• Title/Summary/Keyword: I/S and I/O Transformation

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Molecular Cloning of Bacillus stearothermophilus cdd Gene Encoding Thermostable Cytidine/Deoxycytidine Deaminase (Bacillus stearothermophilus 의 내열성 시티딘/디옥시시티딘 디아미나제를 코드하는 cdd 유전자의 클로닝)

  • Soo, Chang-Jong;Song, Bang-Ho;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.17 no.4
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    • pp.334-342
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    • 1989
  • The Bacillus stearothermophilus cdd gene encoding cytidine deaminase (cytidine/2'-deoxycytidine aminohydrolase; EC 3.5.4.5) was isolated through shot gun cloning by oomplementation of an E. coli cdd mutation. Primarily 3.0 kbp of the exogenote was cloned into the Pstl site of pBR322 (pJSC101). By subsequent deletion and subcloning from the insert of pJSC101 with cdd$^+$ and tetracycline resistancy, about 1.35 kbp of the EcoRI$_1$/PstI$_2$ fragment containing the cdd gene was isolated as pJSC201. The minicell experiment revealed a molecular mass of 33,000 dalton for polypeptide from the cloned DNA fragment complementing the cdd gene. From the lacZ fusion of 550 bp fragment of the EcoRI$_1$/AuaI as a putative promoter region, the transcription direction of the cdd gene on pJSC201 is from EcoRI towards the PstI sites, When the cdd gene was expressed in B. subtilis ED4O (cdd$^-$, pyr$^-$) by transformation with the E. coli-B. subtilis shuttle vector, the gene expression occured more efficiently than in E. coli and the gene appears to be stably maintained in B. subtitis as well as in E. coli.

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A Destructive Method in the Connection of the Algorithm and Design in the Digital media - Centered on the Rapid Prototyping Systems of Product Design - (디지털미디어 환경(環境)에서 디자인 특성(特性)에 관한 연구(硏究) - 실내제품(室內製品) 디자인을 중심으로 -)

  • Kim Seok-Hwa
    • Journal of Science of Art and Design
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    • v.5
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    • pp.87-129
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    • 2003
  • The purpose of this thesis is to propose a new concept of design of the 21st century, on the basis of the study on the general signification of the structures and the signs of industrial product design, by examining the difference between modern and post-modern design, which is expected to lead the users to different design practice and interpretation of it. The starting point of this study is the different styles and patterns of 'Gestalt' in the post-modern design of the late 20th century from modern design - the factor of determination in industrial product design. That is to say, unlike functional and rational styles of modern product design, the late 20th century is based upon the pluralism characterized by complexity, synthetic and decorativeness. So far, most of the previous studies on design seem to have excluded visual aspects and usability, focused only on effective communication of design phenomena. These partial studies on design, blinded by phenomenal aspects, have resulted in failure to discover a principle of fundamental system. However, design varies according to the times; and the transformation of design is reflected in Design Pragnanz to constitute a new text of design. Therefore, it can be argued that Design Pragnanz serves as an essential factor under influence of the significance of text. In this thesis, therefore, I delve into analysis of the 20th century product design, in the light of Gestalt theory and Design Pragnanz, which have been functioning as the principle of the past design. For this study, I attempted to discover the fundamental elements in modern and post-modern designs, and to examine the formal structure of product design, the users' aesthetic preference and its semantics, from the integrative viewpoint. Also, with reference to history and theory of design my emphasis is more on fundamental visual phenomena than on structural analysis or process of visualization in product design, in order to examine the formal properties of modern and post-modern designs. Firstly, In Chapter 1, 'Issues and Background of the Study', I investigated the Gestalt theory and Design Pragnanz, on the premise of formal distinction between modern and post-modern designs. These theories are founded upon the discussion on visual perception of Gestalt in Germany in 1910's, in pursuit of the principle of perception centered around visual perception of human beings. In Chapter 2, I dealt with functionalism of modern design, as an advance preparation for the further study on the product design of the late 20th century. First of all, in Chapter 2-1, I examined the tendency of modern design focused on functionalism, which can be exemplified by the famous statement 'Form follows function'. Excluding all unessential elements in design - for example, decoration, this tendency has attained the position of the international style based on the spirit of Bauhause - universality and regularity - in search of geometric order, standardization and rationalization. In Chapter 2-2, I investigated the anthropological viewpoint that modern design started representing culture in a symbolic way including overall aspects of the society - politics, economics and ethics, and its criticism on functionalist design that aesthetic value is missing in exchange of excessive simplicity in style. Moreover, I examined the pluralist phenomena in post-modern design such as kitsch, eclecticism, reactionism, hi-tech and digital design, breaking away from functionalist purism of modern design. In Chapter 3, I analyzed Gestalt Pragnanz in design in a practical way, against the background of design trends. To begin with, I selected mass product design among those for the 20th century products as a target of analysis, highlighting representative styles in each category of the products. For this analysis, I adopted the theory of J. M Lehnhardt, who gradated in percentage the aesthetic and semantic levels of Pragnantz in design expression, and that of J. K. Grutter, who expressed it in a formula of M = O : C. I also employed eight units of dichotomies, according to the G. D. Birkhoff's aesthetic criteria, for the purpose of scientific classification of the degree of order and complexity in design; and I analyzed phenomenal aspects of design form represented in each unit. For Chapter 4, I executed a questionnaire about semiological phenomena of Design Pragnanz with 28 units of antonymous adjectives, based upon the research in the previous chapter. Then, I analyzed the process of signification of Design Pragnanz, founded on this research. Furthermore, the interpretation of the analysis served as an explanation to preference, through systematic analysis of Gestalt and Design Pragnanz in product design of the late 20th century. In Chapter 5, I determined the position of Design Pragnanz by integrating the analyses of Gestalt and Pragnanz in modern and post-modern designs In this process, 1 revealed the difference of each Design Pragnanz in formal respect, in order to suggest a vision of the future as a result, which will provide systemic and structural stimulation to current design.

