• Proceedings of the Korea Contents Association Conference
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• 2004.11a
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• pp.427-433
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• 2004

Most information providers are offering integrated retrieval service based on the bibliographic metadata and schema corresponding to each type of document which are developed in a distributed and independent way. However, it is difficult to maintain the relational consistency of those single heterogeneous databases even though they obey the metadata standard like MARC or MODS. It is the main reason that those standards are restricted to present the general property of document regardless of its type and not to applied to define the relationship of document types. Therefore, It is necessary to define a comprehensive meta model to associate the related databases in a systematic way so that the semantically common part of them can be easily shared and reused without any additional effort like conversion or mapping. In this paper, we first outline the document types for designing meta model by the empirical analysis of various data schema of main information providers. We propose then data element definition, metadata model and modularized XML DTD which support the efficient and consistent management of multiple ducument types.

• Proceedings of the Korea Contents Association Conference
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• 2004.11a
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• pp.516-530
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• 2004

With the advent of knowledge-based society where knowledge generates power, the importance of information is growing than ever before. However, it is not easy to find necessary information in time. Collecting, processing and disseminating valuable information is getting more and more important. If those information include scientific and technical issues, it is more difficult to connect the sender and receiver, provider and consumer and also creates various factors and situation. This study aims to provide an application method of IMC(Integrated Marketing Communication) Strategy for efficient information services of science and technology which is one of KISTI's major functions. An analysis was proceeded in 3 categories with provider and receiver, and medium and message. The current problem in system was reviewed through each analysis and a feasible plan for the realization of IMC Strategy in future is proposed.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.669-672
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• 2010

본 연구는 방문건강관리사업 대상자 중 취약한 노인여성과 노인증후군 관련요인에 대한 인과관계 모형을 규명하여 취약한 노인 여성의 보건의료서비스 제공 시 효율적인 방안제시를 위한 기초자료로 활용하고자 시행하였다. 연구대상자는 2008년 7월 1일부터 9월 30일까지의 기간 동안 전국 보건소에 등록된 방문건강관리사업 대상자 중 동의된 65세 이상 노인여성 1,350명을 선정하여 훈련된 조사원이 직접면담을 통해 조사하였다. 수집된 자료 중 불충분한 29명을 제외한 1,321명을 최종 분석대상으로 하였다. 수집된 자료는 SPSS 17.0 프로그램을 이용하여 빈도분석 및 카이제곱 검정, t-검정, ANOVA, 사후검정은 Tukey를 이용하였으며, 유의한 변수들을 선정하여 로지스틱 회귀분석을 이용하여 분석 하였다. 연구결과를 요약하면 다음과 같다. 첫째, 허약과 관련 있는 노인증후군위험요인은 우울, 요실금, 낙상력, 일상적 수행활동(ADL)으로 나타났다(p<0.05). 둘째, 허약에 영향을 미치는 요인으로는 연령이 75세 이상의 고령일수록, 우울과 요실금의 증상이 있을 때, 최근 낙상경험이 있을 때 허약의 증가 위험이 높은 것으로 나타났다(p<0.05). 셋째, 노인증후군의 위험요인 증가에 영향을 미치는 요인으로는 평생 동안 담배를 5갑 이상 피웠을 때, 당뇨병이 있을 때로 나타났다(p<0.05). 이상 결과를 볼 때, 취약 노인 대상으로 노인증후군, 허약의 향상을 위해서는 만성질환관리 프로그램 뿐 만 아니라 체계적인 근력강화 운동 및 정신건강관리 프로그램의 충분한 확대보급이 반드시 필요하다.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.307-312
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• 2009

The study of candidate genes is an important tool to identify genes associated with economic traits. Three genes were selected to study the association between polymorphism and economic traits and breed of pigs. Growth hormone releasing hormone (GHRH) takes part in growth metabolism and is one of the candidate genes known to be highly associated with economic traits in pigs. Heart fatty acid-binding protein (H-FABP) is related to back fat thickness and intramuscular fat (IMF) content, and myogenin (MYOG) is associated with the amount of growth rate and lean yield in pigs. By PCR-RFLP analysis, the association between the genotypes of the three genes and the average daily gain, back fat thickness, feed conversion, body length and meat percent in 352 pigs (112 Duroc pigs, 132 Landrace pigs and 108 Yorkshire pigs) were analyzed. GHRH polymorphisms showed differences depending on breed (p<0.01) and were associated with meat percent. H-FABP polymorphisms also showed significant differences among breeds and sex (p<0.01), and were highly associated with average daily gain, feed conversion and back fat thickness (p<0.01) and even showed an association with meat percent (p<0.05). However, the MYOG gene showed no significant effect in this study. These results reconfirmed that GHRH and H-FABP are potential major genes or markers for economic traits.

• Microbiology and Biotechnology Letters
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• v.25 no.4
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• pp.359-366
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• 1997

The usefulness of host range expanded recombinant viruses for economical viral insecticide and expression vector system has been studied. Host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and Autographa californica NPV (AcNPV), and a host range expanded AcNPV recombinant, Ac-BH, constructed by substitution of the 0.6Kb fragment of the BmNPV helicase gene were compared. The restriction enzyme digestion patterns showed that RecS-B6 and RecB-8 had expanded host ranges by genomic recombination and were more similar to genome of AcNPV than that of BmNPV. SDS-PAGE and PCR analysis showed that the polyhedrin gene of RecS-B6 and RecB-8 was derived from BmNPV genomic DNA. The morphology of polyhedra of recombinant viruses showed a slight difference between the two host cells, Sf and BmN cells, indicating that the morphology of polyhedra was influenced by host cells. The bioassay data for insect larvae showed that Ac-BH, compared to wild type viruses, had superior pathogenicity against Bombyx mori larvae but inferior pathogenicity against Spodoptera exigua larvae. Although the pathogenicity was lower than that of wild type viruses in both larvae, RecS-B6 showed the pathogenicity in both larvae. These results suggested that Ac-BH was a less useful economical insecticide than random genomic recombinant virus RecS-B6.

