• Biomedical Science Letters
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• v.4 no.1
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• pp.43-56
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• 1998

A multiplex PCR method was designed by employing primers specific for the eaeA gene, conserved sequences of Shiga-like toxins (SLT-I.II), and the 60-MDa plasmid of enterohemorrhagic E. coli (EHEC) O157:H7 strain. A set of six synthetic oligonucleotide primers derived from sequences of the SLT-I.II, eaeA, and 60-MDa plasmid genes of E. coli O157:H7 were used in a multiplex PCR amplification procedure to detect these genes in the same enteric pathogens. In two enterohemorrhagic E. coli O157:H7 (ATCC 35150, ATCC 43894) reference strains, PCR products of 317bps (eaeA), 228bps (SLT-I.II), and 167bps (60-MDa plasmid) were successfully amplified simultaneously in a single reaction. However, the specific PCR products were not amplified in control strains of other enteric bacteria. The sensitivity of the multiplex PCR assay for detection of the SLT-I.II, eaeA, and 60-MDa plasmid genes of E. coli O157:H7 was found to be 2.5$\times$10$^{6}$ of bacteria in diarrheal stool to amplify all three bands. The multiplex PCR technology will allow large-scale screening of many clinical specimens or contaminated foods, and will be a very useful method for the detection of a wide range of microorganisms present in the environment, including EHEC O157:H7 in various types of specimens. The multiplex PCR assay has the potential to be used as a specific and rapid method for clinical diagnosis of disease caused by EHEC O157:H7.

• Journal of Intelligence and Information Systems
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• v.18 no.1
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• pp.91-107
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• 2012

As the Internet becomes more popular, many people use it to communicate. With the increasing number of personal homepages, blogs, and social network services, people often expose their personal information online. Although the necessity of those services cannot be denied, we should be concerned about the negative aspects such as personal information leakage. Because it is impossible to review all of the past records posted by all of the people, an automatic personal information detection method is strongly required. This study proposes a method to detect or classify online documents that contain personal information by analyzing features that are common to personal information related documents and learning that information based on the Na$\ddot{i}$ve Bayes algorithm. To select the document classification algorithm, the Na$\ddot{i}$ve Bayes classification algorithm was compared with the Vector Space classification algorithm. The result showed that Na$\ddot{i}$ve Bayes reveals more excellent precision, recall, F-measure, and accuracy than Vector Space does. However, the measurement level of the Na$\ddot{i}$ve Bayes classification algorithm is still insufficient to apply to the real world. Lewis, a learning algorithm researcher, states that it is important to improve the quality of category features while applying learning algorithms to some specific domain. He proposes a way to incrementally add features that are dependent on related documents and in a step-wise manner. In another experiment, the algorithm learns the additional dependent features thereby reducing the noise of the features. As a result, the latter experiment shows better performance in terms of measurement than the former experiment does.

• Journal of the Korean Society for information Management
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• v.26 no.3
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• pp.279-294
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• 2009

This research evaluated Information Application Mechanism based on university student's preference. In other words, the research content is analyzing what is the most effective learning method for lectures. To obtain this research objective I've done some research in theoretical research and pervious research related to informational mechanism. I also analyzed the degree of understanding in learning-efficiency with students based on lectures using various information mechanisms. For the data gathering I've surveyed 82 students living in Busan, and used the survey sheets to gather data, I made the survey sheet with nominal and interval parts to make the analyzement of data easier. The research result is down below. First, It shows that the students with higher understanding in IT preferred lectures with "PPT"s. Second, The "PPT" preference group thought that the "PPT" teaching method is more effective than other methods. Third, The "WRT" preference group thought that the "WRT" teaching method is more effective than other methods. Forth, Both groups gave each method a 3.08 and 2.95 as a total mean value out on a 1-5 scale showing not much difference. However this research has a research limitation like other researches. In other words, there can be some distortion of information when using the interview method to gather data. The researches from now on will need to organize groups by information mechanisms and research the learning-efficiency with each mechanism.

• The Journal of the Acoustical Society of Korea
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• v.42 no.6
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• pp.565-571
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• 2023

With the introduction of the post-verification system, the measurement of floor impact noise performance on-site has become mandatory, and the evaluation method has changed. To track the performance changes since the policy implementation, research is needed on how the characteristics of heavyweight impact sound change according to the varied evaluation method. In this study, we analyzed the contribution rate of the frequency band-specific sound pressure level on the single-number quantity for a multi-family housing unit with the same floor plan and floor structure, comprising 59 households, based on the changed impact sources and evaluation indicators. It is difficult to compare simply because the method of calculating contributions by frequency band according to the single-day evaluation is different, but the average contribution rate of 63 Hz was 80.8 % in the evaluation method before the introduction of the post-confirmation system (Tire measurement and evaluated as L'_i,Fmax,AW), and the average contribution rate of 125 Hz was 19.2 %. The current evaluation method (rubber ball measurement and evaluation as L'_iA,Fmax) shows that the contribution rate has decreased to 33.1 % on average at 50 Hz ~ 80 Hz, 58.7 % on average at 100 Hz ~ 160 Hz, 6.9 % on average at 200 Hz ~ 315 Hz, and 1.3 % on average at 400 Hz ~ 630 Hz. This result is a case analysis for the target apartment house, and it is necessary to analyze measurement data for more diverse apartment houses.

