• 대한간호학회지
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• 제34권1호
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• pp.150-159
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• 2004

Purpose: The purpose of this study was to determine the effect of DHEA on hindlimb muscles(soleus, plantaris and gastrocnemius) in a focal brain ischemia model rat. Method: Twenty-seven male Sprague-Dawley rats were randomly divided into three groups: CINS(cerebral ischemia + normal saline), CIDH(cerebral ischemia + DHEA), or SHNS(sham + normal saline). Both the CINS and CIDH groups underwent a transient right middle cerebral artery occlusion operation. In the SHNS group, a sham operation was done. 0.34mmol/kg DHEA was administered daily by an intraperitoneal injection for 7days. Results: The muscle weight, muscle fiber cross-sectional area of the Type I muscle fiber of soleus and Type II muscle fiber of plantaris and gastrocnemius, myofibrillar protein content of gastrocnemius, and muscle strength in the CINS group decreased compared with the SHNS group. The muscle weight, muscle fiber cross-sectional area of the Type II muscle fiber of plantaris and gastrocnemius, myofibrillar protein content of soleus, and muscle strength in the CIDH group increased compared with the CINS group. Conclusion: It was identified that muscle atrophy could be induced during 7 days after a cerebral infarction, and DHEA administration during the early stages of a cerebral infarction might attenuate muscle atrophy.

• 대한물리의학회지
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• 제18권1호
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• pp.1-8
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• 2023

PURPOSE: This study analyzed how proprioceptive neuromuscular facilitation (PNF) combined with auricular acupuncture affected the activation of the leg muscles of stroke patients and measured the effects of this combination to provide clinical data. METHODS: The subjects were divided randomly into experimental group I, which received PNF combined with auricular acupuncture, and experimental group II, which received PNF alone. Each group had ten members. A 30-minute intervention was performed four days a week for six weeks. Before the six weeks of intervention, pre-tests were conducted to measure muscle activation in the legs. After six weeks, post-tests were also conducted to measure muscle activation in the legs. RESULTS: Experimental group I showed a statistically significant difference in muscle rectus femoris, muscle biceps femoris, muscle tibialis anterior, and muscle soleus. Experimental group II also showed a statistically significant difference in the muscle rectus femoris, muscle biceps femoris, muscle tibialis anterior, and muscle soleus (p < .05). In a between-group comparison of the changes, a statistically significant difference was observed between the two groups in terms of muscle rectus femoris, muscle biceps femoris, muscle tibialis anterior, and muscle soleus (p < .05). CONCLUSION: Intervention in experimental group I increased the activation of the leg muscles more effectively because auricular acupuncture was applied to various spots on the ear corresponding to the spleen, liver, kidney, pelvis, knee, ankle, and toe. Auricular acupuncture is expected to be used more widely in the future because it is a safe way of stimulating muscle activation.

• The Journal of Korean Physical Therapy
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• 제20권1호
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• pp.57-65
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• 2008