• Journal of Microbiology and Biotechnology
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• v.19 no.2
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• pp.128-135
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• 2009

Acinetohacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene was cloned and expressed in Escherichia coli BL21(trxB) as an inclusion body, which was subsequently solubilized by urea, and then purified using Ni-affinity chromatography. After being purified, the lipase was refolded by incubation at $4^{\circ}C$ in the presence of a 1:10 molar ratio of lipase:chaperone. The maximal activity of the refolded lipase was observed at a temperature of $35^{\circ}C$ and pH 8.3 when p-NP caprate(C10) was used as a substrate; however, 28% of the activity observed at $35^{\circ}C$ was still remaining at $0^{\circ}C$. The stability of the purified enzyme at low temperatures indicates that it is a cold-adapted enzyme. The refolded lipase was activated by $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$, whereas $Zn^{2+}\;and\;Cu^{2+}$ inhibited it. Additionally, 0.1% Tween 20 increased the lipase activity by 33%, but SDS and Triton X-100 inhibited the lipase activity by 40% and 70%, respectively.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1180-1188
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• 2017

Neuronatin (NNAT) is known to regulate ion channels during brain development and plays a role in maintaining the structure of the nervous system. A previous in silico analysis showed that Nnat was overexpressed in the adipose tissue of an obese rodent model relative to the wild type. Therefore, the aim of the present study was to investigate the function of Nnat in the adipose tissue. Because obesity is known to systemically induce low-grade inflammation, the Nnat expression level was examined in the adipose tissue obtained from C57BL/6 mice administered lipopolysaccharide (LPS). Unexpectedly, the Nnat expression level decreased in the white adipose tissue after LPS administration. To determine the role of NNAT in inflammation, 3T3-L1 cells overexpressing Nnat were treated with LPS. The level of the p65 subunit of nuclear factor-kappa B ($NF-{\kappa}B$) and the activity of $NF-{\kappa}B$ luciferase decreased following LPS treatment. These results indicate that NNAT plays an anti-inflammatory role in the adipose tissue.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.3
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• pp.249-254
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• 2014

The purpose of this study is to characterize the effects of KHG26792 (3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride), a potential skin whitening agent, on melanin synthesis and identify the underlying mechanism of action. Our data showed that KHG26792 significantly reduced melanin synthesis in a dose-dependent manner. Additionally, KHG26792 downregulated microphthalmia-associated transcription factor (MITF) and tyrosinase, the rate-limiting enzyme in melanogenesis, although tyrosinase was not inhibited directly. KHG26792 activated extracellular signal-regulated kinase (ERK), whereas an ERK pathway inhibitor, PD98059, rescued KHG26792-induced hypopigmentation. These results suggest that KHG26792 decreases melanin production via ERK activation. Moreover, the hypopigmentary effects of KHG26792 were confirmed in a pigmented skin equivalent model using Cervi cornus Colla (deer antler glue), in which the color of the pigmented artificial skin became lighter after treatment with KHG26792. In summary, our findings suggest that KHG26792 is a novel skin whitening agent.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.5
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• pp.547-554
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• 2017

Previous studies have demonstrated the role of hydroquinone (HQ), a hydroxylated benzene metabolite, in modulating various immune responses; however, its role in macrophage-mediated inflammatory responses is not fully understood. In this study, the role of HQ in inflammatory responses and the underlying molecular mechanism were explored in macrophages. HQ down-regulated the expression of interferon $(IFN)-{\beta}$ mRNA in LPS-stimulated RAW264.7 cells without any cytotoxicity and suppressed interferon regulatory factor (IRF)-3-mediated luciferase activity induced by TIR-domain-containing adapter-inducing interferon-${\beta}$ (TRIF) and TANK-binding kinase 1 (TBK1). A mechanism study revealed that HQ inhibited IRF-3 phosphorylation induced by lipopolysaccharide (LPS), TRIF, and AKT by suppressing phosphorylation of AKT, an upstream kinase of the IRF-3 signaling pathway. IRF-3 phosphorylation is highly induced by wild-type AKT and poorly induced by an AKT mutant, AKT C310A, which is mutated at an inhibitory target site of HQ. We also showed that HQ inhibited IRF-3 phosphorylation by targeting all three AKT isoforms (AKT1, AKT2, and AKT3) in RAW264.7 cells and suppressed IRF-3-mediated luciferase activities induced by AKT in HEK293 cells. Taken together, these results strongly suggest that HQ inhibits the production of a type I IFN, $IFN-{\beta}$, by targeting AKTs in the IRF-3 signaling pathway during macrophage-mediated inflammation.

• Journal of Advanced Navigation Technology
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• v.15 no.1
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• pp.11-17
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• 2011

In this paper, we consider the frequency sharing feasibility of Fixed Satellite Service (FSS) with Microwave Landing System (MLS) allocated in the C-band. On the basis of characteristics of MLS and FSS suggested by ITU and safety standards of MLS by ICAO, three interference scenarios are built and numerical simulations are carried out for investigating the frequency sharing between the two systems. From the simulation results, we calculate the separation distance between MLS and FSS ground antennas, required for frequency sharing by employing standard protection limits regulating the interference to noise ratio (I/N) of the receiver.