• Journal of Korea Technology Innovation Society
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• v.17 no.2
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• pp.334-354
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• 2014

This study aims to analyze the research trend of Korean medicine based on social network analysis. To do this, a dataset has been collected from KCI (Korea Citation Index) database. According to the results, we have identify the longitudinal trend of the number of papers, journals, organizations and key words in this field. Moreover, based on the nodes' centrality of co-author network, we have found a core journal (i.e. Korean Journal of Oriental Physiology and Pathology), a hub institution (i.e. Kyunghee university) and two main key words (i.e. anti-oxidation and acupuncture) in the research network. In conclusion, integrating field experts' tacit knowledge in Korean medicine studies with the results of the explicit social network analysis on the research trend, we put forward further policy implications with regard to R&D strategies in this field.

• Journal of KIISE
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• v.44 no.3
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• pp.261-274
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• 2017

Variability management is the foremost criterion that defines the extent to which complexities can be handled in a system. Predominantly, the requirements' engineering (RE) study overlooks, or speculates a consistent behavior of, the environment in which a system functions. In real-time systems it is vital to observe and adjust to an intrinsically changing context. Therefore, in this work we identify the requirements of the system in various contexts by recommending a framework using i* goal model, problem frames, use case maps and live sequence charts. The framework is illustrated using a case study of the smart grid RTP system. In the case study, elaboration of scenarios using use case maps and live sequence charts proved beneficial as they assisted in early analysis and validation of contexts. In addition, the elaboration of requirements for obstacle and conflict analysis assists the requirements engineer to increase the robustness of the system. The proposed framework is evaluated theoretically and by empirical study.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.4
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• pp.53-59
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• 2009

The purpose of this research is particle shape evaluation of granular soil and aggregate using Digital Image Process(DIP). DIP is very useful to measure the roughness and particle shape of aggregates. Couple of aggregates, like standard sand, two different crushed stones, and two different marine aggregates, have been employed. Shape factor of two different marine aggregates is ranged 0.35 to 0.54. Crushed stone I is that of 0.74 which is highly flat, but standard sand is elongated shape. Especially, two marine aggregate showed a big difference of width and length which meaned a long shape. There is any significant difference of elongation ratio and flakiness for each aggregate with different measuring system, like direct measurement of vernier calipers and DIP method. Within the limited test results, DIP is one of useful to get the particle shape of aggregate with limitation of measuring errors and to apply the particle distribution curve.

• Journal of Elementary Mathematics Education in Korea
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• v.10 no.2
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• pp.171-194
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• 2006

As it is not long since the gifted education was implemented in elementary school, it is necessary to accumulate the practical studies on the mathematically gifted education. This paper focused on enhancing creativity by providing the various and divergent thinking activities for mathematically gifted students. For this purpose, I prepared two mathematics problems, and , and let the mathematically gifted 5th grade students solve them. After that, I investigated to analyse their reactions in detail and tried to find the methods for assessing their divergent products. Finally, I found that they could pose various and meaningful calculating equations and also identify the various relations between two numbers. I expect that accumulating these kinds of practical studies will contribute to the developments of gifted education, in particular, instructions, assessments, and curriculum developments for the mathematically gifted students in elementary schools.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.2
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• pp.87-92
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• 2010

Large pressure on the growing population has increased rapid change in the LULC (land use/land cover) patterns in the watershed area. Spatial distribution of LULC information and its changes are desirable for any effective planning, managing and monitoring activities. The aim of the study is to produce the 1,50,000 scaled LULC change map for the sub-watershed, Western Moyar, India using the multi-temporal satellite image dataset of IRS LISS III images for the year 1989, 1999, and 2002. About 9 classes are extracted using onscreen visual interpretation techniques for all the three years. The change detection analysis was performed using matrix method for period I (1989-1999) and period II (1999-2002). The study reveals that the changes noticed in period II (1999-2002) is comparatively more than period I (1989-1999), which is dynamic information to protect the sub-watershed area from the deterioration and paves the way to for the sustainable development.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.1
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• pp.42-46
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• 2016

The radioactive iodine ($^{131}I$) presents in the environment through the excrete process of nuclear medicine patients. In the detecting of low level of $^{131}I$ in the public water, the counting uncertainty has an effect on the accuracy and reliability of detecting $^{131}I$ radioactivity concentration. In this study, the contribution of sample amount, radioactivity concentration and counting time to the uncertainty was investigated in the case of public water sample. Sampling points are public water and the effluents of a sewage treatment plant at Sapkyocheon stream, Geumgang river. In each point, 1, 10 and 20 L of liquid samples were collected and prepared by evaporation method. The HPGe (High Purity Germanium) detector was used to detect and analyze emitted gamma-ray from samples. The radioactivity concentration of $^{131}I$ were in the range of 0.03 to 1.8 Bq/L. The comparison of the counting uncertainty of the sample amount, 1 L sample is unable to verify the existence of the $^{131}I$ under 0.5 Bq/L radioactivity concentration. Considering the short half-life of $^{131}I$ (8.03 days), a method for measuring 1 L sample was used. However comparing the detecting and preparing time of 1, 10 L respectively, detecting 10 L sample would be an appropriate method to distinguish $^{131}I$ concentration in the public water.