• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2929-2937
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• 2015

Endothelial nitric oxide synthase (eNOS or NOS3) produces nitric oxide and genetic polymorphisms of NOS3 gene play significant roles in various processes of carcinogenesis. The results from published studies on the association between NOS3 G894T and NOS3 intron 4 (4a/b) polymorphisms and cancer risk are conflicting and inconclusive. However, i n order to assess this relationship more precisely, a meta-analysis was performed with PubMed (Medline), EMBASE and Google web searches until February 2014 to select all published case-control and cohort studies. Genotype distribution data were collected to calculate the pooled odd ratios (ORs) and 95% confidence intervals (CIs) to evaluate the strength of association. A total of 10,546 cancer cases and 10,550 controls were included from twenty four case-control studies for the NOS3 G894T polymorphism. The results indicated no significant association with cancer risk as observed in allelic (T vs G: OR=1.024, 95%CI=0.954 to 1.099, p=0.508), homozygous (TT vs GG: OR=1.137, 95%CI=0.944 to 1.370, p=0.176), heterozygous (GT vs GG: OR=0.993, 95%CI=0.932 to 1.059, p=0.835), recessive (TT vs GG+GT: OR=1.100, 95%CI=0.936 to 1.293, p=0.249) and dominant (TT+GT vs GG: OR=1.012, 95%CI=0.927 to 1.105, p=0.789) genetic models. Similarly, a total of 3,449 cancer cases and 3,691 controls were recruited from fourteen case-control studies for NOS3 4a/b polymorphism. Pooled results indicated no significant association under allelic (A vs B: OR=0.981, 95%CI=0.725 to 1.329, p=0.902), homozygous (AA vs BB: OR=1.166, 95%CI=0.524 to 2.593, p=0.707), heterozygous (BA vs BB: OR=1.129, 95%CI=0.896 to 1.422, p=0.305), dominant (AA+BA vs BB: OR=1.046, 95%CI=0.779 to 1.405, p=0.763) and recessive (AA vs BB+BA: OR=1.196, 95%CI=0.587 to 2.439, p=0.622) genetic contrast models. This meta-analysis suggests that G894T and 4a/b polymorphisms of NOS3 gene are not associated with increased or decreased risk of overall cancer.

• Journal of Sensor Science and Technology
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• v.27 no.4
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• pp.232-236
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• 2018

A radiation detection sensor was developed and characterized by combining three types of CsI(Tl) scintillators and an array-type SiPM to detect the radioactive contamination of discharged water in real time. The characterization results showed that type 3 exhibited the most desirable characteristics in response linearity (R-square: 0.97889) according to detection sensitivity and incident radiation dose. Furthermore, in terms of spectral characteristics, type 3 exhibited 16.54% at 0.356 MeV (the emission gamma ray energy of $^{133}Ba$), 10.28% at 0.511 MeV (the emission gamma ray energy of $^{22}Na$), 9.68% at 0.356 MeV (the emission gamma ray energy of $^{137}Cs$), and 2.55% and 4.80% at 1.173 MeV and 1.332 MeV (the emission gamma ray energies of $^{60}Co$), respectively. These measurements confirmed the good energy characteristics. The results were used to evaluate the spectral characteristics and energy linearity in a mixed source using type 3 with the best detection characteristics. It was confirmed that the gamma ray peaks of $^{133}Ba$, $^{22}Na$, $^{137}Cs$, and $^{60}Co$ were well resolved. Moreover, it was confirmed that R-square, which is an indicator of energy linearity, was 0.99986. This indicates a good linearity characteristic. Based on this study, further commercialization studies will contribute to measurements in real time and to the management of the contamination caused by radioactive wastewater or radioactive material leakage, which originate from facilities that use radioactive isotopes or care facilities.

• Progress in Superconductivity and Cryogenics
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• v.15 no.4
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• pp.15-20
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• 2013

