• The Korean Journal of Mycology
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• v.49 no.1
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• pp.119-125
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• 2021

Hydrangea petiolaris, belonging to the family Hydrangeaceae, is a vine plant distributed in Ulleung, Jeju, and other southern islands of Korea. In October 2017, a rust fungus was discovered on H. petiolaris in Jeju Island, Korea. To identify the rust fungus, we performed a morphological examination and molecular phylogenetic analysis of the internal transcribed spacer and 28S large subunit rDNA sequences. As a result, the fungus was identified as Pucciniastrum hydrangeae-petiolaris, consistent with previous reports from Japan and Russia, but showed a significant phylogenetic distance from Pucciniastrum hydrangeae reported on Hydrangeae spp. in North America. This is the first record of P. hydrangeae-petiolaris on H. petiolaris in Korea.

• Korean Journal of Plant Taxonomy
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• v.34 no.1
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• pp.1-7
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• 2004

An unrecorded species in Korean flora, Hydrangea luteovenosa Koidz. (Hydrangeaceae) was collected from parasitic cone, Seongneol-orom region of Mt. Halla in Prov. Jeju-do. This species is readily distinguishable from H serrata (Thunb.) Ser. and H. petiolaris Siebold & Zucc. in Korea by habit, length and width of leaves, length of petiole, morphology of petals and leaf margins, position of ovary, number of stamens and secondary veins.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.2
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• pp.123-134
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• 2022

In this study, the whitening and antioxidant effects of the extracts from Hydrangea petiolaris (H. petiolaris) leaves was confirmed, and the chemical structure was identified by separating the active ingredients. In the whitening tests using α-MSH stimulated B16F10 melanoma cells, the n-hexane (Hex) fraction inhibited the cellular melanogenesis and intracellular tyrosinase activities without causing cell toxicity. In addition, the Hex fraction reduced expression of tyrosinase and TRP-2 protein. Upon the anti-oxidative studies by DPPH and ABTS⁺ radicals, potent radical scavenging activities were observed in the ethyl acetate (EtOAc) fraction. Also, for the cellular protective effects on HaCat keratinocytes damaged by H₂O₂, the EtOAc fraction indicated protective effects against oxidative stress. Eight phytochemicals were isolated from the extract of H. petiolaris leaves; ethyl linoleate (1), ethyl linolenate (2), 1-linoleoyl glycerol (3), 1-linolenoyl glycerol (4), epi-catechin (5), afzelin (6), quercitrin (7), hyperin (8). Among the isolates, the compounds 5 - 8 showed DPPH and ABTS⁺ radical scavenging activities. The contents of quercitirin, a major isolated in this extract, determined by HPLC analysis were confirmed to be about 31.3 mg/g for the 70% ethanol extract and 169.8 mg/g for the EtOAc fraction. Based on these results, it was suggested that the extract from H. petiolaris leaves could be potentially applicable as whitening and anti-oxidative ingredients in cosmetic formulations.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.407-418
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• 2016

In this study, the phylogenetic relationship of Korean Hydrangea was evaluated by using sequenced three chloroplast regions and ITS region, including the 7 taxa. The result of phylogenetic analysis indicated that Korean Hydrangea, 7 taxa formed the monophyletic group. This analysis also revealed that subsect. Macrophyllae of Korea was separated into two groups; H. serrata f. acuminate and H. macrophylla group. The H. serrata f. acuminta group was included with H. serrata f. buergeri and H. serrata f. fertilis. These three species form a monophyletic clade, with no significant differences between their nucleotide sequences. The H. serrata f. acuminta group showed a monophyletic group with H. serrata f. buergeri and H. serrata f. fertilis and there is significant differences between their nucleotide sequences. H. macrophylla group was an independent clade distinguished by H. serrate f. acuminate group. Subsect. Petalanthe, Heteromallae and Calyptranthae form a monophyletic group. H. petiolaris which is located in Subsect. Calyptranthae was separated into two subgroups; First subgroup: Jeju island (except for Mt. Halla) and Second subgroup: Ulleung island and Japan. Additional studies of two subgroups of H. petiolaris should be conducted a geographical study and add more samples.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.3
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• pp.207-218
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• 2020

In this study, the anti-inflammatory and anti-bacterial activities of the extracts from the leaves of the Hydrangea petiolaris were identified, and the chemical structure was identified by separating the active ingredient. As the result of the anti-inflammatory activity experiment using RAW 264.7 cells, it was confirmed that the n-hexane (Hex) and ethyl acetate (EtOAc) fractions inhibited the production of nitric oxide (NO) and the expression of iNOS protein in a concentration-dependent manner without cytotoxicity. In addition, the n-Hex and EtOAc fractions reduced the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6). Upon the anti-bacterial tests using Staphylococcus epidermidis and Cutibacterium acnes, the extract, n-Hex, EtOAc and n-butanol (BuOH) fractions showed potent activities. In order to isolate the active constituents, the n-Hex and EtOAc fractions were further purified to afford four phytochemicals; phytol (1), corosolic acid (2), asiatic acid (3) and 1-O-p-coumaroyl-β-D-glucopyranoside (4). All of the compounds 1 - 4 were isolated for the first time from this plant. In addition, the contents of isolated compounds were determined by HPLC and the quantity of phytol (1) was 27.8 mg/g for the 70% EtOH extract. Based on the above research results, it is believed that it will be possible to develop a natural cosmetic material that has anti-inflammatory and anti-bacterial effects using the extract of H. petiolaris leaves.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.534-539
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• 2014

This study was carried out to establish seed propagation method of Hydrangea petiolaris Siebold & Zucc., one of the specific and protected plant species for the floral region. Seed size ranged $1.36{\times}0.84mm$, weight of thousand seeds was $2.3{\pm}0.02mg$. Observation of seed morphology characters showed wrinkled surface and elliptical shape, indicating amber color and fine seeds. Moisture contents of seeds, increased rapidly by water-soaking treatment, recording maximum moisture contents (31.9%) after 48 hours. Percent germination of seeds was higher under the light than dark condition. Seed germination was the best at $25^{\circ}C$ under the light condition (78.0%) among temperature and light conditions treated. Percent germination, germination energy and $T_{50}$ was greatly improved by soaking in $GA_3$ solutions for 48 hours. Especially, $100{\sim}500mg{\cdot}L^{-1}$ $GA_3$ treatment resulted in the highest germination rate as 90.0% and above.