Purpose: Heat shock proteins (HSPs) are a group of proteins that are activated when cells are exposed to a variety of environmental stresses, such as infection, inflammation, exposure to toxins, starvation, hypoxia, brain injury, or water deprivation. The activation of HSPs by environmental stress plays a key role in signal transduction, including cytoprotection, molecular chaperone, anti-apoptotic effect, and anti-aging effects. However, the precise mechanism for the action of small HSPs, such as HSP27 and mitogen-activated protein kinases (MAPKs: extracellular-regulated protein kinase 1/2 (ERK1/2), p38MAPK, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), is not completely understood, particularly in application of cell stimulators including platelet-derived growth factor (PDGF), angiotensin II (AngII), tumor necrosis factor $\alpha$ (TNF$\alpha$), and $H_2O_2$. This study examined the relationship between stimulators-induced enzymatic activity of HSP27 and MAPKs from rat smooth and skeletal muscles. Methods: 2-dimensional electrophoresis (2DE) and matrix assisted laser desorption ionizationtime-of-flight/time-of-flight (MALDI-TOF/TOF) analysis were used to identify HSP27 from the intact vascular smooth and skeletal muscles. Three isoforms of HSP27 were detected on silver-stained gels of the whole protein extracts from the rat aortic smooth and skeletal muscle strips. Results: The expression of PDGF, AngII, TNF$\alpha$, and $H_2O_2$-induced activation of HSP27, p38MAPK, ERK1/2, and SAPK/JNK was higher in the smooth muscle cells than the control. SB203580 (30${\mu}$M), a p38MAPK inhibitor, increased the level of HSP27 phosphorylation induced by stimulators in smooth muscle cells. Furthermore, the age-related and starvation-induced activation of HSP27 was higher in skeletal muscle cells (L6 myoblast cell lines) and muscle strips than the control. Conclusion: These results suggest, in part, that the activity of HSP27 and MAPKs affect stressors, such as PDGF, AngII, TNF$\alpha$, $H_2O_2$, and starvation in rat smooth and skeletal muscles. However, more systemic research will be needed into physical therapy, including thermotherapy, electrotherapy, radiotherapy and others.

• 한국운동역학회지
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• 제20권1호
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• pp.75-81
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• 2010

Knee osteoarthritis is one of the most prevalent arthritis that weakens the muscles. This study focused on evaluating muscular functionality of knee osteoarthritis subjects. Muscular strengths of muscles around knee and hip joints of middle-aged female subjects aged over 40 suffering from knee osteoarthritis were evaluated by isokinetic dynanometer. Also, relation between the observed muscle imbalance in knee and hip joints and visual analogue scale was investigated. Subjects performed 8-weeks exercise on weakened muscles - particularly on knee extensors and hip abductors - and had their isokinetic muscular functionalities analyzed again. After the 8-weeks exercise, subjects' thigh circumferences were increased, accompanied with muscular strength improvements and decrease in visual analogue scale. Hence we emphasize the importance of exercise for muscular strength enhancement of knee extensors and hip abductors, in rehabilitation programs for knee osteoarthritis.

• The Journal of Korean Physical Therapy
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• 제11권1호
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• pp.111-119
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• 1999

The object of study who healthufl thirty persons have been enforced Isokinetic exercise of non-dominant muscular strength. The next same that each dominant muscular strength and non-dominant strength, peak torque and total work have been comparative analysis 1, Shoulder muscles comparion increase peak torque at low speed from pretraining Isokinetic exercise of non-dominant strength side to ten week of post-training.. Flexor and extensor come out high and statistically significant 6, 8, 10 week than pretraining. Adductor and abductor come out high and statistically significant 4, 6, 8. 10 week then pre-training, Internal rotator and external rotator come out statistically significant 2, 4, 6, 8, 10 week then pre-ttraining. 2. Shoulder muscles comparion increase peak torque at high speed from pre-training Isokinetic execise of non-dominant strenght side to ten week of post-training. Flexor and extensor come out high and statistically significant 4, 6, 8, 10 week then pie-training. Adductor and abductor come out high and statistically significant 2, 4, 6, 8, 10 week than pre-training. Futernal rotator and external rotator come out statistically significant 4. 6, 8, 10 week than pre-training.

• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1496-1502
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• 2006

Skeletal muscle contains slow and fast twitch fibers. These skeletal muscle fibers express type I and type II myosin, respectively, and these myosin isoenzymes have different ATPase activity. The aim of this study was to investigate protein profiles of bovine skeletal muscles by proteomic analysis. Fifty seven spots of distinct proteins were excised and characterized. The expression of sixteen spots was differed in longissimus dorsi muscle with a minimal 2-fold change compared to biceps femoris muscle. The majority of differentially expressed proteins belonged to metabolic regulation-related proteins such as glyceraldehyde 3-phosphate dehydrogenase, triosephosphate isomerase and carbonic anhydrase 3. The real time-PCR assay confirmed an increase or induction of specific genes: RGS12TS isoform, GAPDH, triosephosphate isomerase and carbonic anhydrase. These results suggest that the expression of metabolic proteins is under a specific control system in different bovine skeletal muscle. These observations could have significant implications for understanding the physiological regulation of bovine skeletal muscles.

