• 한국수산과학회지
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• 제26권2호
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• pp.111-119
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• 1993

최근 생산량이 증가 추세에 있으며 특히 우리나라 연간생산량 중 거의 절반이 남해안의 전남해역에서 생산되고 있는 새조개의 맛에 기여하는 맛성분을 구명하기 위하여 여수해역에서 생산되고 있는 새조개를 시료로 하여 생육과 자숙육, 그리고 자숙액즙의 함질소엑스화합물 및 무기성분 등을 분석, 비교하였다. 유리 아미노산의 조성은 생육과 자숙육에는 glutamic acid, aspartic acid, glycine, arginine, lysine, leucine 및 alanine의 함량이 가장 많았다. 자숙액즙에는 glutamic acid의 용출율이 가장 높아 $21.7\%$였고 cystine의 용출율이 가장 낮아 $7.3\%$였다. 생육, 자숙육, 자숙액즙 모두 유리아미노산 조성중 필수아미노산은 lysine, arginine 및 leucine의 함유율이 높았고 methionine, histidine 및 tryptophane의 함유율이 낮았다 그리고 비필수 아미노산 중에는 glutamic acid, aspartic acid, glycine 및 alanine의 함유율이 높았고 proline, tyrosine, serine 및 cysteine의 함유율이 낮았다. 핵산관련물질은 생육과 자숙육 모두 ATP, ADP, AMP, inosine 및 hypoxanthine을 함유하고 있었다. 그리고 자숙액즙에는 미량의 ATP, ADP, AMP 및 hypoxanthine이 검출되었다. 핵산관련 물질은 자숙함에 따라 ATP는 약간 감소하였으나 ADP, AMP, inosine 및 hypoxanthine은 약간 증가하였다. TMA 함량은 생육에 $0.3mg\%$이었던 것이 자숙시 $0.8mg\%$로서 다소 증가하였고 TMAO 함량은 생육에 $5.2mg\%$이었던 것이 자숙시 $4.5mg\%$로 다소 감소하였다. betaine함량은 매우 적었다. 무기성분중 P의 함량이 가장 많았고 다음이 K, Ca 및 Zn의 순이었고 Fe, Zn의 함량은 다른 패류에 비해 높았다. 자숙액즙에는 P, Zn 및 Na의 용출율이 높아 각각 $15\%,\;12.2\%$ 및 $11.9\%$였다.

• 한국수산과학회지
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• 제17권5호
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• pp.368-372
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• 1984

보다 간편하게 핵산관련물질을 정량할 목적으로 고속액체크로마토그래피를 이용한 정량법을 검토하였으며, 이 방법을 이용하여 시판 수산건제품의 ATP분해생성물의 함량을 정량하였다. 장치는 HPLC/ALC-244(Waters Associates Inc.)를, 칼럼은 ${\mu}-Bondapak\;C_{18}$ stainless steel column($3.9mm\;i.d.{\times}30.0cm$)을, 검출기는 UV검출기(254nm)를, integrator는 Yanagimoto system-1000을 각각 사용하였으며, 이동상(移動相)으로서는 $1\%$ triethylamine${\codt}$phosphoric acid 혼액 (pH 6.5, $1\%$ triethylamine 용액을 phosphoric acid로써 pH조절)을 사용하고 2ml/min로 용리시키는 것이 가장 분리능이 좋았다. 용리되는 순서는 hypoxanthine, IMP, inosine, AMP, ADP 및 ATP의 순이었으며, retention time 20분이내에 분석이 완료되었다. 그러나 5'-IMP와 5'-GMP는 분리되지 않았다. 자건품(煮乾品)은 이노신산(IMP) 함량이 전반적으로 많았는데, 그 중에서도 벤뎅이가 가장 함량이 많았다. 소건품(素乾品)에는 inosine, hypoxanthine 함량이 많았으며, 조미건제품(調味乾製品)도 소건품과 비슷한 양상이었으나 IMP 함량이 비교적 높은 것은 조미할 때 핵산계조미료가 첨가되었기 때문이라고 볼 수 있었다.

