• Journal of Applied Biological Chemistry
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• v.56 no.1
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• pp.53-57
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• 2013

In here, we investigated the quantitative analysis method of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with liquid chromatography-mass/mass spectrometry (LC-MS/MS) or gas chromatography-MS. Two ways of clean-up process were investigated for LC-MS/MS instrumental analysis of IAA, but both a simple dilution and hydrophile-lipophile balance (HLB) solid phase extraction (SPE) were not met the optimal recovery rates for quantitative analysis. On the other hand, the clean-up method for GC-MS was finally optimized through HLB-SPE from 250-folds diluted sample and methylation with trimethylsilyl chloride in methanol for 4 h. The limit of detection for methyl ester of IAA and IBA were both 1.4 mg/L, and recovery rates showed 93-107% from the concentrated liquid fertilizer.

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.145-155
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• 2015

To investigate the practicality of a small scale softwood propagation plastic film tent and the rooting promoting effect of indole-3-butyric acid(IBA) for northern highbush blueberry, softwood cuttings of 3 varieties (Duke, Elizabeth, Elliot) were made after dipping in the IBA solutions of 100ppm, 300ppm, 500ppm, 1,000ppm for 2 hour, and 3,000ppm for 20 seconds, respectively. Two-way ANOVA confirmed the significant differences in the percentage of rooting of cutting, longest root length and rooting volume index (RVI) induced by IBA treatment, as well as the percentage of rooting of cutting and RVI among examined varieties. In particular, the highly significant difference was observed in th RVI for IBA treatment. However, there was no interactions between the auxin treatments and varieties. Spontaneous rooting ability (control) depends on the varieties, showing that the lowest rooting ability was observed in the 'Duke' (45%) followed by 'Elliot'(85%) and 'Elizabeth' (90%). In addition, the rooting of the examined 3 varieties were enhanced by both of short time (20sec) dipping treatment of low level solutions, and long time (2hr) dipping treatment of high level solution, with the remarkably promoted rooting of the 'Duke' cuttings.

• Proceedings of the Botanical Society of Korea Conference
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• 1995.06a
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• pp.12-26
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• 1995

• Journal of Sericultural and Entomological Science
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• v.32 no.1
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• pp.38-43
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• 1990

Effects of topical application of 100, 200 and 300ng/$m\ell$ of 3-indole butyric acid (3IBA) on larval parameters were studied in polyvoltine Pure Mysore breed of B. mori. Each concentration was administered independently, at 36h to III, IV ＆ V instars, at 48h to IV ＆ V instars, and at 72h to V instar larvae. Of the various dosages used, the repeated applications of loons of 3-IBA at 36h to III, IV ＆ V instars resulted in a significant increase in larval weight, silk gland weight, cocoon shell weight and fecundity and a significant decrease in larval duration and cocooning percentage when compared with the corresponding parameters of the untreated controls.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.205-210
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• 1994

This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

• Mycobiology
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• v.37 no.1
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• pp.28-30
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• 2009

This study was aimed at evaluating the growth promoting effect of symbiotic algal polyol (ribitol) and plant hormones on the lichen-forming fungi (LFF), Ramalina farinacea (CH050010 and 40403) and Ramalina fastigiata. The addition of ribitol to basal (malt-yeast extract) medium enhanced the relative growth rates of all three LFF. R. farinacea (CH050010), R. farinacea (40403) and R. fastigiata (H06127) showed 35.3%, 29.0% and 29.3% higher growth rates, respectively, compared to the control. IBA (indole-3-butyric acid) and TIBA (2,3,5-tridobenzoic acid) also increased growth rates of the LFF by 34 to 64% and 7 to 28%, respectively, compared to the control. The combination of ribitol with IBA or TIBA synergistically increased the growth of all LFF. For example, ribitol and IBA treatments increased growth rates of R. farinacea (CH050010), R. farinacea (40403) and R. fastigiata (H06127) by 79.4%, 40.3% and 72.8% in, respectively, compared to those grown on the basal medium. The stimulating effect of ribitol and IBA on the LFF growth induced vertical development of the fungal mass in culture. We suggest that lichen-forming fungal growth of Ramalina lichens can be stimulated aposymbiotically by supplementing polyols and plant hormones to the basal medium in the mass production of lichen secondary metabolites under large scale culture conditions.

