• The Journal of Korean Society of Virology
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• v.30 no.3
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• pp.211-217
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• 2000

Nef is a lentiviral protein involved in pathogenesis of AIDS, but its molecular mechanism of action remains incompletely understood. Here we report the isolation of the interacting protein with the HIV-1 Nef, using the yeast two hybrid system for expression cloning. One of the positive colonies was selected as the final candidate for the interacting protein gene. The nucleotide sequencing revealed that this interacting protein is Human Ikaros/LyF-1. This protein interacted with the C-terminal region of Nef specifically in yeast system, not with the N-terminal region. This interaction was also confirmed by in vitro binding assay.

• Journal of Integrative Natural Science
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• v.1 no.1
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• pp.47-53
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• 2008

In a previous study, we showed that Cecropin A (1-8)-Magainin 2 (1-12) hybrid peptide (CA-MA)'s analogue, Anal P5, exhibit broad-spectrum antimicrobial activity. Anal P5, designed by flexible region (positions 9, 10)-substitution, Lys- (positions 4, 8, 14, 15) and Leu- (positions 5, 6, 12, 13, 16, 17, 20) substitutions, showed an enhanced antimicrobial and antitumor activity without hemolysis. The primary objective of the present study was to gain insight into the relevant mechanisms of antimicrobial activities of Anal P5 by using flow cytometric analysis. Anal P5 exhibits strong antifungal activity in a salt concentration independent manner. In addition, Anal P5 causes significant morphological alterations of the bacterial surfaces as shown by scanning electron microscopy, supporting its antibacterial activity. Its potent antibiotic activity suggests that Anal P5 is an excellent candidate as a lead compound for the development of novel antibiotic agents.

• Journal of Electrical Engineering and Technology
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• v.7 no.3
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• pp.288-296
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• 2012

The method proposed by the author is intended for assistance in decision-making (concerning changes of connections) by operators of complex distribution systems during states of malfunction (particularly in the events of malfunctions, for which the consequences encompass extended parts of the network), through designation of connection action scenarios (creating substitute configurations). It is the use by the classifying system working with the co-evolution algorithm that enables the effective creation of substitute scenarios for the Medium Voltage electric power distribution network. The author also completed works concerning the possibility of using cooperation of the evolutionary algorithm and the co-evolutionary algorithm with local search algorithms. The method drawn up may be used in current systems managing the work of distribution networks to assist network operators in taking decisions concerning connection actions in supervised electric power systems.

• Journal of the Earthquake Engineering Society of Korea
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• v.5 no.1
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• pp.63-72
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• 2001

상부 벽체와 하부 골조로 구성되는 주상복합건물은 전이층에서 수직적인 강성과 강도의 불연속성을 갖는다. 이러한 복합구조는 전이층에서 춤이 큰 보에 의하여 하중이 전달되면 설계시 매우 중요하게 고려하여야 하는 사항이다. 그러나 이에 대한 연구가 충분히 이루어져 있지 않으며 실제 전이보의 설계시 춤을 매우 크게 하여 요구되는 강도보다 큰 안전율이 고려되고 있다. 본 연구에서는 전이층의 단순화모델을 이용하여 보의 높이 및 지지면의 길이에 따른 아치거동의 변화를 조사하였다. 유한요소법을 이용하여 구조물을 분석하고 두 변수를 포함하는 헌치부재를 이용하여 그 효과를 기존 시스템과 비교하였다. 중요 변수와 헌치의 기울기는 1:1의 비율에서 사장 효율이 좋은 것으로 나타났으며, 이러한 결과를 전이보를 대신하여 사용한 결과 중력방향의 상부 아치거동에 대하여 효과적으로 작용하는 것으로 나타났다. 또한 응력 집중부위에서의 응력 감소와 깊이 전이보의 높이감소에 효과적으로 작용하는 것으로 나타났다.

• Journal of Korean Port Research
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• v.7 no.1
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• pp.79-88
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• 1993

This study deals with the case of a fixed floating structure(FFS) at the mouth of a rectangular harbor under the action of waves represented by the linear wave theory. Modified forms of the mild-slope equation is applied to the propagation of regular wave over constant water depth. The model is extended to include bottom friction and boundary absorption. A hybrid element approximation is used for calculation of linear wave oscillation in and near coastal harbor. Modification of the model was necessary for the FFS. For the conditions tested, the results of laboratory experiments by Ippen and Goda(1963), and Lee (1969) are compared with the calculated one from this model. The cases of flat cylinderical structures, both fixed and floating, were taken to be in an intermediate water depth.

