The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Characterization of Insect Cells Transformed with Autographa californica Nuclear Polyhedrosis Virus IE1 Gene

Autographa californica 핵다각체병 바이러스의 IE1 유전자에 의해 형질전환된 곤충세포주의 특성

  • Cho, Eun-Sook (College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Hae-Jin (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Kang, Seok-Woo (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology, RDA) ;
  • Yun, Eun-Young (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Keun-Young (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology, RDA) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • 조은숙 (동아대학교 생명자원과학대학) ;
  • 박혜진 (동아대학교 생명자원과학대학) ;
  • 이광식 (동아대학교 생명자원과학대학) ;
  • 강석우 (농업과학기술원 잠사곤충부) ;
  • 윤은영 (농업과학기술원 잠사곤충부) ;
  • 김근영 (농업과학기술원 잠사곤충부) ;
  • 손흥대 (동아대학교 생명자원과학대학) ;
  • 진병래 (동아대학교 생명자원과학대학)
  • Published : 1999.06.30
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Abstract

Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.

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