• 한국환경보건학회지
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• 제19권3호
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• pp.58-63
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• 1993

In order to elucidate the alteration of drug-metabolizing enzymes and mechanism in the animal with hepatic injury and ketosis, the regulation of P450IIE was studied in the rats with heaptic injury caused by CCl$_4$ and with ketosis caused by streptozotocin and high-fat diet. P450IIE expression in liver was examined by the combination of enzyme activities, Western immunoblot, and mRNA Northern blot analyses using specific polyclonal antibody and cDNA probe for P450IIE. Enzyme activity and amounts of immunoreactive P450IIE were rapidly decreased in a time-dependent manner after a single dose of CCl$_4$ . However, the decreases in P450IIE enzyme activity and immunoreactive protein by CCl$_4$ were not accompanied by a decline in its mRNA level. The data thus suggested a post-translational reduction of P450IIE by CCl$_4$. The enzyme activities (aniline hydroxylase) in hepatic microsomes were elevated about 2-3-fold by streptozotocin and feeding with a high fat diet. This increases in enzyme activities were also accompanied by 3-fold increases in immunoreactive P450IIE protein and its mRNA. Our data thus indicated that P450IIE induction during the ketosis appears to be due to pretranslational activation.

• Carbon letters
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• 제1권1호
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• pp.36-40
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• 2000

The initial irreversible capacity (IIC) of a hard carbon during the charge/discharge reaction is strongly affected by both the initial irreversible capacity on the carbon surface $(IIC_S)$ and the initial irreversible lithium insertion into carbon $(IIC_B)$. The initial coulombic efficiency of the insertion and the desertion of lithium (IIE) can be used as a performance to classify $IIC_B$ of the carbon. The $IIC_B$ was proportional to the specific discharge capacity with a slope, $IIE^{-1}$ - 1. The IIE of hard carbon had four regions. $IIE_A$ for the region of 0~95 mAh/g of $Q_{D1}$ was 60.2%. $IIE_B$ and $IIE_C$ for the regions of 95~172 mAh/g and 172~308 mAh/g had 84.9% and 91.5%, respectively. $IIE_D$ was appeared above 308 mAh/g. But, the $IIE_D$ was reduced to 82.1% compared with $IIE_C$. These IIE might be corresponding to lithium desertion from carbon at the region of 0~172 mAh/g range, lithium desertion from the micropore of carbon at the region of 172~308 mAh/g range, and to the lithium stripping of the plated lithium for the region above 308 mAh/g, respectively.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1992년도 제1회 신약개발 연구발표회 초록집
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• pp.54-54
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• 1992

약물, hormone, 독성물질등의 대사능과 발암 가능성등이 간장 장해시 및 ketosis시에 달라지는 원인과 기전, 독성물질 대사효소의 변동과 그 작용기전을 규명하고자, 대표적인 간장장해 물질인 carbon tetrachloride를 rat에 투여하여 간장 장해를 일으키고, 당뇨병, starvation, high-fat diet처리하여 ketosls상태를 만든 후에, specific cytochrome P45O polyclonal antibodies와 cDNA probes를 사용하여, enzyme activitieg, Western immunoblot analysis와 mRNA Northern blot analysis 등을 실험하여, 간장 장해와 ketosis시 cytochrome P45O의 변동과 그 작용기전, regulation을 규명하고자 하였다. 실험 결과, $CCl_4$투여후 P450IIE enzyme (aniline hydroxylase) 활성이 시간 의존적으로 급격히 떨어졌고, P450IIE protein양이 똑같은 방식으로 감소되었으나 mRNA level은 변화가 없었다. $CCl_4$에 의해서 P450IIE는 protein의 특이적인 파괴에 의한 post-translational reduction됨을 알 수 있었다. 반면에 당뇨병, starvation, high-fat diet등 ketosis시에는 P450IIE 효소활성이 2-3배 증가되었고, P450IIE protein양도 같은 수준으로 증가되었으며, mRNA도 증가 되었다. Ketosis시에는 P450IIE가 pretranslational activation됨을 알 수 있었다.

• 한국작물학회지
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• 제50권spc1호
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• pp.8-11
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• 2005

자광찰벼, 화선찰벼, 및 일품벼 에탄올 분획의 H4IIE 세포에 대찬 산화적 스트레스 보호효과를 검정하기 위해서 수행하였다. 과산화수소에 의하여 생성된 자유라디칼은 H4IIE 세포에 산화적 손상을 초래하며 이러한 손상에 기인된 세포사멸에 대하여 화선찰벼와 일품벼 추출물은 방어효과가 없었으나, 적색을 띤 자광찰벼 추출물의 에탄올 분획은 과산화수소에 의하여 유도된 산화적 스트레스에 가장 현저한 효과를 나타내었다. 금후, 보다 광범위하고 집중적인 연구를 수행한다면 쌀 함유 유효성분들의 생리활성에 관한 보다 명확한 효과가 구명될 수 있을 것으로 사료된다.

