• 미생물학회지
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• 제31권1호
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• pp.72-78
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• 1993

Human cytomegalovirus (HCMV) 의 immediate early(IE) 유전자의 promoter/enhancer 부위에 cAMP response element가 있다는 것으부터 caMP 가 HCMV 의 증식에 관여할 것이라고 생각할 수 있다. 이러한 가능성을 알아보기 위해 8-bromoadenosine 3', 5'-cyclic monophosphate (BrA) 와 papaverine 같은 세포내 cAMP 농도를 변화시키는 약제를 사용하여 HCMV 의 증식, DNA 합성 및 IE 유전자 발현에 대한 영향을 알아보았다. HCMV 증식과 DNA 합성은 papaverine 에 의해 억제된 반면, BrA 는 HCMV 의 증식에는 큰 영향을 주지 않았고 DNA 합성은 오히려 촉진시키는 것을 알 수 있었다. HCMV IE promoter 에 의해 작동되는 CAT 유전자를 함유한 plasmid pCMVIE/CAT 을 세포내로 transfection 시켰을 때, papaverine 을 처리한 세포에서는 CAT 효소 활성도가 감소한 반면 BrA 를 처리한 세포에서는 증가하였다. HCMV 에 감수성이 없는 HeLa 세포에서는 CAT 활성도가 감소성 세포인 HEL 세포에서보다 높게 나타난 반면, Vero 세포에서는 낮게 나타났다. 이들 비감수성 세포인 HEL 세포에서 보다 높게 나타난 반면 Vero 세포에서는 낮게 나타났다. 이 들 비감수성 세포에서의 CAT 활성도는 BrA 를 처리하여 주었을 때 모두 증가하였다. 이상과 같은 결과로부터 cAMP 는 HCMV 증식과 IE 유전자 발현에 어느 정도 관여한다는 것을 알 수 있다.

• 한국지반공학회논문집
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• 제18권4호
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• pp.271-283
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• 2002

콘크리트 구조물의 건전성 평가에 사용되는 충격반향기법에서는 콘크리트의 P파 속도가 부재의 두께 및 결함의 위치를 찾는데 있어서 매우 중요한 요소이다. 본 논문의 동반논문인 수치해석편에서 표면파기법을 이용하여 콘크리트 부재의 P파 속도를 신뢰성 있게 추정함을 알 수 있었다. 수치해석 연구에 이어서 본 논문에서는 공극, 불량콘크리트 등의 결함이 내재된 콘크리트 모형 슬래브를 제작하여 제안된 충격반향-표면파 병행기법의 실제 구조물에 대한 적용성 연구를 수행하였다. 실험결과, 표면파기법으로부터 구한 부재의 P파 속도는 두께를 알고 있는 부분에서 충격반향기법으로부터 구한 P파 속도와 거의 일치하였으며, 표면파기법으로부터 구한 P파 속도를 적용하여 충격반향기법을 수행한 결과, 여러 결함의 위치를 신뢰성있게 찾아낼 수 있었다. 또한, 실제 지중에 매설되는 관 형태의 지중 구조물과 지중에 매설된 개착식 터널에서 제안된 비파괴검사기법을 수행한 결과, 구조물의 두께를 신뢰성 있게 구하여 실제 지반구조물에서의 현장 적용성을 확인하였다.

• 대한바이러스학회지
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• 제29권2호
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• pp.137-144
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• 1999

Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.

• 지구물리와물리탐사
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• 제12권4호
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• pp.338-346
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• 2009

콘크리트의 배면에 위치하는 공동을 탐지하기 위해 실제 구조물과 같은 스케일의 모형을 제작하여 비파괴 검사로 널리 사용되는 GPR (ground penetrating radar)탐사와 충격반향기법(IE; impact echo), 충격응답기법(IR; impulse response)을 복합적으로 적용하였다. 콘크리트 모형은 철근 및 공동의 유무에 따라 다양한 반응을 관찰할 수 있도록 설계 하였다. GPR 탐사결과 공동의 유무에 따라 반응의 차이가 나타났지만 정확하게 공동의 존재를 파악하기 어려웠으며, 주로 건전도 측정을 위해 지반조사에 활용되었던 IE 및 IR 기법은 공동의 유무를 구분할 수 있는 반응을 보여주었다. 각 탐사의 적용 결과 철근의 존재에 따라 각 방법별로 배면의 공동을 탐지하는데 반응의 변화가 나타나긴 했지만, 세 가지 방법을 복합적으로 적용하였을 때 단일 탐사를 이용한 결과보다 정확하게 배면 공동의 위치를 파악할 수 있었다.

