Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Drynariae Rhizoma Hot Water Extract on Metabolic Improvement in the Ovariectomized Rat Model of Menopause

골쇄보 열수추출물의 급여가 갱년기 유도 흰쥐의 대사개선 효능에 미치는 영향

  • Kang, Mi Young (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Chung, Soo Im (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Lee, Sang Chul (School of Applied Biosciences, Kyungpook National University)
  • Received : 2016.06.28
  • Accepted : 2016.10.10
  • Published : 2016.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Many menopausal women suffer from health problems including metabolic diseases such as dyslipidemia and osteoporosis. Thus they need natural products and functional foods particularly highly nutritional food products, that can help alleviate these diseases. This study was carried out to determine the effect of Drynariae Rhizoma water extract on the lipid and bone metabolism of ovariectomized Sprague-Dawley rats. Methods and Results: The animals were randomly divided into six dietary groups comprising SHAM-operated rats, OVX rats (normal diet), and OVX-DR rats (Drynariae Rhizoma extract). After 8 weeks, plasma, liver, and fat samples were collected to analyze the lipid metabolism, plasma Ca, alkaline phosphatase (ALP), osteocalcin and C-terminal telopeptide (CTx) concentrations, which are biochemical makers of bone metabolism. The left femurs of rats were also collected for histological analyses. OVX counteracted menopause induced body weight gain, as well as increases in triglycerides, total cholesterol, and free fatty acids. The Drynariae Rhizoma group showed low levels of triglycerides, high HDL-cholesterol, and decreased lipogenesis based on activity of the lipid-regulating enzymes (fatty acid synthase and malic enzyme). Decreased serum levels of ALP and osteocalcin were observed in Drynariae Rhizoma group. Conclusions: The results of this study show that Drynariae Rhizoma extract may effectively regulate hyperlipidemia and improve bone density.

Keywords

References

  1. Ahn DK. (2003). Illustrated book of Korean medicinal herbs. Kyohaksa. Seoul, Korea. p.954.
  2. Bieber LL, Abraham T and Helmrath T. (1972). A rapid spectrophotometric assay for carnitine palmitoyltransferase. Analytical Biochemistry. 50:509-518. https://doi.org/10.1016/0003-2697(72)90061-9
  3. Bijlani RL, Vempati RP, Yadav RK, Ray RB, Gupta V, Sharma R, Mehta N and Mahapatra SC. (2005). A brief but comprehensive lifestyle education program based on yoga reduces risk factors for cardiovascular disease and diabetes mellitus. The Journal of Alternative and Complementary Medicine. 11:267-274. https://doi.org/10.1089/acm.2005.11.267
  4. Bone HG, Bolognese MA, Yuen CK, Kendler DL, Wang H, Liu Y and San Martin J. (2008). Effects of denosumab on bone mineral density and bone turnover in postmenopausal women. The Journal of Clinical Endocrinology and Metabolism. 93:2149-2157. https://doi.org/10.1210/jc.2007-2814
  5. Choi JH, Kim YJ and Rhee SJ. (2001). Effects of green tea catechin on bone disorder in long-term cadmium treated rats. Journal of the Korean Society of Food Science and Nutrition. 30:1253-1259.
  6. Gibson DM and Hubbard DD. (1960). Incorporation of malonyl CoA into fatty acids by liver in starvation and alloxan-diabetes. Biochemical and Biophysical Research Communications. 3:531-535. https://doi.org/10.1016/0006-291X(60)90169-8
  7. Graffagnino CL, Falko JM, Londe ML, Schaumburg J, Hyek MF, Shaffer LET, Snow R and Caulin-Glaser T. (2006). Effect of a community-based weight management program on weight loss and cardiovascular disease risk factors. Obesity. 14:280-288. https://doi.org/10.1038/oby.2006.36
  8. Hong SN and Jeong JC. (2000). Effects of complex extracts having Drynariae Rhizoma on suppression of collagenolysis and bone resorption in mouse calvarial osteoblasts. Dongguk Journal of The Institute of Oriental Medicine. 9:179-191.
  9. Hulcher FH and Oleson WH. (1973). Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. Journal of Lipid Research. 14:625-631.
  10. Jeong JC, Kang SK, Youn CH, Jeong CW, Kim HM, Lee YC, Chang YC and Kim CH. (2003). Inhibition of Drynariae Rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts. International Immunopharmacology. 3:1685-1697. https://doi.org/10.1016/j.intimp.2003.08.003
  11. Jeong JC, Lee BT, Yoon CH, Kim HM and Kim CH. (2005). Effects of Drynariae Rhizoma on the proliferation of human bone cells and the immunomodulatory activity. Phamacological Research. 51:125-136.
  12. Kang MY, Lee SH, Lee SW, Cha SW, Song JL and Lee SC. (2015). Effect of Achyranthis Radix and Drynariae Rhizoma extracts on antioxidant activity and antioxidant enzymes. Korean Journal of Plant Resources. 28:600-607. https://doi.org/10.7732/kjpr.2015.28.5.600
  13. Kim KJ and Jeong JC. (2001). Effects of Rhizoma Davalliae on bone metabolism in ovariectomized rats. Korean Journal of Oriental Internal Medicine. 22:175-181.
  14. Mastorakos G, Valsamakis G, Paltoglou G and Creatsas G. (2010). Management of obesity in menopause: Diet, exercise, pharmacotherapy and bariatric surgery. Maturitas. 65:219-224. https://doi.org/10.1016/j.maturitas.2009.12.003
  15. Ochoa S. (1955). Methods in enzymology: Malic dehydrogenase from pig heart. In Colowick SP and Kaplan NO(ed.). Academic Press. New York, NY, USA. p.735-739.
  16. Oh CS and Lee H. (2008). A Study on the effect of herbalacupuncture with Drynariae Rhizoma infusion solution at Umgok(KI10) on osteoporotic rats induced by ovariectomy. The Journal of Korean Acupuncture and Moxibustion Society. 25:71-86.
  17. Peled E, Davis M, Axelman E, Norman D and Nadir Y. (2013). Heparanase role in the treatment of avascular necrosis of femur head. Thrombosis Research. 131:94-98. https://doi.org/10.1016/j.thromres.2012.09.018
  18. Picard F, Deshaies Y, Lalonde J, Samson P, Labrie C, Belanger A, Labrie F and Richard D. (2000). Effects of the estrogen antagonist EM-652.HCl on energy balance and lipid metabolism in ovariectomized rats. International Journal of Obesity. 24:830-840. https://doi.org/10.1038/sj.ijo.0801240
  19. Rivero TD and Bethea JR. (2016). The effects of spinal cord injury on bone loss and dysregulation of the calcium/parathyroid hormone loop in mice. Osteoporosis and Sarcopenia. 2:164-169. https://doi.org/10.1016/j.afos.2016.06.003
  20. Sun JS, Lin CY, Dong GC, Sheu SY, Lin FH, Chen LT and Wang YJ. (2002). The effect of Gu-Sui-Bu(Drynaria fortunei J. Sm) on bone cell activities. Biomaterials. 23:3377-3385. https://doi.org/10.1016/S0142-9612(02)00038-8
  21. Xu MS, Shen YG, Wu HL, Shu HG, Wang WJ and Shang Guan XC. (2004). The hypoglycemic effects of Cyclocarya paliurus(Batal.) iljinsk water extracts on diabetes mice. Acta Nutrimenta Sinica. 26:230-231.