• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1809-1818
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• 2008

Biotechnological treatment of sulfate- and metal-ions-containing acidic wastewaters from mining and metallurgical activities utilizes sulfate-reducing bacteria to produce sulfide that can subsequently precipitate metal ions. Reducing sulfate at a low pH has several advantages above neutrophilic sulfate reduction. This study describes the effect of sulfide removal on the reactor performance and microbial community in a high-rate sulfidogenic gas-lift bioreactor fed with hydrogen at a controlled internal pH of 5. Under sulfide removal conditions, 99% of the sulfate was converted at a hydraulic retention time of 24 h, reaching a volumetric activity as high as 51 mmol sulfate/l/d. Under nonsulfide removal conditions, <25% of the sulfate was converted at a hydraulic retention time of 24 h reaching volumetric activities of <13 mmol sulfate/l/d. The absence of sulfide removal at a hydraulic retention time of 24 h resulted in an average $H_2S$ concentration of 18.2 mM (584 mg S/I). The incomplete sulfate removal was probably due to sulfide inhibition. Molecular phylogenetic analysis identified 11 separate 16S rRNA bands under sulfide stripping conditions, whereas under nonsulfide removal conditions only 4 separate 16S rRNA bands were found. This shows that a less diverse population was found in the presence of a high sulfide concentration.

• Environmental Engineering Research
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• v.21 no.1
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• pp.69-73
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• 2016

Generation of food waste is a serious issue that needs to be addressed worldwide. Developing suitable treatment methods while generating energy (methane) is a common practice for sustainable treatment of waste. In this study, methane generation by food waste was investigated in mesophilic and thermophilic regimes at various hydraulic retention times (HRTs) and organic loading rates (OLR). In temperature regimes, influent concentrations and HRTs ranged from 30 to 110 g COD/L and 18 to 30 days, respectively, which corresponding to an OLR of 1.0 to $6.1kg\;COD/m^3-d$. Better methane production and organic removal was observed under thermophilic conditions because of the enhanced hydrolysis of complex polymers and microbial activity at higher temperature. The peak methane productivities attained in thermophilic and mesophilic regimes were 1.30 and $0.99m^3/m^3-d$, respectively. The maximum methane yields were achieved at 50 g COD/L and HRT of 24 d in both cases, and the values were 264 and $221m^3/ton$ COD, respectively. The results of this study will facilitate the development of sustainable methane production technologies using food waste as a feedstock.

• Journal of Korean Society on Water Environment
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• v.25 no.1
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• pp.120-124
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• 2009

This study was performed to investigate the application of submerged membrane bioreactor (MBR) system for biological nutrient removal of municipal wastewater. MBR bioreactor consists of four reactors such as anaerobic, stabilization, anoxic and submerged membrane aerobic reactors with two internal recycles. The hydraulic retention time (HRT), sludge retention time (SRT) and flux were 6.2 hr, 34.1 days and $19.6L/m^2/hr$ (LMH), respectively. As a result of operation, the removal efficiency of $COD_{Cr}$, SS, TN and TP were 94.3%, 99.9%, 69.4%, and 74.6%, respectively. There was no significant effect of microbial activity after the maintenance cleaning using 200 mg/L of NaOCl. Membrane filtration for the treatment of municipal wastewater was performed for longer than 9 months without chemical recovery cleaning.

• Restorative Dentistry and Endodontics
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• v.45 no.1
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• pp.3.1-3.10
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• 2020

Objectives: This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs) through different dentin thicknesses. Materials and Methods: Two-chamber setups were designed to simulate indirect pulp capping (IPC). Human molars were sectioned to obtain 0.1-, 0.3-, and 0.5-mm-thick dentin discs, which were placed between the 2 chambers to simulate an IPC procedure. Then, MTA and CEM were applied on one side of the discs, while hDPSCs were cultured on the other side. After 2 weeks of incubation, the cells were removed, and cell proliferation, morphology, and attachment to the discs were evaluated under scanning electron microscopy (SEM). Energy-dispersive X-ray (EDXA) spectroscopy was performed for elemental analysis. Alkaline phosphatase (ALP) activity was assessed quantitatively. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: SEM micrographs revealed elongated cells, collagen fibers, and calcified nucleations in all samples. EDXA verified that the calcified nucleations consisted of calcium phosphate. The largest calcifications were seen in the 0.1-mm-thick dentin subgroups. There was no significant difference in ALP activity across the CEM subgroups; however, ALP activity was significantly lower in the 0.1-mm-thick dentin subgroup than in the other MTA subgroups (p < 0.05). Conclusions: The employed capping biomaterials exerted biological activity on hDPSCs, as shown by cell proliferation, morphology, and attachment and calcific precipitations, through 0.1- to 0.5-mm-thick layers of dentin. In IPC, the bioactivity of these endodontic biomaterials is probably beneficial.