• 산업공학
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• 제20권1호
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• pp.1-10
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• 2007

The main purpose of this research is to find out whether curriculums of industrial engineering (IE) departments meet the demand of IE graduates working in various fields. The research was conducted as an online questionnaire survey selecting IE Graduates working in industries as practising engineers. 1,324 participants were validated among 1,477 participants. 13 fields were selected and used in the survey. Those were; 1) Mathematical statistics, 2) Computer, 3) Purchase, 4) Production system, 5) Logistics, 6) Marketing, 7) Monetary, 8) Experiment methods, 9) Operations Research (OR), 10) Human Factors, 11) Quality, 12) Engineering management, and 13) Information systems. Using the 5-scale Likert rating, each education subject was assessed both in terms of its usefulness in practices and the amount it being taught in school. As a result, courses such as motion/time study, linear programming that IE has traditionally focused showed less usefulness in practices while it is taught in relatively large amount in schools. However, courses such as 6 sigma, CRM which are closely related to industrial practices showed high usefulness in practices compared with low degree of teaching in school. This was the first ever large scalesurvey conducted for IE graduates in Korea. The result of survey displayed many helpful information on current status and future direction of IE education in Korea.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.43-49
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• 2001

We have cloned and characterized an immediate early-1 gene, iel, which is activated immediately upon entrance of the viral genome into the cell nucleus, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. This gene encodes a protein 584 amino acids with a predicted molecular weight of 67 kDa. The promoter and coding regions of BmNPV-K1 ie1 showed high homology with Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 ie1 was different from amino acid sequence at 4 positions in BmNPV T3. The location of ie1 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were confirmed by Nerthern hybridization analysis.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.131-135
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• 2007

Open reading frame 4 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm4, is a gene whose function is completely unknown. With the recently developed BmNPV bacmid and a modified pFastBac1 whose polyhedrin promoter was replaced with ie1 promoter, a recombinant bacmid expressing Bm4-EGFP fusion protein under the control of ie1 promoter in BmN cells was successfully constructed. The result not only showed that the polyhedrin promoter can be replaced efficiently with other promoters to direct the expression of foreign gene in BmN cells by using Bac-to-Bac/BmNPV baculovirus expression system but also laid the foundation for rescue experiment of Bm4 deletion mutant due to the ability of ie1 promoter to direct gene expression throughout the infection cycle.

• 조명전기설비학회논문지
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• 제29권11호
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• pp.35-40
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• 2015

In accordance with global energy conservation policies such as MEPS (Minimum Energy Performance Standard), electric motor industry is moving to super-high-efficiency machines and research to develop IE4 (International Energy Efficiency Class 4) motors has been launched. In this situation, SynRM (Synchronous Reluctance Motor) has been attracting attention in place of induction motor which hardly provides super premium efficiency. As a result, much research on SynRM is being performed at home and abroad. Also, some products have already been appearing in the market. Compared to induction motor, SynRM has better efficiency per unit area and wider operating range. Although the utilization of control system in synchronous motor results in higher prices, we still need to concentrate on developments of SynRM so as to comply with the new policies. This study demonstrated the electromagnetic design methods of super premium SynRM while maintaining the frame of existing IE3 induction motor. We documented the design procedures for generating high saliency which is the most essential and mechanical stress anlaysis is also treated. In conclusion, we proved the validity of our design by manufacturing and testing our 3 models.

• Journal of Electrical Engineering and Technology
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• 제12권2호
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• pp.803-808
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• 2017

Minimum Energy Performance Standard(MEPS) has been adapted in Korea since 2008. The efficiency standard of variable speed motors is scheduled for publication shortly. In this paper, author reviews the technical trends associated with a high efficient electric motors by reference to research papers, and reviews the research direction to prepare for IE5. This paper provides an overview of high efficiency motors. Various applications have been covered.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2003년도 봄 학술발표회 논문집
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• pp.309-314
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• 2003

Impact Echo method has been successful in detecting a variety of defects in concrete structure. This study has the objectives to show important aspects of applying the Discrete Wavelet Transform(DWT) to signal processing of Modified Impact Echo(ModIE) Measurement systems and to the understanding of the seismic wave propagation. The data of ModIE were processed by DWT and compared with the results of conventional ModIE Analysis. Although it is inconsistent in the evaluated thickness of concrete lining, the DWT provides the features of separation, synthesis and de-noising in the original signal. The application of technique by wavelet was explained numerically with ABAQUS and performed experimentally with a real scale model in this work. Further works on the possible ways for creating new mother wavelet are specially needed for the enhancement of seismic signal analysis.