The regional distributions and relative frequencies of endocrine cells were studied histochemically (Grimelius and Masson-Hamperl (M-H) silver methods) in the alimentary tract of the red-eared slider, Trachemys scripta elegans. Samples were taken from the esophagus, fundus, pylorus, duodenum, jejunum, ileum and large intestine. Argyrophil (Grimelius-positive) cells and argentaffin (M-H-positive) cells were found in the whole alimentary tract in this study. Spherical to spindle and/or oval to round-shaped argyrophil or argentaffin cells were located in the gastric glands of the stomach regions, in the basal portion of the epithelium of intestinal tract or the esophagus with variable frequencies. Argentaffin cells were more numerously detected in the whole alimentary tract compared with those of argyrophil cells in this study. Argyrophil cells were observed in the whole alimentary tract including the esophagus and the most predominant region was the rectum with moderate frequency. The relative frequency of these cells was rare in the esophagus, fundus, duodenum, jejunum and ileum, respectively and a few frequency in the pylorus. Argentaffin cells were also observed in the whole alimentary tract including the esophagus and the most numerously demonstrated region was the rectum with numerous frequency. They were observed with a few frequencies in the remaining regions of the alimentary tract except for the rectum, respectively. However, to know the exact type of the argyrophil cells and argentaffin cells that were observed in this study, more developmental methods such as immunohistochemistry were needed.
Sledge net samples were taken over the neap and spring tide cycles in January 1993 from the bottom and surface of 1 m depth and at the water's edge in the sandy shore surf zone of Dolsando, southern Korea. Zonation pattern of three dominant gammarid amphipods was compared. The amphipods were more abundant on the bottom and at water's edge than in the surface. Average densities at both sites of Pontogeneia rostrata and Allorchestes angusta were higher during the neap tide than the spring tide, whereas that of Synchelidium lenorostralum was lower during the neap tide. P. rostrata migrated horizontally during the flooding and ebbing tides, but S. lenorostralum and A. angusta did not. Unlike other species, P. rostrata was significantly more abundant at night, suggesting its active nocturnal movement. During flooding tide, P. rostrata was not found on the shore above the mean sea level (MSL) during daytime, but found in 100 cm above MSL at night. Zonal distribution of P. rostrata which was restricted from MSL to 250 cm below MSL, however, did not vary with the day-night cycle during ebbing tide. S. lenorostralum and A. angusta were not found during flooding tide but ebbing tide. The upper distribution limit of the former was 150 cm below MSL, and the distribution of the latter ranged from MSL to 150 cm below MSL. The highest densities of P. rostrata, S. lenorostralum and A. angusta were 32, 26 and 3 ind. $m^{-2}$, respectively. We discussed the relationships between the distribution pattern of three dominant species of gammarid amphipods and their life styles in the sandy shore.
This study was conducted (1) to isolate the film-forming yeast from the commercially available soy sauce, (2) to identify the state of soy sauce fermentation by the use of yeasts, (3) to confirm characteristics of yeasts. The result were as follows. 1. These yeast strains in the soy sauce fermentation test showed full fermentation of whole sugar content, reduction of the pure extract and relative reduction in total nitrogen. Soy sauce color was somehow faded to lower the stability of soy sauce. 2. In anti-fungal activity test butylparaben at a higher level 60 ppm., sodium propionate 2,400 ppm, sodium benzoate 800 ppm., menadion 165 ppm, showed their anti-fungal effect, while alcohol did not show the effect in the 3% additive group. 3. The optimum sodium chloride concentration for these strains in the 2% G.Y.P. medium was 5% and optimum temperature was $30^{\circ}C$. The extinction temperature was $62^{\circ}C$ for strain No-1 and No-3, and was $65^{\circ}C$ for No-2 and No-4. 4. The film-forming soy sauce turned out in the gas chromatogram to possess much flavor of low boiling point as compared with the standard. These flavors were considered due to flavor spoilage of the soy sauce. 5. These isolated yeasts were identified Saccharomyces rouxii (film-forming yeast) in the Lodder's taxanomic study.