• The Journal of Advanced Prosthodontics
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• v.9 no.3
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• pp.217-223
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• 2017

PURPOSE. This study investigated the effects of silver nanoparticle (SN) loading into hydraulic calcium silicate-based Portland cement on its mechanical, antibacterial behavior and biocompatibility as a novel dental bone substitute. MATERIALS AND METHODS. Chemically reduced colloidal SN were combined with Portland cement (PC) by the concentrations of 0 (control), 1.0, 3.0, and 5.0 wt%. The physico-mechanical properties of silver-Portland cement nanocomposites (SPNC) were investigated through X-ray diffraction (XRD), setting time, compressive strength, solubility, and silver ion elution. Antimicrobial properties of SPNC were tested by agar diffusion against Streptococcus mutans and Streptococcus sobrinus. Cytotoxic evaluation for human gingival fibroblast (HGF) was performed by MTS assay. RESULTS. XRD certified that SN was successfully impregnated in PC. SPNC at above 3.0 wt% significantly reduced both initial and final setting times compared to control PC. No statistical differences of the compressive strength values were detected after SN loadings, and solubility rates of SPNC were below 3.0%, which are acceptable by ADA guidelines. Ag ion elutions from SPNC were confirmed with dose-dependence on the concentrations of SN added. SPNC of 5.0 wt% inhibited the growth of Streptococci, whereas no antimicrobial activity was shown in control PC. SPNC revealed no cytotoxic effects to HGF following ISO 10993 (cell viability > 70%). CONCLUSION. Addition of SN promoted the antibacterial activity and favored the bio-mechanical properties of PC; thus, SPNC could be a candidate for the futuristic dental biomaterial. For clinical warrant, further studies including the inhibitory mechanism, in vivo and long-term researches are still required.

• Journal of the Korean Society for Precision Engineering
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• v.14 no.12
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• pp.95-104
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• 1997

We consider controller design problem to enhance shift quality for automatic transmission. A dynamic modeling related to shifting (mainly 2-3 up-shift) is constructed and nonlinear robust controllers are designed to reduce output torque during shifting. Suggesting a new hydraulic circuit enabling the direct clutch drive, the control activity is extended and more implementable than the conventional design. The designed robust controllers overcome the unmodeled dynamics and the uncertainty embending in the system. Moreover, the dynamic effect between the clutch pressure and the PWM valve duty is considered via singular perturbation technique.

• Magazine of the Korean Society of Agricultural Engineers
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• v.35 no.3
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• pp.74-80
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• 1993

Runoff simulation tests to investigate the flow mechanics of nonsuomerged overland flow in a natural grass intervening land system were condueted and a modified kinematic wave overland runoff model developed by Choi et al. (1993) was verified. Nonhomogeneity and heterogeneity of the soil, slope, local topography, infiltration, grass density, and the density and activity of the soil microhes and wild animals were the major factors affecting the flow. Streamlines were disturbed by grass stems and small concentrated flows due to the disturbed streamlines and local topography were observed a lot. Relatively larger concentrated flows were observed where bundles of grass were dominant than where individual grasses were growing. Predicted hydrographs were agreed verv well with measured hydrographs. Since the modified model considers grass density in computing flow depth and hydraulic radius, it can be better than existing kinematic wave model if it were used to route nonpoint source pollutant attenuation processes in many grass intervening land systems.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.11a
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• pp.773-776
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• 2005

MSWI ash is the residue from waste combustion processes at temperature between $850^{\circ}C\;and\;1000^{\circ}C$. And the main components of MSWI ash are $SiO_2,\;CaO\;and\;Al_2O_3$. The aim of this study is to find a way to useful application of MSWI ash(after treatment) as a structural material and to investigates the hydraulic activity, compressive strength development, composition variation of such chemicallyi-activated MSWI ashes concrete. And it was found that early cement hydration, followed by the breakdown and dissolving of the MSWI-ashes, enhanced the formation of calcium silicate hydrates(C-S-H), The XRD and SEM-EDS results indicate that, both the hydration degree and strength development are closely connected with a curing condition and a chemically-activator. Compressive strengths with values in the 40.5MFa were obtained after curing the activated MSWI ashes with NaOH+water glass at $90^{\circ}C$.

• Journal of Energy Engineering
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• v.27 no.1
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• pp.59-64
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• 2018

In this paper, we investigated the alternative sources of limestone. Oyster shell waste originated from aquaculture that causes a major disposal landfill problem in coastal sectors in southeast Korea. Their inadequate disposal causes a significant environmental problems araised. Bio mineralization leads to the formation of oyster shells and consists $CaCO_3$ as a major phase with a small amount of organic matter. It is a good alternative material source instead of natural lime stone. The utilization of oyster shell waste for industrial applications instead of natural limestone is major advantage for conservation of natural limestone. The present work describes the limestone and oyster shells hydraulic activity and chemical composition and characteristics are most similar for utilization of oyster shell waste instead of natural limestone.

• Journal of Korean Society of Water and Wastewater
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• v.30 no.3
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• pp.293-297
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• 2016

This study was performed in order to obtain the effect of the compressive strength of the cured product with manufacturing conditions (amounts of fine aggregate and different types of alkali activator). Material which is the basis of the cured product was used for the blast furnace slag, which has a latent hydraulic activity. Consequently, when using sodium hydroxide as the alkali activator, it is possible to obtain a higher compressive strength than using the calcium hydroxide. And also, it can be added a 10% of fine aggregate with blast furnace slag to improve the compressive strength.