• Journal of Periodontal and Implant Science
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• 제33권2호
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• pp.193-213
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• 2003

The purpose of this study is to evaluate the phenomenon of attachment and spreading of the cultured rat　calvarial cell inoculated on their surface of different kinds of biodegradable membrane which had been used on tissue regeneration on periodontal defects by using scanning electron microscope. In this experiment 30 Sprague-Dawley male rats (mean BW 150gm) were used to harvest abundant number of cell in the short period. The rats were sacrificed by decapitatioan to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Biodegradable barrier membrane were collected with collagen type, and were divided into 3 different kind of surface such as scattered, polarized and fine-net type as their surface texture. Microcover plate which usually used for cell culture was used as control for smooth surface. All the membrane were seeded with cultured calvarial cell on their surface. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. After the culture as designed time, all the membrane were washed with 0.1 M Phosphate Buffered saline and fuxed with 2.5% Glutaraldehyde. And all specimen were treated with $OsO_4$, and Tannic acid before drying the cell for coating the cell with gold. Scanning Electron Microscope was used to observation. The following results were obtained. I. During the whole period of experiment, the phenomenon of cell attachment and spreading were revealed similar pattern to compare with smooth surface culture plate and ordinary culture dish. 2. The shape of cell attachment and spreading on the surface of barrier membrane were observed no remarked difference pattern between smooth surface culture plate and ordinary culture dish. 3. The cytoplasmic process of cultured calvaria cell extent to the deep portion of barrier membrane like as their own proper shape. 4. There were no remarkable relationships between the degree of cultured cell spreading and surface structure of barrier membrane. 5. Slight starified layer of cultured calvaria cell were observed on the scattered type of resorbable membrane, Conclusively, this study thus suggest that cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of carrier for many cell which could be used as new tissue regeneration, and those tissue engeering technique may become an new method in the approach to the repair of bone defects.

• 건축역사연구
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• 제21권1호
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• pp.99-118
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• 2012

This study aims to classify the architectural formation of the Stylobate of and Staircase types of Post-Lintel Construction in Backje style and Silla style and also to figure out their specific elements by periods and transformation characteristics. The scope of this study was restricted to architectural remains between the Three Kingdom period and the Goryeo period. To progress the investigation, the study classified remains in a similar type by period and type, and then and analyzed its character based on formation method and specific factors of subsidiary materials. As a result, its type can be classified as Backje type(I), Silla type(II) and Mixed type(III). Regionally, it is found that Woongjin, Sabi and surroundings in the capital of Backje, Gyeongju and main towns in the capital of Silla, and Gaeseong and main towns in the capital of Goryeo. In particular, type III has the characteristics of type I and II simultaneously, which has one or two layers Jangdaeseok stone between the foundation stone of stylobate and plate stone, and tends to be decorative when Taengjuseok stone is installed. For types of staircase, it could be classified as 6~7C Backje type(I), 7~9C Silla type(II) and 9C Mixed type(III) according to the formation method of Somaetdol (banister of stone stair). And from the 9th century on, decorated Somaetdol stone type(IV) of 1/4 circle shape began to appear. The common feature of stylobate of post-lintel and staircase is that it became simplified, abbreviated and decorative. It seems to be intended to secure simplicity of construction or structural stability.

• Restorative Dentistry and Endodontics
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• 제29권4호
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• pp.370-377
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• 2004

The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 ($1{\;}{\times}{\;}10^5{\;}cells/ml/well$) were cultured at 12-well plate at $37^{\circ}C$ for 14 days. Standardized photographs were taken with digital camera during 14 days and the diameter of the contracted collagen gel matrix was measured and statistically analyzed with student t-test. As one of the pulp tissue engineering, normal human dental pulp tissue and collagen gel matrix cultured with dental pulp cells for 14 days were fixed and stained with Hematoxyline & Eosin. According to this study, the results were as follows: 1. The contraction of collagen gel matrix cultured with pulp cells for 14 days was significantly higher than other fibroblasts (gingiva, skin) (p < 0.05), 2. The diameter of collagen gel matrix cultured with pulp cells was reduced to 70.4% after 7 days, and 57.1% after 14 days. 3. The collagen gel without any cells did not contract, whereas the collagen gel cultured with gingiva and skin showed mild contraction after 14 days (88.1% and 87.6% respectively). 4. The contraction of the collagen gel cultured with NIH 3T3 cells after 14 days was higher than those cultured with gingival and skin fibroblasts, but it was not statistically significant (72.1%, p > 0.05). 5. The collagen gel matrix cultured with pulp cells for 14 days showed similar shape with native pulp tissue without blood vessels. This approach may provide a means of engineering a variety of other oral tissue as well and these cell behaviors may provide information needed to establish pulp tissue engineering protocols.

