The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 30 Issue 3
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- Pages.211-217
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- 2000
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- 1225-2344(pISSN)
Interaction between HIV-1 Nef and LyF-1, the T Cell Specific Transcription Factor
T 세포 특이적 전사인자인 LyF-1과 HIV-1 Nef의 상호 작용
- Lee, Mi-Seon (Bio-Med RRC and Division of Life Science, Pai-Chai University) ;
- Lee, Kyoung-Hoa (Bio-Med RRC and Division of Life Science, Pai-Chai University) ;
- Kim, Jung-Woo (Bio-Med RRC and Division of Life Science, Pai-Chai University)
- 이미선 (배재대학교 자연과학대학 생명과학부, 바이오의약 RRC) ;
- 이경화 (배재대학교 자연과학대학 생명과학부, 바이오의약 RRC) ;
- 김정우 (배재대학교 자연과학대학 생명과학부, 바이오의약 RRC)
- Published : 2000.09.30
Abstract
Nef is a lentiviral protein involved in pathogenesis of AIDS, but its molecular mechanism of action remains incompletely understood. Here we report the isolation of the interacting protein with the HIV-1 Nef, using the yeast two hybrid system for expression cloning. One of the positive colonies was selected as the final candidate for the interacting protein gene. The nucleotide sequencing revealed that this interacting protein is Human Ikaros/LyF-1. This protein interacted with the C-terminal region of Nef specifically in yeast system, not with the N-terminal region. This interaction was also confirmed by in vitro binding assay.