• Journal of Microbiology
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• 제45권6호
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• pp.566-571
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• 2007

Bacterial lipopolysaccharide (LPS) is a potent stimulator of bone resorption in periodontitis. Co-culture systems of mouse calvaria-derived osteoblasts and bone marrow-derived preosteoclasts were used as an in vitro osteoclast differentiation. This study revealed that co-cultures using ddY or ICR mouse strain responded differently to LPS while responded equally to $1{\alpha},25(OH)_2D_3$. Thus, the different response to LPS indicates dissimilarity of two mouse stains in their capacity for generating osteoclasts while the two mouse strains share the similarity in response to $1{\alpha},25(OH)_2D_3$. To identify which cells between osteoblasts and preosteoclasts in the co-culture are responsible for the dissimilarity, the reciprocal co-cultures were performed between ddY and ICR mouse strains. The treatment of $1,25(OH)_2D_3$ to ddY/ICR (osteoblasts from ddY/preosteoclasts from ICR) and ICR/ddY reciprocal co-cultures also showed the similarity. In case of LPS treatment, the results of ddY/ICR were similar to ddY/ddY and the results of the other reciprocal co-culture, ICR/ddY combination, were consistent with those of ICR/ICR. It suggests that the dissimilarity between the two mouse strains may resident in osteoblasts but not in preosteoclasts. Therefore, the osteoblast is responsible for mouse strain-dependent osteoclastogenesis in response to LPS. Although mouse models will continue to provide insights into molecular mechanisms of osteoclastogenesis, caution should be exercised when using different mouse strains, especially ddY and ICR strains as models for osteoclast differentiation.

• 농업과학연구
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• 제5권2호
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• pp.77-79
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• 1978

본(本) 연구(硏究)는 5계통(系統)(CF#1, C57BL/6, ICR, dd, CHN)의 mouse에 있어서 과배란처리(過排卵處理)에 대한 반응차(反應差)를 조사(調査)하기 위하여 수행(遂行)되었다. 과배란처리(過排卵處理)는 5IU의 PMS를 피하(皮下)에 주사(注射)한 다음 58시간(時間) 후(後)에 5IU의 HCG를 피하주사(皮下注射)하였으며, 배란수(排卵數)의 조사(調査)는 HCG 주사(注射) 24시간후(時間後)에 도살(屠殺)하여 난관내(卵管內)의 난자수(卵子數)를 현미경하(顯微鏡下)에서 계정(計定)하였다. 평균배란수(平均排卵數)는 CF#1, C57BL/6, ICR, dd 및 CHN계통(系統)에서 각각 21.3, 17.9, 20.7, 21.0 및 20.5개였다.

• Toxicological Research
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• 제7권1호
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• pp.47-50
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• 1991

Hematological studies in two strains of normal laboratory mice, namely ICR and ddY which were produced in Koren were carried out. Blood analysis was performed by using different methods, a fully automated analyzer (ELT 1, 500), Coulter counter (model S plus IV) and manual technique to examine possible differences resulting from methodolgies'slight differences among the values of blood parameters were obtained. There was a slight difference noted between sexes, however, similar values were obtained from both strains.