• Structural Engineering and Mechanics
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• 제49권5호
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• pp.569-595
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• 2014

In this paper, a novel semi-energy finite strip method (FSM) is developed based on the concept of first order shear deformation theory (FSDT) in order to attempt the post-buckling solution for thin and relatively thick functionally graded (FG) plates under uniform end-shortening. In order to study the effects of through-the-thickness shear stresses on the post-buckling behavior of FG plates, two previously developed finite strip methods, i.e., semi-energy FSM based on the concept of classical laminated plate theory (CLPT) and a CLPT full-energy FSM, are also implemented. Moreover, the effects of aspect ratio on initial post-buckling stiffness of FG rectangular plates are studied. It has been shown that the variation of the ratio of initial post-buckling stiffness to pre-buckling stiffness ($S^*/S$) with respect to aspects ratios is quite independent of volume fractions of constituents in thin FG plates. It has also been seen that the universal curve representing the variation of ($S^*/S$) with aspect ratio of a FG plate demonstrate a saw shape curve. Moreover, it is revealed that for the thin FG plates in contrast to relatively thick plates, the variations of non-dimensional load versus end-shortening is independent of ceramic-metal volume fraction index. This means that the post-buckling behavior of thin FG plates and the thin pure isotropic plates is similar. The results are discussed in detail and compared with those obtained from finite element method (FEM) of analysis. The study of the results may have a great influence in design of FG plates encountering post-buckling behavior.

• 복합신소재구조학회 논문집
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• 제2권1호
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• pp.15-22
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• 2011

펄트루젼 FRP 구조용 부재는 많은 유용한 역학적, 물리적 성질 때문에 토목분야에서 구조부재의 매력적인 대체부재로 고려될 수 있다. 그러나 펄트루젼 FRP는 탄성계수가 상대적으로 낮고, 부재의 단면이 복부와 플렌지 등의 얇은 판요소로 구성되어 있기 때문에 압축재로 설계할 때 구조적인 안정성은 매우 중요한 고려사항이 된다. 따라서, 압축을 받는 구조용 부재의 설계를 위해, 판요소의 좌굴 및 후좌굴강도를 고려해야 한다. AISC/LRFD의 강구조 설계기준에서는, 후좌굴강도에 추가적인 단면 내 일정하지 않은 응력분포의 영향을 형상계수(form factor)를 사용하여 고려하고 있다. 이 논문에서는 압축력을 받는 펄트루젼 FRP 구조용 부재의 형상계수를 해석적으로 연구하였으며, 형상계수를 설정하는 과정에 대하여 제안하였다.

• 대한조선학회논문집
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• 제28권1호
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• pp.117-124
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• 1991

본 논문에서는 편면 보강판의 압축 강도 해석 시, 유한 요소법의 비경제성을 극복하기 위하여, 붕괴 양식을 가정하고, 각 붕괴 양식에 대해 압축 강도를 구하였다. 최종 강도는 탄성 대변형 해석 곡선과 소성 붕괴를 가정하여 얻은 소성 해석 곡선과의 교점으로 택하였다. 기존의 연구와는 달리 소성 붕괴선의 형상을 변경시켜 최소의 강도 값을 주는 교점을 최종 강도로 택하였다. 최소 강비는 가정한 붕괴 양식의 교점으로 부터 얻어질 수 있다. 탄성 해석에서는 좌굴 파형을 가정하고, 보강재의 비대칭으로 인한 편심 모우멘트를 고려하였으며, 평형 조건식은 Rayleigh-Ritz법을 이용하여 유도하였다. 소성 해석 시에는 고성 붕괴선을 가정하여 소성 붕괴 조건식을 유도하였다. 좌굴과 최종 강도 계산 결과를 유한 요소법에 의한 결과와 비교한 결과, 양호한 일치를 보였다.