Park, Cheol-Woo;Kim, Jin-ho;Seo, Yu-Kyeong;Lee, Sae-Rom;Kang, Ju-Hee;Oh, Song-Hee;Kim, Gyu-Tae;Choi, Yong-Suk;Hwang, Eui-Hwan
Imaging Science in Dentistry
/
v.47
no.3
/
pp.165-174
/
2017
Purpose: This study was performed to investigate the influence of object shape and distance from the center of the image on the volumetric accuracy of cone-beam computed tomography (CBCT) scans, according to different parameters of tube voltage and current. Materials and Methods: Four geometric objects(cylinder, cube, pyramid, and hexagon) with predefined dimensions were fabricated. The objects consisted of Teflon-perfluoroalkoxy embedded in a hydrocolloid matrix (Dupli-Coe-Loid TM; GC America Inc., Alsip, IL, USA), encased in an acrylic resin cylinder assembly. An Alphard Vega Dental CT system (Asahi Roentgen Ind. Co., Ltd, Kyoto, Japan) was used to acquire CBCT images. OnDemand 3D (CyberMed Inc., Seoul, Korea) software was used for object segmentation and image analysis. The accuracy was expressed by the volume error (VE). The VE was calculated under 3 different exposure settings. The measured volumes of the objects were compared to the true volumes for statistical analysis. Results: The mean VE ranged from -4.47% to 2.35%. There was no significant relationship between an object's shape and the VE. A significant correlation was found between the distance of the object to the center of the image and the VE. Tube voltage affected the volume measurements and the VE, but tube current did not. Conclusion: The evaluated CBCT device provided satisfactory volume measurements. To assess volume measurements, it might be sufficient to use serial scans with a high resolution, but a low dose. This information may provide useful guidance for assessing volume measurements.
15 carbon footprint product (CFP) schemes, including Korea Carbon Footprint Label, UK Carbon Trust's Carbon Reduction Label and Japan CFP are implemented in the world. A CFP describes green house gases (GHGs) emissions emitted throughout product's life cycle and is intended to reduce GHGs emissions by labeling a CFP result on product. This study calculates Korea, UK and Japan CFP result of vacuum cleaner, detergent, packagin material in order to analyze the Korea, UK and Japan CFP standards. Our results demonstrate significant differences among then calculated results because of criteria, emission factors, etc. Therefore, there are many difficulties in providing various CFP results and the international standard and guidelines for product category are needed.
To estimate the effect of the surface state of the EVA foam on an adhesion, the surface was treated with solvents having different values of a solubility parameter and a surface tension. The morphology and the contact angle were measured by SEM and contact angle tester, respectively. The value of the critical surface tension(${\gamma}_{c,0}$) of the EVA foam calculated by Zismann plot was 27.08 dyne/cm. The surface state and the ${\gamma}_c$ of the EVA foam were changed by solvent treatment. The swelling ratio of EVA foam was influenced by solubility parameter of a solvent, on the other hand the degree of change in the surface state was dependent on a surface tension of a solvent. When a surface tension of solvent. When a surface tension of solvent is lower than ${\gamma}_{c,0}$ of the EVA foam, the wettability of a solvent was good and the surface state was greatly changed. The adhesion strength of EVA foam was greatly improved to above 600% as compared to that of the untreated one. As the ${\gamma}_c$ of EVA was closed to the surface tension of the primer, the good adhesion strength was obtained.
Atenolol is a water soluble, ${\beta}_1$ selective adrenoceptor antagonist used in the treatment of angina and hypertension. It is primarily eliminated renally with minimal hepatic metabolism. The purpose of the present study was to evaluate the bioequivalence of Samchundang Atenolol (Samchundang Pharmaceutical Co., Korea.) to Tenolmin(Hyundai Pharmaceutical Ind. Co., Korea). The atenolol release from the two atenolol tablets in vitro was tested using KP VII Apparatus II method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty four normal male volunteers, 22.83$\pm$1.99 years in age and 65.82$\pm$7.15 kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After one tablet containing 50 mg of atenolol was orally administered, blood was taken at predetermined time intervals and the concentrations of atenolol in serum were determined using HPLC method with fluorescence detector. The dissolution profiles of two atenolol tablets were very similar at all dissolution media. Besides, the pharmacokinetic parameters such as $AUC_{t}$, $C_{max}$ and $T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_{t}$ and $C_{max}$ and untransformed $T_{max}$. The results showed that the differences in $AUC_{t}$, $C_{max}$ and $T_{max}$ between two tablets based on the Tenolmin were 3.74%, 4.38% and 17.77%, respectively. There were no sequence effects between two tablets in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.98)∼log(1.l1) and log(0.95)∼log(1.l5) for $AUC_{t}$ and $C_{max}$ respectively), indicating that Samchundang Atenolol tablet is bioequivalent to Tenolmin tablet.
