International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Isoelectric Focusing of the Hemolymph Proteins of the Silkworm, Bombyx mori

  • Staykova, Teodora (Department of Genetics, Plovdiv University) ;
  • Popov, Petar (Plovdiv University, Department of Genetics) ;
  • Grekov, Dimitar (Agricultural University Plovdiv, Department of Animal Husbandry) ;
  • Terzieva, Petia (Research Institute of Fisheries, Laboratory of Biochemical and Population Genetics)
  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

Keywords

References

  1. Banno, Y., S. Tochihara, Y. Kawaguchi and H. Doira (1993) Hemolymph proteins during larval development of Bombyx mori: 'Proteins of young larvae'. J. Seric. Sci. Jpn 62, 187-194
  2. Banno, Y., N. Kazuhiro, Y. Kawaguchi, K. Koga and H. Doira (1994) Linkage studies on a haemolymph protein named 'Protein of young larvae A4 (PYL-A4)' of the silkworm Bombyx mori. J. Seric. Sci. Jpn 63, 299-302
  3. Eguchi, M. (1968) Changes in zymograms of some dehydrogenases and hydrolases in several organs of the silkworm Bombyx mori L. (Lepidoptera: Bombycidae) during metamorphosis. Appl. Entomol. Zool. 3, 189-197 https://doi.org/10.1303/aez.3.189
  4. He, J. L. (1995) Studies on the inheritance of a deletion type of the blood esterase isoenzymes in the silkworm Bombyx mori L. Sericologia 35, 17-24
  5. Kanekatsu, R, M. Satoh, R Kodaira and T. Miyashita (1993) Midgut sucrase-I (suc-l ) of the silkworm, Bombyx mori genetics and changes in the activities during the pupal-adult development. J. Seric. Sci. Jpn 62, 13-19
  6. Kawaguchi, Y., Y. Banno, K. Koga, H. Doira and H. Fujii (1993) Characteristic profiles of haemolymph proteins during larval development of molting mutants of the silkworm Bombyx mori. Comp. Biochem. Physiol. 105 B, 361-367
  7. Krishnamurthy, N. B., S. R Ramesh and M. R. Ragasekarrty (1984) Developmental profiles in the isozymes of ($\alpha$- and $\beta$esterases in the embriogenesis of silkworm Bombyx mori, Curr. Sci. (India) 53, 281-284
  8. Lee, S. M., K. E. Park and J. Y. Moon (1985) Studies on the varietal differences of the electrophoretic protein patterns in the pupal haemolymph of the silkworm Bombyx mori. Korean J. Seric. Sci. 26, 35-51
  9. Lee, S. M., S. I. Seong, H. A. Kang and J. Y. Moon (1992) Physiological and biochemical studies on the adult haemolymph proteins in Lepidoptera. II. Purification and molecular properties of adult specific protein in the silkworm Bombyx mori. Korean J. Seric. Sci. 34, 30-34
  10. Lee, S. M., S. I. Seong, J. Y. Moon and H. A. Kang (1994) Physiological and biochemical studies on the adult haemolymph proteins in Lepidoptera. In vivo and in vitro synthesis of adult major haemolymph protein in the silkworm, Bombyx mori. Korean J. Seric. Sci. 36, 119-123
  11. Lowry, O., N. Rosebrough, A. Faar and R. Randaal (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
  12. Marcato, S., M. Trevisan, L. Cappellozza and P. Bisol (1990) Variabilita di sistemi gene-enzima, in razze di Bombyx mori (Lepidoptera, Bombycidae) utilizzate per la produzione di seta. Estratto da Redia LXXIII, 595-608
  13. Ostapenko, V., T. Egorova, Y. Philippovich and G. Ushakova (1975) Comparative description of the original breeds of highly heteroses hybrids of silkworm in soluble proteins of eggs and hemolymph. Genet. Breed. 6, 102-109
  14. Philippovich, Y. and M. Alieva (1966) Protein fractions in the Bombyx mori L. hemolymph according to the stage development and sex. J. Gen. Biol. 27, 489-495
  15. Philippovich, Y., S. Klunova, V. Evtushenko and M. Kumanov (1978) Studies on the enzymes amino acid interchange in tissues and organs of the silkworm in race hybrids and specimen aspects. Annu. Mid. Asian Res. Inst. Silkworm-Breeding (SANllSH) 12, 48-59
  16. Raju, P. and N. Krishnamurthy (1995) Ontogenetic differentiation of alpha and beta esterase in univoltine, bivoltine and trimoulter races of silkworm. Ann. Trop. Res. (Philippines) 17, 45-53
  17. Seong, S. I. (1981) Changes of proteins and nucleic acids during metamorphosis in the midgut of the silkworm Bombyx mori (Lepidoptera: Bombycidae). Appl. Entomol. Zool. 16, 138-148 https://doi.org/10.1303/aez.16.138
  18. Seong, S. I., K. E. Park, M. Nagata and N. Yoshitake (1985) Effect of metamorphosis on the major haemolymph proteins of the silkworm. Arch. Insect Biochem. Physiol. 2, 91-104 https://doi.org/10.1002/arch.940020109
  19. Seong, S. I., J. Y. Moon, S. M. Lee and H. J. Yoon (1988) Physiological and biochemical studies on the adult hemolymph proteins in Lepidoptera. 1. Appearance of adult specific proteins in the hemolymp of the silkworm Bombyx mori. Korean J. Seric. Sci. 30, 20-24
  20. Seong, S. I. (1991) Change in soluble and insoluble proteins during embryonic development of the silkworm, Bombyx mori. Korean J. Seric. Sci. 33, 1-5
  21. Shimada, T., M. Kobayashi and N. Yoshitake (1985) Genetical analysis of storage proteins in the silkworm, Bombyx mori. J. Seric. Sci. Jpn 54, 464-469
  22. Stoykova, T. and P. Terzieva (1998) Ontogenetic electrophoretic studies of the proteins in the hemolymph of mulberry silkworm Bombyx mori L. (Lepidoptera, Bombicidae). Acta Zool. Bulg. 50, 147-151
  23. Stoykova, T., P. Popov, D. Grekov and M. Panayotov (1998) Genetic control of nonspecific esterases in mulberry silkworm (Bombyx mori L.) silk glands during ontogenesis. Sericologia 38, 237-242
  24. Stoykova, T., P. Popov and D. Grekov (1999) Electrophoretic studies of water soluble proteins of mulberry silkworm (Bombyx mori L.) testes during ontogenesis. Sericologia 39, 505-509
  25. Stoykova, T. (2001) Electrophoretic analysis of nonspecific esterases in silkworm (Bombyx mori L.) fat body during ontogenesis. Sericologia 41, 1-13
  26. Xu, J. F. and H. S. Song (1987) A study on esterase isozyme of silkworm (Bombyx mori) embryo. Sericologia 27, 309-314