• Microbiology and Biotechnology Letters
• /
• v.47 no.2
• /
• pp.278-288
• /
• 2019

In the present investigation, we attempted to design a protocol to develop a hybrid protein with better bioremediation capacity. Using in silico approaches, a Hybrid Open Reading Frame (Hybrid ORF) is developed targeting the genes of microorganisms known for degradation of organophosphates. Out of 21 genes identified through BLAST search, 8 structurally similar genes (opdA, opd, opaA, pte RO, pdeA, parC, mpd and phnE) involved in biodegradation were screened. Gene conservational analysis categorizes these organophosphates degrading 8 genes into 4 super families i.e., Metallo-dependent hydrolases, Lactamase B, MPP and TM_PBP2 superfamily. Hybrid protein structure was modeled using multi-template homology modeling (3S07_A; 99%, 1P9E_A; 98%, 2ZO9_B; 33%, 2DXL_A; 33%) by $Schr{\ddot{o}}dinger$ software suit version 10.4.018. Structural verification of protein models was done using Ramachandran plot, it was showing 96.0% residue in the favored region, which was verified using RAMPAGE. The phosphotriesterase protein was showing the highest structural similarity with hybrid protein having raw score 984. The 5 binding sites of hybrid protein were identified through binding site prediction. The docking study shows that hybrid protein potentially interacts with 10 different organophosphates. The study results indicate that the hybrid protein designed has the capability of degrading a wide range of organophosphate compounds.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.31 no.2
• /
• pp.127-134
• /
• 2011

This experiment was carried out to know the adaptability and forage production and quality of corn hybrid for silage at paddy field of Chungnam region from 2008 to 2009. Among agronomic characteristics, 'Kangdaok' and 'Kwangpyongok' hybrid were somewhat strong for waterlogging and good stay green, lodging, disease and insect resistance. The dry matter yield of 'Kangdaok' and 'Kwangpyongok' hybrid were the highest among korean corn hybrids. Among introduced hybrid, 'P32T83' hybrid was strong for lodging and insect resistance, and had lower ear height and high Brix ($B^o$, %). The dry matter yield of 'P32T83' and 'P32P75' hybrid were 20,150 kg/ha and 20,500 kg/ha, respectively. The 'P32P75' hybrid was high as 73% in vitro dry matter digestibility (IVDMD) and good stay green. The result of this study indicated that 'Kangdaok', 'Kwangpyongok', 'P32T83' and 'P32P75' hybrid could be recommended as having good characters according to production, waterlogging tolerance, stay green, disease and insect resistance at paddy field in the middle region of Korea.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.31 no.2 s.257
• /
• pp.166-173
• /
• 2007

Fatigue fracture behavior of a hybrid bolted joint was evaluated in comparison to the case of static fracture. Two kinds of specimens were fabricated for the mechanical tests; a hybrid bolted joint specimen for the shear test and a hybrid joint part specimen applied in the real tilting car body for the bending test. Characteristic fracture behaviors of those specimens under cyclic toads were obviously different from the case under static loads. For the hybrid bolted joint specimen, static shear loading caused the fracture of the bolt body itself in a pure shear mode, whereas cyclic shear loading brought about the fracture at the site of local tensile stress concentration. For the hybrid joint part specimen, static bend loading caused the shear deformation and fracture in the honeycomb core region, while cyclic bend loading did the delamination along the interface between composite skin and honeycomb core layers as well as the fracture of welded joint part. Experimental results obtained by static and fatigue tests were reflected in modifications of design parameters of the hybrid joint structure in the real tilting car body.

• Metals and materials international
• /
• v.24 no.6
• /
• pp.1346-1358
• /
• 2018

A novel additive manufacturing method with TIG-MIG hybrid heat source was applied for fabricating 5356 aluminum alloy component. In this paper the microstructure evolution, mechanical properties and fracture morphologies of both as-deposited and heat-treated component were investigated, and how these were affected by different heat-treated temperature. The as-deposited microstructure showed dominant equiaxed grains with second phase, and the size of them is coarse in the bottom region, medium in the middle region and fine in the top region owing to different thermal cycling conditions. Compared with as-deposited microstructure, the size of grain becomes large and second phases gradually dissolve in the matrix as heat-treated temperature increase. Different microstructures determine the mechanical properties of component. Results show that average ultimate tensile strength enhances from 226 to 270 MPa and average microhardness increases from 64.2 to 75.3 HV0.1 but ductility decreases from 33 to 6.5% with heat-treated temperature increasing. For all components, the tensile properties are almost the same in the vertical direction (Z) and horizontal direction (Y) due to equiaxed grains, which exhibits isotropy, and the mechanisms of these are analyzed in detailed. In general, the results demonstrate that hybrid arc heat source has the potential to fabricate aluminum alloy component.

