• Title/Summary/Keyword: I-섬유

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The Effect of the IGF-I treated Gingival and Periodontal Ligament Fibroblast on Osteoblasts (IGF-I으로 처리한 치은 및 치주인대 섬유모세포가 골모세포에 미치는 영향)

  • Kim, Mi-Jeong;Yang, Won-Sik
    • The korean journal of orthodontics
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    • v.31 no.6 s.89
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    • pp.589-600
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    • 2001

  • Insulin-like growth factor I (IGF-I) has the local tissue regulating actions. In bone, IGF-I increases the replication of osteoblastic lineage, probably preosteoblasts, and enhances osteoblastic collagen synthesis and matrix composition rates. The purpose of this study was to investigate the local regulatory effect of IGF-I on periodontium totally, both in an autocrine and paracrine manner. To examine the effect of IGF-I directly on osteoblast (OB) of test rats, and indirectlv on OB via periodontal ligament fibroblast (PDLF), and the effect of gingival fibroblast (GF) on OB via cellular paracrine manner for the understanding of humoral action of adjacent tissue, GF and PDLF were obtained from male Sprague-Dawley rats of six to eight weeks of age. OB was obtained iron frontal and parietal calvarial bone of Sprague-Dawley 21day-old-fetus. After each tell was Incubated 24 hours, for collecting conditioned medium, different concentrations of IGF-I (1,10,100 ng/ml,1ml/well) was adding in the GF, PDLF cells, and the supernatant from these cultures was put into the primary OB culture with $1{\times}10^4$cell/ml/well. The experimental group was divided into six groups control OB, IGF-I treated OB, OB culture with conditioned medium from PDLF, OB culture with conditioned medium from IGF-I treated PDLF, OB culture with conditioned medium from GF, OB culture with conditioned medium from IGF-I treated GF. After final IGF-I treatment, OB was Incubated for 24 hours, and alkaline phosphatase activity assay, BMP expression, cell proliferation measurement using MTT assay, total protein measurement, Collagen synthesis assay using western blot, and examination of bone nodule synthesis were done. Alkaline phosphatase expressions were increased in the group of PDLF-IGF-I supernatant treatment. Direct IGF-I treatment with concentrations of 100ng/m1 showed increased viable tell number measured by MTT assay. And IGF-I treatment did not increase total protein amount. The entire experimental group showed BMP2, 4 expression in western blot, and there was no significant difference between control and experimental groups. These results suggested that supernatant from PDLF effects on increasing cellular activities of OB regardless of IGF-I, and at high concentration, IGF-I increases OB tell proliferation.

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Characteristics of Carbon Fiber Composites for the Application of Smart Sensor (I) (스마트 센서로의 적용을 위한 탄소섬유 복합체의 특성평가 (I))

  • 김유택
    • Journal of the Korean Ceramic Society
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    • v.38 no.1
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    • pp.52-55
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    • 2001

  • Polyacrylonitrile(PAN)-based 탄소섬유와 epoxy resin matrix 내에 장착된 PAN-based 탄소섬유의 전기저항을 탄소섬유와 파괴될 때까지 인장강도를 증가시키며 측정하였다. 탄소섬유가 끊어져 측정 계기가 open-circuit을 나타내기 전까지 탄소섬유의 전기저항 값은 strain이 증가할수록 증가하였으며, epoxy resin은 탄소섬유의 저항값 측정에 거의 영향을 미치지 않았다. 탄소섬유의 저항값 변화는 strain에 대해 정확도 1% 이내로 예측(calibrate)할 수 있었으며 재현성 또한 우수하였다. 따라서 탄소섬유는 재료의 기계적 강도를 증가시키기 위해 사용될 수 있을 뿐만 아니라 구조재료의 파괴를 예측할 수 있는 스마트 센서로서도 사용할 수 있다는 가능성을 확인하였다.

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Wet Spinning of Silk Polymer(I) -Morphological characteristics of regenerated silk fibroin filament- (실크 고분자의 습식방사(I) -재생 실크 필라멘트의 형태학적 구조 특성-)

  • 엄인철;권해용;박영환;임대우
    • Proceedings of the Korean Fiber Society Conference
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    • 2001.10a
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    • pp.123-126
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    • 2001

  • 실크는 우수한 광택, 촉감, 물성을 바탕으로 하여 인류에 있어 최고의 의류용 섬유로서 널리 이용되어 왔으며 최근에는 생체적합성 등을 활용한 의료용 및 생물공학용 소재로서 응용하고자 하는 연구가 활발히 진행 중에 있다[1-2]. 그러나 실크의 경우 누에로부터 얻어지는 천연섬유로서 합성섬유와는 달리 누에로부터 토사되면서 그 구조 및 형태가 결정되어 고정되는 단점을 안고 있어 의류용 및 산업용 분야로의 활용에 있어 한계가 있다. (중략)

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