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The Study about Style-Up Design Development as a Motive of Organic Line in Art Nouveau Style (아르누보 양식의 유기적인 선을 모티브로 한 업스타일 디자인 개발에 관한 연구)

  • Lee, Jin-Hee;An, Jong-Suk
    • Journal of the Korean Society of Costume
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    • v.62 no.2
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    • pp.68-77
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    • 2012
  • Hair style takes a large part in emphasizing to the appearance and the effect of image transformation with fashion to the modern people whose competition is appearance in 21 century. According to the development of mass media and the exchange of the life style. Image creation which correspond to T.P.O is needed and style-up is also required in modern costume, as the coordination for special day is routinized. In this study, the expression of a fine and organic line shown as a motive in Art Nouveau style's works is applied to style-up design and a new view sight to the style-up design through the art history is suggested with developing style-up design to satisfy the upgraded customer's requirements. The feminine beauty is appeared as a various image by applying style-up design from Art Nouveau style through this study. and the infinite possibility to the design development can be found with activated motive of Art Nouveau. These Art Nouveau trend will be a foundation to the inspiration of high artistic design and will be helpful to the aesthetic satisfaction of the elegance modern people. Therefore, I hope this study will be a fundamental data for creative development of hair design through the art history which may be forgetting.

Preparation and Characterization of Half-Sandwich Cobalt(III) Complexes of Cp Ligands with a Rigid Thioanisole Side-Chain

  • S, Sujith;Lee, Bun-Yeoul;Han, Jin-Wook
    • Bulletin of the Korean Chemical Society
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    • v.28 no.8
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    • pp.1299-1304
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    • 2007
  • New sulfur functionalized cyclopentadiene ligands, 1-[2-(thioanisole)]-2,5-dimethylcyclopentadiene (3), 1-[2- (thioanisole)]-2,3,5-trimethylcyclopentadiene (4), and 1-[2-(thioanisole)]-2,3,4,5-tetramethylcyclopentadiene (5), were prepared. In these ligands, the S-donor atom is connected to a cyclopentadiene ring by a rigid phenylene spacer. CpCo(III)-diiodo half-sandwich complexes (6-8) were obtained from reaction the ligands (3- 5) with Co2(CO)8, followed by treatment of I2. Substitution reaction of CpCo(III)-diiodo complexes with MeLi yielded the corresponding CpCo(III)-dimethyl complexes (9-11). Further transformation to the corresponding cationic cobalt complexes (12-14) were achieved by reaction of the CpCo(III)-dimethyl complexes with HB(ArF)4·2Et2O and trapping with CD3CN. The new sulfur functionalized cyclopentadiene ligands having a rigid phenylene spacer and the corresponding cobalt complexes were characterized by 1H, 13C and 19F NMR spectroscopy. The diiodo Complex 6 was also characterized by a single crystal X-ray diffraction method.