• Journal of Korean Biological Nursing Science
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• 제4권2호
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• pp.19-40
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• 2002

The purpose of this study was to determine the effect of DHEA on Type I(soleus) and II muscles(plantaris, gastrocnemius) in a focal brain ischemia model rat. Thirty-seven male Sprague-Dawley rats with $200{\sim}250g$ body weights were randomly divided into four groups : CINS(cerebral ischemia + normal saline), CIDH(cerebral ischemia + DHEA), SHNS(sham + normal saline), SHDH (sham + DHEA). Both the CINS and CIDH groups were undergone a transient right middle cerebral artery occlusion operation. In the SHNS and SHDH groups, a sham operation was done. DHEA was administered daily at a dose of 0.34mmol/kg, and normal saline was administered daily at the same dose by intraperitoneal injection for 7days after operation. Cerebral infarction in the CINS and CIDH groups was identified by staining with 2% triphenyltetrazolium chloride solution for 60 minutes. The data were analyzed by Kruskal-Wallis test and Mann-Whitney U test using the SPSSWIN 9.0 program. The results were summarized as follows: 1) The muscle weights of soleus(Type I), plantaris and gastrocnemius(Type II) in CINS group were significantly less than those of the SHNS group(p<.01). The muscle fiber cross-sectional area of the CINS group was significantly less than that of the SHNS group in Type I muscle fiber of the soleus and Type II muscle fiber of the plantaris and gastrocnemius(p<.05). The myofibrillar protein content of the CINS group was significantly less than that of the SHNS group in the left gastrocnemius and right soleus(p<.05). 2) The muscle weights of the soleus, plantaris and gastrocnemius except the unaffected side of the plantaris in the CIDH significantly increased compared to those of the CINS group(p<.05). The muscle fiber cross-sectional area of the CIDH group significantly increased compared to that of the CINS group in Type II muscle fiber of the plantaris and gastrocnemius(p<.05). The myofibrillar protein content of the CIDH group significantly increased compared to that of the CINS group in the left soleus(p<.05). 3) On the post-op 8 day, the body weight of the CINS group was significantly less than that of the CIDH, SHNS and SHDH groups(p<.01). Total diet intake of the CINS and CIDH groups was significantly less than that of the SHNS and SHDH groups(p<.01). Based on these results, it was identified that muscle atrophy could be induced during the 7 days after cerebral infarction, and DHEA administration during the early stage of cerebral infarction might attenuate muscle atrophy.

• 대한통합의학회지
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• 제1권4호
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• pp.85-92
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• 2013

PURPOSE : The purpose of this study was to determine the effects of the length of the pectoralis minor on muscle activity of trapezius and pectoralis major in subjects in subjects with shortened pectoralis minor muscle. METHOD : The subjects was participated in 36 with shortened pectoralis minor muscle. All subjects was examined the length test of pectoralis minor muscle. we divided by 3 groups. group I(n=12) was for 4~5cm of length of pectoralis minor muscle, group II(n=12) was for 5~6cm, group III(n=12) was for above 6cm. The EMG activity of upper trapezius, middle trapezius, lower trapezius and pectoralis major muscle activity was measured by surface EMG while elevationg the right arm in sitting postion with head to the neutral, shoulder elevation $135^{\circ}$ with scaption. Data were analyed using one-way ANOVA with a Tukey post hoc test. RESULT : The EMG activity differed significantly among the three groups(p<.05). The group III had significantly greater EMG activity of upper trapezius and pectoralis major muscles than group I and II(p<.05). Also, The group III had significantly smaller EMG activity of lower trapezius muscle than group I and II(p<.05). But, these was no significant difference in the EMG activity of the middle trapezius muscle among the groups (p.05). CONCLUSION : Therefore, the result of this study should be suggested that the shortened pectoralis minor muscle was affected the EMG activity of the upper trapezius, lower trapezius and pectoralis major. Ultimately the length of the pectoralis minor muscle leads to the muscle imbalance in shoulder girdle.

