• 제목/요약/키워드: IEF

검색결과 63건 처리시간 0.031초

증가인자 시변제어를 위한 신경망 증가평가필터 설계 (Design of Neural Network Based IEF Filter for Time-varying Control of Incremental Factor)

  • 박상희;최한고
    • 대한의용생체공학회:의공학회지
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    • 제23권5호
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    • pp.333-340
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    • 2002
  • 생체신호 수집시 전력선 잡음은 일반적인 잡음원이다. 증가평가필터(Incremental Estimation Filter. IEF)는 생체신호, 특히, 심전도 (Electrocadiogram, ECG) 신호에 있어서 전력선 잡음을 제거하기 위해 사용되어 왔다. 증가평가필터의 잡음제거 성능에 영향을 미치는 상수 값의 증가인자는 입력신호에 따라서 경험적으로 혹은 실험적으로 결정되고 있다. 본 논문에서는 증가인자의 시변(time-varying) 제어를 위해 신경망을 이용한 증가평가필터 설계를 제시하고 있다. 제안된 증가평가필터는 인위적인 신호뿐만 아니라 MIT/BIH 데이터베이스의 실제 심전도 신호에 적용함으로써 평가하였으며, 잡음제거 성능의 상대적인 비교를 위해 적응잡음제거기와 기존의 증가평가필터등과 비교하였다. 실험결과 신경망에 근거한 증가평가필터는 수렴속도와 특정 주파수에서의 잡음제거에서 기존의 적응필터보다 우수함을 보여주었다.

Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs

  • Kong, C.;Ragland, D.;Adeola, O.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권8호
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    • pp.1124-1130
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    • 2014
  • The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, $69.1{\pm}6.46$ kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated $5{\times}2$ incomplete Latin-square design. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 12 h on each of d 6 and 7 and between each period, there was a 5-d recovery period to avoid abnormal weight loss. The ratios of corn starch to dextrose investigated were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4 and 5, respectively, and chromic oxide (5 g/kg) was used as an indigestible index. Ileal DM digestibility was greater in Diet 1 than that in Diet 4 (89.5% vs 87.3%, p<0.01) but they were not different from Diet 2, 3, or 5. The IEF for most of indispensable AA were not different among diets with the exception of Met, in which a lack of corn starch or dextrose gave lower (p = 0.028) IEF of Met than diets containing corn starch and dextrose. Likewise, the dispensable AA and total AA in the IEF did not differ among diets. The respective IEF of AA (mg/kg of dry matter intake) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp and together accounted for approximately 50% of the total ileal AA flows of pigs fed NFD. In conclusion, the variation in proportion of corn starch and dextrose in a NFD does not largely affect estimates of IEF of N and AA for growing-finishing pigs.

Evaluation of Mechanical Properties of Highly Porous Titanium Considering its Application as a Biomaterial

  • Schiefer, Herwig;Bram, Martin;Buchkremer, Hans Peter;Stover, Detlev
    • 한국분말야금학회:학술대회논문집
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    • 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part 1
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    • pp.309-310
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    • 2006
  • Porous titanium implants can be produced by powder metallurgy in combination with suitable space holder materials. Various mechanical experiments were done to characterize this material regarding the influence of the processing parameters on microstructure and mechanical properties taking into account the properties of the human bone. In this paper, the anistropic behaviour of uniaxially compacted samples was analysed in compression tests and compared to the behaviour of isostatically pressed samples. The failure of the struts of the porous titanium and the crack- initiation and -growth was examined by in-situ SEM analysis.

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복합음이온 교환섬유의 플라스마 산화 처리한 NO의 흡착특성 (Adsorption Properties of Oxidized NO by Plasma Using Hybrid Anion-Exchange Fibers)

  • 조인희;강경석;황택성
    • 폴리머
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    • 제30권4호
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    • pp.291-297
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    • 2006
  • 본 연구에서는 아민화 polyolefin-g-GMA 복합음이온 교환섬유를 이용하여 플라스마 산화된 NO의 흡착특성을 고찰하였다. 플라스마 산화에 의한 $NO_2$ 전환율은 NO 200 ppm, 산소 10%, 유속 30 L/min 일 때 최대 49% 이었다. 또한 복합음이온 교환섬유의 $NO_2$ 흡착량은 함수율이 높을수록 증가하였고 함수율이 최대 1.5 g $H_2O/g$ IEF 이었으며, 복합음이온 교환섬유의 $NO_2$ 흡착은 10 분까지 빠르게 진행되었고 120 분에서 최대 80% 흡착되었다. 이온교환 용량은 함수율이 증가함에 따라 증가하였으며, 흡착컬럼 충전 비가 L/D=5에서 0.6 mmol/g IEF로 가장 높았다. 또한 이온교환 섬유의 흡착은 Langmuir 등온흡착 모델보다 Freundlich 등온흡착 모델에 가까웠으며, 다분자층에서의 흡착이 우세하게 발생한 것을 확인할 수 있었다.