We investigated the flux pinning properties of both 10 mol% Zr-and Sn-doped $YBa_2Cu_3O_{7-{\delta}}$ (YBCO) films with the same thickness of ~350 nm for a comparative purpose. The films were prepared on the $SrTiO_3$ (STO) single crystal substrate by the metal-organic deposition (MOD) process. Compared with Sn-doped YBCO film, Zr-doped one exhibited a significant enhancement in the critical current density ($J_c$) and pinning force density ($F_p$). The anisotropic $J_{c,min}/J_{c,max}$ ratio in the field-angle dependence of $J_c$ at 77 K for 1 T was also improved from 0.23 for Sn-doped YBCO to 0.39 for Zr-doped YBCO. Thus, the highest magnetic $J_c$ values of 9.0 and $2.9MA/cm^2$ with the maximum $F_p$ ($F_{p,max}$) values of 19 and $5GN/m^3$ at 65 and 77 K for H // c, respectively, could be achieved from Zr-doped YBCO film. The stronger pinning effect in Zr-doped YBCO film is attributable to smaller $BaZrO_3$ (BZO) nanoparticles (the average size ${\approx}28.4$ nm) than $YBa_2SnO_{5.5}$ (YBSO) nanoparticles (the average size ${\approx}45.0$ nm) incorporated in Sn-doped YBCO film since smaller nanoparticles can generate more defects acting as effective flux pinning sites due to larger incoherent interfacial area for the same doping concentration.

• Economic and Environmental Geology
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• v.29 no.3
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• pp.293-304
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• 1996

The Wolchul Mt. area is composed of a biotite granite and a pink feldspar granite. These granites are distinctly different in terms of their field occurrence, mineralogy, trace element and REE composition, as well as their isotope ages. The biotite granite has higher ferromagnesian elements and lower lithophile trace element abundances than the pink feldspar granite. The biotite granite has high Sr and Ba while the pink feldspar granite has high Rb. On the Rb-Sr-Ba diagram the biotite granite plots as a granodiorite while the pink feldspar granite belongs to a strongly differentiated granite. The ${\Sigma}$ LREE/ ${\Sigma}$ REE for the biotite granite is 0.95 and for the pink feldspar granite it is 0.88. The ratio shows a steep decrese in LREE while HREE is essentially constant. Based on the Eu/Sm, $[La/Lu]_{cN}$ and low Eu(-), the biotite granite has quartz diorite to granodiorite composition while the pink feldspar granite, with a relatively high Eu(-) anomaly, falls into the monzo- to syenogranite classification. The silica vs. trace element diagrams for the two granites indicate that the biotite granite could have formed near to a continental margin or volcanic island setting environment while the pink feldspar granite formed within a continental plate or as result of plate collision. The biotite granite has a U-Pb zircon age of 175 Ma, i.e. Middle Jurassic. The pink feldspar granite is younger, it has a K-Ar orthoclase age $93.6{\pm}1.5$ Ma which is Late Cretaceous age.

• Journal of Life Science
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• v.20 no.11
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• pp.1582-1588
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• 2010

A new alginate lyase gene of marine bacterium Streptomyces sp. M3 had been previously cloned in pColdI vector and transformed into E. coli BL21 (DE3). In this study, M3 lyase protein without signal peptide was overexpressed by induction with IPTG and purified with Ni-Sepharose affinity chromatography. The absorbance at 235 nm of the reaction mixture and TLC analysis showed that M3 alginate lyase was a polyG-specific lyase. When M3 lyase was assayed with substrate for 10 min, optimum pH and optimum temperature were pH 9 and $60^{\circ}C$. For the effect of 1mM metal ion on M3 lyase activity, $Ca^{++}$ and $Mn^{++}$ ions increased the alginate degrading activity by two-fold, whereas $Hg^{++}$ and $Zn^{++}$ ions inhibited the lyase activity completely. $Mg^{++}$, $Co^{++}$, $Na^+$, $K^+$, and $Ba^{++}$ did not show any strong effects on alginate lyase activity.

• Journal of Life Science
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• v.9 no.4
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• pp.414-422
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• 1999

Our works performed for preparation of oligosaccharides from carrageenan, seaweed polysaccharide, and one active strain for carrageenan was isolated from sea water and identified to Pseudomonas alcaligenes. Carrageenan degrading enzyme was purified from the culture fluid of isolated strain-Pseudomonas alcaligenes JCL-43, by DEAE-Cellulose, Sephadex G-100, Q-Sepharose and CM Sepharose CL-6B column chromatography. Two enzyme-F-I, F-II- was identified this purifying process, and the molecular weight of the purified carrageenase were estimated to be 23.6kDa and 30.2kDa, respectively. The optimum pH and temperature for two carrageenase activity were 7.0 and 4$0^{\circ}C$. These enzymes were stable in the pH range of 6.0~7.5 and lower than 5$0^{\circ}C$, and required 1.5% NaCl for optimum activity. And these carragennase were inhibited by metal ions such as Cu2+, Zn2+, Hg2+, but increased by Ba2+ and Ca2+, and showed specificity on -carrageenan.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.97-100
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• 2015

