• 약학회지
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• 제59권4호
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• pp.170-176
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• 2015

In vaccine development, the major points may be induction of effective and increased levels of antibody production. This is especially the case when the antigenic sources are carbohydrates. For many years, thus, we have researched various types of formulations such as liposomal and conjugate vaccines. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested if platycodin D (PLD) from Platycodon Radix have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that in the murine model of antibody production, CACW combined with PLD [CACW/PLD/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CACW/PLD/IFA], the antibody production was 1.4 times as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity maintaining the initial titers of antibody as compared to the CFA formula. Cytokine profiling with the antisera displayed that the PLD produced both Th1 and Th2 immunoresponses, but Th1 dominant was much greater (P<0.05). Furthermore, [CACW/PLD/IFA] formula enhanced resistance of mice against disseminated candidiasis, whereas the CFA had no such effect. In conclusion, PLD has an immunologic activity, which is protective against the disease. Thus, PLD can be a goof candidate for a new immunoadjuvant in development of the fungal vaccine.

• 대한바이러스학회지
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• 제28권2호
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• pp.193-201
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• 1998

In Sprague Dawley (SD) rats, antibodies against strains of Orientia tsutsugamushi, Kato, Karp and Gilliam, were produced in order to investigate their longevity and cross-reactivities to their corresponding homologous and heterologous antigens. By immunofluorescence assay (IFA) of IgG and IgM, it was shown that the immunity to the homologous strains persisted at a higher level (longevity of at least 34 weeks with higher IFA titers). On the other hand, the immunity to the heterologous strains persisted at a lower level (longevity of 10 to 34 weeks with lower IFA titers). Since infection with one strain of O. tsutsugamushi does not preclude reinfection with other strains, understanding of the antigenic diversity of O. tsutsugamushi and duration of the immunity to both homologous and heterologous strain is very important in diagnosis of scrub typhus.

• Membrane and Water Treatment
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• 제9권3호
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• pp.155-162
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• 2018

Process modeling with activated sludge models (ASMs) is useful for the design and operational improvement of biological nutrient removal (BNR) processes. Effective utilization of ASMs requires the influent fraction analysis (IFA) of the wastewater treatment plant (WWTP). However, this is difficult due to the time and cost involved in the design and operation steps, thereby declining the simulation reliability. Harmony Search (HS) algorithm was utilized herein to determine the relationships between composite variables and state variables of the model IWA ASM1. Influent fraction analysis was used in estimating fractions of the state variables of the WWTP influent and its application to 9 wastewater treatment processes in South Korea. The results of influent $S_s$ and $Xs+X_{BH}$, which are the most sensitive variables for design of activated sludge process, are estimated within the error ranges of 8.9-14.2% and 3.8-6.4%, respectively. Utilizing the chemical oxygen demand (COD) fraction analysis for influent wastewater, it was possible to predict the concentrations of treated organic matter and nitrogen in 9 full scale BNR processes with high accuracy. In addition, the results of daily influent fraction analysis (D-IFA) method were superior to those of the constant influent fraction analysis (C-IFA) method.

• 한국동물위생학회지
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• 제35권1호
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• pp.19-24
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• 2012

This study was performed to evaluate the prevalence rate of Toxoplasma gondii on 217 stray cats in Daejeon. The positive infection rate of T. gondii was 15.7% in enzyme-linked immunosorbent assay (ELISA), 12.4% in latex agglutination test (LAT), 14.7% in indirect immunofluorescent antibody test (IFA) and 0.5% in polymerase chain reaction (PCR) respectively. In districts, Yuseong-gu was shown the highest seropositive rate of T. gondii as 31.8% in ELISA, 22.7% in LAT and 31.8% in IFA. In gender, the seropositive rate of female cats was slightly higher than that of male cats as 17.2% in ELISA, 15.2% in LAT, 15.2% in IFA and 1.0% in PCR. Cats captured in National science museum, detached house and apartment was shown relatively high prevalence rate of T. gondii.

• 한국동물위생학회지
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• 제32권1호
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• pp.19-25
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• 2009

Porcine reproductive and respiratory syndrome (PRRS) virus is the etiological agent of diseases characterized by reproductive losses in sows and respiratory disorders in piglets. The PRRS virus is a small enveloped virus containing a positive-sense, single-stranded RNA genome. In the present study, ORF6 gene of Korean PRRS virus isolate, CNV, was cloned and expressed in baculovirus expression system. The ORF6 gene and expressed protein in the recombinant virus were confirmed by PCR/indirect fluorescence antibody (IFA) test and Western blotting, respectively. The recombinant protein with a molecular weight of approximately 24KDa was confirmed by Western blotting using His6 and PRRS virus-specific antiserum. Expressed ORF6 protein was applied for IFA to detect antibody against PRRS virus using field porcine sera. However, the sensitivity and specificity of developed IFA using expressed ORF6 protein were considerably low compared to those of commercial ELISA kit. This results suggest that IFA using expressed ORF6 protein could not be used as a diagnostic test for PRRS virus infection without further improvements.

• 한국전자파학회논문지
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• 제26권9호
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• pp.769-776
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• 2015

본 논문에서는 메탈 커버를 방사소자의 일환으로 적용한 광대역 MIMO 안테나를 설계하고, 이를 PCB 기판에 구현한다. 제안 안테나는 모노폴 안테나와 모노폴로부터 커플링 급전을 받는 IFA 및 메탈 커버 방사체로 구성된다. 따라서 모노폴 안테나와 IFA 및 메탈 커버가 동시에 동작하는 하이브리드 안테나가 된다. 제안 안테나는 LTE class 13/ LTE class 14/ CDMA/ GSM900/ DCS/ PCS/ W-CDMA/ LTE class 40/WiFi 대역에서 VSWR 3:1 이하의 조건을 만족하였다. 상하 대각선 급전 MIMO 안테나의 최대 포락선 상관계수는 0.186이다. 방사 측정 결과, 전 대역에서 -5.14~-1.28 dBi의 평균 이득과 30.87~74.48 %의 효율을 얻었다.