• Journal of the Korean Institute of Telematics and Electronics B
• /
• v.32B no.12
• /
• pp.1652-1662
• /
• 1995

In this paper, a hybrid neural network is proposed to improve the learning ability of a neural network. The union of the characteristics of a Self-Organizing Neural Network model and of multi-layer perceptron model using the backpropagation learning method gives us the advantage of reduction of the learning error and the learning time. In learning process, the proposed hybrid neural network reduces the number of nodes in hidden layers to reduce the calculation time. And this proposed neural network uses the fuzzy feedback values, when it updates the responding region of each node in the hidden layer. To show the effectiveness of this proposed hybrid neural network, the boolean function(XOR, 3Bit Parity) and the solution of inverse kinematics are used. Finally, this proposed hybrid neural network is applied to the visual tracking control of a PUMA560 robot, and the result data is presented.

• Journal of Institute of Control, Robotics and Systems
• /
• v.8 no.2
• /
• pp.89-96
• /
• 2002

A new chattering free PD-sliding mode hybrid control scheme is proposed for robot manipulators. This hybrid controller is composed of a PD controller and a semi-continuous sliding mode controller. It has a good robust performance in reaching mode which does not possess invariance property of sliding mode, and has chattering free characteristics in sliding mode. Thus, the PD-sliding mode hybrid controller has a good robust performance in the whole region. It is shown that the proposed control has a good transient response and trajectory tracking performance for a 2-link SCARA robot manipulator.

• Journal of Electrical Engineering and Technology
• /
• v.9 no.5
• /
• pp.1582-1591
• /
• 2014

This paper presents a hybrid sensorless control for an interior permanent magnet synchronous motor (IPMSM) for zero-, low-, and high-speed regions. Many sensorless control methods such as an observer-based estimator have been introduced. However, most of the observer-based estimators have a disadvantage at start-up and in the low-speed region. To solve this problem, a simple strategy of using a hybrid system is proposed by integrating a high-frequency (HF) signal injection method and a full-order flux observer. In addition, an HF signal injection method with only a low pass filter (LPF) is proposed to simplify the hybrid system. The hybrid system achieves high-performance drive throughout the entire speed range. The effectiveness of the proposed hybrid technique is verified by experiments using an 11-kW IPMSM drive system.

• The Plant Pathology Journal
• /
• v.28 no.1
• /
• pp.75-80
• /
• 2012

$Potato$ $virus$ $X$ (PVX) contains $cis$-acting elements including stem-loop 1 (SL1) RNA at the 5' region; SL1 is conserved among all potexviruses. The SL1 at the positive-sense RNA, SL1(+), is required for PVX RNA replication, cell-to-cell movement, and translation. Previous research demonstrated that SL1(+) RNA also serves as the origin of assembly for encapsidation of PVX RNA. To identify the essential sequences and/or regions for capsid protein (CP) subunit recognition within SL1(+) RNA, we used electrophoretic mobility shift assays (EMSA), UV cross-linking, and yeast three-hybrid analyses. The EMSA and UV cross-linking analyses with PVX CP subunits and RNA transcripts corresponding to the SL1(+) RNA showed that the SL1(+) RNA formed complexes with CP subunits. We also conducted EMSA and yeast three-hybrid analyses with RNAs containing various mutations of SL1(+) RNA elements. These analyses indicated that SL1(+) RNA is required for the interaction with PVX CP and that the RNA sequences located at the loop C and tetra loop of the SL1(+) are crucial for CP binding. These results indicate that, in addition to being important for RNA accumulation, the SL1(+) RNA from the 5' region of the PVX genome is also required for specific binding of PVX CP.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.22 no.12
• /
• pp.1657-1668
• /
• 1998

In the analysis of a mushy solidification system with natural convection using a fixed grid method, the enthalpy method has been used to account for the release of latent heat. The variable viscosity, Darcy source, and hybrid methods have been employed for the velocity suppression in a mushy region. The choice of the values of solid viscosity and permeability constant in conjunction with the Darcy source term plays an important role in forming the location and shape of the phase boundaries. In this work the effects of these major parameters related to steady-state behavior in the system of mushy solidification are investigated through a simple test problem. The effective specific heat based on the spatial gradients of the enthalpy and temperature is adopted for the treatment of the release of latent heat. The effects of the Prandtl and Rayleigh numbers on the shape of mushy region are examined using the hybrid method.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.22 no.3
• /
• pp.83-91
• /
• 2022

This paper proposes a scalable hybrid P2P(peer-to-peer) cloud architecture for MMOGs(massively multiplayer online games) which includes load management schemes for each region. A game world is divided into several game regions and each game region is serviced by at least one peer(player) in this MMOG cloud environment. The load must be managed regionally to support smooth interactions among them even in the presence of a high concentration of players in a specific region. In the proposed architecture where an efficient and effective provisioning of resources is realized, it is suitable for players to interact with cloud servers effectively and it avoids bottlenecks of the current client-server MMOG architecture. This architecture also relieves a lot of computational power and network traffic, the load on the servers in the cloud by exploiting the capacity of the players. Simulation results show that the proposed hybrid P2P cloud architecture can reduce the considerable bandwidth at the server compared to the client server architecture as the available resources grow with the number of players in crowding or hotspots.