densiflora in canopy layer, Carpinus laxiflora, Quercus variabilis, Quercus serrata in the understory layer and Sasa borealis, Rhododendron mucronulatum, Rhus trichocarpa in the shrub layer. The index of shannon's diversity was from 0.6803 to 1.2559 per $500m^2$ and the range of species number was from twenty-seven to fourty. As a result of the change of Pinus densiflora forest structure, the importance percentage(I.P.) of Carpinus laxiflora, Quercus variabilis, Quercus serrata in the understory layer were increased for fifteen years. The index of shannon's diversity changed $0.2608{\sim}1.0124\;into\;0.5547{\sim}1.2567\;per\;500m^2$ and species number changed $14{\sim}26\;into\;26{\sim}34$ for fifteen year.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.672-677
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• 2005

The standard reference collection of strains for E. coli, the ECOR collection, was analyzed by a genome-based typing method. Seventy-one ECOR strains were subjected to repetitive extragenic palindromic PCR genome fingerprinting with BOX primers (BOX-PCR). Using a similarity value of 0.8 or more after cluster analysis of BOX-PCR fingerprinting patterns to define the same genotypes, we identified 28 genotypes in the ECOR collection. Shannon's entropy-based diversity index was 3.07, and the incident-based coverage estimator indicated potentially 420 genotypes among E. coli populations. Chi-square test of goodness-of-fit showed statistically significant association between the genotypes defined by BOX-PCR fingerprinting and the groups previously defined by multi-locus enzyme electrophoresis. This study suggests that the diversification of E. coli strains in natural populations is actively ongoing, and rep-PCR fingerprinting is a convenient and reliable method to type E. coli strains for the purposes ranging from ecology to quarantine.ine.