The growth process of the Chungkook-jang that occured by utilizing such traditional methods as Bacillus natto and Bacillus subtilis method has been examined. The results of the experiment in which the changing process of elements during the storage period had been measured are as follows: 1. During the growth period, concerning any change in pH, the storage period had been declined and after 18 days pH rose above 7.0. Salt content was between $5.28{\sim}6.40%$ and Bacillus subtilis fungus showed the highest titrable acidity. 2. Moisture content was between $50.94{\sim}56.74%$ and crude protein content range was $14.44{\sim}18.60%$ indicating irregularity in pattern resulting from the testing equipment groups, whereas crude fat and crude fiber tend to decrease in general. 3. During the storage, total sugar and ethyl alcohol content in all of groups tended to decrease and after 18 days Bacillus subtilis total sugar content was the lowest. 4. Amino nitrogen and water soluble nitrogen content increased with days, but no difference was found between groups. 5. Amylase and protease activity showed irregular pattern with time, but no significant difference between groups was found.
Tuberculosis is the leading infectious disease in the world. It is urgent to develop new vaccine and treating drugs. Besides vaccines, we want to know the effects of regular swim training on TB infection in the mouse model. This study was designed to examine the effects of regular swim training on lung and spleen TB counts and $INF-\gamma$ activity in the trained mice at different temperature. The trained mice underwent a 10-wk endurance swim training (5 times/wk) in water at $29\~33^{\circ}C$ (WWG) and $21\~23^{\circ}C$(CWG) for 60 min. And they were divided into 3 groups according to the regular swim training (CG; control, WWG; warm water group, and CWG; cold water group). Mice were challenged by aerosol infection with M. tuberculosis H37Rv using an inhalation device (Glas-Col, Terre Haute, Ind.) calibrated to deliver bacteria into lungs. Three weeks after immunization, the mice were challenged. Four weeks after challenge, the mice were sacrificed and the numbers of viable bacteria in lung and spleen were determined by plating serial dilution of whole organ homogenates on nutrient Middlebrook 7H11 agar (Difco, Detroit, MI). Colonies were counted after four weeks incubation at $37^{\circ}C$. All data were expressed as mean, standard deviation by using SPSS package program (win 10.0). The result through the statistical analysis of this data were summarized as follows; In the weight changes, there were significant differences among CG, WWG, and CWG following the swim training at different temperature, and CWG was the lowest. In the change of $INF-\gamma$ following the swim training, there were significant differences (p<.05) among CG, WWG, and CWG after stimulated with media and CFP. In MTB counts, there were significant differences (p<.05) between CG and WWG in the lung. And also there were significant differences (p<.05) among CG, WWG, and CWG. These results suggest that regular swim training suppress Th1 immune response caused by decreased $INF-\gamma$ level in the WWG, Also For the WWG, highly increased level of TB counts appear in the lung and spleen compare to CG.
Spatial patterns in the macrobenthic community structure in the inter- and subtidal zones in front of the Iwon Dike and environmental variables were examined in August 2001, In total, 156 macrobenthic species(123 intertidal species and 90 subtidal species) were recorded during this study, predominately polychaetes $(40{\%})$, bivalves $(22{\%})$, and crustaceans $(22{\%})$. Polychaetes made up less than $40{\%}$ of the intertidal communitr, but more than $50{\%}$ of the subtidal community. The mean density during this study was $1,456ind./m^{2}.$ Multivariate analysis (multidimensional scaling) revealed significant differences in community structure among four regions: near the Iwon dike (B1), the high and middle intertidal zone (B2), low intertidal zone (Al), and subtidal zone (A2). The number of species, total density, and diversity (H') varied significantly among the four regions. The distribution of macrobenthic community was affected by environmental variables, such as ${\%}$ silt/clay content, total sulfide, lose of ignition, and chemical oxygen demand. These environmental variables were negatively correlated with the dominant species (Nephtys polybranchia, Umbonium thomasi, and Scoloplos armiger) in the intertidal area, but positively correlated with the dominant species (Lumbrineris cruzensis, Notomastus latericeus, and Moerella sp.) in the subtidal area. Environmental variables $({\%}\;silt/clay content and total sulfide)$ were positively correlated with the dominant species (Heteromastus filiformis) in region Bl, but negatively correlated with the dominant species (Umbonium thomasi and Scoloplos koreanus) in region B2. Amphipods Urothoe spp. and Monoculodes koreanus were the dominant species in region Al. Umbonium thomasi, the dominant species in region B2, was not found in regions Bl or Al. We suggest that the inter-specific competition for territory and exposure to seawater may be important factors controlling the macrobenthic community structure in the inter- and subtidal zones in front of the Iwon Dike.
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