• BMB Reports
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• 제35권6호
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• pp.562-567
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• 2002

In order to find the cellular interaction factors of the Heliothis armigera nuclear polyhedrosis virus capsid protein VP39, a Heliothis armigera cell cDNA library was constructed. Then VP39 was used as bait. The host actin gene was isolated from the cDNA library with the yeast two-hybrid system. This demonstrated that VP39 could interact with its host actin in yeast. In order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pEGFP39. The fusion protein was expressed in the Hz-AM1 cells under the control of the Autographa californica multiple nucleopolyhedrovirus immediate early gene promoter. The host actin was labeled specifically by the red fluorescence substance, tetramethy rhodamine isothicyanete-phalloidin. Observation under a fluorescence microscopy showed that VP39, which was indicated by green fluorescence, began to appear in the cells 6 h after being transfected with pEGFP39. Red actin cables were also formed in the cytoplasm at the same time. Actin was aggregated in the nucleus 9 h after the transfection. The green and red fluorescence always appeared in the same location of the cells, which demonstrated that VP39 could combine with the host actin. Such a combination would result in the actin skeleton rearrangement.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2001년도 추계학술대회논문집 I
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• pp.427-432
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• 2001

This paper shows the optimum design of the fluid engine mount. The design has been modified by trial and error because there is many design parameters that can be varied in order to obtain resonant and notch frequencies, and notch depth. It seems to be a great application for optimal design for the mount. Many combinations of parameters are possible to give us the desired resonant and notch frequencies, but the question is which combination provides the lowest resonant peak and notch depth\ulcorner In this study, the enhanced artificial life algorithm is applied to get the desired notch frequency of a fluid mount and minimize transmissibility at the notch frequency. The present hybrid algorithm is the synthesis of an artificial life algorithm with the random tabu (R-tabu) search method. The hybrid algorithm has some advantages, which is not only faster than the conventional artificial life algorithm, but also gives a more accurate solution. In addition, this algorithm can find all global optimum solutions. The results show that the performance of a conventional engine mount can be improved significantly compared with the optimized mount.

• 전기학회논문지P
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• 제57권3호
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• pp.338-342
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• 2008

Hybrid Electric Vehicle(HEV) is driven by an internal-combustion engine and an electric motor. It is a combination of an internal-combustion engine and several electrical equipments which use a high voltage battery, an electric motors, an inverter and others. But there is not any separate detailed enforcement regulations for high voltage electric appliances in the existing vehicle-related safety standards. So, test standards suggestion as well as test technique development need to be done for ensuring electrical safety, for an electric motor, a high voltage battery, a(n) inverter/converter and an electric power transmission units and other equipments to ensure the safety of high voltage electric appliances which is the HEV key electrical component. In this paper, We are to provide helpful data to support test technique development and test standard establishment for HEV design and electrical safety security by the following methods; by measuring the voltage, the electric current, and the frequency of HEV, by analyzing electrical characteristics of high voltage electric appliances, and by analyzing temperature characteristics of the electrical current among the analyzed electrical characteristics by thermal imagining cameras.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권3호
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• pp.560-574
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• 2011

In this paper, we propose a hybrid method of Machine Learning (ML) algorithm and a rule-based algorithm to implement a lightweight Named Entity (NE) extraction system for Korean SMS text. NE extraction from Korean SMS text is a challenging theme due to the resource limitation on a mobile phone, corruptions in input text, need for extension to include personal information stored in a mobile phone, and sparsity of training data. The proposed hybrid method retaining the advantages of statistical ML and rule-based algorithms provides fully-automated procedures for the combination of ML approaches and their correction rules using a threshold-based soft decision function. The proposed method is applied to Korean SMS texts to extract person's names as well as location names which are key information in personal appointment management system. Our proposed system achieved 80.53% in F-measure in this domain, superior to those of the conventional ML approaches.

• 한국소음진동공학회논문집
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• 제12권8호
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• pp.598-608
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• 2002

This paper shows the optimal design methodology for the fluid engine mount by the artificial life algorithm. The design has been commonly modified by trial and error because there is many design parameters that can be varied in order to minimize transmissibility at the desired fundamental resonant and notch frequencies. The application of trial and error method to optimization of the fluid mount is a great work. Many combinations of parameters are possible to give us the desired resonant and notch frequencies, but the question is which combination Provides the lowest resonant peak and notch depth. In this study the enhanced artificial life algorithm is applied to get the desired fundamental resonant and notch frequencies of a fluid mount and to minimize transmissibility at these frequencies. The present hybrid algorithm is the synthesis of and artificial life algorithm with the random tabu (R-tabu) search method. The hybrid algorithm has some advantages, which is not only faster than the conventional artificial life algorithm, but also gives a more accurate solution. In addition, this algorithm can find all globa1 optimum solutions. The results show that the performance of the optimized mount compared with the original mount is improved significantly.