CHARACTERISTICS OF HETEROEPITAXIALLY GROWN $Y_2$O$_3$ FILMS BY r-ICB FOR VLSI

  • Choi, S.C.;Cho, M.H.;Whangbo, S.W.;Kim, M.S.;Whang, C.N.;Kang, S.B.;Lee, S.I.;Lee, M.Y.
    • Journal of the Korean institute of surface engineering
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    • v.29 no.6
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    • pp.809-815
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    • 1996
  • $Y_2O_3$-based metal-insulator-semiconductor (MIS) structure on p-Si(100) has been studied. Films were prepared by UHV reactive ionized cluster beam deposition (r-ICBD) system. The base pressure of the system was about $1 \times 10^{-9}$ -9/ Torr and the process pressure $2 \times 10^{-5}$ Torr in oxygen ambience. Glancing X-ray diffraction(GXRD) and in-situ reflection high energy electron diffracton(RHEED) analyses were performed to investigate the crystallinity of the films. The results show phase change from amorphous state to crystalline one with increasingqr acceleration voltage and substrate temperature. It is also found that the phase transformation from $Y_2O_3$(111)//Si(100) to $Y_2O_3$(110)//Si(100) in growing directions takes place between $500^{\circ}C$ and $700^{\circ}C$. Especially as acceleration voltage is increased, preferentially oriented crystallinity was increased. Finally under the condition of above substrate temperature $700^{\circ}C$ and acceleration voltage 5kV, the $Y_2O_3$films are found to be grown epitaxially in direction of $Y_2O_3$(1l0)//Si(100) by observation of transmission electron microscope(TEM). Capacitance-voltage and current-voltage measurements were conducted to characterize Al/$Y_2O_3$/Si MIS structure with varying acceleration voltage and substrate temperature. Deposited $Y_2O_3$ films of thickness of nearly 300$\AA$ show that the breakdown field increases to 7~8MV /cm at the same conditon of epitaxial growing. These results also coincide with XPS spectra which indicate better stoichiometric characteristic in the condition of better crystalline one. After oxidation the breakdown field increases to 13MV /cm because the MIS structure contains interface silicon oxide of about 30$\AA$. In this case the dielectric constant of only $Y_2O_3$ layer is found to be $\in$15.6. These results have demonstrated the potential of using yttrium oxide for future VLSI/ULSI gate insulator applications.

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Functional Brain Mapping Using $H_2^{15}O$ Positron Emission Tomography ( I ): Statistical Parametric Mapping Method ($H_2^{15}O$ 양전자단층촬영술을 이용한 뇌기능 지도 작성(I): 통계적 파라메터 지도작성법)

  • Lee, Dong-Soo;Lee, Jae-Sung;Kim, Kyeong-Min;Chung, June-Key;Lee, Myung-Chul
    • The Korean Journal of Nuclear Medicine
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    • v.32 no.3
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    • pp.225-237
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    • 1998
  • Purpose: We investigated the statistical methods to compose the functional brain map of human working memory and the principal factors that have an effect on the methods for localization. Materials and Methods: Repeated PET scans with successive four tasks, which consist of one control and three different activation tasks, were performed on six right-handed normal volunteers for 2 minutes after bolus injections of 925 MBq $H_2^{15}O$ at the intervals of 30 minutes. Image data were analyzed using SPM96 (Statistical Parametric Mapping) implemented with Matlab (Mathworks Inc., U.S.A.). Images from the same subject were spatially registered and were normalized using linear and nonlinear transformation methods. Significant difference between control and each activation state was estimated at every voxel based on the general linear model. Differences of global counts were removed using analysis of covariance (ANCOVA) with global activity as covariate. Using the mean and variance for each condition which was adjusted using ANCOVA, t-statistics was performed on every voxel To interpret the results more easily, t-values were transformed to the standard Gaussian distribution (Z-score). Results: All the subjects carried out the activation and control tests successfully. Average rate of correct answers was 95%. The numbers of activated blobs were 4 for verbal memory I, 9 for verbal memory II, 9 for visual memory, and 6 for conjunctive activation of these three tasks. The verbal working memory activates predominantly left-sided structures, and the visual memory activates the right hemisphere. Conclusion: We conclude that rCBF PET imaging and statistical parametric mapping method were useful in the localization of the brain regions for verbal and visual working memory.

