Antioxidant Activity of Jakwangchalbyeo Extracts in H4IIE Cells

자광찰벼 추출물의 H4IIE 세포에서의 항산화 효과

  • Published : 2005.11.01

Abstract

Experiments were performed to investigate the effects of ethanol fraction of three different rice (Oryzasativa L. var. japonica) grain (Jakwangchalbyeo, red-pericarp glutinous rice; Hwasunchalbyeo, white-pericarp glutinous rice; Ilpumbyeo, white-pericarp non-glutinous rice) extracts on the protection of oxidative stress. Antioxidant activities of ethanol fraction of rice grain extracts were made with MTT cell viability assay in H4IIE cell that is challenged with hydrogen peroxide. Hwasunchalbyeo extract and Ilpumbyeo extract did not show any significant protective effects on the $H_2O_2-induced$ oxidative stress in B4IIE cells, and Jakwangchalbyeo extract improved the cell viability up to $82\%\;and\;74\%$ at concentration of $100{\mu}g/mL$ for 5 h and 24 h treatment, respectively. In conclusion, red-pericarp Jakwangchalbyeo extract as compared with other rice extracts exerted significant inhibitory effects on the hydyogen peroxideinduced oxidative stress in the H4IIE cells.

자광찰벼, 화선찰벼, 및 일품벼 에탄올 분획의 H4IIE 세포에 대찬 산화적 스트레스 보호효과를 검정하기 위해서 수행하였다. 과산화수소에 의하여 생성된 자유라디칼은 H4IIE 세포에 산화적 손상을 초래하며 이러한 손상에 기인된 세포사멸에 대하여 화선찰벼와 일품벼 추출물은 방어효과가 없었으나, 적색을 띤 자광찰벼 추출물의 에탄올 분획은 과산화수소에 의하여 유도된 산화적 스트레스에 가장 현저한 효과를 나타내었다. 금후, 보다 광범위하고 집중적인 연구를 수행한다면 쌀 함유 유효성분들의 생리활성에 관한 보다 명확한 효과가 구명될 수 있을 것으로 사료된다.

Keywords

References

  1. Cho, M.H, H.H. Yoon, and T.R. Hahn. 1996. Thermal stability of themajor color compound, cyanidin-3-g1ucoside, from a korean pigmentrice variety in aqueous solution. Agricultural Chemistry andBiotechnology. 39 : 245-248.
  2. Choi, H.C. and S.K. Oh. 1996. Diversity and function of pigment incolored rice. Korean Journal ofCrop Science. 41 : 1-9.
  3. Choi, S.W, S.H. Nam, and l-l.C. Choi. 1996. Antioxidative activity ofethanolic extracts of rice brans. Food Science and Biotechnology.5: 305-309.
  4. Choi, S.W, WW Kang, T. Osawa, and S. Kawakishi. 1994. Antioxidativeactivity of crysanthemin in black rice hulls. Food Scienceand Biotechnology. 3 : 233-237.
  5. Chung, LM., KH. Kim, lK. Ahn, S.K. Lee, and J.O. Lee. 2001. Antioxidativeactivity of rice grain using FI-CL and ESR methods.Korean Journal ofCrop Science. 46 : 411-415.
  6. Chung, I.M., KH. Kim, lK. Ahn, and 10. Lee. 2000 a. Varietal variationin antioxidative activity of rice grain by DPPH and TBAmethods. Korean Journal ofCrop Science. 45 : 261-266.
  7. Chung, LM., KH. Kim, rx. Ahn, and J.O. Lee. 2000 b. Comparisonof superoxide dismutase and peroxidase activities in rice varieties.Korean Journal ofCrop Science. 45 : 277-281.
  8. Choi, Y.I., lS. Choi, S.H. Lee, Y.I. Lee, J.S. Kang, and Y.H. Kang.2002. Inhibitory effects ofEpigallocatechin Gallate on apoptosis inhuman vascular endothelial cells. Journal of Food Science andNutrition. 31 : 672-678.
  9. Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cellgrowth and survival: modifications to the tetrazolium dye proceduregiving improved sensitivity and reliability. Journal of Immunological Methods. 89 : 271-277.
  10. Kang, K.W, R.F. Novak, C.H. Lee, and S.G Kim. 2002. Induction ofmicrosomal epoxide hydrolase by sulfur amino acid deprivationvia the pathway of C-Jun N-terminal kinase and its extracellularexposure during cell death. Free Radical Biology & Medicine. 32 :1017-1032.
  11. Kwak, T.S., HJ. Park, WT. Jung, and lW Choi. 1999. Antixoidativeand hepatoprotective activity of coloured-, scented and Koreannative rice varieties based on different layers. Journal ofFood Scienceand Nutrition. 28: 191-198.
  12. Lee, Y.S. 2001. Effect of isoflavones on proliferation and oxidativestress of MC3T3-El osteoblast-like cells. Korea Soybean Digest.18: 35-42.
  13. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth andsurvival: application to proliferation and cytotoxicity assays. JournalofImmunological Methods. 65: 55-63.
  14. Nam, S.H., S.M. Chang, and M.Y. Kang. 2003. Varietal difference inantioxidative activity of ethanolic extracts from colored rice bran.Agricultural Chemistry and Biotechnology. 46: 16-22.
  15. Osawa, T.,N. Ramarathnam, K. Shunro, N. Mitsuo, and T. Torn. 1985.Antioxidative defense system in rice hull against damage causedby oxyen radicals. Agricultural and Biological Chemistry. 49 :3085-3087.
  16. Park, DJ., GY. Na, SJ. Lee, D.W Kim, and S.L. Chung. 2003.Increased sensitivity ofkeratinocytes to oxidative stress in vitiligo.Korean Journal ofDermatology. 41 : 592-596.
  17. Ramarathnam, N., T. Osawa, M. Namiki, and T. Tashiro. 1986. Studieson the relationship between antioxidative activity of rice hull andgermination ability of rice seed. Journal ofthe Science ofFood andAgriculture. 37 : 719-726.
  18. Ramarathnam, N., T. Osawa, M. Namiki, and T. Tashiro. 1989. Chemicalstudies on novel rice hull antioxidants. 2. Identification of isovitexin,a C-glycosyl flavnonoid. Journal of Agricultural and FoodChemistry. 37: 316-319.
  19. Tsuda, T., M. Watanabe, K. Ohshima, S. Norinobu, S.W Choi, S.Kawakishi, and T. Osawa. 1994. Antioxidative activity of the antocyaninpigments cyanidin 3-0-f3-D-glucoside and cyanidin. JournalofAgricultural and Food Chemistry. 42 : 2407-2410.
  20. Wu, K, W Zhang, P.B. Addis, R.I. Epley, A.M. Salih, and 1 Lehrfeld.1994. Antioxidant properties of wild rice. Journal of Agriculturaland Food Chemistry. 42: 34-37.