• 한국식품영양과학회지
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• 제32권8호
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• pp.1364-1369
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• 2003

해조 올리고당 함유 음료, 해조 음료 소재 및 해조 추출물 제품을 식이성 고지혈증을 유발시킨 실험동물에 처리하여 체중 증가, 혈중 지질성분 및 간 지질성분의 변화에 미치는 영향을 시험하였다. 식이성 고지혈증을 유도한 흰쥐에 해조 음료 및 해조 추출물 제품을 7일간 투여한 결과, 현저하게 증가되던 체중이 감소하였으며, 체중 대 간장의 무게비도 식이성 고지혈증 유도군에 비해 감소하였다. 혈청중 중성 지질 및 인지질의 함량은 식이성 고지혈증의 유도로 증가되던 것이 해조 음료 및 해조 추출물 제품의 투여로 감소되었으며, 식이성 고지혈증에 의한 혈청중 total-, LDL-, HDL-cholesterol및 동맥경화지수도 해조올리고당의 투여로서 조절되었다. 고지혈증의 유도로 증가되었던 간장 중의 총지질은 해조 음료 및 다시마 추출 음료의 투여로 현저히 감소되었으며, 지질 성분중에서 cholesterol의 함량은 혈청에서와 마찬가지로 크게 감소하였고, 중성 지질(TG, triglyceride)의 함량은 해조 올리고당 음료 식이로 30%와 다시마 추출 음료 식이로 45% 정도가 감소하는 것을 확인하였다.

• Journal of Nutrition and Health
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• 제45권5호
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• pp.420-428
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• 2012

The purpose of this study is to investigate the effects of eight weeks high fat intake and regular exercise in skeletal muscle and adipose tissue for Endoplasmic Reticulum (ER) stress in rats. This experiment involved 32 subjects (sprague-dawley rats) divided into four groups as follows: chow group (Chow, n = 8), chow and exercise group (Chow + EX, n = 8), high fat diet-induced hyperlipidemia group (HF, n = 8), and HF and exercise group (HF + EX, n = 8). As a result, there were significant decrease in body weight and abdominal fat, and blood lipid level was significantly improved by exercise for eight weeks (p < .05). There were variables changed about the skeletal muscle and ER stress in GRP78, XBP-1, ATF4, CHOP and JNK mRNA. There increased in mRNA factor by exercise, especially GRP78, and ATF4 mRNA were significantly increased in exercise (p < .05). However, there were increased in adipose tissue by exercise and there were significantly decreased in mRNA factor by high fat diet (p < .05). Consequently, this study suggests that the consistent exercise was more improved of obesity factor, such as dyslipidemia, hyperlipidemia, hyperglycemia, as well as body weight or abdominal fat. The response of ER stress in adipose tissue and skeletal muscle were more sensitive in exercise than high fat diet feed.

• 한국산학기술학회논문지
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• 제12권11호
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• pp.4940-4950
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• 2011

고지혈증 치료제인 cis-(3R,5R)-atorvastatin Ca (1)은 4개의 입체이성질체가 있으며, 각각의 이성질체들을 선택적으로 제조한다는 것은 매우 어려운 일이다. 본 연구에서는 입체이성질체 중의 하나인 trans-(3R,5S)-atorvastatin Ca (7)을 초산에서 3,5-diketo atorvastatin ester (3)를 $Me_4NHB(OAc)_3$을 사용하여 환원시켜 cis-(3R,5R) (4)과 trans-(3R,5S)-atorvastatin ester (5)를 각각 1.5%와 98.5%의 비율로 입체선택적으로 제조할 수 있었다. 또한, 고지혈증 치료제인 cis-(3R,5R)-atorvastatin Ca (1)과 그의 입체이성질체인 trans-(3R,5S)-atorvastatin Ca (7)을 쥐에서 고지방식이에 의해 유발된 고지혈증의 치료효과에 대하여 알아보았고, 이러한 연구의 수행을 위해 2010년 1월에 실험을 실시하였다. 그 결과, 화합물 1과 7의 total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-c), low-density lipoprotein-cholesterol (LDL-c)과 triglyceride (TG)는 각각 $93.0{\pm}0.5$, $43.5{\pm}0.8$, $40.4{\pm}1.4$, $45.6{\pm}0.9\;mg/d{\ell}$와 $110.0{\pm}0.7$, $33.3{\pm}0.6$, $65.8{\pm}1.9$, $54.8{\pm}1.2\;mg/d{\ell}$를 atherogenic index (AI)와 cardiac risk factor (CRF)는 $1.14{\pm}0.05$ $2.14{\pm}0.05$와 $2.31{\pm}0.06$, $3.31{\pm}0.06$을 나타냈으며 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT)는 $51.9{\pm}4.6$, $16.0{\pm}2.1\;IU/{\ell}$와 $75.8{\pm}4.4$, $35.1{\pm}9.7\;IU/{\ell}$로 두 화합물 모두 고지혈증에 대한 치료효과를 나타내었으나, 화합물 1이 우수한 치료효과가 있는 반면 화합물 7은 치료효과가 낮은 것을 확인하였다. 이러한 결과들로 입체선택적 이성질체의 합성에 대한 새로운 방법을 제시하고, 향후 시판되고 있는 의약품들의 입체이성질체에 대한 임상적 활용이 가능할 것으로 사료된다.