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The Role of H2O as PCA and the Precipitation Behavior in Mechanically Alloyed Ni-20Cr-20Fe-5Nb Alloy (기계적합금화로 제조된 Ni-20Cr-20Fe-5Nb 합금에서 PCA로서 H2O의 역할과 시효석출거동)

  • Kim Il-Ho;Kwun S.I.;Lee Won-Sik;Chae S.W.;Hwang S.K.;Kim M.H.
    • Journal of Powder Materials
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    • v.13 no.3 s.56
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    • pp.178-186
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    • 2006
  • The effect of use of $H_2O$ as PCA(process control agent) to prevent the carbon contamination during mechanical alloying process and the precipitation behavior in Ni-20Cr-20Fe-5Nb bulk alloy after aging were investigated. NbC and $Cr_2O_3$ were formed during mechanical alloying and consolidation processes in the Ni-20Cr-20Fe-5Nb alloy in which methanol($CH_3OH$) was added as PCA. Formation of NbC in this alloy decreased the amount of Nb dissolved in the Ni matrix. The use of $H_2O$ as PCA in Ni-20Cr-20Fe-5Nb alloy prevented the formation of NbC and increased the hardness. The increase of hardness in this alloy was attributed to the increased amount of Nb dissolved in the Ni matrix. After aging treatment for 20 hours at $600^{\circ}C\;and\;720^{\circ}C$ of Ni-20Cr-20Fe-5Nb bulk alloy in which $H_2O$ added as PCA, ${\gamma}"$$(Ni_3Nb,\;tetragonal)\;and\;{\delta}\;(Ni_3Nb,\;orthorhombic)$ precipitates were formed, respectively. The precipitation temperatures of ${\gamma}"$ and ${\delta}$ in this bulk alloy were lower than those in commercial IN 718 alloy. It seemed that the lower precipitation temperatures for ${\gamma}"$ and ${\delta}$ in this bulk alloy than in commercial IN 718 alloy were due to severe plastic deformation during mechanical alloying.

Physiology and Growth of Transgenic Tobacco Plants Containing Bacillus subtilis Protoporphyrinogen Oxidase Gene in Response to Oxyfluorfen Treatment (Bacillus subtilis Protoporphyrinogen Oxidase 유전자 형질전환 담배의 Oxyfluorfen 처리에 대한 생리 · 생장반응)

  • Lee, J.J.;Kuk, Y.I.;Chung, J.S.;Lee, S.B.;Choi, K.W.;Han, O.S.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.237-245
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    • 1998
  • The transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants containing Bacillus subtilis protoporphyrinogen oxidase gene with cauliflower mosaic virus 35S promoter have recently been generated by using Agrobacterium-mediated gene transformation. The nontransgenic and the transgenic tobacco plants were compared with respect to responses to diphenyl ether herbicide oxyfluorfen and under various environmental conditions. Both cellular leakage and lipid peroxidation caused by oxyfluorfen were found to be less in the transgenic than in the nontransgenic plants. Growth responses of the transgenic plants under various temperature, light, and water conditions were almost the same as those of the nontransgenic plants, although the transgenic plants exhibited slightly more retarded growth under low light or saturated water condition. These results revealed that the transgenic tobacco plants containing B. subtilis protoporphyrinogen oxidase gene under cauliflower mosaic virus 35S promoter were relatively resistant to oxyfluorfen and exhibited normal growth pattern. Possible mechanism of resistance to oxyfluorfen in the transgenic plants is also discussed.