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.157-161
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• 2010

Photosynthetic purple nonsulfur bacterial strains were isolated from the sediments collected from rice paddy fields and sludges of wastewater treatment plant, and their plant growth promoting capabilities were examined. Most well known phytohormones, auxin such as indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) and 5'-aminolevulinic acid (ALA) were detected by HPLC in the culture broth of these isolates. Among the isolated bacteria, Rhodopseudomonas faecalis D15 showed the highest production rate of 769.8 ${\mu}g$/mg protein of IAA, 1323 ${\mu}g$/mg protein of IBA and 7.4 mM/mg protein of ALA in the modified Biebl and Pfennig's medium. R. faecalis C9 showed the highest production rate of 20.82 ${\mu}g$/mg protein of gibberellin. In consequence, the root length and dry weight of the germinated tomato seedling treated with R. faecalis isolates were longer and heavier than those of uninoculated control after 15 days of incubation in the soil. Especially, the dry weight of germinated tomato seedling increased by 119.4% in C9-treated samples after 15 days. These purple nonsulfur bacteria may be utilized as environment-friendly biofertilizer in the agriculture.

• Journal of Bio-Environment Control
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• v.30 no.1
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• pp.56-64
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• 2021

This study was conducted to examine the effect by different types and concentrations of auxin on the rooting and growth of strawberry (Fragaria × ananassa Duch. cv. Maehyang) cuttings in the greenhouse. The NAD (1-naphthylacetamide), IBA (indole-3-butyric acid), and IAA (3-indoleacetic acid) were applied with a 1 hour soaking as 50, 100, 150, and 200 mg·L-1, respectively. The non-treatment was set as the control. The cuttings of strawberry were transplanted in the strawberry seedling tray filled with coir medium on June 4, 2020. The humidification was carried out for 2 weeks. The average relative humidity, daytime temperature, and nighttime temperature inside the humidification tunnel was 63.4 ± 15%, 29.3 ± 5℃, and 16.2 ± 5℃, respectively. There was no significant difference in rooting rate on the control, IBA, and IAA treatments. However, significantly low rooting rates were observed in NAD treatments. The survival rates were significantly higher in the control and IBA with 50 mg·L-1 than in other treatments. The number of leaves was the highest in IBA with 100 mg·L-1. The root length was the longest in the control. More number of roots were counted in IAA with 100 and 150 mg·L-1. The dry weight of root was the heaviest in the control. The total root length, root surface, number of root tips, and number of root forks were significantly higher in the control. As a result, control, IAA, and IBA showed similar shoot and root growth. However, NAD showed the worst root and shoot growth. Consequently, compared with IAA and IBA, NAD was not appropriate plant growth regulator of rooting for cutting propagated strawberries.

• Journal of Korea Foresty Energy
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• v.20 no.2
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• pp.34-40
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• 2001

This study was carried out to investigate the effects of IBA(indole-bufrric acid) on the rooting and root growth of air-layered part of Pinus thunbergii for miniature tree material. The experiment was performed on April 13 and lune 11, 2000 in the humidity controllable greenhouse condition. In April experiment, IBA treatment with 5000ppm at girdling part shortened about 20 days for rooting than those of control and 1000ppm treatment. IBA treatment in June did not affect on the period required for rooting, but the rooting of all the air-layered seedlings was faster than that of in April. On the other hand, IBA treatment in April did not affect on the number of root grown at girdling part and the root biomass, but the treatment in June increased the number of root and the root biomass of air-layered Pinus thunbergii.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.35-43
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• 2011

This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with $4.4{\mu}M$ benzylaminopurine (BA) and $2.32{\mu}M$ kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with $13.2{\mu}M$ BA, $2.32{\mu}M$ Kin, and $0.98{\mu}M$ indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with $9.8{\mu}M$ IBA, $2.85{\mu}M$ indole-3-acetic acid (IAA), $2.68{\mu}M$ naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.