• 대한조선학회논문집
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• 제31권1호
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• pp.42-49
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• 1994

조선 CIM 시스템은 제품 모델을 중심으로 한 정보의 통합화를 중점적으로 추구하여야 하므로, 이 제품 모델을 기능 모델과 형상 모델로 구분하였다. 조선을 위한 제품 모델의 근간이 되는 형상 모델을 오일러 조작을 기초로 하는 비다양체 모델의 자료 구조를 이용하여 각각 구획 배치, 구조 설계 및 공정 계획의 입장에서 이용하는 방법에 대하여 연구하였다.

• 한국전산구조공학회논문집
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• 제28권5호
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• pp.459-465
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• 2015

항공기는 목적에 따라서 민간 항공기, 무인항공기, 전투기, 헬리콥터 등 다양한 항공기가 존재한다. 이 각각의 항공기는 특정한 목적에 맞게 형상 및 설계가 된다. 특히 항공기 개발과정에서 중요한 해석과정 중 하나가 구조해석이다. 하지만 항공기 구조가 복잡해지고 3차원 모델로 구조해석을 하게 되면 시간과 비용이 크게 증가하게 된다. 따라서 해석 효율성을 위해서 1차원 등가 보나 2차원 평면 응력 조건을 이용하여 실제 구조를 보다 간단하게 모델링한다. 하지만 이런 모델링은 실제 구조와 차이가 있으므로 실제 구조를 잘 반영할 수 있는 적절한 모델링이 필요하다. 따라서 구조형태에 따라서 1차원 등가 보와 2차원 평면응력 조건을 적절하게 선택하여야 한다. 본 논문에서는 EDISON에 업로드 된 구조해석 프로그램을 이용하여 1차원 구조해석과 2차원 구조해석을 검증하고 구조형태에 따라서 1차원 해석과 2차원 해석을 각각 3차원 MSC NASTRAN 구조해석과 비교하여 적절한 해석방법을 찾고자 한다. 비교결과 길이 대 높이 비가 증가할수록 1차원 해석과 3차원 해석의 오차가 급격히 줄어들었으며 이 비율이 18보다 증가하였을 때는 1차원 해석이 2차원 해석보다 3차원 해석의 결과와 일치하였다.

• 소성∙가공
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• 제27권5호
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• pp.289-295
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• 2018

The profile ring rolling process can realize various ring shapes unlike conventional rectangular cross-sectional ring products. In this paper, the defective groove in the bottom surface of L-shaped ring products was analyzed. Grooves are generated by non-uniform external forces due to profile main roll and initial blank shape. Process parameters such as the motion of dies and working temperature were determined. Mechanism of groove formation was analyzed by FE simulation on the basis of local external forces acting on the blank. Analysis results were similar to the groove actually occurring in the production line. Based on results of the analysis, two solutions were proposed for the groove. The position of the base plate supporting the blank was adjusted and edge length of the main roll was extended to suppress growth of grooves. It has been verified that groove was improved by applying two proposed methods in the shop-floor.

• Tribology and Lubricants
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• 제13권4호
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• pp.60-65
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• 1997

The tungsten disulfide $(WS_2)$ solid lubricant was synthesized by two different reaction processes, i.e., the reaction between $CS_2$ gas phase and solid $WO_3$powder, and the vapour phase transport method of tungsten and sulfur in a high vacuum. The chemical and physical characteristics of synthesized $WS_2$powder were analyzed in terms of the average particle size, morphology, crystalline phase etc. in comparison with those of commercial $WS_2$powder. The solid $WO_3$ powder with the average size of 0.2 ${\mu}{\textrm}{m}$ was reacted with $CS_2$gas flowed with$N_2$or 96%$N_2{\times}4%H_2$forming gas for 36 h and 24 h at 90$0^{\circ}C$ respectively. $WS_2$ crystalline phase was then formed through the intermediate phase of .$W_{20}O_{58}$ In the case of vapour phase transport method, the 3.5 wt% iodine was added as a vapour transport reagent into the composition of tungsten and sulfur powders maintaining a constant molar ratio of W:S=1:2.2. The mixture was then heat treated at 85$0^{\circ}C$ for 2 weeks in vacuum. The reaction product obtained showed the average size of 12 ${\mu}{\textrm}{m}$ and the hexagonal plate shape of typical solid lubricant with 2H-$WS_2$crystalline phase.