The thermostable pectinase gene TmPec isolated from Thermotoga maritima was introduced into the NdeI site of pRSET-B vector and expressed in its intact form in Escherichia coli BL21. The overexpressed intact form of pectinase (TmPecN protein) was partially purified by heat-denaturation procedure. TmPecN showed the highest activity between 85 and $95^{\circ}C$, and at approximately pH 6.5. Enzyme activity was stably maintained at temperatures below $85^{\circ}C$. In the presence of $Ca^{2+}$, pectinase activity of TmPecN increased to 128.4% of normal level. In contrast, $Ba^{2+}$, $Zn^{2+}$, and $Mn^{2+}$ strongly inhibited TmPecN activity. We conclude that the biochemical properties of the intact form of TmPecN are comparable to those of the recombinant protein TmPec reported previously.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.4
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• pp.185-195
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• 2020

The study aims to assess the impact of destination image, satisfaction and loyalty of tourists at mountain destinations in Thanh Hoa province, Vietnam. The study involves questionnaire surveys and multivariate data analysis methods (Cronbach Alpha test, EFA, CFA, SEM). Research results from 500 tourists in the mountain destinations of Thanh Hoa province demonstrate that all factors have imposed a positive impact on tourist satisfaction, specifically: The most influential factor is Natural features, followed by Human factors while the least influential factor is Infrastructure; On the other hand, research results also demonstrate that satisfaction has a substantial impact on tourist loyalty. Based on the research results, we also proposed some key solutions to enhance the destination image, thereby contributing to increased satisfaction and loyalty of tourists, including: (i) Promoting Natural Tourism Resources. (ii) Raising Awareness of Environmental Protection. (iii) Building Local Cultural Identity. (iv) Building Exclusive Tourist Products. (5) Strengthening the Support of Local Authorities for Tourism Activities. (vi) Developing a Price Policy.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.245-252
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• 2006

A bacterial strain, identified as Staphylococcus aureus JJ-11, producing collagenase was isolated out of 40 persons having skin troubles. S. aureus JJ-11 produced collagenase optimally in the media containing 1.5%(w/v) gelatin, 1%(w/v) yeast extract, 0.4%(w/v) $K_2HPO_4$, 0.005%(w/v) $NiSO_4{\cdot}6H_2O$ at $37^{\circ}C$ for 18 hrs. The collagenase produced by Staphylococcus aureus JJ-11 was purified at 6.66-folds purity through application of chromatography with Amberlite IRA-900 and Sephacryl S-300 HR columns. The molecular weight of the partially purified enzyme was estimated to be 62 kDa by SDS-PAGE. The protein exhibited optimum enzymatic activity at pH 7.0, and showed a stable activity at pH 4-8. The optimum temperature for collagenase was at $37^{\circ}C$, and activity was maintained upto $40^{\circ}C$. The enzyme activity was slightly elevated in the presence of divalents such as, $Fe^{2+},\;Co^{2+}\;and\;Ba^{2+}$ However, the activity was inhibited in the presence of $Sr^{2+}\;or\;Hg^{2+}$. The inhibition of activity by O-phenanthroline and EDTA suggested that the enzyme may contain metal which is required for activity. The enzyme showed the highest activity when insoluble collagen (type I) was, used as a substrate.

• Analytical Science and Technology
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• v.10 no.3
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• pp.161-167
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• 1997

This is on hydrogen ion-selective memebrane electrodes which were made of tetrabenzylmethylenediamine (TBMDA), tetrabenzylethylenediamine (TBEDA), tetrabenzylpropylenediamine(TBPDA) and tetrabenzylhexylenediamine(TBHDA) as neutral carriers. Their response potentials to carbon number between amino groups showed linear selectivities to hydrogen ion in the range of pH 1~pH 9, pH 2~pH 9, pH 3~pH 9 and pH 4~pH 9 and slopes were 48mV/pH, 52mV/pH, 64mV/pH, 59mV/pH respectively. The interferences effect on the cations were measured to alkali metal ions($Li^+$, $Na^+$, $K^+$), alkaline earth metal ions ($Mg^{2+}$, $Ca^{2+}$, $Sr^{2+}$, $Ba^{2+}$), transition metals ions($Cu^{2+}$, $Ni^{2+}$, $Co^{2+}$) and anions($I^-$, $Br^-$, ${NO_3}^-$, $SCN^-$), and selectivity coefficients were measured by separate-solution method. The membrane electrode made of TBMDA among the electrodes showed the best selectivity in acidic solution.