Genotypic and Geographical Variations of $\beta$amylase Isozyme in Soybean Land Races by Isoelectric Focusing (IEF)

  • Yoon, Mun-Sup;Ahn, Jong-Woong;Kang, Jung-Hoon;Baek, Hyung-Jin;Park, Nam-Kyu;Rho, Yong-Deok
    • 한국작물학회지
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    • 제45권2호
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    • pp.139-142
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    • 2000
  • The experiment was carried out to study the variations and geographical distribution of $\beta$-amylase isozyme by isoelectric focusing (IEF) within Korean, Chinese and Japanese soybean land races. In pH 3-10 gel of IEF, the amylase of soybean accessions was separated into low pI group isozymes (TEX>${$Sp_1$}^b$) and high pI group isozymes(${$Sp_1$}^a$). In pH4-6.5 gel, isoelectric points were at 5.07, 5.15, 5.25, 5.40, and 5.94, and h, j, and k bands also were found. The distribution of Sp$_1$$^{a}$ allele (high pI type) was 29.3% in soybean accessions from Korea, 10.1 % in those from China, and 6.9% in Japanese accessions. The percentage of ${$Sp_1$}^a$) allele was the highest in soybean accessions from Kyungsang province (35 %) in Korea, then central China (32 %) in China, and Honshu (10%) in Japan.

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삼세기(Shaggy sea raven, Hemitripterus villosus)의 carbonic anhydrase III에 관한 연구 (Presence of Carbonic Anhydrase III-like Protein in Shaggy Sea Raven, Hemitripterus villosus)

  • 권록은;고강희
    • 생명과학회지
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    • 제24권2호
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    • pp.186-190
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    • 2014
  • 본 논문에서는 삼세기(Shaggy sea raven, Hemitripterus villosus)를 실험 재료로 선택하여 Carbonic Anhydrase Isozymes (CAs) 중의 하나인 CA III에 대한 연구를 SDS-PAGE, Isoelectric Focusing (IEF), Western blot analysis의 방법을 통하여 진행하였다. SDS-PAGE와 Western blot 결과 삼세기 아가미, 혈액, 장, 간, 신당, 근육, 심장조직에서 CA III의 분자량인 30 kDa의 band가 확인되었다. 삼세기의 근육과 아가미에 대한 등전점 전기영동(IEF)과 Western blot analysis 결과 pI 7.0 부근에서 band가 형성되는 것을 확인할 수 있었다. 특히 SDS-PAGE와 IEF 실험결과 삼세기의 아가미 조직에서 CA III의 발현량이 다른 조직들에 비하여 우세하게 나타났다. 이는 아가미가 다른 조직들과 달리 어류의 생체기관 중 유일하게 외부와 직접 접촉이 가능한 조직으로서 활성산소에 대한 손상을 최소화하기 위한 것으로 사료된다.

Isoelectric Focusing of the Hemolymph Proteins of the Silkworm, Bombyx mori

  • Staykova, Teodora;Popov, Petar;Grekov, Dimitar;Terzieva, Petia
    • International Journal of Industrial Entomology and Biomaterials
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    • 제8권1호
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    • pp.117-121
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    • 2004
  • Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

Identification of Recombinant Subtilisins

  • CHOI , NACK-SHICK;YOO, KI-HYUN;YOON, KAB-SEOG;CHANG, KYU-TAE;MAENG, PIL-JAE;KIM, SEUNG-HO
    • Journal of Microbiology and Biotechnology
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    • 제15권1호
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    • pp.35-39
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    • 2005
  • To identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusingfibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laemmli buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway.

Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat

  • Cho, Seong-Woo;Kwon, Soo-Jeong;Roy, Swapan Kumar;Woo, Sun-Hee
    • 한국작물학회지
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    • 제60권1호
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    • pp.29-32
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    • 2015
  • Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

Electrophoretical Analysis of 36-Kilodalton Outer Membrane Protein of Vibrio vulnificus ATCC 27562

  • Moon-Soo Heo;Cho-Rok Jung
    • Journal of Life Science
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    • 제9권1호
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    • pp.35-39
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    • 1999
  • Elecrophoreticl analysis of a 36 kDa protein was runned by SDS-PAGE, isoelectric focusing (IEF) and two dimensional electrophoresis pattern. Major 36 kDa and 25, 46, 48, 66 kDa protein were detected by Coomassie blue stain on SDS-PAGE. Major 36kDa protein was eluted for production of antiserum for serological analysis, IEF and two dimensional electrophoresis. Isoelectric point of 36kDa was aout pH 8.5. Two dimensional electrophoresis of eluted 36kDa showed one point on the gel. Anti-36 kDa serum made by newzilland rabbit for serological test. In ELISA, final titer of antibody was 100×{TEX}$2^5}${/TEX} : 1. Neutralize ability of serum was examined by slide agglutination test and colonization test in rat. Anti-36 kDa serum agglutinated whole cell of V. vulnificus were inhibited colonization on intestine in rat. Accordingly In this paper contain some electrophoretical analysis and serological test of a 36 kDa OMP of V. vulnificus.

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