• 대한예방한의학회지
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• 제9권1호
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• pp.105-118
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• 2005

Objectives : The purpose of this study is to investigate the effects of Polygonum multiflorum THUNB(何首烏) on decresing concentration of serum lipid which are triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol. Thiobarbituric Acid Reactive(TBARS) in high cholesterol diet induced hyperlipidemia rats. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), high fat diet administered group(AIN-76 high fat diet #100496 used beef tallow for 4 weeks, Control Group) and Polygonum multiflorum THUNB(何首烏). administered group(AIN-76 high fat diet #100496 used beef tallow and Polygonum multiflorum THUNB(何首烏) extracts 85.0mg/200g 1time/day for 4 weeks, HSO Group). Rats were sacrificed and concentration of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, TBARS were measured in rat's serum lipid. Results : There was no significant difference in the level of weigh between Control and HSO Group.(by Duncan test) Concentration of Total lipid has no significant difference in each group. (F=0.702, p=0.511, ANOVA test) Concentration of triglyceride has no significant difference in each group. (F=0.727, p=0.500, ANOVA test) Concentration of LDL-cholesterol was significantly different in each group.(F=.9.894. p=0.002, ANOVA test) It was significantly increased in the Control group compared with the Normal group and it significantly decreased in the HSO group compared with the Control group. Concentration of HDL-cholesterol has no significant difference in each group. (F=1.079, p=0.365, ANOVA test) TBARS values(Thiobarbituric Acid Reactive Substances) were significantly different in each group.(F=15.580, p=0.0001, ANOVA test) It was significantly increased in the Control group compared with the Normal group and significantly decreased in HSO group compared with the Control group. Conclusions : According to the above results, it is considered that Polygonum multiflorum THUNB(何首烏) is effective in decreasing concentration of serum lipid in high cholesterol diet induced hyperlipidemia rats.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.240-244
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• 2006

To develop a health-aid preparation of Chinese yam (Dioscorea batatas Decne), lactic acid fermentation was attempted using a mixed starter comprising of Lactobacillus acidophilus, Streptococcus thermophilus, and Bifidobacterium bifidus. The anaerobic fermentation of a 5% Chinese yam flour suspension gave a uniform suspension of pH 4.35, containing $7.76{\times}10^6 CFU/mL$ lactic acid bacteria (LAB), and which was found to be acceptable to the panel from a sensory assessment. During the administration of the lactic acid fermented (LAF) Chinese yam to Sprague Dawley rats for 6 weeks, a smaller body weight gain, but greater excretion of feces were observed, implying the creation of a healthy gastrointestine on the administration of LAF Chinese yam, which was also confirmed by the gastrointestinal motility of the feed in rats fed on LAF Chinese yam. The constipation induced by loperamide was further suppressed in a rat group fed on a LAF Chinese yam diet, which was qualified from healthy gastrointestinal flora established by LAB. A serochemical analysis revealed a slight improvement in the blood glucose, neutral lipid and total cholesterol concentrations on administration of LAF Chinese yam, suggesting LAF Chinese yam could be served as a healthy-aid preparation, even for hyperglycemia or hyperlipidemia patients.

• 대한한방내과학회지
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• 제33권3호
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• pp.306-316
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• 2012

Objectives : Mori Folium is used for hyperlipidemia or diabetes. The study was designed to test the anti-obesity effect of Mori Folium on body weight, hepatic fat accumulation and serum lipid level. Methods : The extract from Mori Folium was made by the pharmacy department of Kyung-hee oriental medical hospital. Normal diet, high-fat diet, 30% reduced diet and Mori Folium groups were set. The normal group was administered normal rat food, but the other three groups were administered high fat food. We measured body weight once a week. After 3 weeks experiment, hepatic lipid accumulations were measured. Also we compared serum lipid levels among the 4 groups after 3 weeks. Results : Mori Folium had no effect on body weight, but the 30% reduced diet had an inhibitory effect on body weight gain. Mori Folium had more inhibitory effect on hepatic fat accumulation than a 30% reduced diet. Mori Folium also has more inhibitory effect on serum triglyceride than 30% reduced diet. Conclusions : Mori Folium has anti-obesity effect on hepatic lipid accumulaion and serum triglyceride level in high fat diet induced rats.