• 한국식품과학회지
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• 제6권3호
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• pp.177-184
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• 1974

살아 있는 말쥐치 Navodon modestus와 까치복 Fugu xanthopterum을 구입(購入)하여 건조중(乾燥中)의 핵산관련물질(核酸關聯物質)의 변화(變化)를 실험(實驗)하였다. 말쥐치는 생시료중(生試料中)에 건량기준(乾量基準)으로 ADP가 22.9 ${\mu}mole/g$로서 가장 많았고, 다음 IMP가 21.1 ${\mu}mole/g,$ ATP는 4.9 ${\mu}mole/g$ hypoxanthine은 3.2 ${\mu}mole/g$ 그리고 AMP와 inosine은 각각(各各) 0.9 ${\mu}mole/g$였다. 까치복 생시료중(生試料中)에도 말쥐치와 마찬가지로 ADP가 건량기준(乾量基準) 25.6 ${\mu}mole/g$로서 가장 많았고, ATP는 2.4 ${\mu}mole/g,$ IMP는 1.6 ${\mu}mole/g$의 순(順)이었으며 AMP, inosine 및 hypoxanthine의 함량(含量)은 아주 적었다. 말쥐치는 열풍건조중(熱風乾燥中) ADP가 급격히 감소(減少)되어 1.4 ${\mu}mole/g$이 되는 반면(反面) inosine이 21.6 ${\mu}mole/g$로 급격히 증가(增加)하였다. IMP는 건조중(乾燥中) 양적(量的)인 큰 변화(變化)가 없었으며, ATP는 건조중(乾燥中) 거의 소실(消失)되었고, AMP는 양(量)은 적지만 2배(倍)로 증가(增加)하였으며, hypoxanthine은 거의 변화(變化)가 없었다. 까치복 역시(亦是) 천일건조중(天日乾燥中) 생시료(生試料) 때 가장 많았던 ADP가 급격히 감소(減少)되어 3.0 ${\mu}mole/g$였고, 반면(反面)에 inosine이 급격히 증가(增加)되어 21.0 ${\mu}mole/g$였다. IMP는 1.6 ${\mu}mole/g$에서 5.0 ${\mu}mole/g,$ AMP는 0.3 ${\mu}mole/g$에서 1.4 ${\mu}mole/g,$ hypoxanthine은 0.4 ${\mu}mole/g$에서 4.9 ${\mu}mole/g$로 각각(各各) 증가(增加)하였고, ATP는 2.4 ${\mu}mole/g$에서 1.4 ${\mu}mole/g$로 감소(減少)하였다. 말쥐치 및 까치복은 inosine축적형(蓄積型)이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제1권4호
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• pp.219-222
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• 1988

Degradation of deoxyribonucleic acid(DNA) and ribonucleic acid(RNA) by cell-free extract of mixed rumen protozoa of buffalo rumen was investigated. DNA was observed to be degraded rapidly during an initial incubation period of 2 hr with simultaneous appearance of degradation products. RNA on the other hand recorded a rapid degradation during an initial incubation period of 1 hr. RNA degradation products appeared upto an incubation period of 2 hr. DNA was observed to degrade into oligo- and mononucleotides. pyrimidine nucleosides, purine nucleoside adenosine and bases xanthine, hypoxanthine and thymine. Degradation products of RNA comprised of pyrimidine nucleosides, purine nucleoside, adenosine and bases xanthine, hypoxanthine and uracil besides oligo- and mononucleotides.

• 대한한의학회지
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• 제21권2호
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• pp.79-86
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• 2000

Objectives and Methods : In order to elucidate toxic mechanism of myocardial damage and protective effect of myrrha water extract against cytotoxic effect of xanthine oxidase/hypoxanthine(XO/HX), cardioprotective effect of myrrha water extract was examined by MTT assay, LDH (Lactate Dehydrogenase) activity and heart beating rate after cultured myocardial cells derived from neonatal mouse were treated with various concentration of XO/HX, a free radical. Results : XO/HX induced a decrease of cell viability, an increase in the amount of LDH, and a decrease of heart beating rate on cultured myocardial cells in a dose-dependent manner. In cardioprotective effect of myrrha water extract, it showed a decrease in the amount of LDH and an increase of heart beating rate on cultured myocardial cells damaged by XO/HX. Conclusions : From the above results, it is suggested that XO/HX showed toxic effect in cultured myocardial cells derived from neonatal mouse and that myrrha water extract is very effective in the prevention of XO/HX-induced cardiotoxicity.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2003년도 제45차 추계학술대회
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• pp.131-132
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• 2003

• 동의생리병리학회지
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• 제16권2호
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• pp.353-358
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• 2002

