• Polymer(Korea)
• /
• v.32 no.1
• /
• pp.26-30
• /
• 2008

This study was designed to investigate the effect of hybridization of synthetic/natural materials for annulus fibrosus (AF) tissue regeneration in vitro and in vivo. The synthetic/natural hybrid scaffolds were prepared using PLGA (poly (lactic-co-glycolic) acid), SIS (small intestinal submucosa) and DBP (demineralized bone particles). PLGA, PLGA/SIS(20%), PLGA/DBP(20%) and PLGA/SIS (10%)/DBP (10%) scaffold were manufactured by solvent casting/salt leaching method. Compressive strength was measured. Rabbit AF cells were isolated, cultured and seeded into experimental groups. Hydroxyproline production and DNA quantity of AP cells on each scaffold was measured at 2, 4 and 6 weeks after in vitro culture. Cell-scaffold composites were implanted subcutaneously into athymic mice. After 1,4 and 6 weeks postoperatively, specimens were taken and H&E, Safranin-O and type I collagen staining were carried out concerning formation of cartilagenous tissue. In vitro PLGA/SIS scaffold was evaluated for total collagen content (bydroryproline/DNA content) and PLGA scaffold was evaluated for compressive strength.

• Journal of the Korean Chemical Society
• /
• v.62 no.5
• /
• pp.377-385
• /
• 2018

A new class of Chalcone-Sulphonamide hybrids has been designed by condensing appropriate sulphonamide scaffold with substituted chalcones tethered by chloroacetyl chloride as a multi-target drug for therapeutic treatment. Chalcones were prepared by Claisen-Schmidt condensation of a substituted aldehyde with para aminoacetophenone. These Chalcone-Sulphonamide hybrids were screened by means of their antibacterial activity by NCCLS method. Among all these compounds, 5e and 5c displayed more potent growth inhibitory activity against Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria respectively. Further, these hybrids were evaluated for their antifungal activity, among all hybrid 5a exhibited potent antifungal activity. The synthesized compounds were characterized by FT-IR, $^1HNMR$, $^{13}CNMR$ and HR-LCMS and spectral study supports the structures of synthesized Chalcone-Sulphonamide hybrids.

• Journal of Veterinary Clinics
• /
• v.32 no.3
• /
• pp.224-230
• /
• 2015

This study was designed in the fabricated poly (L-Lactic-co-${\varepsilon}$-Caprolactone) (PLCL) scaffold using chitosan-alginate hydrogel, which would be more suitable to maintain the biological and physiological functions continuing three dimensional spatial organizations for chondrocytes. As a scaffold, hydrogels alone is weak at endure complex loading within the body. In this study, we made cell hybrid scaffold constructs with poly (L-Lactic-co-${\varepsilon}$-Caprolactone) (PLCL) scaffold and hydrogels to make a three-dimensional composition of cells and extracellular matrix, which would be a mimic of a native cartilage. Using a particle leaching technique with NaCl, we fabricated a highly-elastic scaffold from PLCL with 85% porosity and $300-500{\mu}m$ pore size. A mixture of bovine chondrocytes and chitosan-alginate gel was seeded and compared with alginate as a control on the PLCL scaffold. The cell maturation, proliferation, extracellular matrix synthesis, glycosaminoglycans (sGAG) production and collagen type-II expressions were better in chondrocytes seeded in chitosan-alginate hydrogel than in alginate only. These results indicate that chondrocytes with chitosan-alginate gel on PLCL scaffolds provide an appropriate biomimetic environment for cell proliferation and matrix synthesis, which could successfully be used for cartilage repair and regeneration.

• Journal of Sensor Science and Technology
• /
• v.29 no.3
• /
• pp.201-204
• /
• 2020

In this study, we fabricated and evaluated graphene composite based 3D scaffolds and planar films. The hybrid composite was prepared by mixing a calculated amount of graphene nanopowder and polydimethylsiloxane in tetrahydrofuran solution. The hybrid composite is easy to manufacture into various forms using direct printing technology or a pressing method. A 3D scaffold structure was prepared at ambient temperature with a flow rate of 240 mm/min. The nozzle pressure was maintained at 350 kPa by adjusting the viscosity of the composite material. The planar film was prepared at different thicknesses using a roll-to-roll equipment. The prepared hybrid nanocomposites were evaluated to investigate their electrical properties according to temperature and mechanical deformation. The obtained results were consistent with each other. Therefore, it can be used effectively as sensors through shape definition.