• Carbon letters
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• 제1권3_4호
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• pp.148-153
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• 2001

The initial irreversible capacity, $Q_i$, is one of the parameters to express the material balancing of the cathode to anode. We introduced new terms, which are the initial intercalation Ah efficiency (IIE) and the initial irreversible specific capacity at the surface ($Q_{is}$), to express precisely the irreversibility of an electrode/electrolyte system. Two terms depended on kinds of active-materials and compositions of the electrode, but did not change with charging state. MPCF had the highest value of IIE and the lowest value of $Q_{is}$ in 1M $LiPE_6$/EC + DEC (1 : 1 volume ratio) electrolyte. IIE value of $LiCoO_2$ electrode was 97-98%, although the preparation condition of the material and the electrolyte were different. $Q_{is}$ value of $LiCoO_2$ was 0~1 mAh/g. MPCF-$LiCoO_2$ cell system had the lowest of the latent capacity. $Q_{is}$ value increased slightly by adding conductive material. IIE and $Q_{is}$ value varied with the electrolyte. By introducing PC to EC+DEC mixed solvent, IIE values were retained, but $Q_{is}$ increased. In case of addition of MP, IIE value increased and $Q_{is}$ value also increased a little.

• KIEE International Transactions on Electrophysics and Applications
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• 제3C권5호
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• pp.189-193
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• 2003

Initial irreversible capacity (IIC) can be defined by means of the initial intercalation Ah efficiency (IIE) and the initial irreversible specific capacity at the surface (IICs) with the linear-fit range of the intercalation so as to precisely express the irreversibility of an electrode-electrolyte system. Their relationship was IIC = Qc - Q$_{D}$ = (IIE$^{-1}$ - 1) Q$_{D}$ + IICs in the linear-fit range of IIE. Here, Qc and Qd signify charge and discharge capacity, respectively, based on a complete lithium ion battery cell. Charge indicates lithium insertion to carbon anode. Two terms of IIE and IICs depended on the types of active materials and compositions of the electrode and electrolyte but did not change with charging state. In an ideal electrode-electrolyte system, IIE and IICs would be 100%, 0 mAh/g for the electrode and mAh for the cell, respectively. These properties can be easily obtained by the Gradual Increasing of State of Charge (GISOC).OC).

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제53권2호
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• pp.53-61
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• 2004

Characteristics of half cells of graphite/lithium and LiCoO$_2$/lithium, and full cells of graphite/LiCoO$_2$/ were analyzed by the use of GISOC(the gradual increasing of the state of charge). GISOC analyses generated IIE(the initial intercalation efficiency), which represents lithium intercalation property of the electrode material, and IIC$_{s}$(the initial irreversible capacity by the surface), which represents irreversible reaction between the electrode surface and electrolyte. Linear-fit range of graphite and LiCo/O$_2$electrodes were respectively 370 and 150 mAh/g based on material weight. IIE of graphite and LiCo/O$_2$electrodes were respectively 93∼94 % and 94∼95 %, and IICs of graphite and LiCo/O$_2$electrodes were 15∼17 mAH/g and 0.3∼1.7 mAh/g, respectively. IIE of graphite/LiCo/O$_2$full cell for GX25 and DJG311 as graphite showed 89∼90 %, which IIE value was lower than IIE of half cell of the cathode and the anode. Parameters of IIE and IIC$_{s}$ can also be used to represent not only half cell but also full cell. The characteristics of the full cell can be simulated through the correlative interpretation of potential profile, IIE, and IIC$_{s}$ of half cells.cells.

• Journal of Medicine and Life Science
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• 제17권2호
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• pp.41-46
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• 2020

Metformin, a predominantly prescribed anti-diabetic drug for decades, has gained new insights for its anti-tumor activity in a variety of cancer cells. Our previous studies also showed the obvious pro-apoptotic activity of metformin and the underlying action mechanisms in hepatocellular carcinoma cells. Together with apoptosis, autophagy is a crucial intracellular process to determine the survival or death of cells under some stressful environments. The present study aimed to determine the role of autophagy in metformin-induced death of H4IIE hepatocellular carcinoma cells. Metformin blocked the formation of autophagosome and the expression of LC3A, generally described as a biomarker of autophagy. Inhibition of AMPK reversed the metformin-induced blockade of autophagy. Antioxidant (NAC) suppressed the metformin-induced cell death but not affected LC3A. The inhibition of protein kinase C totally restored the metformin-suppressed expression of LC3A. In summary, our present study suggests that autophagy is an anti-apoptotic player in metformin-induced apoptosis in H4IIE cells.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 추계 학술대회 학술발표 논문집
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• pp.99-102
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• 2003

A new interacting multiple model (IMM) method using genetic algorithm (GA)-based intelligent input estimation(IIE) is proposed to track a maneuvering target. In the proposed method, the acceleration level for each sub-model is determined by IIE-the estimation of the unknown acceleration input by a fuzzy system using the relation between maneuvering filter residual and non-maneuvering one. The GA is utilized to optimize a fuzzy system fur a sub-model within a fixed range of acceleration input. Then, multiple models are composed of these fuzzy systems, which are optimized for different ranges of acceleration input. In computer simulation for an incoming ballistic missile, the tracking performance of the proposed method is compared with those of the input estimation(IE) technique and the adaptive interacting multiple model (AIMM) method.

• International Journal of Contents
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• 제5권4호
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• pp.55-61
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• 2009

In this paper, we propose a hierarchical method for segmenting a given 3D mesh, which hierarchically clusters sharp vertices of the mesh using the metric of geodesic distance among them. Sharp vertices are extracted from the mesh by analyzing convexity that reflects global geometry. As well as speeding up the computing time, the sharp vertices of this kind avoid the problem of local optima that may occur when feature points are extracted by analyzing the convexity that reflects local geometry. For obtaining more effective results, the sharp vertices are categorized according to the priority from the viewpoint of cognitive science, and the reasonable number of clusters is automatically determined by analyzing the geometric features of the mesh.