• The Journal of Korean Physical Therapy
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• 제28권1호
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• pp.52-58
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• 2016

Purpose: This study intended to mediate shoulder exercise using grasping and investigate examine how its effects on affects activities of the shoulder surrounding muscles of the shoulder and thickness of shoulder muscle to suggest effective exercise for patients with rotator cuff repair to return to daily life. Methods: This study has been done targeteding male patients more than 6 weeks after having En-masse Suture Bridge Technique as rotator cuff repair of the right shoulder, aged 40 to 55 in a medical institution located in Jeollanam-do. Experimental group I (n=15) was selected for a conducting common exercise therapy program and Experimental group II (n=15) was selected for an conducting exercise therapy program using grasping. %RVC was measured by surface electromyography and muscle thickness was measured by ultrasound before mediation to for analysisze of before and after results within group and between groups. Results: In comparison of change of %RVC, Tthere were meaningful differences in only posterior deltoid and infraspinatus in comparing a change of %RVC within experimental group I (p<0.001),. and Tthere were meaningful differences in both supraspinatus and infraspinatus in muscle thickness within experimental group I (p<0.001). In comparison of change of %RVC, Tthere were meaningful differences in anterior deltoid, posterior deltoid and infraspinatus in comparing a change of %RVC within experimental group II (p<0.05)(p<0.01) (p<0.001), and there were meaningful differences in both supraspinatus and infraspinatus in muscle thickness within experimental group II (p<0.001). In comparison of change of %RVC between groups, Tthere was a meaningful difference in only posterior deltoid in comparing a change of %RVC between groups (p<0.001). Conclusion: We found that exercise therapy using grasping has a positive effect on shoulder stability muscles such as supraspinatus muscle and infraspinatus muscle and activity of deltoid muscle.

• 현장농수산연구지
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• 제11권1호
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• pp.53-61
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• 2009

본 연구는 개발된 밀리타리스 동충하초 균사체를 비육후기 한우에 사료경제적인 한계의 최고점에서 일정기간 급여하여 균사체내에 존재하는 cordycepin이 한우 조직내에 존재하는 지와 혈중 항산화활성의 증가를 확인하기 위하여 수행하였다. 본 연구는 비육후기 거세한우 4두를 공시하여 대조구와 처리구 각각 2두씩 배치하고, 처리구의 균사체 첨가수준은 사료섭취량의 1%로 정하여 80일간 사양하였다. 사양후 도축하여 근육(후지)을 취하여 이들내 cordycepin을 TLC상에서 전개하고, 혈액을 채취하여 혈장내 항산화효소인 GSH-Px활성을 조사하였다. 본 연구에서 얻어진 결과를 살펴보면, 종료체중, 건물섭취량, 중체량은 대조구 및 밀리타리스 동충하초 균사체급여군간에 유의한 차이가 나타나지 않았다. 혈장내 GSH-Px활성은 처리구가 15.70 unit로서 대조구의 9.23 unit보다 높은 항산화도를 나타내었으나, 통계적으로 유의한 차이는 나타나지 않았다. 근육내 cordycepin의 전이는 곡립 밀리타리스 동충하초 균사체 급여군에서 TLC상 band가 확인되었다. 이상의 결과로부터 밀라티리스 동충하초 균사체를 비육후기 거세우에 급여하였을 때 비록 유의성은 나타나지 않았지만, 항산화 기능이 어느 정도 있는 것으로 확인되었고, 처리군의 근육에서 cordycepin이 확인되었다. 따라서, 곡립 밀리타리스 동충하초 균사체를 한우에 급여하면 조직 내의 cordycepin의 전이가 가능한 것으로 사료된다.