• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.65-71
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• 2003

Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorho-damine 123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.3 s.116
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• pp.120-138
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• 2006

A city evolves over time. It grows, transforms, and sometimes degrades. Chuncheon is at a turning point from a city souggling with regulations regarding clean water supply and a military encampment to a masterpiece city with a sustainable vision. The city is getting ready to restructure itself to become a world-famous culture and tourism complex expanding its physical boundary across the Camp Page site and absorbing Jungdo as a major tourist attraction. The landscape in the future blueprint of Chuncheon will play a great role in restructuring urban form. The regenerated in will have a new networked open space system as well as re-evaluated landscape resources. The hybrid theoretical practice called 'landscape urbanism' burgeoning in the fields between 'landscape architecture' and 'urbanism' can guide us in considering the terms of the relationship between a city and landscape when we design a future city Landscape urbanism is considered to be an effective framework by which we can diagnose the current status of a landscape in our contemporary urban design practice in Korea. This paper tries to provide a different perspective from the viewpoint of landscape urbanism to decipher the hidden implications of the social agreement on the role of landscape in urban structure by re-reading eight design proposals presented for the ChunCheon G5 international design competition based on the main principles of landscape urbanism. The G5 design competition is a great opportunity to test out new ideas on a city, demonstrating the relative values among various urban-design professional realms. First, this paper provides an overview of the main ideas of landscape urbanism based on the literature review and case studies. Second, framework categories are suggested in order to extract the explicit and implicit ideas on the landscape. Third, eight proposals are reviewed according to the suggested categories to situate the current landscape design of Korea within the mainstream of contemporary practice of landscape urbanism. Based on the review of eight proposals, the following diagnostic conclusions are made; first, the ideas of landscape urbanism have not been actively introduced in large-scaled urban landscape projects in Korea like Chuncheon G5. Second, it remains to be a big task for landscape professions to be able to participate in design consortiums on an equal footing. Third, In order to introduce and reify the ideas of landscape urbanism in Korea, it is inevitable and critical to test the ideas in both academic fields and professional practices to find the appropriately adjusted model of landscape urbanism.

• Journal of Korean Society of Transportation
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• v.23 no.3 s.81
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• pp.35-47
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• 2005

This paper is to review a literature concerning Bus Transit Route Network Design(BTRNDP), to describe a future study direction for a systematic application for the BTRNDP. Since a bus transit uses a fixed route, schedule, stop, therefore an approach methodology is different from that of auto network design problem. An approach methodology for BTRNDP is classified by 8 categories: manual & guideline, market analysis, system analytic model. heuristic model. hybrid model. experienced-based model. simulation-based model. mathematical optimization model. In most previous BTRNDP, objective function is to minimize user and operator costs, and constraints on the total operator cost, fleet size and service frequency are common to several previous approach. Transit trip assignment mostly use multi-path trip assignment. Since the search for optimal solution from a large search space of BTRNDP made up by all possible solutions, the mixed combinatorial problem are usually NP-hard. Therefore, previous researches for the BTRNDP use a sequential design process, which is composed of several design steps as follows: the generation of a candidate route set, the route analysis and evaluation process, the selection process of a optimal route set Future study will focus on a development of detailed OD trip table based on bus stop, systematic transit route network evaluation model. updated transit trip assignment technique and advanced solution search algorithm for BTRNDP.

• Journal of the Korean Electrochemical Society
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• v.2 no.4
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• pp.218-220
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• 1999

The performance of the Electric Vehicle and Hybrid Electric Vehicle depends on that of the battery pack composed of series connected batteries. And thermal property is one of the main factors which decide the performance of the battery pack. So heat generation rate from the battery under the various driving mode must be measured as precise as possible because thermal characteristics of the battery affect the driving performance and battery pack's life cycle. Besides, to design and develop the battery thermal management system for the EV and HEV, the measurements of the thermal properties of the batteries are needed. However, the established calorimeter is not adequate to test an EV's battery because its cavity is too small to accommodate the EV's battery. Therefore we developed the calorimeter to test the thermal property of the EV's battery. Its cavity size is 120mm long, 75mm wide and 200mm high. The calorimeter is calibrated by the dummy cell which generates the heat rate from zero to 200W. The measuring accuracy of the calorimeter is within $2\%$ and its voltage stability is 2.5mV in the constant temperature bath.

• Journal of Intelligence and Information Systems
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• v.29 no.1
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• pp.143-174
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• 2023

With the development of deep learning technologies, Artificial Intelligence powered Optical Character Recognition (AI-OCR) has evolved to read multiple languages from various forms of images accurately. For the financial industry, where a large number of diverse documents are processed through manpower, the potential for using AI-OCR is great. In this study, we present a configuration and a design of an AI-OCR modality for use in the financial industry and discuss the platform construction with application cases. Since the use of financial domain data is prohibited under the Personal Information Protection Act, we developed a deep learning-based data generation approach and used it to train the AI-OCR models. The AI-OCR models are trained for image preprocessing, text recognition, and language processing and are configured as a microservice architected platform to process a broad variety of documents. We have demonstrated the AI-OCR platform by applying it to financial domain tasks of document sorting, document verification, and typing assistance The demonstrations confirm the increasing work efficiency and conveniences.