To test the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity of xanthine oxidase/hypoxanthine (XO/HX) was examined using MTT, TBARS, and beating rate assay in the presence of water extract of Sujeom-san(SJS) or single consituents of its prescription. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present paper, XO/HX resulted in a decrease in viability and beating rate and increases in lipid peroxidation in cultured myocardial cells. In the effect of SJS water extract, it showed effects from the cardiocytotoxicity induced by XO/HX treatment such as increases in beating rate and decreases in lipid peroxidation. In the effect of Rhizoma Corydalis (RC), Faeces Trogopterori (FT), Fructus Amomi Tsaoko (FAT) and Myrrha on the cardiocytotoxicity, they were significantly effective in blocking the XO/HX-induced cardiocytotoxicity by increase of beating rate in FAT and FT group as well as decrease of lipid peroxidation in FT and RC group. These results show that oxygen free radical elicits toxic effects in cultured myocardial cells derived from neonatal rat, and suggest that water extract of Sujeomsan, Rhizoma Corydalis, Faeces Trogopterori, Fructus Amomi Tsaoko or Myrrha is very effective in the prevention of xanthine oxidase/hypoxanthine- induced cardiotoxicity.

• 동의생리병리학회지
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• 제16권4호
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• pp.720-723
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• 2002

To clarify the vasculotoxicity of reactive oxygen intermediate(ROI) in cultured vascular endotherial cells(VEC), of mouse, cytototoxicity was measured by MTS assay after VEC was incubated to 10～80mU/ml xanthine oxidase(XO) and hypoxanthine(HX) for 2 hours. and also, the protective effect of Radix Polygoni Multiflori(RPM) was determined by MTT assay in these cultrures. Cell viability was positively decreased dose-, and time-dependently, after the treatment with 40mU/ml XO/0.1 mM HX to cultured VEC for 2 hours. In the vasculoprotective effect of RPM on the toxicity induced by XO/HX, RPM prevented the XO/HX-induced cytotoxicity in these cultures. From above the results, it suggests that XO/HX is toxic in cultured VEC and herb extract, RPM has protective effect against the vasculotoxicity induced by XO/HX.

• 한국가축번식학회지
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• 제17권2호
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• pp.75-83
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• 1993

Purine has been identified in the preparation of follicular fluid and shown an activity in maintaining oocyte meiotic arrest. Therefore this study was performed to examine the inhibitory effect of purine on germinal vesicle break down(GVBD) in the presence and absence of human fetal cord serum(HFCS) or human mature follicular fluid(HMFF), as a protein source, in vitro culture. Immature oocytes(GV stage) were collected from ovaries of 21∼28 days old ICR mice by puncturing the antral follicles with a fine needle, at 48 hrs after PMSG injection. Some of the oocytes were denuded by drawing the cumulus-enclosed(complex) oocytes in and out of a pasteur pipet. Complex oocytes and denuded oocytes were cultured 3 hrs. in T6 media containing 0.75mM adenosine or/and 4mM hypoxanthine, with HFCS or HMFF. Their GVBD rates were observed at every 1 hr. during the culture time. Both adenosine and hypoxanthine have shown a time-dependent inhibitory effect on GVBD in complex and denuded oocytes and the inhibitory effect was maximized in culture medium containing hypoxanthine and adenosine. HFCS and HMFF increased the GVBD rates in the presence of the purines, thus HFCS and HMFF may contain a factor that could reverse the inhibitory effect of purines. Also complex oocytes were more sensitive to not only the inhibitory effect of purines but the promoting action of HMFF on GVBD than denuded oocytes. Therefore it was reconfirmed that granulosa cells play an important part in meiotic arrest and resumption.

• 한국수산과학회지
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• 제8권3호
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• pp.171-176
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• 1975

개불, Urechis unicinctus, 건조중의 핵산관계물질의 변화를 음이온교환칼럼크로마토그래피로 실험하여 다음과 같은 결과를 얻었다. 살아있는 개불에는 AMP가 건물량기준으로 $6.8\mu\;mole/g$ 로서 가장 함량이 높았고, IMP는 존재하지 않았다. 건조중 ATP, ADP, AMP는 감소하고 inosine과 hypoxanthine은 증가하였으나, AMP이외의 다른 pattern은 함량이 적고 개불에서와 마찬가지로 건조한 개불에 있어서도 AMP가 건물량기준으로 $5\mu\;mole/g$로서 함량이 가장 많았다.