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Study on the bolt-leaf-hanges in Three-yum and Three yang based on Treatise on the Darting and meeting of Yum and yang (음양이합론(陰陽離合論)을 바탕으로한 개합추(開闔樞) 이론(理論) 연구(硏究))

  • Ha, Keun-Ho;Kwon, Young-Kyu;Kim, Kwang-Joong
    • Journal of Oriental Physiology
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    • v.14 no.2 s.20
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    • pp.157-178
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    • 1999
  • In terms of the bolt-leaf-hanges in Three-yum and Three yang I referd to successive medical books and many recent literatures on the basis of Huangjenegeong Somun Treatise on the Darting and meeting of Yin and yang, thus considerd concept of successive commentators about the bolt-leaf-hanges in Three-yum and Three yang and reconstructed and systeamtically arranged the bolt-leaf-hanges's interrelationship, yum-yang's relation and movement of six chnnel, and then studied there trinciple in all the universe apply the body and the process is operated by the bolt-leaf-hanges in the trinciples followed in human make his life adaption environment. The process is operated by the bolt-leaf-hanges in Three-yum and Three yang. The trinciples followed in. Firstly, Three-yum and Three yang is not dirrerentiate by many, middle, little of yum-yanggi's different function & role. Secondly, Three-yum and Three yang is negatively related white showing that there is the bolt-leaf-hanges relationship through space of Three-yum and Three yang. Through internal relationship of Three yum channel & Three yang channel, it can be proven that Three-yum and Three yang is in negative relation and through achieving the bolt-leaf-hanges relation in dimensional concept, it can be proven that it can actually be applied to human. Thirdly, by showing Three-yum and Three yang's the bolt-leaf-hanges relation is relation between Three yum & Three yang as well as negative relation, it can be shown that Three-yum and Three yang's the bolt-leaf-hanges activity is circular, and that it maintains itself through appropriate transformation. Fourth, understanding of Three-yum and Three yang's the bolt-leaf-hanges relationship and yum-yang Concern & six channer's activity has been varied. It means that the understanding is affected by socity, philosophical & research culture of the various times. The purpose has been to systematically arrange and diagnosing Three-yum and Three yang's the bolt-leaf-hanges and thus make up a fundamental for recognition. But, further study should be carried out on medical explanation on the bolt-leaf-hanges and interpretation and operation of the bolt-leaf-hanges in O-UnYukKiron.

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Study on the Inheritance and Transformation of the Goryeo Tradition in the Illustrations of the Sixteen Contemplations of the Amitayurdhyana Sutra of the Early Joseon Dynasty -with the Focus on the Concept of Rebirth in the Pure Land- (조선전기 관경십육관변상도에 보이는 고려 전통의 계승과 변용 -정토인식과 왕생관의 변화를 통해-)

  • Lee, Seung-Hee
    • Korean Journal of Heritage: History & Science
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    • v.51 no.1
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    • pp.126-147
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    • 2018
  • The compositions and iconographies of the three Illustrations of the Sixteen Contemplations produced in the early Joseon Dynasty effectively manifest the transitional period of Joseon society as well as the Joseon people's perception of the Pure Land. In particular, the painting at titled Chionji (知恩寺) dated 1435 and another painting at titled Chion' in (知恩院) from 1465, which were commissioned by the Joseon royal family and high-ranking monk, embody the Pure Land faith of the Tiantai School of Buddhism (天台宗) which thrived during the late Goryeo period. This indicates that the Joseon royal court continued to favor the Pure Land faith that emphasized salvation through "self-power" (自力), which was popular among both the royal family and the nobility of Goryeo. On the other hand, in the painting at titled $H{\bar{o}}rinji$ (法輪寺), which is presumed to have been made in the sixteenth century, the compositions and iconographies shown in late Goryeo examples of the Illustrations of the Sixteen Contemplations are disassembled. Moreover, a new iconographical motif, i.e. "welcoming the dragon boat" (龍船接引), emerged with this painting. This motif symbolizes the idea that the souls of the deceased are guided by Amitabha Buddha to rebirth in the Pure Land of Extreme Bliss, which is based on the "other-power" of the Pure Land faith that emphasizes the power of Amitabha Buddha in attaining salvation rather than the educational "self-power" of the Pure Land faith. The emergence of this motif demonstrates the transformation of the Pure Land belief into a faith that relied on the "other-power" of Amitabha in Joseon's Confucian society.