• Polymer(Korea)
• /
• v.30 no.1
• /
• pp.14-21
• /
• 2006

Tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which server as a three-dimensional template for initial cell attachment and subsequent tissue formation in both in vitro and in vivo. Small intestinal submucosa (SIS) has been investigated as a source of collagenous tissue with the potential to be used as biomaterials because of its inherent strength and biocompatibility. SIS-loaded poly(L-lactide-co-glicolide)(PLGA) scaffolds were prepared by solvent casting/particle leaching. Characterizations of SIS/PLGA scaffold were carried out by SEM, mercury porosimeter, and so on. Muscle-derived stem cells can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts by the controlling the culture environment. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. Osteogenic differential cells were analyzed by alkaline phosphatase(ALP) activity. SIS/PLGA scaffolds were implanted into the back of athymic nude mouse to observe the effect of SIS on the osteoinduction compared with controlled PLGA scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were conducted hematoxylin and eosin (H&E), Trichrome, and von Kossa. We observed that bone formatioin of SIS/PLGA hybrid scaffold as natural/synthetic scaffold was better thean that of only PLGA scaffold. It canb be explained that SIS contains various kinds of bioactive molecules for osteoinduction.

• Polymer(Korea)
• /
• v.32 no.5
• /
• pp.403-408
• /
• 2008

Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.

• Journal of Biomedical Engineering Research
• /
• v.38 no.5
• /
• pp.248-255
• /
• 2017

Polyurethane/polyethylene glycol(PU/PEG) hybrid scaffolds with various concentrations of PEG (0 to 50wt%) were prepared by electrospinning to evaluate the mechanical properties and the biocompatibility of the PU/PEG blend scaffolds. The 12wt% PU/PEG polymers were studied due to the absence of beads. The ultimate tensile strength of 12wt% PU was $8.2{\pm}0.5MPa$. The strength increased to $9.2{\pm}0.7MPa$ when 10% PEG was added to PU. However, the dry and the wet strength of PU/PEG scaffolds began to decrease dramatically when the PEG content was more than 10wt%. No cytotoxicity was observed for all the PU/PEG scaffolds investigated, indicating that the PU/PEG hybrid scaffolds are clinically safe and effective to small-diameter vascular grafts. In addition, the L-929 cells attached and proliferated well on the PU/PEG hybrid scaffolds.

• Journal of the Korean Ceramic Society
• /
• v.41 no.7
• /
• pp.497-502
• /
• 2004

A hybrid coextrusion and lamination process has been developed to fabricate macrochanneled bioceramic scaffolds. This process was mainly composed of three steps (i.e., coextrusion of thermoplastic compound, lamination, and thermal treatment), forming unique pore channels in dense bioceramic body. Pore channels were formed by removing carbon black material, while calcium phosphate or Tetragonal Zirconia Polycrystals (TZP) with a calcium phosphate coating layer were used as dense body. Two kinds of pore structures were fabricated; that is, the pore channels were formed in uni- or three-directional array. Such macrochanneled bioceramic scaffolds exhibited the precisely controlled pore structure (pore size, porosity, and interconnection), offering excellent mechanical properties and cellular responses.

• Polymer(Korea)
• /
• v.35 no.3
• /
• pp.189-195
• /
• 2011

The design of new bioactive scaffolds offering physiologic environment for tissue formation is an important frontier in biomaterials research. In this study, we performed compressive strength, water-uptake ability, and SEM analysis for physical property assessment of 3-D silk/PLGA scaffold, and investigated the adhesion, proliferation, phenotype maintenance, and inflammatory responses of RAW 264.7 and NIH/3T3 for cell compatibility. Scaffolds were prepared by the solvent casting/salt leaching method and their compressive strength and water-uptake ability were excellent at 20 wt% silk content. Result of cell compatibility assay showed that inflammatory responses distinctly decreased, and initial adhesion and proliferation were maximized at 20 wt% silk content. In conclusion, we suggest that silk/PLGA scaffolds may be useful to tissue engineering applications.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.4
• /
• pp.531-540
• /
• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.