• Journal of information and communication convergence engineering
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• v.19 no.4
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• pp.214-220
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• 2021

In this study, we constructed several scenarios that are required for LED lighting, and we designed and implemented an LED lighting control system to operate these scenarios to confirm their behavior. An LED lighting control system is a hybrid control board that is designed by combining LED controllers and SMPS, consisting of an AC/DC power supply part that converts AC 220 V into DC 12 V, and a drive and control part that controls the scenario and color of the LED module. Conventional LED light controllers have an input power of DC 12 V, so when using the input AC 220 V, the SMPS must be connected to the LED light controller. To eliminate this inconvenience, a hybrid LED lighting control system was configured to combine LED lighting controllers and SMPS into one control system. Furthermore, we designed a control system to represent the most appropriate color according to the input of the distance and illumination using a fuzzy control system to conduct computer simulations.

• Journal of Korea Multimedia Society
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• v.22 no.12
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• pp.1457-1465
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• 2019

3D convolution is to stack multiple consecutive frames to form a cube, and then apply the 3D convolution kernel in the cube. In this structure, each feature map of the convolutional layer is connected to multiple adjacent sequential frames in the previous layer, thus capturing the motion information. However, due to the changes of pedestrian posture, motion and position, the convolution at the same place is inappropriate, and when the 3D convolution kernel is convoluted in the time domain, only time domain features of three consecutive frames can be extracted, which is not a good enough to get action information. This paper proposes an action recognition method based on feature fusion of 3D convolutional neural network. Based on the VGG16 network model, sending a pre-acquired optical flow image for learning, then get the time domain features, and then the feature of the time domain is extracted from the features extracted by the 3D convolutional neural network. Finally, the behavior classification is done by the SVM classifier.

• Steel and Composite Structures
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• v.34 no.5
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• pp.715-732
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• 2020

High-strength steel composite Y-eccentrically braced frame (Y-HSS-EBF) is a novel structural system. In this study, the spatial substructure hybrid simulation test (SHST) method is used to further study the seismic performance of Y-HSS-EBF. Firstly, based on the cyclic loading tests of two single-story single-span Y-HSS-EBF planar specimens, a finite element model in OpenSees was verified to provide a reference for the numerical substructure analysis model for the later SHST. Then, the SHST was carried out on the OpenFresco test platform. A three-story spatial Y-HSS-EBF model was taken as the prototype, the top story was taken as the experimental substructure, and the remaining two stories were taken as the numerical substructure to be simulated in OpenSees. According to the test results, the validity of the SHST was verified, and the main seismic performance indexes of the SHST model were analyzed. The results show that, the SHST based on the OpenFresco platform has good stability and accuracy, and the results of the SHST agree well with the global numerical model of the structure. Under strong seismic action, the plastic deformation of Y-HSS-EBF mainly occurs in the shear link, and the beam, beam-columns and braces can basically remain in the elastic state, which is conducive to post-earthquake repair.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.4
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• pp.201-208
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• 2003

The Hybrid Wall System(HWS) building composed of center core reinforced concrete walls and exterior steel frame has open space around the center core walls. It is necessary to develop design methodologies for the HWS building that the coupled shear walls withstand the most of lateral load and expect the most energy dissipation at the coupling beams and at wall foots. Major factors considered in this paper are connection type of coupling beams and scale of story. The studies of the system are investigated in terms of shear force, overturning moment, maximum lateral displacement, story drift ratio, and dynamical characteristics under the action of vertical and lateral forces such as wind and seismic loads.

• Computers and Concrete
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• v.31 no.3
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• pp.197-206
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• 2023

In this paper, the bending performance of a MSFRHPC (containing steel fiber, polyvinyl alcohol (PVA) fiber, and CW)-reinforced beam was studied for the first time. Introducing a multiscale fiber system increased the first crack load (up to 150%), yield load (up to 50%), and peak load (up to 15%) of reinforced beams. The multiscale fiber system delays cracking of the reinforced beam, reduces crack width of the reinforced beam in normal use, and improves the durability of the beam. Considering yield load and peak load, the reinforcing effect of multiscale fiber on the high-reinforcement ratio beam (1.00%) is better than that on the low-reinforcement ratio beam (0.57%). Introducing fibers slowed the development of cracks in the reinforced beam under bending. With the added hybrid fiber, the deformation concentration of reinforced beams after yield was more significant with concentration in 1 or 2 cracks. A model for predicting the flexural capacity of MSFRHPC-reinforced beams was proposed, considering the action of multiscale hybrid fibers. This research is helpful for structure application of MSFRHPC-containing CW.