• Microbiology and Biotechnology Letters
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• v.19 no.6
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• pp.575-582
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• 1991

To increase the production of glutathione by the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were isolated and cloned. To clone a gshI gene, the GS903 mutant strain, which is deficient in $\gamma$-glutamylcysteine synthetase activity, has been raised. A gshI gene was cloned using pBR322 plasmid as a 3.6 Kb PstI DNA fragment isolated from E. coli K-12 chromosomal DNA. Also a gshIl gene was cloned using pUC13 plasmid as a 2.2 Kb PstI-BamHI DNA fragment. To study the effects of plasmid copy number and passenger DNA size on the expression levels of the gsh genes, various recombinant plasmids containing different sets of genes were constructed. The expression levels of the gsh genes were increased approximately twice higher in pUC series plasmids than that in pBR322 plasmid. But the sizes of the passenger DNA containing the gsh genes in the vector plasmid did not affect on the expression levels of the gsh genes.

• Environmental Mutagens and Carcinogens
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• v.22 no.1
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• pp.39-46
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• 2002

Obesity is a complex metabolic disorder with a strong genetic component. There are many candidate genes for obesity and its related phenotypes. We studied genetic variations between Korean obese and lean groups. Polymorphisms investigated were the Msp I polymorphism of the $\alpha$$_{2A}$-adrenergic receptor ($\alpha$$_{2A}$-AR) gene, the Mnl I polymorphism of the $\alpha$$_2$-adrenergic receptor ($\alpha$$_2$-AR) gene, the BstO I polymorphism of the $\beta$$_3$-adrenergic receptor ($\beta$$_3$-AR) gene, the Pml I polymorphism of the lamin A/C (LMNA) gene, the Hga I polymorphism of the clearance receptor (NPRC) gene, the Msp I polymorphism of the leptin gene, BclI polymorphism of the uncoupling protein 1 (UCPI) gene and the Hha I polymorphism of the fatty acid binding protein 2 (FABP2) gene. Among these genetic markers, Pml I polymorphism at the LMNA gene and Bcl I polymorphism at the UCP1 gene were significantly associated with obesity. However, further studies are required whether thease findings are reproduced in large population, although two polymorphisms might be useful as genetic markers in the ethiology of obesity in Korean population.ion.

• Journal of the Korean Society of Propulsion Engineers
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• v.18 no.1
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• pp.91-96
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• 2014

KSLV-I (Korea Space Launch Vehicle-I) upper stage KM (Kick Motor) is a solid propulsion system which consists of igniter, SAD (Safety Arming Device), composite case, and submerged nozzle capable of TVC (Thrust Vector Control) actuation. Each subsystem of KM fulfilled development requirements for achieving a flight mission successfully. We confirmed the successful development of KM from the $3^{rd}$ flight test results of NARO on January 30, 2013. This article deals with the requirements of KM and the results on configuration management, mass variation, thrust axis alignment, and major test results and so on.

• The Korean Journal of Mycology
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• v.32 no.2
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• pp.148-151
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• 2004

This study was carried out to elucidate phylogenetic relationship of a yellow lump, Phellinus linteus by comparing the nuclear ribosomal intergenic spacer (IGS) I region with that of other genera of basidiomycetes retrieved from Genbank. IGS I region of Phellinus linteus was 730 bp long and sequence homology was conserved in the 5' region, in particular $1{\sim}280\;bp$, and decreased in the direction toward the 3' end. ITS region was widely studied in phylogenies related to basidiomycetes, but IGS region was not well understood yet. Our study indicated that IGS region can be a good tool in phylogenetic study of basidiomycetes.

• Journal of Oriental Medical Thermology
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• v.3 no.1
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• pp.15-19
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• 2004