• 제어로봇시스템학회논문지
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• 제6권9호
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• pp.796-802
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• 2000

A master-slave system for teleoperation is usually used to control the robor's motion on remote place such as abyss, outer space etc.. When the slave robot is a humanoid one, it can make a better performance if the configuration of the master arm is similar to that of the slave arm and of the human. The master arm proposed in this paper has a type to be put on the human arm, that is, the exoskeleton type, and has a combination of serial joint and parallel mechanism imitating the human's arm structure of muscles and bones, so called hybrid mechanism so that it can follow arm's movement effectively. But it is easy to solve the forward kinematis of the parallel structure because relating equations are implicit functions. In order to solve that, the virtual joint angle corresponding to human arm's joint is introduced and a sequential computation step is employed in calculating virtual joint angles and the posture of the end effector. Also validity is checked up through computational simulation.

• Journal of Electrical Engineering and Technology
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• 제8권2호
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• pp.215-222
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• 2013

Electrical power distribution systems are critical links between the utility and customer. In general, power distribution systems have unbalanced feeders due to the unbalanced loading. The devices that dependent on balanced three phase supply are affected by the unbalanced feeders. This necessitates the balancing of feeders. The main objective of reconfiguration is to balance the loads among the phases subject to constraints such as load flow equations, capacity and voltage constraints and to reduce the real power loss, while subject to a radial network structure in which all loads must be energized. Therefore, the distribution system reconfiguration problem has been viewed as multi-objective problem. In this paper, the hybrid heuristic algorithm has been used for reconfiguration, which is the combination of fuzzy and greedy algorithms. The purpose of the introduction of greedy is to refrain the searching for the period of phase balancing. The incorporation of fuzzy helps to take up more objectives amid phase balancing in the searching. The effectiveness of the proposed method is demonstrated through modified IEEE 33 bus and modified IEEE 125 bus radial distribution system.

• Smart Structures and Systems
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• 제25권6호
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• pp.765-778
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• 2020

The present study intends to find a proper solution for the estimation of the physical behaviors of enlarged piles through a combination of small-scale laboratory tests and a hybrid computational predictive intelligence process. In the first step, experimental program is completed considering various critical influential factors. The results of the best multilayer perceptron (MLP)-based predictive network was implemented through three mathematical-based solutions of dragonfly algorithm (DA), whale optimization algorithm (WOA), and ant lion optimization (ALO). Three proposed models, after convergence analysis, suggested excellent performance. These analyses varied based on neurons number (e.g., in the basis MLP hidden layer) and of course, the level of its complexity. The training R² results of the best hybrid structure of DA-MLP, WOA-MLP, and ALO-MLP were 0.996, 0.996, and 0.998 where the testing R² was 0.995, 0.985, and 0.998, respectively. Similarly, the training RMSE of 0.046, 0.051, and 0.034 were obtained for the training and testing datasets of DA-MLP, WOA-MLP, and ALO-MLP techniques, while the testing RMSE of 0.088, 0.053, and 0.053, respectively. This obtained result demonstrates the excellent prediction from the optimized structure of the proposed models if only population sensitivity analysis performs. Indeed, the ALO-MLP was slightly better than WOA-MLP and DA-MLP methods.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2006년도 춘계학술대회 논문집
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• pp.321-326
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• 2006

This paper is proposed an efficiency optimization control algorithm for a synchronous reluctance motor which minimizes the copper and iron losses. The design of the speed controller based on adaptive fuzzy-neural networks(AFNN) controller that is implemented using fuzzy control and neural networks. There exists a variety of combinations of d and q-axis current which provide a specific motor torque. The objective of the efficiency optimization controller is to seek a combination of d and q-axis current components, which provides minimum losses at a certain operating point in steady state. It is shown that the current components which directly govern the torque production have been very well regulated by the efficiency optimization control scheme. The proposed algorithm allows the electromagnetic losses in variable speed and torque drives to be reduced while keeping good torque control dynamics. The control performance of the hybrid artificial intelligent controller is evaluated by analysis for various operating conditions. Analysis results are presented to show the validity of the proposed algorithm.

• Journal of Power Electronics
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• 제17권3호
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• pp.704-715
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• 2017

For three-phase synchronization applications, the synchronous reference frame phase-locked loop (SRF-PLL) is probably the most widely used technique due to its ease of implementation and satisfactory phase tracking performance under ideal grid conditions. However, under unbalanced and distorted grid conditions, its performance tends to worsen. To deal with this problem, a variety of filtering stages have been proposed and used in SRF-PLLs for the rejection of disturbance components at the cost of degrading the dynamic performance. In this paper, to improve dynamic performance without compromising the filtering capability, an effective hybrid filtering stage is proposed and incorporated into the inner loop of a quasi-type-1 PLL (QT1-PLL). The proposed filtering stage is a combination of a moving average filter (MAF) and a modified delay signal cancellation (DSC) operator in cascade. The time delay caused by the proposed filtering stage is smaller than that in the conventional MAF-based and DSC-based PLLs. A small-signal model of the proposed PLL is derived. The stability is analyzed and parameters design guidelines are given. The effectiveness of the proposed PLL is confirmed through experimental results.