• 한국전자파학회논문지
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• 제22권7호
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• pp.737-743
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• 2011

본 논문에서는 커플링 급전 구조를 사용하여 모든 이동 통신 대역을 만족하는 단말기용 모노폴+IFA(Inverted F Antenna) 하이브리드 안테나를 설계하고 이를 제작하였다. 1개의 급전 구조를 가지면서도 커플링을 이용하여 모노폴과 IFA가 동시에 동작하도록 함으로써 넓은 대역 특성을 갖도록 하였다. 안테나는 저가격 실현을 위해 carrier 부품이 없는 PCB embedded 형태로 설계하였다. 베어 보드로 제작된 안테나는 LTE/CDMA/GSM900/DCS/USPCS/WCDMA/WiBro/WiFi 대역에서 VSWR 2.5:1 특성을 만족하였다. 평균 이득과 효율은 LTE, CDMA, GSM-900 대역에서 -3.98~-0.09 dBi 및 40.03~97.99 %가 측정되었다. 또한, DCS, USPCS, WCDMA, WiBro, WiFi 대역에서는 -3.90~-1.01 dBi 및 40.70~79.31 %의 효율 특성을 가짐으로써 LTE를 포함한 휴대폰 전 대역 안테나로 사용될 수 있음을 보였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제33권1호
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• pp.40-45
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• 2007

Objective. The objective of this international comparative study was to investigate the clinical features and outcome of the treatment of infratemporal fossa abscess (IFA). Study design. This retrospective study was conducted at the Deptartment of Oral and Maxillofacial Surgery of Hallym University and "Iuliu Hatieganu" University of Medicine and Pharmacy. Ten-year records of patients were reviewed in Romania and six-year records were reviewed in Korea. The collected data was then analyzed. Results. A total of 36 cases were found to be IFA (12 males and 24 females: average age; $36.3{\pm}15.5$ yrs: 34 cases from Romania and 2 cases from Korea). The annual frequency of IFA in Romanian and Korean hospitals was 3.40 and 0.33 respectively (P<0.001). The etiology was septic anesthesia (33.3%), infection occurring after extraction (30.6%), periapical lesion (13.9%), impacted third molar (8.3%), post-extraction alveolitis (5.6%), and unknown (8.3%). A successful outcome was seen in 27 patients (75.0%) after initial treatment. The main complication after initial treatment was restricted movement of the mouth (9 cases). Conclusion. The etiology of IFA was various and minimal swelling hampered early diagnosis. To prevent IFA, preoperative painting with antiseptic agent must be stressed and proper drainage proved important to relieve pain and to prevent further complications.

• Parasites, Hosts and Diseases
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• 제25권2호
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• pp.188-194
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• 1987

돼지에 $1.5{\times}10^6$개의 Sarcocystis suicanis sporocyst와 10,000개의 Toxcplasma gondii oocyst(미국 Georgia 대학교 수의과대학 보존주)를 각각 경구감염 시킨후 9주간에 걸쳐 혈중 IgG 항체의 소장과 Sarcocystis와 Tohoplasma 사이의 혈청학적 교차반응성을 IFA 검사와 ELISA법을 이용하여 검사하였다. IFA검사와 ELISA를 위한 Sarcocystis의 전충체 항원과 수용성 항원은 감염돈의 확육을 인공소화액으로 소화시켜 준비하였다. Toxoplasma의 전충체 항원과 수용성 항원은 감염 mouse 복강에서 충체를 분리하여 사용하였다. Sarcocystis와 Togoplasma를 각각 인공감염시킨 돈혈청의 IgG 항체는 IFA검사와 ELISA에서 모두 감염 2주째에 처음으로 검출되었으며 Tcxoplasma에 대한 항체가는 감염 6주째에 최고치에 도달하였다가 그 이후에는 하강하였다. Sarcocystis에 대한 항체가는 감염 9주까지 서서히 상승하였다. Toxoplasma에 감염된 돈혈청은 Sarcocystis 항원에 대하여 IFA검사에서는 1 : 16까지 교차반응이 나타났으며 ELISA에서는 1 : 32까지 나타났다. 대조군의 돈혈청은 모두 1 : 4 이하이었다.

• Safety and Health at Work
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• 제13권4호
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• pp.429-439
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• 2022

Background: The risk observatory (RO) of the German Social Accident Insurance (DGUV) provides strategic support to the German Social Accident Insurance Institutions (GSAII) in proactive prevention. It does so by identifying future challenges and opportunities for occupational safety and health (OSH) resulting from new trends and developments that affect employees as well as children in elementary education, pupils, and students. Methods: The core of the RO is an online survey that relies on a pool of new trends and developments identified via internet and literature research. 865 prevention experts of the GSAII and the DGUV participated in the survey. They rated trends and developments regarding their sector-specific risks and opportunities for OSH in the 5 years to come. Results: Sector-specific and over-all results show that besides well-known OSH risks such as musculoskeletal stress and noise, developments relevant for OSH come to the fore that do not have their origin in work itself, but are strongly influenced by political, social, economic, environmental, or technical developments that accident insurance can only peripherally influence. Shortage of skilled staff was identified as a threat to OSH in almost all sectors. Conclusions: Prevention must find ways to address repercussions of such OSH risks. Cooperation and political awareness are therefore gaining in importance. Also, implementing a prevention culture in society and strengthening individuals' health and safety literacy, e.g., by target-group-specific communication and sensitization, as well as early safety and health education, help to counteract those OSH risks.