• Journal of Environmental Science International
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• v.12 no.1
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• pp.15-22
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• 2003

Fifteen major naturally populated habitats around the Mt. Halla, Jeju province for wild Cornus kousa are mostly located on the southeast side of the mountains at 850-1,550m in altitude at sea level and are in half sunney areas. More than 40.0% of the naturally populated area of Cornus kousa are edge of forest areas and 53.3% populate on the steep slope of the mountain in 0-5℃. The direction of the slop for about 40.0% of total populated areas is southeast. In the naturally populated areas, total of 64 taxa dividing into 42 families, 59 genera 56 species and 8 varieties have been shown, mostly Hydrangea petiolaris, Rubus oldhamii, Ilex crenata, Sasa quelpaertensis, Ligustrum obtusifolium. It is suggested that these areas are secondary forest in a typical deciduous forest in Mt. Halla where the second transition had been progressed after forest damage or environmental stress. The index of species diversity of plant groups in these areas is 1.286-4.238 based on the Shannon-Wiener's method.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.491-501
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• 2021

This study was carried out to investigate the occurrence of seedlings of Korean fir (Abies koreana) in Korean fir forest located on the eastern slope of Mt. Halla National Park. Between June and August in 2016, the systematic sampling method was applied to 125 plots of 5 m × 5 m quadrats and field survey was came out to measure the characteristics of sites and understory vegetation. Correlation analysis was applied to the variables quantified from the data collected. Total number of flora found at the study site is 31 in families, 44 in genera, and 56 in species. The main lower layer vegetation was investigated such as Jeju dwarf bamboo, Toothed club moss (Lycopodium serratum), Pacific fir moss (Lycopodium chinense), Climbing hydrangea (Hydrangea petiolaris) etc. Among these plant species, the vegetation which had a high positive correlation with the frequency and the coverage of seedlings of Korean fir were Jeju dwarf bamboo, Toothed club moss and Pacific fir moss.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.6
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• pp.71-82
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• 2018

This study was conducted to provide ecological basic data that use to establish environmental conditions for cultivation of wild vegetables in 2016-2018. Therefore, we investigated the vegetation structure and the correlation between the community structure and the environmental factors for natural habitats of wild vegetables(Aster glehni, Allium ochotense, and Aruncus sylvester) distributed in Ulleungdo. As a result of population and gradient analysis, the vegetation was classified into Aster glehni community, Allium ochotense community, and Aruncus sylvester community. We confirmed that the classification by population analysis was consistent with that by TWINSPAN method, suggesting that they were complemented each other. The importance value of Aster glehni was the highest in all communities, followed by Aruncus sylvester, Allium ochotense, Hydrangea petiolaris, Dryopteris crassirhizoma, Asperula ldorata, Phryma leptostachya var. asiatica, Disporum viridrescens, Hedera rhombea, Anthriscus sylvestris, and Hepatica maxima. According to the results of DCCA ordination analysis, among those communities, the Aster glehni community was distributed in soil where the nutrition including T-N and O.M. were intermediate. The Allium ochotense community was distributed on the a little high northern slope at the highest altitude where the CEC and O.M. were the highest, and other nutrition and pH were low. The Aruncus sylvester was distributed on high slope and altitude on which the amount of exchangeable cation such as $Ca^{{+}{+}}$, $Mg^{{+}{+}}$ and pH were high, and the CEC, $P_2O_5$, and O.M. were the lowest.

• Korean Journal of Environment and Ecology
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• v.26 no.5
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• pp.635-653
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• 2012

Vascular plants of Oruem in region of Jeju-City were investigated 454 taxa totally; 116 families, 301 genera, 359 species, 3 subspecies, 78 varieties and 14 forms. In the studied Oruems in region of Jeju-City within altitude 800m, evergreen tree forests does not range and a lot of Pines thunbergii forest and Cryptomeria japonica and Chamaecyparis obtusa plantation forest were. The endemic species of Oruem in region of Jeju-City were 14 taxa such as Asarum maculatum, Hepatica insularis, Cardamine glechomifolia etc. The floristics degree categorized by the specific distribution of plant species were total 116 Taxa, that is, V grade species Magnolia kobus, Aconitum japonicum subsp. napiforme, Ligularia taquetii etc, 6 kinds be, IV grade species Rubus ribisoideus, Ligustrum salicinum, Carex macrandrolepis etc, 16 kinds be, III grade species Illicium anisatum, Hydrangea petiolaris, Rubus schizostylus etc, 37 kinds be, II grade species Pseudostellaria coreana, potentilla dickinsii, Euonymus pauciflorus etc, 6 kinds and I grade species Sphenomeris chinensis, Pteris multifida, Machilus thunbergii etc, be. 51 kinds were confirmed. Rare and Endemic Plant Species of Korea Forest Service were 11 taxa such as Asarum maculatum, Aconitum japonicum subsp. napiforme and Magnolia kobus etc. Naturalized plant were confirmed 31 taxons with Rumex acetosella, Hypochaeris radicata and Sisyrinchium atlanticum etc.