• 한국약용작물학회지
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• 제24권5호
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• pp.370-374
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• 2016

Background: Many menopausal women suffer from health problems including metabolic diseases such as dyslipidemia and osteoporosis. Thus they need natural products and functional foods particularly highly nutritional food products, that can help alleviate these diseases. This study was carried out to determine the effect of Drynariae Rhizoma water extract on the lipid and bone metabolism of ovariectomized Sprague-Dawley rats. Methods and Results: The animals were randomly divided into six dietary groups comprising SHAM-operated rats, OVX rats (normal diet), and OVX-DR rats (Drynariae Rhizoma extract). After 8 weeks, plasma, liver, and fat samples were collected to analyze the lipid metabolism, plasma Ca, alkaline phosphatase (ALP), osteocalcin and C-terminal telopeptide (CTx) concentrations, which are biochemical makers of bone metabolism. The left femurs of rats were also collected for histological analyses. OVX counteracted menopause induced body weight gain, as well as increases in triglycerides, total cholesterol, and free fatty acids. The Drynariae Rhizoma group showed low levels of triglycerides, high HDL-cholesterol, and decreased lipogenesis based on activity of the lipid-regulating enzymes (fatty acid synthase and malic enzyme). Decreased serum levels of ALP and osteocalcin were observed in Drynariae Rhizoma group. Conclusions: The results of this study show that Drynariae Rhizoma extract may effectively regulate hyperlipidemia and improve bone density.

• 한국식품영양과학회지
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• 제36권5호
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• pp.548-553
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• 2007

저자들은 일반 흰쥐를 1.5% cholesterol과 0.25% cholic acid, 우지 25% 첨가한 식이로 고지혈증을 유발시킨 후 KH-305를 8주간 경구투여하여 혈중 cholesterol과 HDL-C, LDL-C 등을 측정, 지질대사에 미치는 영향을 알아보았다. 또한, 최대해면체 내압과 내피 관련 NOS 발현정도를 측정하여 음경발기 촉진 및 지속에 미치는 영향을 알아보았다. 그 결과 KH-305를 투여한 모든 그룹에서 고지방식이 그룹보다 총 cholesterol 수치와 LDL-C를 낮추었으며 HDL-C의 수치를 높였다. 최대해면체 내압에서는 KH-305 투여 그룹이 고지방식이 그룹보다 최대해면체 내압에 이르는 시간이 짧았고 최대해면체 내압의 수치가 높았으며 이 중 300mg/kg일 경우 가장 효과가 좋았다. 또한, KH-305 모든 투여 그룹은 고지방식이 그룹과 비교해 볼 때 모든 그룹에서 eNOS와 nNOS의 발현이 뚜렷이 증가되었다. 따라서, 혈중 cholesterol을 낮추면서 NOS의 발현을 증가, 최대해면체 내압이 큰 KH-305는 고지혈증으로 유발된 발기부전에 효능이 있는 약물이 될 수 있으리라 사료된다.

• 생명과학회지
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• 제19권8호
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• pp.1119-1124
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• 2009

포화 지방산이 인슐린 저항성 및 지방간등의 다양한 질병의 발병에 관련된다고 보고되고 있다. 그러나 간세포에서 포화지방산이 세포 사멸에 대한 효과 및 이와 관련된 신호전달계에 대해서는 거의 알려져 있지 않고 있다. 본 연구에서는 대표적인 포화지방산인 palmitic acid 처리 시 랫트 간세포 사멸에 미치는 효과와 이들이 mitogen activated protein kinases (MAPKs)와 관련되는 지를 알아보았다. 본 실험에서 palmitic acid 처리 시 간세포 성장은 억제 되었고 lactate dehydrogenase (LDH) 활성은 증가하였다. 이러한 작용은 JNK 및 p38 MAPK 억제제에 의해서 선택적으로 차단되었다. 아울러 palmitic acid에 의한 Bcl-2 발현 억제, Bax 발현 증가 작용, GSH 함량 감소작용 및 산화성 스트레스 증가작용 역시 JNK 및 p38 MAPK 억제제에 의해서 선택적으로 차단되었다. 실제로 palmitic acid 처리시 JNK 및 p38 MAPK 활성은 증가하였다. 결론적으로 palmitic acid는 간세포에서 JNK 및 p38 MAPK 활성을 경유하여 산화성 스트레스 증가를 통하여 간세포 사멸을 유도하는 것으로 나타났다.

• 대한의생명과학회지
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• 제14권4호
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• pp.211-218
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• 2008

The purpose of this study was to investigate the effects of dietary Chungkookjang (Korean fermented soybean) powder on blood glucose level, lipid profiles, antioxidant enzymes activities and histological changes in kidney of streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats of three groups including nondiabetic group fed normal diet (NC), diabetic group fed normal diet (DC) and diabetic group fed Chungkookjang diet (DCH; 100 g/kg diet) were reared for 8 weeks. The serum glucose, triglycelide and total lipid levels in the DCH group were significantly lower (P<0.05) than the DC group. The renal xanthine oxidase, catalase and glutathione S-transferase activities in the DC group were significantly higher than the NC group. The xanthine oxidase, superoxide dismutase and glutathione S-transferase activities in the DCH group were significantly lower than the DC group (P<0.05). Tubular epithelial change, such as Armanni-Ebstein cells, was significantly reduced in the DCH group compared to the DC group. In conclusion, these results indicated that Chungkookjang supplement seems to be beneficial to correct the hyperglycemia and hyperlipidemia as well as to protect kidney against diabetic changes.