Cold hypersensitivity means a condition feeling cold at a temperature when one should not feel so. Cold hypersensitivity has been diagnosed based on the patient's subjective complaints. But these days, cold hypersensitivity can be diagnosed by the help of D.I.T.I.(Digital Infrared Thermal Imaging) This study is designed to verify the validity of diagnosis of cold hypersensitivity by D.I.T.I. Thermographic findings of 100 outpatients who visited the Kangnam Korean Hospital, KyungHee University, Seoul, Korea, from June 2000 to November 2000 were analyzed. The experimental group consisted of 50 patients who complained cold hypersensitivity, and for the control group, 50 patients who did not complain cold hypersensitivity. For the diagnosis of cold hypersensitivity of the hands, thermographic measurements were performed on two pairs of areas(palm-upper arm and back of hand-upper arm). And for that of the feet, other two pairs of areas(anterior thigh-top of the feet and posterior thigh-heel). When the criterion for thermal deviation between the palm and the upper arm was appointed as higher than $0.3^{\circ}C$, the sensitivity was 94.0% and the specificity, 90.0%. And when the criterion for that between the anterior thigh and the top of feet was appointed higher than $2.0^{\circ}C$, the sensitivity was 94.0% and the specificity, 76.0%. Through this study, the diagnostic standard for cold hypersensitivity of hands and feet could be presented.

• Current Research on Agriculture and Life Sciences
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• v.7
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• pp.153-163
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• 1989

The activity changes and biochemical properties of ${\beta}$-gal in tomato fruits during ripening were investigated. The total activity was increased during ripening and three isoenzymes (${\beta}$-gal I, II and III) were purified through DEAE Sephadex A-50 and Sephadex G-100 column chromatography. The activities of ${\beta}$gal isoenzymes (${\beta}$-gal I, II and III) during ripening were 69.8, 31.8 and 170.0 units in mature green phase, while those were 48.7, 88.4 and 136.8 units in Red phase, respectively. As the ripening proceeded the activities of ${\beta}$-gal I and III were some what decreased but the activity of ${\beta}$-gal II was incresed more than 2.8 fold. The optimum pH of ${\beta}$-gal I, II and III were 3.9, 4.2 and 3.9 and the optimum temperature of those were $60^{\circ}C$, $56^{\circ}C$ and $60^{\circ}C$, respectively. All isoenzymes were stable at pH 3.6~6.0 and lost their activity about 50% when it heated at $55^{\circ}C$ for 5 minute. $Mg^{{+}{+}}$-activated the three isoenzymes but $Ca^{{+}{+}}$ and SDS inhibited about 30~40%. $Hg^{{+}{+}}$ inhibited completely. The km value of ${\beta}$-gal I, II and III was 0.36mM, 0.63mM and 0.45mM, reaction rate was rapidly increased until the concentration of substrate was $6.0{\times}10^{-5}M$.

• Kyungpook Mathematical Journal
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• v.51 no.1
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• pp.69-76
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• 2011

In this paper, we introduce a partitioning ideal of a ternary semiring which is useful to develop the quotient structure of ternary semiring. Indeed we prove : 1) The quotient ternary semiring S/$I_{(Q)}$ is essentially independent of choice of Q. 2) If f : S ${\rightarrow}$ S' is a maximal ternary semiring homomorphism, then S/ker $f_{(Q)}$ ${\cong}$ S'. 3) Every partitioning ideal is subtractive. 4) Let I be a Q-ideal of a ternary semiring S. Then A is a subtractive ideal of S with I ${\subseteq}$ A if and only if A/$I_{(Q{\cap}A)}$ = {q + I : q ${\in}$ Q ${\cap}$ A} is a subtractive idea of S/$I_{(Q)}$.

• Natural Product Sciences
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• v.4 no.1
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• pp.23-25
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• 1998

Two samples of the fruits of Piper longum have been chemically investigated. From one sample a new alkamide, pergumidiene and from other sample two rare alkamides, brachystamide B and piperderdine have been isolated. The known compounds, piperine, piperlonguminine, pellitorine, (+)-sesamine and 3-(3', 4', 5'-trimethoxyphenyl)-propionic acid were the common constituents of both the samples.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.05a
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• pp.100-100
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• 2002

2-amino-3-methylimidazo[4,5-f]quinoline (IQ), a heterocyclic amine, significantly inhibits nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated macrophages. The decrease in NO production was found to correlate well with a decrease in iNOS mRNA expression as demonstrated by Northern blot analysis.(omitted)

• Journal of the Korean Data and Information Science Society
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• v.25 no.3
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• pp.643-653
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• 2014

In this paper, we propose some estimators for the extreme value distribution based on the interval method and mid-point approximation method from the progressive Type-I interval censored sample. Because log-likelihood function is a non-linear function, we use a Taylor series expansion to derive approximate likelihood equations. We compare the proposed estimators in terms of the mean squared error by using the Monte Carlo simulation.